Optical Tweezers: Measuring Piconewton Forces
... objective is always equal to or greater than the input aperture of the objective. Thus, light that is deflected by the trapped bead is not collected by the objective. However, a dual-beam instrument can sacrifice some trapping force and only fill half of the objective input aperture, as shown in Fig ...
... objective is always equal to or greater than the input aperture of the objective. Thus, light that is deflected by the trapped bead is not collected by the objective. However, a dual-beam instrument can sacrifice some trapping force and only fill half of the objective input aperture, as shown in Fig ...
Biochemistry 462a - Proteins Extra Questions
... 5. The following question deals with the properties of amino acid sidechains buried in the hydrophobic interior of a protein. (A) Would the pKa of a buried lysine be higher or lower than the pKa of a surface Lys? (B) Would the strength of a buried hydrogen bond be stronger or weaker than a hydrogen ...
... 5. The following question deals with the properties of amino acid sidechains buried in the hydrophobic interior of a protein. (A) Would the pKa of a buried lysine be higher or lower than the pKa of a surface Lys? (B) Would the strength of a buried hydrogen bond be stronger or weaker than a hydrogen ...
Bioknowlodgy worksheet 2.4
... Draw and annotate a structural diagram below to outline how two generalised amino acids (i.e. use the R-group nomenclature) into a dipeptide through condensation, producing a peptide bond. ...
... Draw and annotate a structural diagram below to outline how two generalised amino acids (i.e. use the R-group nomenclature) into a dipeptide through condensation, producing a peptide bond. ...
NOTE Corynebacterium efficiens sp. nov., a glutamic
... 1n0 g l−") when they were cultured aerobically at 45 mC and provided with 6 % glucose as a carbon source. The isolates grew well between pH 7 and 10, but not at pH 6n5 or below. They showed good growth in the presence of 10 % NaCl. YS-155 grew slightly in the presence of 15 % glucose, but YS-314T an ...
... 1n0 g l−") when they were cultured aerobically at 45 mC and provided with 6 % glucose as a carbon source. The isolates grew well between pH 7 and 10, but not at pH 6n5 or below. They showed good growth in the presence of 10 % NaCl. YS-155 grew slightly in the presence of 15 % glucose, but YS-314T an ...
DNA cloning by homologous recombination in Escherichia coli
... ence of either RecE/RecT or oligonucleotides containing the homology arms at their 5′ end, and PCR primers at their 3′ ends for Redα/Redβ, and the absence of amplification of the plasmid origin/antibiotic resistance gene cassettes. (B). Summary of five examples of the reaction diagrammed in (A). The ...
... ence of either RecE/RecT or oligonucleotides containing the homology arms at their 5′ end, and PCR primers at their 3′ ends for Redα/Redβ, and the absence of amplification of the plasmid origin/antibiotic resistance gene cassettes. (B). Summary of five examples of the reaction diagrammed in (A). The ...
Understanding its origins and mechanism of action
... enzyme cyclooxygenase (COX). COX is available in two isoforms, COX-1 and COX-2. Aspirin inhibits both the enzymes cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX2). By adding an acetyl group (−C(O)CH3) to an amino acid residue, the COX enzymes lose their ability to transform arachidonic acid into ...
... enzyme cyclooxygenase (COX). COX is available in two isoforms, COX-1 and COX-2. Aspirin inhibits both the enzymes cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX2). By adding an acetyl group (−C(O)CH3) to an amino acid residue, the COX enzymes lose their ability to transform arachidonic acid into ...
Topic 4: BIOLOGICALLY IMPORTANT ORGANIC MOLECULES
... in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of the molecule is very polar and hydrophilic while the other part of the molecule is non- ...
... in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of the molecule is very polar and hydrophilic while the other part of the molecule is non- ...
Lesson One Plans
... Remember that the four basic biological molecules that make up cells are nucleic acids, proteins, carbohydrates, and lipids. We can actually separate these molecules using regular household products. Our task for today is to extract DNA from the nucleus of wheat germ cells. Sounds tricky, but in fac ...
... Remember that the four basic biological molecules that make up cells are nucleic acids, proteins, carbohydrates, and lipids. We can actually separate these molecules using regular household products. Our task for today is to extract DNA from the nucleus of wheat germ cells. Sounds tricky, but in fac ...
Topic 4 - FSU Biology
... in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of the molecule is very polar and hydrophilic while the other part of the molecule is non- ...
... in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of the molecule is very polar and hydrophilic while the other part of the molecule is non- ...
Unit 2: Introduction to Biology – Molecules of Life – Part 1
... are HUGE molecules made up of molecules called nucleotides. Below is a picture of a nucleotide. The nucleic acids are found in the nucleus of eukaryotic cells. DNA never leaves the nucleus because it is protected from chemicals that would damage or destroy it inside of this important cell structure. ...
... are HUGE molecules made up of molecules called nucleotides. Below is a picture of a nucleotide. The nucleic acids are found in the nucleus of eukaryotic cells. DNA never leaves the nucleus because it is protected from chemicals that would damage or destroy it inside of this important cell structure. ...
Benfotiamine 150 + Alpha-Lipoic Acid 300
... Advanced Glycation End Products (AGEs). AGEs are formed by a complex series of reactions between reducing sugars and amino acids on proteins, lipids, and nucleic acids. Diet is a major external source of AGEs, with about 10% of AGEs from food remaining in the body after ingestion 20; bodily AGE leve ...
... Advanced Glycation End Products (AGEs). AGEs are formed by a complex series of reactions between reducing sugars and amino acids on proteins, lipids, and nucleic acids. Diet is a major external source of AGEs, with about 10% of AGEs from food remaining in the body after ingestion 20; bodily AGE leve ...
Appendix A: General Remarks on Handling RNA
... The ratio of the readings at 260 nm and 280 nm (A260/A280) provides an estimate of the purity of RNA with respect to contaminants that absorb in the UV, such as protein. However, the A260/A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/A280 rat ...
... The ratio of the readings at 260 nm and 280 nm (A260/A280) provides an estimate of the purity of RNA with respect to contaminants that absorb in the UV, such as protein. However, the A260/A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/A280 rat ...
Diapositiva 1
... Microsatellites are molecular marker loci consisting of tandem repeat units of very short (1-5 basepairs) nucleotide motif. In case the nucleotide sequences in the flanking regions of the microsatellite are known, specific primers (generally 20-25 bp) can be designed to amplify the microsatellite by ...
... Microsatellites are molecular marker loci consisting of tandem repeat units of very short (1-5 basepairs) nucleotide motif. In case the nucleotide sequences in the flanking regions of the microsatellite are known, specific primers (generally 20-25 bp) can be designed to amplify the microsatellite by ...
Amino Acids
... Most -Amino Acids are Chiral • The -carbon has always four substituents and is tetrahedral • All (except proline) have an acidic carboxyl group, a basic amino group, and an alpha hydrogen connected to the -carbon • Each amino acid has an unique fourth substituent R • In glycine, the fourth subst ...
... Most -Amino Acids are Chiral • The -carbon has always four substituents and is tetrahedral • All (except proline) have an acidic carboxyl group, a basic amino group, and an alpha hydrogen connected to the -carbon • Each amino acid has an unique fourth substituent R • In glycine, the fourth subst ...
CURRICULUM VITAE Personal Data Education and
... MSc’s thesis title: "Surface Enhanced Resonance Raman Spectroscopy as an analytical tool" ...
... MSc’s thesis title: "Surface Enhanced Resonance Raman Spectroscopy as an analytical tool" ...
Bio-Tech - AgriLife Extension County Offices
... Scientists have discovered that each gene carries the code for a specific, assigned function (such as whether a pea is wrinkled or smooth).Genetic engineering is the process in which a specific gene is taken from the DNA of one organism and inserted into the DNA of another, or a specific gene is rep ...
... Scientists have discovered that each gene carries the code for a specific, assigned function (such as whether a pea is wrinkled or smooth).Genetic engineering is the process in which a specific gene is taken from the DNA of one organism and inserted into the DNA of another, or a specific gene is rep ...
Cytochrome C Comparison Lab Purpose: To compare the
... Purpose: To compare the relatedness between organisms by examining the amino acid sequence in the protein Cytochrome C. Background: Cytochrome C is a protein involved in using energy in the cell. Cytochrome C is found in most, if not all, eukaryotes. Over time, random mutations in the DNA sequence o ...
... Purpose: To compare the relatedness between organisms by examining the amino acid sequence in the protein Cytochrome C. Background: Cytochrome C is a protein involved in using energy in the cell. Cytochrome C is found in most, if not all, eukaryotes. Over time, random mutations in the DNA sequence o ...
Upwelling, Downwelling, and El Nino
... 1st generation eliminated conservative, but not dispersive 2nd generation eliminated dispersive; only one band would have occurred if dispersive replication ...
... 1st generation eliminated conservative, but not dispersive 2nd generation eliminated dispersive; only one band would have occurred if dispersive replication ...
Automation of Reverse Transcription of Avian Influenza Viral RNA
... influenza virus- the virus found chiefly in birds. However, natural infections with influenza A viruses have been reported in a variety of animal species including humans, pigs and birds1. In the ongoing effort to prevent widespread disease, robotic workstations with higher throughput can be used to ...
... influenza virus- the virus found chiefly in birds. However, natural infections with influenza A viruses have been reported in a variety of animal species including humans, pigs and birds1. In the ongoing effort to prevent widespread disease, robotic workstations with higher throughput can be used to ...
biotechnology
... • Genome variations are differences in the sequence of DNA among individuals. They include both polymorphisms and mutations. • A polymorphism is a clinically harmless DNA variation. It often occurs in the intervening sequences that do not code for proteins • Mutation refers to an infrequent potentia ...
... • Genome variations are differences in the sequence of DNA among individuals. They include both polymorphisms and mutations. • A polymorphism is a clinically harmless DNA variation. It often occurs in the intervening sequences that do not code for proteins • Mutation refers to an infrequent potentia ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.