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Curriculum and Training Specialist Bio
Curriculum and Training Specialist Bio

... 4. Crime Scene Investigators search in areas of the genome that are unique from individual to individual and are “anonymous” (control no known trait or function) The areas examined are Short Tandem Repeats or STR’s ...
Gel Electrophoresis - Integrated DNA Technologies
Gel Electrophoresis - Integrated DNA Technologies

... can be thought of as semi-solid matrices whose pore sizes aid in separation. The semisolid nature of the gel participates through a process known as molecular sieving. The three common media for gel electrophoresis are starch, polyacrylamide, and agarose. Of these, the starch gel medium is the leas ...
Protocol for QuickExtract™ RNA Extraction Kit
Protocol for QuickExtract™ RNA Extraction Kit

FREE Sample Here
FREE Sample Here

... the genetic material of cells and viruses. These differ primarily in the structure of their monomers, which are called nucleotides. Each nucleotide consists of phosphate, a pentose sugar (deoxyribose or ribose), and one of five cyclic nitrogenous bases: adenine (A), guanine (G), cytosine (C), thymin ...
Week 5 - Reverse Transcriptase Polymerase Chain Reaction
Week 5 - Reverse Transcriptase Polymerase Chain Reaction

... same region are made, it stands out against the total genetic material of an organism. This allows specific genes and other regions to be studied in detail. RT-PCR (Reverse Transcriptase PCR) starts with total mRNA rather than DNA. This allows one to amplify and clone an expressed region of the geno ...
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... Creation scientist. Jack Szostak is working to recreate a recipe that transformed chemistry into biology. ...
The Blueprint of Life, From DNA to Protein
The Blueprint of Life, From DNA to Protein

... Review of DNA basics •Composed of deoxyribonucleotides Covalently bonded in chains 5’ end (phosphate) 3’ end (hydroxyl) •Double-stranded •Strands are anti-parallel •Strands are complementary ...
CRACKING THE GENETIC CODE
CRACKING THE GENETIC CODE

... amino acids, beginning protein synthesis. The nascent protein chain is elongated by the subsequent binding of additional tRNAs and formation of a peptide bond between the incoming amino acid and the end of the growing chain. Although this general process was understood, the question remained: How do ...
Unit II Chemistry
Unit II Chemistry

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... c) Chromosomes float freely in the cytosol. d) Eukaryotic cells contain no genetic material outside of their chromosomes. ...
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Biotechnology and Genetic Engineering

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Lecture Slides

... • Nitrogenous bases hang off the sugar-phosphate backbone. ...
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Lab #1: Alu Lab, Part 1

... With our DNA successfully isolated from our cheek cells, we’re ready to begin the PCR. We’ll set-up this reaction today and allow it to run overnight. We’ll analyze our samples using gel electrophoresis during our next lab session. A). Set up group controls: Each lab group will need to obtain and la ...
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220 08Summary13 - Earth and Atmospheric Sciences

... molecule that could do it all - assemble a copy of itself (growth, reproduction) and provide the energy to do so (metabolism). One macromolecule, RNA (ribonucleic acid) is the prime suspect because it can Store, transmit and duplicate genetic information (like DNA) Catalyze chemical reactions (unlik ...
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trans trans review game[1]

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A novel environment-sensitive biodegradable polydisulfide with

... was comparable to or slightly lower than that of corresponding PEI complexes. Moreover, the polydisulfide showed better serum-friendly feature than PEI when delivering either DNA or siRNA in the presence of 10% FBS. This novel polydisulfide is a promising lead for further design and development of s ...
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SBARS: fast creation of dotplots for DNA sequences on different

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Section 13-1 Ghanging the Living World

... A breeder breeds rabbits 2 and 8. What trait is the breeder most likely interested in? black ...
Ex2_09dev - Columbia University
Ex2_09dev - Columbia University

... stranded ends. The 4 molecules are drawn on the last page. (In all cases W is the top strand). You have a DNA polymerase with no exonuclease activity, and you have radioactive dXTPs. (Radioactivity is in the bases). A. You want to make a DNA molecule (in vitro) that has radioactivity in Watson, but ...
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... FISH fluorescent in situ hybridization: (FISH) A technique used to identify the presence of specific chromosomes or chromosomal regions through hybridization (attachment) of fluorescently-labeled DNA probes to denatured chromosomal DNA. Step 1. Preparation of probe. A probe is a fluorescently-label ...
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BMB 400 PART THREE
BMB 400 PART THREE

... "language" of nucleic acids to that of proteins is the genetic code. Experiments testing the effects of frameshift mutations showed that the deletion or addition of 1 or 2 nucleotides caused a loss of function, whereas deletion or addition of 3 nucleotides allowed retention of considerable function. ...
The Body`s Fundamental Building Blocks
The Body`s Fundamental Building Blocks

... The Body’s Fundamental Building Blocks What are amino acids and why are they important? Known as the “building blocks” of proteins, amino acids have many important functions in the body including the regulation of muscle and hormone activity and the formation and maintenance of every tissue in the b ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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