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Paper 2
Paper 2

... Cell 1 undergoes division to give rise to cells 2 and 3. Some alleles are indicated by letters. ...
chapter eighteen
chapter eighteen

... Viruses and bacteria are the simplest biological systems—microbial models in which scientists find life’s fundamental molecular mechanisms in their most basic, accessible forms. ...
Lifespan of Prokaryote Model Organism Escherichia coli K-12
Lifespan of Prokaryote Model Organism Escherichia coli K-12

... environments. Bacteria would be useful to determine the effects of age on single cells, but because bacteria reproduce asexually by binary cell fission (clonal replication), calculating the lifespan has proven elusive. Without the determination of a lifespan, age studies using bacteria have limited ...
SECTION B
SECTION B

... Cell 1 undergoes division to give rise to cells 2 and 3. Some alleles are indicated by letters. ...
STRAND1 - Bulletin - Sigma
STRAND1 - Bulletin - Sigma

... • The observed fraction of dsDNA resistant to digestion presumably represents material derived from unphosphorylated primers. Increasing the digestion period or amount of enzyme has little effect on the remaining double-stranded DNA. However, as long as there is a sufficient amount of ssDNA produced ...
The Polymerase Chain Reaction
The Polymerase Chain Reaction

Tiger beetles - Discover the Microbes Within!
Tiger beetles - Discover the Microbes Within!

... the life cycle of the 2 beetles. One paper found that in one species of tiger beetles found in the flood plains of a part of the Peruvian Amazon the life cycles for males and females differed, and the males had a shorter lifespan than the females. (Amorim et al, 1997) Though I don’t know yet if the ...
amino acids properties
amino acids properties

... neurotransmitter, i.e., trasnmission of impulses in the nervous system, Tryptophan is the precursor of a vitamin named nicotinic acid (B3). 3-As Source of Sulphur. Derived from the sulfur containing amino acids. 4- Amino acids are involved in many metabolic pathways such as in Gluconeogenesis where ...
Amino Acids and Peptides-chap 3
Amino Acids and Peptides-chap 3

... Which groups on amino acids react to form a peptide bond?  Individual amino acids can be linked by forming covalent bonds  PEPTIDE (amide) bond is formed between α carboxyl group of one amino acid and α amino group of another amino acid  Dipeptide, tripeptide, polypeptide (> 100 amino acids) ...
Forever 100bp Ladder Personalizer
Forever 100bp Ladder Personalizer

Genetics and Epigenetics of Human Disease
Genetics and Epigenetics of Human Disease

... the DNA ‘ladder’ consists of a pair of two from four chemicals (called nucleotide bases). The chemical cytosine (C) always pairs with guanine (G), and thymine (T) always pairs with adenine (A) (see Figure 1). This pairing rule means that the sequence of letters on one strand of DNA can be used to pr ...
plasmid
plasmid

... Transformed of interest can be distinguished by looking at the colour of the colony they make on agar media. Recombinants will be white, whereas non-recombinants will bed blue in colour. This is the most notable feature of pUC19. ...
Amino Acids
Amino Acids

Monstrous Mutations
Monstrous Mutations

... 1. Change a codon to one that encodes a different amino acid and cause a small change in the protein produced. For example, sickle cell anemia is caused by a substitution in the beta-hemoglobin gene, which alters a single amino acid in the protein produced. 2. Change a codon to one that encodes the ...
FAN: fingerprint analysis of nucleotide sequences
FAN: fingerprint analysis of nucleotide sequences

... overall motif match score of zero, even when all the other residue match scores were good. There are two major reasons why this situation can arise: first, the motif may contain relatively few members of the family (most commonly because all the members have yet to be discovered); second, base subst ...
Introduction to Cellular and Molecular Biology (BIOL 190)
Introduction to Cellular and Molecular Biology (BIOL 190)

... 3. Recognize the different kinds of isomers (structural, cis-trans, and enantiomers) 4. Describe the structure and basic characteristics of the following functional groups: hydroxyl, carbonyl, carboxyl, amino, sulfhydryl, phosphate, methyl 5. List the four major kinds of biological macromolecules (c ...
Post-transcriptional modifications Cap a
Post-transcriptional modifications Cap a

... Gene silencing is probably often the result of more than one mechanism. Transcriptional gene silencing (TGS) is often associated with methylation of the gene, which may inhibit transcription. In posttranscriptional gene silencing (PTGS), high levels of normal mRNA can cause activation of RNA-depende ...
Amino Acids and Their Properties
Amino Acids and Their Properties

Lecture 4
Lecture 4

... Table II.1.1. It is also possible to have other amino acids – compounds with the same backbone, but with different sidechains. Such non-natural amino acids are occasionally found in biological proteins. Since they are made of more than one monomer type, proteins are heteropolymers. Typically, a prot ...
Lecture 2: Mutation and its effect
Lecture 2: Mutation and its effect

... Mutation rates vary widely from one gene to another; mutational hot spots are more likely to be mutated than others ...
Transcription
Transcription

... regulatory DNA sequences (enhancers, silencers, HRE) lying on the same chromosome, distant from promoters (very often in large distance). They act as activators or repressors of the given gene transcription. Specific transcription factors interact with mediator proteins (coactivators, corepressors) ...
Cytogenetic and molecular characterization of the
Cytogenetic and molecular characterization of the

... 200 bp and a ladder of electrophoretic bands with molecular weights which are a multiple of 200 bp. Southern blotting revealed that this ladder is composed of DNA fragments that are multimers of the 200-bp DraI band suggesting that DraI isolated a satellite that has been called Mamestra brassicae sa ...
INTRODUCING AMINO ACIDS
INTRODUCING AMINO ACIDS

... diagram. The solid structure of the matrix retards the diffusion of the solute molecules, which will remain where they are inserted, unless acted upon by the electrostatic potential. In the example shown here, four different amino acids are examined simultaneously in a pH 6.00 buffered medium. To se ...
Simulating cellular dynamics through a coupled transcription
Simulating cellular dynamics through a coupled transcription

... that the faster these intermediate processes are, the smaller the time step required and therefore the increase in cpu time. Through the use of our slow manifold projection methodology, the algebra is automated for equilibrated and steady state fast processes with a wide range of complexities. In th ...
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements

... 1. Chemical mutagens may be naturally occurring, or synthetic. They form different groups based on their mechanism of action: a. Base analogs depend upon replication, which incorpocates a base with alternate states (tautomers) that allow it to base pair in alternate ways, depending on its state. i. ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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