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Final
Final

... parenthesis that most accurately completes the statement. (1 point each). The study of variation in bacteria has several features that are distinct from the study of genetics in eukaryotic organisms. Bacteria typically have (a single, two, multiple) chromosome(s) that is(are) composed of (single str ...
LS1a Fall 2014 Practice Problem Set 6 1. Name three ways in which
LS1a Fall 2014 Practice Problem Set 6 1. Name three ways in which

... that enable certain bacteria to pump a broad spectrum of toxic compounds out of their cells. Berberine (drawn in bold) is a natural antimicrobial compound that binds to a bacterial protein called Qac R to cause enhanced transcription of a specific membrane transporter gene. The binding pocket for be ...
Chapter 6B
Chapter 6B

... An enormous amount of DNA sequence information is available from genome sequencing and sequencing of cloned genes. This data is stored in data banks such as GenBank at the NIH in Bethesda, MD and the EMBL Sequence Data Base at the European Molecular Biology Laboratory in Heidelberg, Germany. Scienti ...
DNA and the Genome
DNA and the Genome

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SBI 3U Genetics Review Questions LG #1: DNA
SBI 3U Genetics Review Questions LG #1: DNA

... 1. Identify the three components of a DNA molecule. Describe the types of bonds that hold these components together. 2. Identify the structures of adenine, guanine, thymine, and cytosine. Which nucleotides pair-up? 3. What is the shape of a DNA molecule like? Describe it. 4. How does DNA condense fr ...
Test 1
Test 1

... ii. Each reaction yields 38 ATP. iii. ADP + P  ATP (Fig. 4.7) b. Steps i. Glycolysis 1. Glucose breaks down to pyruvic acid (C6  2C3) 2. ATP is made and electrons are also released. 3. Takes place in cytoplasm ii. Acetyl-CoA formation 1. Pyruvic acid is broken down to acetic acid (C2). 2. CO2 is r ...
Title goes here
Title goes here

... 1. Problems of metagenomic data (metagenomic data is the problem) (see IMG/M -> Using IMG/M -> About IMG/M -> Background for definitions) ...
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8.1 Condensation Polymers

... LO: I know the structure of a polyester. ...
Control of Gene Expression
Control of Gene Expression

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Detection of Cow Milk in Water Buffalo Cheese by SYBR Green Real

... in governing liquid is due to the cheese matrix’s exfoliation that slowly occurs during the whole preservation period. DNA was found in all experimental samples. Real time amplification of DNA from governing liquid proved the method’s actual applicability for species detection purposes. Hot-start PC ...
Transformation of the bacterium E. coli using a gene for green
Transformation of the bacterium E. coli using a gene for green

... profound changes. Examples are Agrobacterium tumefaciens (for plants) and HIV (for Humans). The bacterium you will be transforming, E.coli, lives in the human gut and is a relatively simple and well understood organism. Its genetic material consists mostly of one large circle of DNA 3-5 million base ...
Protein Structure
Protein Structure

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Using Molecular Markers in Plant Genetics
Using Molecular Markers in Plant Genetics

The Sea Change That`s Challenging Biology`s Central Dogma
The Sea Change That`s Challenging Biology`s Central Dogma

... billion or so pairs of nucleotides, the “letters” of the genetic code that make up the rungs of the double helix. A fertilized human egg begins life with the DNA in its genome, half from the mother and half from the father. From that an entire human being of some 10 trillion cells is programmed. Acc ...
Experimentally testing the hypothesis of a limited amino acid
Experimentally testing the hypothesis of a limited amino acid

... the present time, any change would be lethal, or at least very strongly selected against; and ii) The shape of the genetic code table was entirely a matter of chance. Theories that rationalize the evolution of the genetic code have been also proposed: e.g. Stereochemical interaction [2], Co-evolutio ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 11. How is the structure of glucose elucidated? 12. Explain the factors affecting the enzymatic action. 13. Describe the importance of flavones and flavanoids. 14. How is the presence of ES complexes determined? Explain. 15. Explain the different types of soil. 16. How is N-terminal of an amino acid ...
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b. Ketogenic amino acids

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Practical Molecular Biology and Genetic Engineering
Practical Molecular Biology and Genetic Engineering

A kinetic proofreading mechanism for disentanglement of
A kinetic proofreading mechanism for disentanglement of

... compared with our model. The horizontal axis shows the thermal equilibrium entanglement probability; the vertical axis shows the steady-state entanglement probability results in the presence of type II topoisomerases and ATP. The kinetic proofreading model (see text) is able to reduce the knotting p ...
Radioactivity in Life Sciences www.AssignmentPoint.com
Radioactivity in Life Sciences www.AssignmentPoint.com

... higher cost compared to P-32, as most of the bombarded P-31 will have acquired only one neutron, while only some will have acquired two or more. Its maximum specific activity is 5,118 Ci/mmol (189.4 PBq/mol).  Phosphorus-32 is widely used for labeling nucleic acids and phosphoproteins. It has the h ...
Deception Through Terminology - Part 1 of 7
Deception Through Terminology - Part 1 of 7

... species and will have the same DNA structure as one of its parents. Thus, by definition it will be fertile itself under normal circumstances and it will be the same species (but not necessarily the same breed). I suspect that everyone has seen a "mutt," which is a dog which does not look very attrac ...
Slide 1 - MacWilliams Biology
Slide 1 - MacWilliams Biology

... 9. The ribosome helps form a peptide bond between the first and second amino acids— methionine and phenylalanine. 10. The bond holding the first tRNA molecule to its amino acid is broken. 11. tRNA then moves into a third binding site, from which it exits the ribosome. 12. The ribosome then moves to ...
Pyrosequencing Technology
Pyrosequencing Technology

... • Apyrase of a specific grade has been selected to ensure that all dNTPs are degraded, including the alfa-thio-dATP which is used instead of dATP. This enzyme also hydrolyzes ATP. • The rate of dNTP degradation by apyrase is slower than the rate of dNTP incorporation by the polymerase, favouring suf ...
Chapter 3
Chapter 3

... a spindle of fibers that extends from pole to pole. The asters, spindle, centrioles, and microtubules are collectively called the mitotic spindle (or mitotic apparatus). As prophase continues, a second group of microtubules grows out from the kinetochore to the poles of the cell. These kinetochore m ...
EcoCyc: Encyclopedia of Escherichia coli genes and metabolism
EcoCyc: Encyclopedia of Escherichia coli genes and metabolism

... EcoCyc object that represents the gene for the tRNA. 33 tRNA synthetases, and the associated charging reactions, are also encoded as EcoCyc objects, where the tRNA objects are substrates in these reactions. Additional substrates include the charged tRNAs, which are also represented as distinct objec ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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