Complete Elimination of Endosymbiotic Algae from Paramecium
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
biotransformation - University of California, Berkeley
... The developments of the industrial revolution stimulated a rise in many occupational diseases. Percival Pott in 1775 recognized the role of soot in scrotal cancer among chimney sweeps and the problem was solved by instructing chimney sweeps to clean themselves after work. The causative agents were p ...
... The developments of the industrial revolution stimulated a rise in many occupational diseases. Percival Pott in 1775 recognized the role of soot in scrotal cancer among chimney sweeps and the problem was solved by instructing chimney sweeps to clean themselves after work. The causative agents were p ...
1 Protonolysis of Fe-C bonds of a Diiminopyridineiron(II) Dialkyl
... 4.2. General procedure for the reaction of 1 with protic acids in stoichiometric ratio 1:1. A solution containing approx. 440 mg of compound 1 (0.7 mmol) in 40 ml of THF was stirred at -80 ºC, and a second solution containing exactly the equimolar amount of the corresponding acid (HY = perfluorophen ...
... 4.2. General procedure for the reaction of 1 with protic acids in stoichiometric ratio 1:1. A solution containing approx. 440 mg of compound 1 (0.7 mmol) in 40 ml of THF was stirred at -80 ºC, and a second solution containing exactly the equimolar amount of the corresponding acid (HY = perfluorophen ...
Chapter - I 1 1.1. Introduction to amino acids
... 1.1.1. Overview of the physical properties of the essential amino acids Amino acids, as the building blocks of the most diverse biological compounds, have a characteristic structure. With the exception of proline, all twenty amino acids have an amino group and a carboxyl group with a functional grou ...
... 1.1.1. Overview of the physical properties of the essential amino acids Amino acids, as the building blocks of the most diverse biological compounds, have a characteristic structure. With the exception of proline, all twenty amino acids have an amino group and a carboxyl group with a functional grou ...
The Advanced Placement Examination in Chemistry Acid–Base
... A comparison of the theories Arrhenius, Brønsted and Lewis shows a progressive generalization of the acid base concept. Outline the essential ideas in each of these theories and select three reactions, one that can be interpreted by all three theories, one that can be interpreted by two of them, and ...
... A comparison of the theories Arrhenius, Brønsted and Lewis shows a progressive generalization of the acid base concept. Outline the essential ideas in each of these theories and select three reactions, one that can be interpreted by all three theories, one that can be interpreted by two of them, and ...
2.3.3 Protein and amino acid metabolism
... transporters. Nevertheless, it is apparent that the combination of a multitude of transporters with overlapping specificity and differing energetics, as well as facilitation of both uptake and exchange, makes the physiological interpretation of the roles of these transporters challenging. These amin ...
... transporters. Nevertheless, it is apparent that the combination of a multitude of transporters with overlapping specificity and differing energetics, as well as facilitation of both uptake and exchange, makes the physiological interpretation of the roles of these transporters challenging. These amin ...
the function and synthesis of ribosomes
... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
17 - Wiley
... (c) HClO2 is stronger. O atoms are highly electronegative and attract electron density around them. Having two O atoms, HClO2 will have less electron density in the H–O bond than HClO, and thus making the bond easier to break. 14.47 Use arrows of different sizes to show differences in electron densi ...
... (c) HClO2 is stronger. O atoms are highly electronegative and attract electron density around them. Having two O atoms, HClO2 will have less electron density in the H–O bond than HClO, and thus making the bond easier to break. 14.47 Use arrows of different sizes to show differences in electron densi ...
“The function and synthesis of ribosomes.” Nature Reviews Mol Cell
... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
... of translation, and a key function of the small subunit is to discriminate against aminoacyl-tRNAs that do not match the codon on the message48. This crucial step in the decoding process was poorly understood until the demonstration that the conformation of several residues on the 16S rRNA is sensit ...
Identifying Unknown Bacteria Using Biochemical
... cellular respiration. This lab was tailored for second semester freshmen who are in their first semester of a three-semester introductory biology course. The first semester focuses on molecular biology, bacterial genetics, and introductory biochemistry. This lab was designed for 500 students split i ...
... cellular respiration. This lab was tailored for second semester freshmen who are in their first semester of a three-semester introductory biology course. The first semester focuses on molecular biology, bacterial genetics, and introductory biochemistry. This lab was designed for 500 students split i ...
Gentile, Margaret: Computational Methods for the Design of PCR Primers for the Amplification of functional Markers from Environmental Samples
... designed in a highly conserved segment of amino acids as short as 3 residues. Then primers will be designed with all of the possible combinations of codons which code for these amino acids. Creating the possible primers for only 3 residues yields a much smaller pool than if creating degenerate prime ...
... designed in a highly conserved segment of amino acids as short as 3 residues. Then primers will be designed with all of the possible combinations of codons which code for these amino acids. Creating the possible primers for only 3 residues yields a much smaller pool than if creating degenerate prime ...
Chemistry written examination 1 2008–2012-specifications
... Much research is taking place in Australia into the field of Proteomics. Proteomics is the large scale study of the proteins present in a living organism. The DNA of a cell provides the blueprint for the assembly of the primary structure of proteins, the large biomolecules essential to life. In huma ...
... Much research is taking place in Australia into the field of Proteomics. Proteomics is the large scale study of the proteins present in a living organism. The DNA of a cell provides the blueprint for the assembly of the primary structure of proteins, the large biomolecules essential to life. In huma ...
Chapter 24 Amino Acids, Peptides, and Proteins
... • Conjugated: Bonded to a nonprotein group, such as sugar, nucleic acid, or lipid • Fibrous: Long, stringy filaments, insoluble in water; function as structure • Globular: Folded into spherical shape; function as enzymes, hormones, or transport proteins ...
... • Conjugated: Bonded to a nonprotein group, such as sugar, nucleic acid, or lipid • Fibrous: Long, stringy filaments, insoluble in water; function as structure • Globular: Folded into spherical shape; function as enzymes, hormones, or transport proteins ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... sequences suggested that 6 OTUs were related to the metaloxidizing species of α-, β-, and γ-Proteobacteria (Fig. 1). Possibly chemolithoautotrophic, ε-Proteobacteria were reported to be the dominant species at the northern Loihi Pele’s vents (Moyer et al., 1995). The phylogenetic diversity of metal- ...
... sequences suggested that 6 OTUs were related to the metaloxidizing species of α-, β-, and γ-Proteobacteria (Fig. 1). Possibly chemolithoautotrophic, ε-Proteobacteria were reported to be the dominant species at the northern Loihi Pele’s vents (Moyer et al., 1995). The phylogenetic diversity of metal- ...
Nutrigenomics: Food to match your genes
... A screening test indicates the level of risk that is present for a condition such as a neural tube defect. A positive result from screening suggests that there is an increased chance of a particular condition being present and a negative result means there is a decreased risk. P ...
... A screening test indicates the level of risk that is present for a condition such as a neural tube defect. A positive result from screening suggests that there is an increased chance of a particular condition being present and a negative result means there is a decreased risk. P ...
E. Diuretics
... 1) Major differences between the two series of 5sulfamoyl-benzoic acids in the nature of the functional groups that can be substituted into the 2-and 3positions with the retention of maximal diuretic activity: i) Substituent that can be tolerated on the 2-amino group of the 5-sulfamoyl-2-aminobenzoi ...
... 1) Major differences between the two series of 5sulfamoyl-benzoic acids in the nature of the functional groups that can be substituted into the 2-and 3positions with the retention of maximal diuretic activity: i) Substituent that can be tolerated on the 2-amino group of the 5-sulfamoyl-2-aminobenzoi ...
Lesson Overview - Dr. Thornton`s Courses
... Many mutations are harmful to the organism, but breeders can often produce a few mutants—individuals with mutations—with useful characteristics that are not found in the original population. For example, scientists have developed hundreds of useful mutant bacterial strains by treating bacteria with ...
... Many mutations are harmful to the organism, but breeders can often produce a few mutants—individuals with mutations—with useful characteristics that are not found in the original population. For example, scientists have developed hundreds of useful mutant bacterial strains by treating bacteria with ...
A Major Species of Mouse μ-opioid Receptor mRNA and Its
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
Choosing the Right Centrifuge for Your - Laboratory
... A centrifuge is a critical piece of equipment for the laboratory, and often a significant investment. Therefore, when the time comes to add a new centrifuge—or replace an old one—it is important to make an informed decision. There is a good chance that your needs have changed since the last time you ...
... A centrifuge is a critical piece of equipment for the laboratory, and often a significant investment. Therefore, when the time comes to add a new centrifuge—or replace an old one—it is important to make an informed decision. There is a good chance that your needs have changed since the last time you ...
Molecular characterization of dioxygenases from polycyclic aromatic
... degraders, were positive in both tests. From the three positive strains, complete open reading frames of the nidA and nidB genes were amplified by PCR, using primers designed according to the known nidA and nidB sequences from PYR-1, cloned in the pBAD/ThioTOPO vector and sequenced. The sequences sh ...
... degraders, were positive in both tests. From the three positive strains, complete open reading frames of the nidA and nidB genes were amplified by PCR, using primers designed according to the known nidA and nidB sequences from PYR-1, cloned in the pBAD/ThioTOPO vector and sequenced. The sequences sh ...
Biology OF CLONING VECTORS-III-B.SC - E
... 1. write in short notes about the purification of plasmid DNA? The purification of plasmid DNA An obvious prerequisite for cloning in plasmids is the purification of the plasmid DNA. Although a wide range of plasmid DNAs are now routinely purified, the methods used are not without their problems. Un ...
... 1. write in short notes about the purification of plasmid DNA? The purification of plasmid DNA An obvious prerequisite for cloning in plasmids is the purification of the plasmid DNA. Although a wide range of plasmid DNAs are now routinely purified, the methods used are not without their problems. Un ...
biology - Kendriya Vidyalaya No.1 Kanchrapara
... SET-II (Repeated 1-2 times) 1. What is meant by withdrawal symptoms ? 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types ...
... SET-II (Repeated 1-2 times) 1. What is meant by withdrawal symptoms ? 2. Give scientific name of two helminthes that are pathogene to man . 3. What is incubatiuon period ? how long it is for AIDS virus ? 4. Name and explain the two types of immune responses in humans . 5. Name the two special types ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.