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Practice Final Exam - mvhs
Practice Final Exam - mvhs

... stp90 gene-- This codes for the stp90 protein what binds to the operator region in front of the HLN gene to prevent transcription (when activated). The stp90 protein becomes activated(to bind to operator) when there is mannose (a sugar) present. You can assume that the E. coli you use do not have an ...
Presentation
Presentation

... • 11.3 How Is DNA Replicated? • 11.4 How Are Errors in DNA Repaired? • 11.5 What Are Some Applications of Our Knowledge of DNA Structure and ...
Chapter 7C
Chapter 7C

... The flexibility of activatormediated gene transcription in eukaryotes is exploited in the widely used yeast two-hybrid system, which is an in vivo system for identification of interacting pairs of proteins. In this method, genes encoding hybrid proteins containing yeast DNA-binding and activation do ...
BIOL 1107 - Chapter 17
BIOL 1107 - Chapter 17

Biotechnology
Biotechnology

... potential allergic reactions. Most insulins today are chemically identical to natural human insulin. ...
Effect of Novobiocin and Other DNA Gyrase lnhibitors on Virus
Effect of Novobiocin and Other DNA Gyrase lnhibitors on Virus

... labelled in the presence of 5 x 10 -4 M it is of interest to note that the absolute amount of 'nov-resistant' synthesis in uninfected cells corresponds closely to that seen for cellular D N A in infected cells in the presence or absence of nov. For all four antibiotics tested, the concentration requ ...
1 - u.arizona.edu
1 - u.arizona.edu

... - adenine and guanine nucleotides both inhibit the synthesis of IMP early in pathway at both PRPP synthetase and PRPP amidotransferase; they also feedback inhibit their own product from IMP - excess ATP activates pathway from IMP  GMP, while excess GTP activates pathway from IMP  AMP to maintain p ...
Chapter 12
Chapter 12

... – DNA is isolated from biological fluids left at a crime scene – The technique determines with near certainty whether two samples of DNA are from the same individual • DNA technology—methods for studying and manipulating genetic material—plays significant roles in many areas of society Copyright © 2 ...
DNA sequencing
DNA sequencing

... variation in which each possible sequence is present in at least 1% of people. For example, a place in the genome where 93 percent of people have a T and the remaining 7 percent have an A is a polymorphism. If one of the possible sequences is present in less than 1 percent of people (99.9 percent of ...
official course outline information
official course outline information

... Students are advised to keep course outlines in personal files for future use. Shaded headings are subject to change at the discretion of the department and the material will vary - see course syllabus available from instructor FACULTY/DEPARTMENT: BIO 403 COURSE NAME/NUMBER ...
Question 2: Multiple-Choice Standard: Chemistry of Life
Question 2: Multiple-Choice Standard: Chemistry of Life

... Which of the following best describes the molecular structure of ovalbumin? A. a group of six carbon atoms joined in a ring B. a chain of amino acids folded and twisted into a molecule C. a set of three fatty acids attached to a molecule of glycerol D. a sequence of nitrogenous bases attached to a s ...
Mechanisms of Enzymes
Mechanisms of Enzymes

... – His 12 (deprotonated) accepts the H of 2’OH, Followed by a nucleophilic attack by 2’ O on P –Simultaneously - His 119 (protonated) donates H+ to other side of phosphate bond. –Lysine stabilizes (-) of phosphate –When His 12 and 119 are done cyclic O-P-O is formed –After cleavage and departure of R ...
No Slide Title
No Slide Title

... contain telomeres so that don’t lose ends contain a selectable marker (usually a gene for amino acid or nucleoside ...
pGLO Transformation SV
pGLO Transformation SV

... In this lab, you will be using non-pathogenic E. coli bacteria and pGLO, a plasmid modified with two genes. The pGLO plasmid contains the genetic codes for (see Table 2): 1. a green fluorescent protein (GFP) from the bioluminescent jellyfish, Aequorea victoria 2. ampicillin resistance (amp) 3. a spe ...
Tutorial Kit (Biochemistry-300 L)
Tutorial Kit (Biochemistry-300 L)

... 1. What are enzymes? What is the importance of enzymes for living beings? Enzymes are proteins that are catalysts of chemical reactions. Enzymes are catalysts that are non-consumable substances that reduce the activation energy necessary for a chemical reaction to occur. Enzymes are highly specific ...
Strain Improvement - Bharathiar University
Strain Improvement - Bharathiar University

... For example, one of the codons in DNA coding for the amino acid Alanine is CGG. If the middle base is changed by SDM from G to C the codon sequence becomes CCG which codes for a different amino acid (Glycine). ...
Plasmid
Plasmid

... Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. ...
Proteins - Sewanhaka Central High School District
Proteins - Sewanhaka Central High School District

... Nucleic acids are built from nucleotides • Nucleotides – Phosphate group – 5 carbon sugar – Nitrogen base ...
Creation/Evolution - Geoscience Research Institute
Creation/Evolution - Geoscience Research Institute

... run antiparallel, palindromes are defined as any double-stranded DNA in which reading 5’ to 3’ both are the same Some examples: The EcoRI cutting site: – 5'-GAATTC-3' – 3'-CTTAAG-5' ...
Genome Sequencing Using a Mapping Approach
Genome Sequencing Using a Mapping Approach

... Shotgun Approach 1. The shotgun approach obtains a genomic sequence by breaking the genome into overlapping fragments for cloning and sequencing. 2. A computer is then used to assemble the genomic sequence. 3. Advances that have made this approach practical for large genomes include: a. Better compu ...
DNA Content of Nuclei andChromosome
DNA Content of Nuclei andChromosome

... would have favored those cells seldom giving rise to nondividing progeny. These would, therefore, have become the new stem-line cells. Thus, the production of nondividing cells may be regarded as an indication of the instability of the 2«tumor subline, these cells being selected against in the pres ...
Thank-you for attending Biol120 Mock Final Exam, brought to you by
Thank-you for attending Biol120 Mock Final Exam, brought to you by

... a) DNA uses cytosine, guanine, uracil, and adenine; whereas RNA uses cytosine, thymine, guanine, and adenine b) DNA has no free hydroxyl group on the 2’ carbon and RNA has a free hydroxyl group on the 2’ carbon c) DNA is usually single-stranded and RNA is usually double-stranded d) None of the above ...
Chapter 8
Chapter 8

... These are then used in PCR to generate random mutations in our gene of interest that has been cloned into a plasmid.  The left and right portions of the target DNA are amplified separately by PCR.  The amplified fragments are amplified, denatured to make them single stranded, and then re-annealed. ...
View Essential-4 Data Sheet
View Essential-4 Data Sheet

... beneficial intestinal micro-organisms for nutritional health and well-being are known as probiotics. Probiotic dietary lactobacilli colonize the intestinal tract, depending on the specific bacterium-to-host affinity. Bacteria are continually in competition for colonization space. The original bacter ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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