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Nitrogenous Wastes
Nitrogenous Wastes

... to urea, as shown in Figure 1. The amino acid L-ornithine gets converted into dierent intermediates before being regenerated at the end of the urea cycle. Hence, the urea cycle is also referred to as the ornithine cycle. The enzyme ornithine transcarbamylase catalyzes a key step in the urea cycle a ...
Automatic De Novo Sequencing of peptides by Electron Transfer
Automatic De Novo Sequencing of peptides by Electron Transfer

... the intact precursor (Figure 2). Hence, ETD spectra carry little information codified on the ion abundances. In contrast, CID product ions have very different abundances that depend on the neighboring amino acids and other characteristics of the peptide sequence. Nevertheless, abundances of ions of ...
This article was published in an Elsevier journal. The attached copy
This article was published in an Elsevier journal. The attached copy

... 2000). A defective lambda prophage supplies the function that protects and recombines linear DNA. This system is highly efficient and allows recombination between homologies as short as 40 bp. The first step in making each VZV ORF deletion was to amplify the KanR cassette containing 40-bp flanking s ...
Coevolution theory of the genetic code at age thirty
Coevolution theory of the genetic code at age thirty

... similar amino acids at neighboring positions (one base removed from each other) in the code would minimize the damage due to mutations, and errors of transcription and translation. The genetic code evolved to maximize this advantage.(2–14) Stereochemical interaction. Stereochemical interactions betw ...
Chapter 24
Chapter 24

... acetyl-ACP and malonyl-ACP by specific transferases. The carboxyl group of malonyl-ACP departs, leaving a carbanion that attacks the acetyl group of acetyl-ACP to produce a four-carbon  -ketoacyl intermediate, which is subsequently reduced by an NADPH-dependent reductase, dehydrated, and reduced a ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
Translation of Cyclin mRNA Is Necessary for Extracts of Activated

... of inducing amphibian oocytes to enter meiosis. It has subsequently been identified in a wide variety of meiotic and mitotic cells from yeast to man (Kishimoto and Kanatani, 1976; Wasserman and Smith, 1978; Sunkara et al., 1979; Kishimoto et al., 1982; Weintraub et al., 1982; Gerhart et al., 1984; M ...
Variation 3.3
Variation 3.3

... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
exam1ans_2007
exam1ans_2007

... D) Y-shaped. Two light chains. Two heavy chains. V-regions at beginning of light and heavy chain are where the antigen binds. The Fab fragment consists of the entire light chain and the V and 1 st constant region of the heavy chain. A diagram was an acceptable answer. ...
Identification
Identification

... P. ramorum may be identified at species level by either its growth characteristics in culture and morphology or by appropriate molecular methods. A flow diagram indicating equivalence or combinations of methods is given in Figure **. Growth characteristics in culture and morphology The growth charac ...
CHAPTER 4  ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox
CHAPTER 4 ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox

... mRNA templates between a defined or identified internal site and unknown sequences at either to the 3' or the 5' -end of the mRNA. It requires two gene sequence-specific primers that flank the region of sequence to amplify relatively few target molecules in a complex mixture (Miao et al., 2010). Th ...
interaction analysis of aspirin with selective amino acids
interaction analysis of aspirin with selective amino acids

... Abstract: This study was conducted to assess the compatibility of aspirin with selective amino acids by studying the effect of amino acids on the solubility of aspirin, so that the attention could be paid towards the use of proteinous foods along with aspirin. Two different types of dissolution medi ...
laboratory methods for diagnosis and detection of
laboratory methods for diagnosis and detection of

The nucleotide sequence of Saccharomyces cerevisiae
The nucleotide sequence of Saccharomyces cerevisiae

... telomere shows good homology with the telomeres of several other chromosomes. There are also several other internal chromosomal regions with long-range homology to other chromosomes. The largest of these is an area common to chromosomes IX and XIV, occurring at 89,233–186,363 and 478,568–616,076, re ...
Block 1 Unit #3
Block 1 Unit #3

... a. Hexokinase has an  affinity for glucose (it is in most tissues) b. Glucokinase in liver (duringgluckose state) 7. Name the regulatory enzymes in glycolysis a. Hexokinase, Phosphofructokinase, pyruvate kinase 8. What role does dihydroxyacetone phosphate play in a) glycolysis b) triglyceride synt ...
A rapid and specific PCR–ELISA for detecting Salmonella typhi
A rapid and specific PCR–ELISA for detecting Salmonella typhi

artificial yeast chromosomes
artificial yeast chromosomes

... in an adenine-deficient minimal medium environment, which makes the yeast dependent on the artificial chromosome for adenine, ensuring that the artificial chromosome is not lost before the start of the experiment. During the experiment, the yeast are grown on YPD, which has plentiful adenine; the ye ...
Structural Prediction of Membrane
Structural Prediction of Membrane

... [l, 21. With their advent have come many secondary-structure prediction methods which require only a knowledge of the amino acid sequence (cf. 13 - 51). These techniques generally rely on a statistical or informational analysis of the frequency with which the 20 amino acids appear within the observe ...
DNA walker circuits - PRISM Model Checker
DNA walker circuits - PRISM Model Checker

Novel Research Starts with GAPDH - Bio-Rad
Novel Research Starts with GAPDH - Bio-Rad

... Forward primer ...
tRNA aminoacylation by arginyltRNA synthetase: induced
tRNA aminoacylation by arginyltRNA synthetase: induced

... recognized mainly by main chain atoms of the protein belonging to the loop between helices H22 and H23 (Figure 2C). This is quite unusual for a strong determinant. This is reinforced by a stacking interaction, where Trp569 is intercalated between Ino34 and Cyt35. Several amino acids, e.g. Tyr491, Ar ...
Molecular mechanics of the interactions of spermine with DNA: DNA
Molecular mechanics of the interactions of spermine with DNA: DNA

... of conformations, however, and there are a variety of distances and positions between the primary and secondary amino groups. Energetics of Spermine/DNA Complexes. Numerical results for spemune/DNA complexes are summarized in Tables I-IV. Because energies for each DNA sequence will be different, abs ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... The PCR-amplified genes of the isolates were subjected to further characterization by restriction enzyme digestion (Table 1). The predicted restriction sites for the gene of B. cereus DSM 3101T were confirmed. The specific restriction sites for EcoRI, HindIII, and PstI were present in most of the ba ...
36. Amino Acids and Carbohydrates in Sediments and Interstitial
36. Amino Acids and Carbohydrates in Sediments and Interstitial

... 70% of the total nitrogen in surface sediments is accounted for by amino acids (Henrichs et al., 1984). A consensus is that these compounds and other labile constituents of seston, e.g., carbohydrates, are remineralized rapidly during epigenetic and diagenetic bacterial activity and that only less t ...
sheet#30
sheet#30

... co2 that comes from bicarbonate by the enzyme carbamoyl phosphate synthetase 1 (CPS1) in the mitochondria. Secondly, the resulting active carbonate links to ammonia. The formed compound is carbamoyl phosphate. This reaction requires two ATP molecules (one for activation and one for linking). CPS1 is ...
Application for rDNA Review/Registration for
Application for rDNA Review/Registration for

... RECOMBINANT DNA Please identify the type of experiment described in this application/registration form by checking the appropriate box(s) below. ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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