Laboratory of Dr. Wayne L. Hubbell Protocol designed by Carlos J
... overnight incubation at 37°C, select single colonies and inoculate into 20 mL of starter LB medium containing the aforementioned antibiotics and then grow overnight at 37 °C in a shaking incubator. The following day, inoculate the starter culture into 1-L of LB medium containing the aforementioned a ...
... overnight incubation at 37°C, select single colonies and inoculate into 20 mL of starter LB medium containing the aforementioned antibiotics and then grow overnight at 37 °C in a shaking incubator. The following day, inoculate the starter culture into 1-L of LB medium containing the aforementioned a ...
Work Day 1
... 2. Antibiotics are changing and subsequently becoming weaker – Antibiotics are inert chemical substances. Bacteria, however, are living organisms that can change through evolution. 3. Bacteria have mutated in order to become resistant – Bacteria do not “choose” to mutate, adapt, or develop resistanc ...
... 2. Antibiotics are changing and subsequently becoming weaker – Antibiotics are inert chemical substances. Bacteria, however, are living organisms that can change through evolution. 3. Bacteria have mutated in order to become resistant – Bacteria do not “choose” to mutate, adapt, or develop resistanc ...
General Biochemistry Chemistry of Polysaccharides
... Cellulose, a fibrous, tough, water-insoluble substance, is found in the cell walls of plants. Cellulose constitutes much of the mass of wood, and cotton is almost pure ...
... Cellulose, a fibrous, tough, water-insoluble substance, is found in the cell walls of plants. Cellulose constitutes much of the mass of wood, and cotton is almost pure ...
Slide 1
... Fat catabolism: generation of energy by fatty acid oxidation Fat (triacylglycerol) and Fatty Acids: 90% of dietary lipids are tryacylglycerol, a hydrophobic, neutral molecule made from reaction of OH group of glycerol and COO- group of fatty acids. Fatty acids are made up of a long hydrophobic hydro ...
... Fat catabolism: generation of energy by fatty acid oxidation Fat (triacylglycerol) and Fatty Acids: 90% of dietary lipids are tryacylglycerol, a hydrophobic, neutral molecule made from reaction of OH group of glycerol and COO- group of fatty acids. Fatty acids are made up of a long hydrophobic hydro ...
Organic Chemistry
... • Two conformations are possible for a planar peptide bond. • Virtually all peptide bonds in naturally occurring proteins studied to date have the s-trans conformation. ...
... • Two conformations are possible for a planar peptide bond. • Virtually all peptide bonds in naturally occurring proteins studied to date have the s-trans conformation. ...
Full text - Caister Academic Press
... DNAs with a GC content higher than the GC content of their own genome. Bacterial and archaeal genomes with high AT content are protected from attacks by most viruses. On the other hand, it is difficult for those organisms to use any plasmids. The genome sizes of obligate host-associated bacteria are ...
... DNAs with a GC content higher than the GC content of their own genome. Bacterial and archaeal genomes with high AT content are protected from attacks by most viruses. On the other hand, it is difficult for those organisms to use any plasmids. The genome sizes of obligate host-associated bacteria are ...
DNA Barcoding of Algae and Bacteria from
... the NCBI website was used to analyze the DNA sequences resulting from sequencing. Bioinformatics programs were then used to identify the different species of Algae in different environments based on the BLAST results analysis. DAY 2 ...
... the NCBI website was used to analyze the DNA sequences resulting from sequencing. Bioinformatics programs were then used to identify the different species of Algae in different environments based on the BLAST results analysis. DAY 2 ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
... Therefore, DNA molecule I is the least sensitive, while molecule III is the most sensitive. 24. Frameshift mutations are caused by insertions or deletions of bases (that are not multiples of 3). These will shift the reading frame for all codons downstream from the mutation. Single base-substitutions ...
Amino acid a
... • Chiral (from Greek cheir, meaning “hand”): – An object or a system cannot be superimposed on its mirror image. – One hand does not match the other when superimposed. ...
... • Chiral (from Greek cheir, meaning “hand”): – An object or a system cannot be superimposed on its mirror image. – One hand does not match the other when superimposed. ...
Full Text
... emphasizes sequence variation within proteins of common structure or function. Along with the amino acids conserved at any one site, we have looked specifically for conserved correlations between amino acids at two distinct sites in a motif. We felt that if the structure of a motif was highly conser ...
... emphasizes sequence variation within proteins of common structure or function. Along with the amino acids conserved at any one site, we have looked specifically for conserved correlations between amino acids at two distinct sites in a motif. We felt that if the structure of a motif was highly conser ...
Structural analysis of both products of a reciprocal translocation
... F1g. 3. Genomic representation of the reciprocally translocated junction fragment and the aberrantly rearranged J5 segment. A) Ten pg of genomic DKA from normal human polymorphonuclear cells (control) and the Burk i t t lynphoma cell Une BL22 are digested with HindiII and compared to 20 picograras D ...
... F1g. 3. Genomic representation of the reciprocally translocated junction fragment and the aberrantly rearranged J5 segment. A) Ten pg of genomic DKA from normal human polymorphonuclear cells (control) and the Burk i t t lynphoma cell Une BL22 are digested with HindiII and compared to 20 picograras D ...
Interaction
... Oct-1 activates transcription of genes that are involved in basic cellular processes Oct-1 activates small nuclear RNA (snRNA) and ...
... Oct-1 activates transcription of genes that are involved in basic cellular processes Oct-1 activates small nuclear RNA (snRNA) and ...
Amino Acids Proteins, and Enzymes
... Add protecting group to prevent formation of new disulfide bonds ...
... Add protecting group to prevent formation of new disulfide bonds ...
PTC_241Lecture005Cells
... on DNA you put a guanine in the RNA but now for the adenine in DNA it pairs with uricile in RNA. So now this becomes the messenger RNA. And then it has to leave the nucleus in order to help make a protein through that last process which is what we call translation. So what would you cal this proces ...
... on DNA you put a guanine in the RNA but now for the adenine in DNA it pairs with uricile in RNA. So now this becomes the messenger RNA. And then it has to leave the nucleus in order to help make a protein through that last process which is what we call translation. So what would you cal this proces ...
Regulation of DNA Polymerase Exonucleolytic Proofreading Activity
... DNA polymerases (Gauss et al. 1983; Beauchamp and Richardson 1988) due to increased exonucleolytic proofreading, which removes correct nucleotides in addition to incorrect nucleotides (Muzyczka et al. 1972; Gillin and Nossal, 1976a; reviewed in Goodman et al. 1993). Another potential disadvantage of ...
... DNA polymerases (Gauss et al. 1983; Beauchamp and Richardson 1988) due to increased exonucleolytic proofreading, which removes correct nucleotides in addition to incorrect nucleotides (Muzyczka et al. 1972; Gillin and Nossal, 1976a; reviewed in Goodman et al. 1993). Another potential disadvantage of ...
Similarity
... propensity in a 4 residue window falls below a value. Output-helix, strand or turn. ...
... propensity in a 4 residue window falls below a value. Output-helix, strand or turn. ...
Isolating, Cloning, and Sequencing DNA
... (discussed below). The second procedure uses the bacteriophage enzyme polynucleotide kinase to transfer a single 32Plabeled phosphate from ATP to the 5 end of each DNA chain (Figure 8-24B). Because only one 32P atom is incorporated by the kinase into each DNA strand, the DNA molecules labeled in thi ...
... (discussed below). The second procedure uses the bacteriophage enzyme polynucleotide kinase to transfer a single 32Plabeled phosphate from ATP to the 5 end of each DNA chain (Figure 8-24B). Because only one 32P atom is incorporated by the kinase into each DNA strand, the DNA molecules labeled in thi ...
microbial genetics
... (iv) Many types of mutants that fit the above criteria will show segregation during mitotic division. This is very common in variegated plants that carry more than one type of plastid (chloroplast) per cell. This leads to variegation, suggesting somatic or vegetative segregation of the plastid types ...
... (iv) Many types of mutants that fit the above criteria will show segregation during mitotic division. This is very common in variegated plants that carry more than one type of plastid (chloroplast) per cell. This leads to variegation, suggesting somatic or vegetative segregation of the plastid types ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.