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Table of Contents - NAU jan.ucc.nau.edu web server
Table of Contents - NAU jan.ucc.nau.edu web server

... T (transfer) site is where tRNA + amino acids first attaches to the ribosome. The A (amino acid) site is there the tRNA anticodon binds to mRNA codon The P (polypetide) site is where the amino acids are bonded together. The E (exit) site is where the tRNA will leave the ribosome to pick up additiona ...
Identifying Genes in E. coli
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... Identifying Genes in E. coli Required for Susceptibility to Antisense Antibiotics Susan Puckett Mentor: Dr. Bruce Geller AVI BioPharma Howard Hughes Medical Institute ...
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...  If antibiotic has been produced, one or more will fail to grow Method 2  determiens yield  Grown in culture  Discs of filter paper are dipped into liquid medium where microbes have grown  discs placed on agar plates inoculated with bacteriaIf there is antibiotic on the dicsc, bacteria will no ...
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34 Lambda Appendix - RIT

... When λ enters a susceptible cell, the single-stranded tails anneal to generate a circle. The site formed by the single stranded tails is called COS (cohesive site) and the left and right arms become contiguous and form a single operon, the right hand operon. The central region comprises a second ope ...
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Chapter 1 Lecture Notes - Tacoma Community College

... 7. Organ System:A group of body parts that carries out a particular function in an organism ...
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[II] Molecular Techniques for Studying Control of Gene Expression (II).

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... vector but doesn't always work completely. Ligation of DNA in a mixture routinely results in a variety of potential products. 1. Only circularized plasmids will survive in the bacteria. This eliminates all ligation products that are not recircularized. 2. In addition to our gene of interest, the vec ...
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A Review on Y-Chromosomal based DNA Profiling and Bayesian

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...  Genomic libraries can be constructed with different types of vectors – Plasmid library: genomic DNA is carried by plasmids – Phage library: genomic DNA is incorporated into bacteriophage DNA ...
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... strand of a -sheet in which all of the hydrophilic residues would be on one face of the sheet, with all of the hydrophobic residues on the other face. An amphipathic -sheet would most likely be found on the surface of a protein, with the hydrophilic residues facing outward into the aqueous environ ...
Control of Gene Expression
Control of Gene Expression

... either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons – but also in directing development as well as gene expression in general ...
2012 jf lecture 2.pptx
2012 jf lecture 2.pptx

... •  Specific polypeptides- unique sequence of aa’s (as determined by the genetic code) •  Sequence of the aa polymer determines the 3D shape of the polypeptide •  Proteins are not just chains of aa’s, they are defined by their shape – interactions between backbone residues and R-groups •  A protein’s ...
Nucleic Acid Synthesis in the Neoplastic Cell
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... nitrogen mustards esterify the phosphate groups of DNA and R N A (~07). Polyfunctional alkylating agents can react with two different nuclcophilic centers to form a bridge between them. In dilute nucleic acid solutions, intramolecular crosslinking can take place with the formation of a more compact ...
IBS 602 - Office of Biomedical Education
IBS 602 - Office of Biomedical Education

... foundation of knowledge in molecular biology (concepts and techniques) necessary for advanced graduate courses and research in any of the six basic science departments of the UK College of Medicine. The course will be focused primarily on the molecular mechanisms that underlie the regulated expressi ...
DNA extraction from frozen fieldcollected and dehydrated herbarium
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... Herbarium collections are now an immense source for obtaining molecular data, and molecular systematic studies are no longer strictly dependent on cultures or fresh/frozen basidiomata collections. DNA extracted from herbarium material is of low molecular weight, but is still capable of being PCR amp ...
Cell Bio!!!!
Cell Bio!!!!

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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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