• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
TOPIC 6.3
TOPIC 6.3

... • Enzyme-linked immunosorbent assays (ELISA) are used to detect the presence or absence of a particular protein • For example, people recently infected with HIV will initially produce antibodies against HIV. These antibodies can be detected by ELISA to determine if a patient is HIV positive or negat ...
carson and gavy doc
carson and gavy doc

... interactions between the hormones and nerves create a system of coordinated cycles for almost all of the functions in the body. Such as when the nerves excite certain glands and excrete hormones. Hormones are involved in transmitting signals back to nerve cells as well. Some of these hormones can af ...
The Unforgetables of Biology
The Unforgetables of Biology

...  The Cell Theory states that cells: all living things are made of cells, cells are the basic units of structure and function, and all cells come from other cells. The main tool that allowed for the discovery of cells and cell theory was the compound light microscope.  There are two main types of c ...
Objective 2 - Organization of Living Systems
Objective 2 - Organization of Living Systems

... To be closely related means the amino acid composition should be almost the same, since that is what the DNA is coding.  Between Q and T, only 4 levels are the same –  Between R and S only 4 levels are the same –  Between Q and S 5 of the levels are the same, but –  Between Q and R 5 of the lev ...
Cells: Beyond the Membrane
Cells: Beyond the Membrane

... b. Lysosomes Contain acidic digestive enzymes = can digest almost all biological materials Abundant in phagocytes Functions include: – Degrading old, dying, & non-functional organelles & tissues – Digesting foreign materials – Breaking down bone to release Ca2+ into blood ...
UNIT ONE - Cells and Heredity
UNIT ONE - Cells and Heredity

... WHITE – part of immune system that keeps us healthy in killing destructive agents to the body PLATLETS – The clotting parts that seal wounds ...
Organ Systems and Life
Organ Systems and Life

... survive. The organs can be divided into reproductive and vegetative. Flower: The flower is the reproductive organ of plants. It may contain the stamen and or the carpel. The stamen contains the pollen, which is brought to the carpel by insects. The carpel contains the ovaries. The receptor of pollen ...
Cells and tissues
Cells and tissues

... • secretes straight into blood stream ...
Definitions handout
Definitions handout

... A toxic chemical produced in anaerobic respiration, it produces cramp. Released in respiration & required for other activities. A high energy chemical used in cells as an energy store. The central nervous system consists of the brain and spinal cord. This is a nerve cell. It carries an electrical me ...
Physiology 2008
Physiology 2008

... When a group of cells with similar structure are found together performing a common function and possess similar extra-cellular substances located between the cells the result is a tissue. The microscopic study of tissue structure is called histology, which you have been and will continue to cover i ...
Life Science: Cells
Life Science: Cells

... Computer with Internet access ...
Cells are the
Cells are the

... Greater the SURFACE, more stuff gets ___________ of cell Greater the VOLUME, less stuff gets in or out; cell _________or is _______________ ...
Spring Final Exam Review Questions
Spring Final Exam Review Questions

... c. to analyze information b. to process information d. all of the above ____ 30. Which division(s) of the peripheral nervous system transmit(s) impulses from sense organs to the central nervous system? a. sensory division c. sensory and motor divisions b. motor division d. spinal cord division ...
Chap 20 – Organization of Multicellular Organisms
Chap 20 – Organization of Multicellular Organisms

... exchange materials across the plasma membrane  Cell size must – be large enough to house DNA, proteins, and structures needed to survive and reproduce, but – remain small enough for a SA:volume ratio that will allow for adequate exchange with the environment. ...
Biology Quiz Review – Science 8 Introduction to Cells, Tissues
Biology Quiz Review – Science 8 Introduction to Cells, Tissues

... 7. What is a Vacuole? Membrane-bound organelle found mainly in plant cells, but also in some animal cells. They are like storage bubbles found in cells. They store nutrients and waste products and help keep the shape of the cell. 8. What are Ribosomes? The part of the cell that helps to make protein ...
File - Mr. Downing Science 10
File - Mr. Downing Science 10

... gives the cell structure – the organelles are suspended in the cytoplasm and can move around through the process of cytoplasmic streaming – stores nutrients until organelles are ready to use them ...
Cell - centralmountainbiology
Cell - centralmountainbiology

... microtubules (hollow tubes) and microfilaments (threads made out of actin) • Function: -acts as a support system for organelles -maintains cell shape ...
Specialized Cells - Savita Pall and Chemistry
Specialized Cells - Savita Pall and Chemistry

... This is because the original cell became specialized as it divided and multiplied through mitosis, which results in daughter cells with identical genetic information. As the human body grows, cells that start out being the same undergo cell differentiation, which results in cells becoming specialize ...
printer-friendly sample test questions
printer-friendly sample test questions

... A. Students must define a unicellular organism as an organism which carries on all the functions of life, but is composed of only one cell. B. Students should include the fact that unicellular organisms consist of one cell and multicellular organisms are composed of more than one cell. Both types of ...
Biology Midterm Review Sheet- 2016
Biology Midterm Review Sheet- 2016

... what each discovered / contributed. 4. What are the three principles of the cell theory? 5. Why are microscopes a crucial piece of technology when studying cells? 6. Go back to unit one. What the three main types of microscopes? Describe the characteristics of each one. 7. List the three domains. 8. ...
Cell - Tri-City
Cell - Tri-City

... Cell Hall of Fame What is the cell theory? • All organisms are made up of one or more cells. • The cell is the basic unit of all organisms. • All cells come from existing cells. ...
The Cell
The Cell

... To be closely related means the amino acid composition should be almost the same, since that is what the DNA is coding.  Between Q and T, only 4 levels are the same –  Between R and S only 4 levels are the same –  Between Q and S 5 of the levels are the same, but –  Between Q and R 5 of the lev ...
Levels of Cellular Organization
Levels of Cellular Organization

...  Transmits communication throughout the body.  Two Types: Central Nervous System: Forms the brain and spinal cord  Peripheral Nervous System: Cranial Nerves and Spinal Nerves as well as the motor neurons ...
Cells of the Body
Cells of the Body

... Cells come in a variety of shapes and sizes. Typical cells range from 5 to 50 micrometers. Despite the difference in sizes, all cells have two characteristics in common. They are all surrounded by a cell membrane and all cells contain genetic material. Cells in multicellular organisms are specialize ...
Lymphatic System Test
Lymphatic System Test

... ______14. Specialized lymph nodes located in the intestines that help protect against invading organisms in the digestive tract are known as: a. T cells b. lymphocytes c. Peyer’s patches ______ 15. The __________ T cell remembers an invading antigen and is ready to fight that same antigen if it is e ...
< 1 ... 58 59 60 61 62 63 64 65 66 ... 80 >

Neuronal lineage marker



A Neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells as neurons. It allows detection and identification of cells by using different techniques. A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest. It can also be a protein tag, as a partial protein, a protein or a epitope that discriminates between different cell types or different states of a common cell. An ideal marker is specific to a given cell type in normal conditions and/or during injury. Cell markers are very valuable tools for examining the function of cells in normal conditions as well as during disease. The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells.The techniques used for its detection can be immunohistochemistry, immunocytochemistry, methods that utilize transcriptional modulators and site-specific recombinases to label specific neuronal population, in situ hybridization or fluorescence in situ hybridization (FISH). A neuronal lineage marker can be a neuronal antigen that is recognized by an autoantibody for example Hu, which is highly restricted to neuronal nuclei. By immunohistochemistry, anti-Hu stains the nuclei of neurons. To localize mRNA in brain tissue, one can use a fragment of DNA or RNA as a neuronal lineage marker, a hybridization probe that detects the presence of nucleotide sequences that are complementary to the sequence in the probe. This technique is known as in situ hybridization. Its application have been carried out in all different tissues, but particularly useful in neuroscience. Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations.Although immunohistochemistry is the staple methodology for identifying neuronal cell types, since it is relatively low in cost and a wide range of immunohistochemical markers are available to help distinguish the phenotype of cells in the brain, sometimes it is time-consuming to produce a good antibody. Therefore, one of the most convenient methods for the rapid assessment of the expression of a cloned ion channel could be in situ hybridization histochemistry.After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report