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... • Six functional groups are important in the chemistry of life – Hydroxyl – in alcohols, sugar – Carbonyl – in sugars, amino acids, nucleotide bases – Carboxyl – in amino acids, fatty acids; acts as an acid and ...
sample mt exam - Ltcconline.net
sample mt exam - Ltcconline.net

... d. a protein is to an amino acid e. an amino acid is to a nucleic acid ...
Gene Section DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) Atlas of Genetics and Cytogenetics
Gene Section DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) Atlas of Genetics and Cytogenetics

... is a covalent chemical modification, resulting in the addition of a methyl (CH3) group at the carbon 5 position of the cytosine ring. DNMT3B uses Sadenosyl-L-methionine (AdoMet) as the source of the methyl group being transferred to the DNA bases. The methyl group of S-adenosylomethionine is bound t ...
Proximal promoter
Proximal promoter

... the distal sequence upstream of the gene that may contain additional regulatory elements, often with a weaker influence than the proximal promoter – Anything further upstream (but not an enhancer or other regulatory region whose influence is positional/orientation independent) – Specific transcripti ...
Chapter-12 PTT
Chapter-12 PTT

... Molecular biologists have perfected DNA fingerprinting so that it is possible to use the technique to provide evidence to solve crimes and even identify a child's parents. Recently, a U.S. immigrant asked the U.S. Citizenship and Immigration Services for permission to have her young daughter who was ...
The RNA polymerase factory: a robotic in vitro assembly platform for
The RNA polymerase factory: a robotic in vitro assembly platform for

... models are based on features of individual structures [e.g. separation of the transcript from the DNA template strand (7)], whereas other mechanisms have been proposed by comparing the conformations of protein domains in different crystals or by co-crystallizing polymerases in the presence of various ...
PDF
PDF

... coli ung geneis described. Transcriptioninitiation and from the DNA which can arise as a result of either misincortermination sites were determinedby S1 nuclease and poration of dUMP residues by DNA polymerase or due to RNase mapping. The common prokaryotic -35, -10, deamination of cytosine. None of ...
Protocol for inserting transgene using Tn7 (6/1/06) This system uses
Protocol for inserting transgene using Tn7 (6/1/06) This system uses

... These plasmids are identical, except that pGRG25 does not have the SmaI site. Unique sites in pGRG36 are PacI, SmaI, NotI, XhoI, AvrII and AscI sites in the multiple cloning site. Sequences of both of these plasmids can be found using the GenBank accession # DQ460223. 2. Prepare DNA for transformati ...
The History of Molecular Biology
The History of Molecular Biology

... distinguished physicist's exploration of the question which lies at the heart of biology, it was written for the layman, but proved one of the spurs to the birth of molecular biology and the subsequent discovery of the structure of DNA. The philosopher Karl Popper hailed it as a 'beautiful and impor ...
The Effect of Actidione and other Antifungal Agents on Nucleic Acid
The Effect of Actidione and other Antifungal Agents on Nucleic Acid

... Preliminary investigations. The manometric techniques of Umbreit, Burris & Stauffer (1949) were used to study the effect of actidione on oxygen consumption and anaerobic production of carbon dioxide by Saccharornyces carlsbergensis in the presence of glucose. Actidione at concentrations up to 1 mg./ ...
C:\exams\Aug_04\Biology\final\Biology 3201 August 2004.wpd
C:\exams\Aug_04\Biology\final\Biology 3201 August 2004.wpd

... adenine and guanine adenine and thymine cytosine and thymine cytosine and uracil ...
1. The carbon atoms of cysteine are derived from: A. Methionine B
1. The carbon atoms of cysteine are derived from: A. Methionine B

... Synthesis of the carbohydrate component occurs in both the endoplasmic reticulum and the Golgi. The synthesis of the common oligosaccharide requires dolichol phosphate and takes place associated with the endoplasmic reticulum membrane. The carbohydrate component contains a common oligosaccharide mad ...
Recombinant DNA Techniques - The University of Oklahoma`s
Recombinant DNA Techniques - The University of Oklahoma`s

... 2. Add 7 ml onion paste to a 15 ml tube. Add 7 ml lysis solution and mix gently by inversion. May need to mix paste and solution with spatula before inverting. Mix until the blue lysis solution is evenly dispersed. 3. Place tube in 55-65 degree C waterbath (or water heated to correct temperature in ...
Mrs. Lee – 8th Grade Physical Science Page 1 Chapter 8 Practice
Mrs. Lee – 8th Grade Physical Science Page 1 Chapter 8 Practice

... ______ b (hydroxyl group) 16) A combination of an oxygen atom and a hydrogen atom, -OH, is called a hydrocarbon. a) True b) False ______ b (Proteins) 17) Nucleotides are polymers made of amino acids. a) True b) False ______ b (saturated) 18) A hydrocarbon with only single bonds in its carbon chain i ...
C454_lect10 - University of Wisconsin
C454_lect10 - University of Wisconsin

... Υ-Crystallin (eye lens protein) lasts as long as the organism does. ...
Power point presentation
Power point presentation

... amino acids.  Proteins are important in cell structure, and as enzymes, (which we all know speed up reactions and lower activation energies in the body) Many antibodies, which fight infection, are protein molecules that are ...
7.03 Fall 2003 Problem Set #3 Solutions
7.03 Fall 2003 Problem Set #3 Solutions

... 3'GTT5' template strand 5'CAA3' coding strand (codes for Gln) 3'ACT5' template strand 5'TGA3' coding strand (codes for UGA stop codon) 3'ATC5' template strand 5'TAG3' coding strand (codes for UAG stop codon) Notice that for UGA and UAG, the two candidate sequences for mutation both encode amino acid ...
Chapter Eleven: Chromosome Structure and Transposable Elements
Chapter Eleven: Chromosome Structure and Transposable Elements

Genomic DNA extraction from small amounts of serum to be used for
Genomic DNA extraction from small amounts of serum to be used for

Chapter 17. Application of Recombinant DNA Technology in
Chapter 17. Application of Recombinant DNA Technology in

... manipulation of organisms or their genetic components to make useful products. • To work directly with specific genes, scientists prepare genesized pieces of DNA in identical copies, a process called DNA cloning. ...
GMO Investigator™ Kit - Bio-Rad
GMO Investigator™ Kit - Bio-Rad

Foundations of Biology
Foundations of Biology

... The most highly packaged form of DNA is “heterochromatin” Heterochromatin cannot be transcribed, therefore expression of genes is prevented Chromosome puffs on some insect chomosomes illustrate where active gene expression is going on ©2000 Timothy G. Standish ...
Document
Document

... 4. Crime Scene Investigators search in areas of the genome that are unique from individual to individual and are “anonymous” (control no known trait or function) The areas examined are Short Tandem Repeats or STR’s ...
The DNA repair helicase UvrD is essential for replication
The DNA repair helicase UvrD is essential for replication

... • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA requires ssDNA for nucleation) • Why is the RecG reaction unidirectional? ...
+ H 2 O(l) - Cloudfront.net
+ H 2 O(l) - Cloudfront.net

... • Exists in solution completely or almost completely as ions. • All ionic compounds and a few molecular compounds.(Ex: Strong Acids) HCl (aq) NaCl( s) ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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