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video slide - Greenwood School District 50 / Overview
video slide - Greenwood School District 50 / Overview

... TECHNIQUE The starting materials for PCR are doublestranded DNA containing the target nucleotide sequence to be copied, a heat-resistant DNA polymerase, all four nucleotides, and two short, single-stranded DNA molecules that serve as primers. One primer is complementary to one strand at one end of t ...
1. The figure below represents a water molecule. H O H Water
1. The figure below represents a water molecule. H O H Water

... State the letter, X, Y or Z, that most accurately represents the enzyme DHPS. ...
Common Pattern of Coarse-Grained Charge Distribution of
Common Pattern of Coarse-Grained Charge Distribution of

Protein design as an inverse problem
Protein design as an inverse problem

... – Work in the Green lab is on-going to design proteins for use as anti-HIV prophylatics. ...
Map Quest: New Techniques Reveal How the
Map Quest: New Techniques Reveal How the

... A few years later, Dr. Burkitt sent tumor samples to Michael Anthony Epstein, who thought a virus might be causing the children’s jaw cancers. Dr. Epstein found virus particles for what is now known as Epstein-Barr virus (EBV) in some of the tumor cells, providing the first evidence that endemic Bur ...
The trimethoprim-resistant dihydrofolate reductase associated with
The trimethoprim-resistant dihydrofolate reductase associated with

... The gene is 234 bp long and codes for 78 amino acids. No homology can be found between the deduced amino acid sequence of the R388 dihydrofolate reductase and those of other prokaryotic or eukaryotic dihydrofolate reductases. However, it differs in only 17 positions from the enzyme associated with t ...
PDF
PDF

... (2000) named this polymorphic site as S 2I 2 variant due to the presence of the I 2 allele. However, an additional duplication of the inserted 10 bp sequence (the S 2 I 3 variant) has not been recognized in any of the studied animals. Kumar et al. (2006) examined these alterations at both DNA and pr ...
amino acid
amino acid

... shape. Such proteins are made up of long rod-shaped or stringlike molecules that can intertwine with one another and form strong fibers. They are water-insoluble and generally have structural functions within the human body. • А globular protein is а protein whose overall shape is roughly spherical ...
5   end
5 end

... • Three properties of RNA enable it to function as an enzyme – It can form a three-dimensional structure because of its ability to base pair with itself – Some bases in RNA contain functional groups – RNA may hydrogen-bond with other nucleic acid molecules ...
Sidney Markowitz PhD Research Proposal
Sidney Markowitz PhD Research Proposal

... proteins, organize itself into a stable selfreproducing, further evolving unit?” One of his results was to elucidate the notion of an error threshold in replication as a solution to the error catastrophe problem raised by Orgel (1963; 1970). (Hoffmann, 1974) applied a similar threshold to the proces ...
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Slide

... Which representative of the species? Which human? ...
CHAPTER 15 ACIDS AND BASES
CHAPTER 15 ACIDS AND BASES

... Table 15.2 of the text contains a list of important Brønsted acids and bases. (a) both (why?), (b) base, (c) acid, (d) base, (e) acid, (f) base, (g) base, (h) base, (i) acid, (j) acid. ...
review_for_midterm_2016
review_for_midterm_2016

...  What is the endocrine system and how does it work?  Be able to discuss specific glands, their location (be able to label them on a diagram-see page 998 in your text) and their functions (what hormones do they release and what do these hormones do).  Be able to explain the difference between ster ...
notes - local.brookings.k12.sd.us
notes - local.brookings.k12.sd.us

... describe and give examples of chemical reactions required to sustain life (hydrolysis, dehydration synthesis, photosynthesis, cellular respiration, ADP/ATP, role of ...
Large scale features in DNA genomic signals
Large scale features in DNA genomic signals

... one-directional sequence of nucleotides. The repetitive unit, the nucleotide, is made up of three parts: a phosphate group, the deoxyribose—a sugar stripped of a certain oxygen atom, and a nitrogenous base linked by strong covalent bounds. There are four kinds of nucleotides that di2er by their nitr ...
Organic Chemistry: Polymerization Reactions
Organic Chemistry: Polymerization Reactions

... What properties make Teflon a good product for non-stick materials?  Teflon is made up of C-F bonds which are very strong (not C-H bonds). These very strong bonds make the Teflon highly unreactive (non-sticking), it has a high melting point and it has a slippery surface ...
Solutions to Genetics Day 6 Interpretation Questions
Solutions to Genetics Day 6 Interpretation Questions

... the P1 phage found in the transducing lysates will have packaged bacterial DNA. Also: ( *Not required) a tiny % the P1 phage used to infect the mutants will have host DNA) Of the phage stocks used in the lab, which did you use at high MOI? Which did you use at low MOI? Explain why. a) The modified λ ...
Global MAPS Metabolomic Assisted Pathway Screen
Global MAPS Metabolomic Assisted Pathway Screen

... Mitochondria are structures within cells that convert the energy from food into a form that cells can use. Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. This genetic material is known as mitochondrial DNA or mtDNA. In humans, ...
Cloning genes into the AdZ vectors and making
Cloning genes into the AdZ vectors and making

... conditions than the real positives. Once you’ve seen the difference it’s quite obvious & you should have no problems picking the real white ones. If you do an uninduced sample of bacteria you can compare the uninduced plate to the induced using this method & you should easily be able to see that the ...
Brooker Chapter 10 - Volunteer State Community College
Brooker Chapter 10 - Volunteer State Community College

... information required to produce an organism ...
Tech Notes Mutagenesis of Amplified DNA Sequences Using Ampligase
Tech Notes Mutagenesis of Amplified DNA Sequences Using Ampligase

Detecting multiple DNA human profile from a mosquito blood meal
Detecting multiple DNA human profile from a mosquito blood meal

... removed. To simulate a hypothetical case: volunteer A was defined as the victim, and the other volunteers (B, C and D) were designated as would be possible suspects. After completion of the blood feeding, the mosquitoes were sacrificed 24, 48 and 72 h following the blood meal. The mosquitoes were cr ...
Proteins in the Diet - Nutrition and Food Technology-just
Proteins in the Diet - Nutrition and Food Technology-just

Introducing: TGGE
Introducing: TGGE

... The DNA fragments are separated by their melting behavior. They can be distinguished as soon as the fragments begin to melt, i.e. they form a fork like structure. During electrophoresis the fragments should not separate into single strands. This is an irreversible transition resulting in diffuse ban ...
Ironing Out Jesse`s Problem
Ironing Out Jesse`s Problem

... for both segments allowing for 1-2 mistakes. AND Student accurately describes transcription and translation in the cell in writing and/or labeled diagrams. Minor errors do not detract from meaning. LS1 (9-11)-2a and c ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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