View as PDF
... Modifications of Cas9-target sites can be achieved by supplying a homologous repair template in addition to sgRNA and Cas9. Homologous repair templates can be plasmids or oligonucleotides containing regions of homology surrounding the target sequence, that are altered to have the desired mutations. ...
... Modifications of Cas9-target sites can be achieved by supplying a homologous repair template in addition to sgRNA and Cas9. Homologous repair templates can be plasmids or oligonucleotides containing regions of homology surrounding the target sequence, that are altered to have the desired mutations. ...
Fermentation
... • Defined as an energy yielding process • Organic molecules serve as both electron donors and electron accepters. • The molecule being metabolized does not have all its potential energy extracted from it. (not completely oxidized.) ...
... • Defined as an energy yielding process • Organic molecules serve as both electron donors and electron accepters. • The molecule being metabolized does not have all its potential energy extracted from it. (not completely oxidized.) ...
EML4-ALK pre-mRNA and mature mRNA fusion detection using
... Gene fusions that activate otherwise silent signaling enzymes, such as the anaplastic lymphoma receptor tyrosine kinase (ALK), are responsible for a significant number of lung and other cancers. Several drugs that target ALK have found success in the treatment of patients with ALK-gene fusions. DN ...
... Gene fusions that activate otherwise silent signaling enzymes, such as the anaplastic lymphoma receptor tyrosine kinase (ALK), are responsible for a significant number of lung and other cancers. Several drugs that target ALK have found success in the treatment of patients with ALK-gene fusions. DN ...
Slides - Websupport1
... • Two pyruvates = 34 ATP • The chemical formula for this process is C6H12O6 + 6 O2 6 CO2 + 6 H2O ...
... • Two pyruvates = 34 ATP • The chemical formula for this process is C6H12O6 + 6 O2 6 CO2 + 6 H2O ...
Understanding Genetics: Punnett Squares
... document. Modifications can be made based on the needs of the students. Some classes may decide for each student to add individual components of the extraction buffer to the Ziploc bag (roughly 2 tsp water, 1 tsp soap, 1 pinch salt), while other classes may choose to use the teacher prepared extract ...
... document. Modifications can be made based on the needs of the students. Some classes may decide for each student to add individual components of the extraction buffer to the Ziploc bag (roughly 2 tsp water, 1 tsp soap, 1 pinch salt), while other classes may choose to use the teacher prepared extract ...
Peptide bonds, polypeptides and proteins printable pdf
... Water, polypeptide synthesis and folding: Were it not for the presence of hydrophobic R-groups, all polypeptides would assume an extended configuration in water. H-bond donors and acceptor groups in polypeptide backbone would form Hbonds with each other and with water molecules. More typical polypep ...
... Water, polypeptide synthesis and folding: Were it not for the presence of hydrophobic R-groups, all polypeptides would assume an extended configuration in water. H-bond donors and acceptor groups in polypeptide backbone would form Hbonds with each other and with water molecules. More typical polypep ...
Chapter Assessment
... Writing chemical equations • A molecule of table sugar can be represented by the formula: C12H22O11. • The easiest way to understand chemical equations is to know that atoms are neither created nor destroyed in chemical reactions. ...
... Writing chemical equations • A molecule of table sugar can be represented by the formula: C12H22O11. • The easiest way to understand chemical equations is to know that atoms are neither created nor destroyed in chemical reactions. ...
Amino-Form
... When ammonium and nitrates are absorbed by roots or leaves, they are first converted into Glutamic acid, one of the most important Lform amino acids. All the other amino acids are then formed by the reaction of organic molecules with Glutamic acid. A percentage of these amino acids will combine to f ...
... When ammonium and nitrates are absorbed by roots or leaves, they are first converted into Glutamic acid, one of the most important Lform amino acids. All the other amino acids are then formed by the reaction of organic molecules with Glutamic acid. A percentage of these amino acids will combine to f ...
Student
... weather like hurricanes or floods. Your list may also include threats due to microbes such as bacteria or fungi that may break down some of the structure of the boat. Regardless of the threats you will face, it is clear that there will be some maintenance required. Look at your answer to the second ...
... weather like hurricanes or floods. Your list may also include threats due to microbes such as bacteria or fungi that may break down some of the structure of the boat. Regardless of the threats you will face, it is clear that there will be some maintenance required. Look at your answer to the second ...
The Master Molecule of Life
... material could be transferred from one bacterial cell to another. Griffith also discovered that the transferred DNA could alter the traits of the recipient cells. Griffith was studying two strains of bacteria: one that caused pneumonia in mice and another that did not. When grown in a culture dish, ...
... material could be transferred from one bacterial cell to another. Griffith also discovered that the transferred DNA could alter the traits of the recipient cells. Griffith was studying two strains of bacteria: one that caused pneumonia in mice and another that did not. When grown in a culture dish, ...
IN VITRO TRANSCRIPTION . TRANSLATION - UTH e
... The bacteriophage promoters, T7, T3, and SP6, consist of 23 basepairs numbered -17 to +6, where +1 indicates the first base of the coded transcript. An important observation is that, of the +1 through +6 bases, only the base composition of +1 and +2 are critical and must be a G and purine, respectiv ...
... The bacteriophage promoters, T7, T3, and SP6, consist of 23 basepairs numbered -17 to +6, where +1 indicates the first base of the coded transcript. An important observation is that, of the +1 through +6 bases, only the base composition of +1 and +2 are critical and must be a G and purine, respectiv ...
Uric acid estimation in plasma
... Absence of HGPRT is cause of Lesch-Nyhan syndrome This increase may be due to PRPP feed-forward activation of de novo pathways the rate of purine synthesis is increased about 200X Symptoms are gouty arthritis due to uric acid accumulation and severe neurological malfunctions including mental retarda ...
... Absence of HGPRT is cause of Lesch-Nyhan syndrome This increase may be due to PRPP feed-forward activation of de novo pathways the rate of purine synthesis is increased about 200X Symptoms are gouty arthritis due to uric acid accumulation and severe neurological malfunctions including mental retarda ...
Degenerate PCR - Yale School of Medicine
... Inosine is a purine (which occurs naturally in tRNAs) that can form base pairs with cytidine, thymidine, and adenosine (although the inosine:adenosine pairing presumably doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalty to pay when the helix bulges out at this ...
... Inosine is a purine (which occurs naturally in tRNAs) that can form base pairs with cytidine, thymidine, and adenosine (although the inosine:adenosine pairing presumably doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalty to pay when the helix bulges out at this ...
Gwy-Am Shin
... • Negative results don’t necessarily mean target not there • There is a need to focus on the reliability and sensitivity of concentration methods • Difficulties with a single platform for any one media because of wide range of organisms and environmental conditions ...
... • Negative results don’t necessarily mean target not there • There is a need to focus on the reliability and sensitivity of concentration methods • Difficulties with a single platform for any one media because of wide range of organisms and environmental conditions ...
STEM-ED Genetics pathway
... In sexual reproduction, a sperm cell from a male unites with an egg cell from a female. Sperm and egg cells are specialised cells each of which has one of the two versions of each gene carried by the parent, selected at random. When a sperm and egg cell combine one full set of genes in the fertilise ...
... In sexual reproduction, a sperm cell from a male unites with an egg cell from a female. Sperm and egg cells are specialised cells each of which has one of the two versions of each gene carried by the parent, selected at random. When a sperm and egg cell combine one full set of genes in the fertilise ...
A novel procedure for genotyping of single nucleotide polymorphisms in trisomy with genomic DNA and the invader assay.
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
... can accurately determine genotypes in trisomic DNA samples are expensive, require specialized equipment and complicated analyses. Here we report proof-of-concept results for an InvaderÕ assay-based genotyping procedure that can determine SNP genotypes in trisomic genomic DNA samples in a simple and ...
RECOMBINANT-DNA METHODOLOGY
... there can be only one (a unique restriction site), or there can be none. It all depends on the sequence of the specific piece of DNA in question. Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces ...
... there can be only one (a unique restriction site), or there can be none. It all depends on the sequence of the specific piece of DNA in question. Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces ...
4 Titration Curve of an Amino Acid
... A few amino acids are classified as triprotic. This is because, in addition to the ionizable protons of the α-COOH and α-NH3 groups, they also have a dissociable proton in their R group. Although triprotic amino acids can exist as zwitterions, under physiological conditions these amino acids will be ...
... A few amino acids are classified as triprotic. This is because, in addition to the ionizable protons of the α-COOH and α-NH3 groups, they also have a dissociable proton in their R group. Although triprotic amino acids can exist as zwitterions, under physiological conditions these amino acids will be ...
WYSE – “Academic Challenge” - Worldwide Youth in Science and
... © 2006 Worldwide Youth in Science and Engineering “WYSE”, “Worldwide Youth in Science and Engineering” and the “WYSE Design” are service marks of and this work is Copyright © 2006 Board of Trustees of the University of Illinois at Urbana - Champaign. ...
... © 2006 Worldwide Youth in Science and Engineering “WYSE”, “Worldwide Youth in Science and Engineering” and the “WYSE Design” are service marks of and this work is Copyright © 2006 Board of Trustees of the University of Illinois at Urbana - Champaign. ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.