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PPT3
PPT3

... What we want is a deterministic algorithm for applying the inter- and intramolecular recombination operations to descramble an arbitrary gene. ...
MNS Blood Group System variants on Malarial Resistance
MNS Blood Group System variants on Malarial Resistance

... pigmented-trophozoite, and schizont stage parasites within the infected RBC. P. falciparum-infected erythrocytes express parasite-derived adhesion molecules on their surface, resulting in sequestration of pigmented-trophozoite and schizont stage-infected RBCs in the microvasculature. The asexual int ...
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slg mock midterm – for practice only

... parental double helix. b. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA. c. The two strands of the parental molecule separate, and each functions as a template for synthesis of a new, complementary strand. d. DNA Polymerase III carries out synthesis by ex ...
Manual: Universal Human miRNA Reference RNA
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... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...


Recombinant DNA and Gene Cloning
Recombinant DNA and Gene Cloning

... Genes on plasmids with high numbers of copies are usually expressed at high levels. In nature, these genes often encode proteins (e.g., enzymes) that protect the bacterium from one or more antibiotics. Plasmids enter the bacterial cell with relative ease. This occurs in nature and may account for th ...
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... acid in the stomach with a mild alkaline substance. Some of the more modern antacids work by stopping the stomach from producing the acid in the first place, often you can work out the difference by reading the description on the box. Those brands offering “immediate relief” are more likely to be ba ...
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Biosynthesis of Plant-derived flavor compounds

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... (16 pts) You are studying a mouse gene named MtoA. You know that the protein product of this gene localizes to mitochondria in mouse cells. Your final goal is that you want to know whether mouse MtoA protein is capable of localizing to mitochondria if produced in yeast cells. You decide to make a pl ...
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Catherine Dong Professor Bert Ely Biology 303H 1 November 2012
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... presumably because they copy their germline DNA more often per year” (Bromham L. 2009). In other words, more copies of germline DNA results in higher mutation rates because the more DNA that is replicated, the higher the chance that the processes will make a mistake (Lynch 2009). This factor explain ...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...

... The plates are incubated seven days at 25°C in a plastic bag to prevent drying. Pairings giving wild type growth are repeated on minimal agar plates (C orrell et al. 1987) to confirm the complementation reaction. Complementation reactions are more definitive when the auxotrophic mutants are separate ...
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Endosymbiosis: The Evolution of Metabolism
Endosymbiosis: The Evolution of Metabolism

... Why is oxidative phosphorlylation (most of ATP synthesis) done in the mitochondria? Why is the light reaction of photosynthesis done in chloroplasts? Chloroplasts and mitochondria are peculiar organelles. They have double membranes and their own circular DNA molecules. They also reproduce independen ...
GLP 019 - University of Newcastle
GLP 019 - University of Newcastle

Solutions to 7
Solutions to 7

... i) variant 2 will bind Minoxidil but variant 3 will not bind Minoxidil. In variant 2, one of the 2 hydrogen bonds remains, as does the hydrophobic pocket, and given the information this is enough to allow binding. In variant 3, both hydrogen bonds have been lost, and this disrupts the binding. ii) v ...
PCR Applications
PCR Applications

... 3) Without looking at your actual gel results, consider the genotypes TT, Tt, and tt. Hypothesize or predict how many bands you will see after Hae III digestion for each genotype if we assume that Hae III only cuts the taster allele once, but does not cut the non-taster allele at all. Please keep in ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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