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Genes: Definition and Structure
Genes: Definition and Structure

... ribosomes, transfer RNAs (tRNAs), and a variety of protein enzymes and ‘factors’ – uses the mRNA template to direct the synthesis of a protein, a process called translation. The DNA of the chromosome contains many genes lined up one after another, but mRNAs generally contain the message for only one ...
A Rapid Method for the Identification of Plasmid Desoxyribonucleic
A Rapid Method for the Identification of Plasmid Desoxyribonucleic

... Currently two types of rapid screening techniques for plasmid desoxyribonucleic acid (DNA) are used (1,4,5,7). One type requires little starting material, but subjects the DNA to considerable stress during lysis (5,7) or during separation of plasmid DNA from chromosomal DNA (1) and is therefore not ...
Assignment CHE-09 TMA-01,02 Year 2005
Assignment CHE-09 TMA-01,02 Year 2005

The diagram below shows a partial sequence of nucleotide bases
The diagram below shows a partial sequence of nucleotide bases

Temporal control of Transcription in phage SPO
Temporal control of Transcription in phage SPO

... •  When hfl is absent or mutated lysogeny is highly favored as this protease cleaves cIII •  When cells are starved and not dividing protease levels are low and cII and cIII levels are stabilized thus favoring ...
RNAi minilecture and Using Forward Genetics to Explore Complex
RNAi minilecture and Using Forward Genetics to Explore Complex

... • In other cases dsRNA acts as an intermediate, for example when 'aberrant' mRNAs are copied by cellular RdRP. • Transcription can produce dsRNA by readthrough from adjacent transcripts, as may occur for repetitive gene families or high-copy arrays (blue dashed arrows). • Alternatively, transcriptio ...
Carbon-Based Molecules
Carbon-Based Molecules

... hydrogen, and oxygen, and they include sugars and starches. Carbohydrates can be broken down to provide a source of usable chemical energy for cells. Carbohydrates are also a major part of plant cell structure. The most basic carbohydrates are simple sugars, or monosaccharides ...
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VGEC: Student Notes RESTRICTION ENZYME MAPPING OF THE λ
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... Restriction endonucleases are powerful tools for the molecular analysis of complex genomes such as those of mammals. These enzymes can be isolated from a wide variety of micro-organisms and have the property of cutting both strands of double-stranded DNA only at a specific nucleotide sequence, usual ...
Transcription and Translation
Transcription and Translation

...  3 nucleotides of mRNA = codon = amino acid  The “interpreter” tRNA delivers the proper complimentary base to the ribosome. Anticodons are blocks of 3 tDNA bases that actually attach to the correct protein.  The anticodon( tRNA) binds by complimentary base pairing to the nucleotides of the codon. ...
Methods of asexual reproduction
Methods of asexual reproduction

... that are genetically identical to the parent organism. • Sexual reproduction results in offspring that are genetically different from the parent organisms. ...
APPLICATION OF mtDNA TECHNOLOGY IN FORENSIC SCIENCE
APPLICATION OF mtDNA TECHNOLOGY IN FORENSIC SCIENCE

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Click to add title - Tech Transfer Central
Click to add title - Tech Transfer Central

... the examiner's product-of-nature rejection is still an issue in this case, in the interest of judicial economy we rule on it now . . . We hold that Bergy's claim 5 clearly does not define a product of nature. ...
Bio 392: Study Guide for Final
Bio 392: Study Guide for Final

... o Identify the three parts of a nucleotide o Identify which bases are pyrimidines and which bases are purines o Know the following individuals and their contributions to the discovery of DNA as genetic material: Erwin Chargaff, Rosalind Franklin, James Watson, and Francis Crick o Describe how DNA co ...
Western blot analysis
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... (NC009456.1 and NC009457.1) and Yersinia pestis Angola (NC010159.1) from JCVI and the Shigella flexneri 2a str. 301 (NC004337.2) genome sequences from NCBI genome using the GenBank FTP site. We considered 5 amino acid residues upstream and downstream of the 512 unique phosphotyrosine sites identifie ...
Electrophoretic stretching of DNA molecules using microscale T
Electrophoretic stretching of DNA molecules using microscale T

... trapping and stretching of DNA molecules up to lengths of 485 kbp are demonstrated. © 2007 American Institute of Physics. 关DOI: 10.1063/1.2745650兴 The ability to trap and stretch biopolymers is important for a number of applications ranging from single molecule DNA mapping1 to fundamental studies of ...
Pierce Genetics Testbank questions: Chapter 1
Pierce Genetics Testbank questions: Chapter 1

... Blending inheritance – The idea that the genetic material is a fluid that gets blended during sexual reproduction between a male and female, resulting in the production of traits in the offspring that are blended intermediates of those of the parents. (b) Why do you think this particular idea was wi ...
Direct measurement of electrical transport through DNA molecules
Direct measurement of electrical transport through DNA molecules

... states7,8, which could, for example, be associated with the base pairs. The hopping process could be either unidirectional or involve one-dimensional diffusion. It can be argued that the back-and-forth diffusive hopping8 is less likely in our case due to the high electric ®elds used, which will tilt ...
Genomic DNA Extraction Kit INSTRUCTION MANUAL
Genomic DNA Extraction Kit INSTRUCTION MANUAL

Chapter 25 RNA Metabolism
Chapter 25 RNA Metabolism

...  But Berget and Sharp (1977) observed singlestranded DNA loops when examining adenovirus mRNA-DNA hybrids by electron microscopy.  Such single-stranded DNA loops was widely observed when examining such RNA-DNA hybrids.  Intron sequences were proposed to be present on the template DNA sequences, w ...
PP 7.2
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... secretions [8, 12]. Therefore further studies are needed that incorporate next-generation sequencing to find more reliable markers that can differentiate between saliva and vaginal secretion sample. ...
lecture 20
lecture 20

... way of changing and recording the change in its genome RNA evolved faster replication and a shorter genome In all the experiments, investigators had to add enzymes for ribozymes to replicate themselves ...
Genomic DNA & cDNA Libraries
Genomic DNA & cDNA Libraries

... to be converted to the double strand cDNA. The mRNA cDNA complex is treated with an alkali which hydrolyzes the mRNA, but not the cDNA. Then by using terminal transferase which is a DNA polymerase that adds deoxynucleotides to free 3 ends without the need of template (this will add poly G). To this ...
Section 20.1
Section 20.1

... • Vectors are carrier DNA molecules that can replicate cloned DNA fragments in a host cell • Vectors must be able to replicate independently and should have several restriction enzyme sites to allow insertion of a DNA fragment • Vectors should carry a selectable gene marker to distinguish host cells ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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