Why Sequence Alignment?
... 2. Learn about evolutionary relationships – • Two sequences from different organisms are similar they may have a common ancestor. • Needed for construction of phylogenetic trees ...
... 2. Learn about evolutionary relationships – • Two sequences from different organisms are similar they may have a common ancestor. • Needed for construction of phylogenetic trees ...
Specific and ubiquitous expression of different Zn finger protein
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
... Many eucaryotic genes involved in the process of differentiation and development are expressed in a temporally and spatially restricted fashion(15). Considering that • regulation of such genes also occur at the transcriptional level,the identification of different transcriptional regulatory factors ...
Fungal denitrification and nitric oxide reductase cytochrome P450nor
... occurrence of denitrification was previously thought to be restricted to bacteria (prokaryotes), and have characterized the fungal denitrification system. It comprises NirK (copper-containing nitrite reductase) and P450nor (a cytochrome P450 nitric oxide (NO) reductase (Nor)) to reduce nitrite to ni ...
... occurrence of denitrification was previously thought to be restricted to bacteria (prokaryotes), and have characterized the fungal denitrification system. It comprises NirK (copper-containing nitrite reductase) and P450nor (a cytochrome P450 nitric oxide (NO) reductase (Nor)) to reduce nitrite to ni ...
Slide 1
... •Prohormone convertases (PCs) are enzymes that cleave the prohormone precursors to produce neuropeptides. •Neuropeptides play an important role in learning, behavior, memory, and perception of stimuli and directly affect neural transmission and nerve activity. Therefore, PCs are critical for the hea ...
... •Prohormone convertases (PCs) are enzymes that cleave the prohormone precursors to produce neuropeptides. •Neuropeptides play an important role in learning, behavior, memory, and perception of stimuli and directly affect neural transmission and nerve activity. Therefore, PCs are critical for the hea ...
A E M , Feb. 2004, p. 999–1007
... Transformants were isolated, and the individual plasmids were sequenced to confirm the insertion of the desired point mutation in the fabH gene. To confirm that no other point mutations had occurred in regions that could affect the copy number or transcription of the mutant fabH genes, the correspon ...
... Transformants were isolated, and the individual plasmids were sequenced to confirm the insertion of the desired point mutation in the fabH gene. To confirm that no other point mutations had occurred in regions that could affect the copy number or transcription of the mutant fabH genes, the correspon ...
PhyPA: Phylogenetic method with pairwise sequence alignment
... is to use only PSA to reconstruct phylogenetic trees, which can only be done with distance-based methods. I compared the accuracy of this new computational approach (named PhyPA for phylogenetics by pairwise alignment) against the maximum likelihood method using MSA (the ML + MSA approach), based on ...
... is to use only PSA to reconstruct phylogenetic trees, which can only be done with distance-based methods. I compared the accuracy of this new computational approach (named PhyPA for phylogenetics by pairwise alignment) against the maximum likelihood method using MSA (the ML + MSA approach), based on ...
CURRICULUM VITAE - Cedoc - Universidade Nova de Lisboa
... proteins - in the modulation of immune responses to pathogens and in the cancer biology. Virtually every cell surface protein is glycosylated and a considerable number of proteins that regulate immune cell development and function bind glycans (i.e. are lectins). Thus, glycan modifications can chang ...
... proteins - in the modulation of immune responses to pathogens and in the cancer biology. Virtually every cell surface protein is glycosylated and a considerable number of proteins that regulate immune cell development and function bind glycans (i.e. are lectins). Thus, glycan modifications can chang ...
NEET-2017 Solved Paper
... Thalassemia and sickle cell anemia are caused due to a problem in globin molecules synthesis. Select the CORRECT statement. (1) Both are due to a qualitative defect in globin chain synthesis. (2) Both are due to a quantitative defect in globin chain synthesis. (3) Thalassemia is due to less synthesi ...
... Thalassemia and sickle cell anemia are caused due to a problem in globin molecules synthesis. Select the CORRECT statement. (1) Both are due to a qualitative defect in globin chain synthesis. (2) Both are due to a quantitative defect in globin chain synthesis. (3) Thalassemia is due to less synthesi ...
Non-Regenerative Benefits of Adult Bone Marrow Derived Stem
... mitochondrial aconitase and mitochondrial voltage-dependent anionselective channel protein-1. Thereafter, I investigated the mobilization of endogenous bone marrow stem cells and trafficking to the ischaemic myocardium by stromal cell derived factor-1 (SDF-1) /chemokine, receptor type 4 (CXCR4) sign ...
... mitochondrial aconitase and mitochondrial voltage-dependent anionselective channel protein-1. Thereafter, I investigated the mobilization of endogenous bone marrow stem cells and trafficking to the ischaemic myocardium by stromal cell derived factor-1 (SDF-1) /chemokine, receptor type 4 (CXCR4) sign ...
Improvement of Aspergillus nidulans penicillin production by
... description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or even general application of the antibiotic, many years of research passed. One of the major problems at the time was the yield of penicillin. Even nowadays increasing the yield of penicillin pr ...
... description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or even general application of the antibiotic, many years of research passed. One of the major problems at the time was the yield of penicillin. Even nowadays increasing the yield of penicillin pr ...
UNIVERSITAT ROVIRA I VIRGILI CODON USAGE ADAPTATION IN PROKARYOTIC GENOMES
... is based on the genome hypothesis (1) that assumes that codon usage and G+C content are distinct global features of each prokaryotic genome. With this method, a significant number of prokaryotic genes have been proposed as having been acquired by HGT (7, 8, 30). However, it cannot predict all acquir ...
... is based on the genome hypothesis (1) that assumes that codon usage and G+C content are distinct global features of each prokaryotic genome. With this method, a significant number of prokaryotic genes have been proposed as having been acquired by HGT (7, 8, 30). However, it cannot predict all acquir ...
αII-Spectrin interacts with Tes and EVL, two actin
... interactions, defining the multiple physiological functions of spectrins. Functions of the spectrin-based skeleton are well-defined in red blood cells where it is required for shape maintenance, for resistance to shear stress and for deformability. However, the functions of the more complex spectrin ...
... interactions, defining the multiple physiological functions of spectrins. Functions of the spectrin-based skeleton are well-defined in red blood cells where it is required for shape maintenance, for resistance to shear stress and for deformability. However, the functions of the more complex spectrin ...
Cardiac troponin I gene knockout - University of Wisconsin–Madison
... regulatory protein. Mice lacking cardiac troponin I were born healthy, with normal heart and body weight, because a fetal troponin I isoform (identical to slow skeletal troponin I) compensated for the absence of cardiac troponin I. Compensation was only temporary, however, as 15 days after birth slo ...
... regulatory protein. Mice lacking cardiac troponin I were born healthy, with normal heart and body weight, because a fetal troponin I isoform (identical to slow skeletal troponin I) compensated for the absence of cardiac troponin I. Compensation was only temporary, however, as 15 days after birth slo ...
A Truncated Laminin Chain Homologous to the B2 Chain: Structure
... This structure is stabilized by ionic interactions between the chains. Calculations of these interactions show that only heterotrimeric molecules with one A-type chain, one Bl-type chain, and one B2-type chain are favorable (20). Studies on the expression of laminin subunit genes and distribution of ...
... This structure is stabilized by ionic interactions between the chains. Calculations of these interactions show that only heterotrimeric molecules with one A-type chain, one Bl-type chain, and one B2-type chain are favorable (20). Studies on the expression of laminin subunit genes and distribution of ...
Planta
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
Pseudouridine at position 55 in tRNA controls the contents of other
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
The mitochondrial carnitine/acylcarnitine carrier: Function
... residues can be labeled with specific chemical reagents. It was found that each single Cys mutant was substantially active, demonstrating that none of the CAC Cys residues is directly involved in the transport mechanism (Indiveri et al., 2002). Interestingly, SH-blocking reagents failed to inactivate ...
... residues can be labeled with specific chemical reagents. It was found that each single Cys mutant was substantially active, demonstrating that none of the CAC Cys residues is directly involved in the transport mechanism (Indiveri et al., 2002). Interestingly, SH-blocking reagents failed to inactivate ...
Diversity and origins of anaerobic metabolism in mitochondria and
... Mitochondria are best known for their role in ATP synthesis by oxidative phosphorylation. In this pathway, pyruvate from glycolysis is imported into mitochondria where it is oxidatively decarboxylated to acetyl-CoA by pyruvate dehydrogenase (PDH) and fed into the Krebs cycle to produce NADH and FADH ...
... Mitochondria are best known for their role in ATP synthesis by oxidative phosphorylation. In this pathway, pyruvate from glycolysis is imported into mitochondria where it is oxidatively decarboxylated to acetyl-CoA by pyruvate dehydrogenase (PDH) and fed into the Krebs cycle to produce NADH and FADH ...
An introduction to informatics - Swiss
... UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if each protein of a given species is represented by exactly one amino a ...
... UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if each protein of a given species is represented by exactly one amino a ...
Review: can diet influence the selective advantage of mitochondrial
... mitohormesis [7]. Although varied, this response appears to induce a wide-ranging cytoprotective state resulting in long lasting metabolic and biochemical changes. Remarkably, rather than being harmful, these changes may increase evolutionary potential and decrease susceptibility for disease. Althou ...
... mitohormesis [7]. Although varied, this response appears to induce a wide-ranging cytoprotective state resulting in long lasting metabolic and biochemical changes. Remarkably, rather than being harmful, these changes may increase evolutionary potential and decrease susceptibility for disease. Althou ...
Ana Maria da Silva Esteves Dissertation presented to obtain
... better suited for osmoprotection. This is one more illustration of solute replacement, a strategy generally used by organisms that rely on solute accumulation for osmoregulation. The patterns of compatible solute accumulation by Pyrococcus furiosus in response to heat stress are known, but the effec ...
... better suited for osmoprotection. This is one more illustration of solute replacement, a strategy generally used by organisms that rely on solute accumulation for osmoregulation. The patterns of compatible solute accumulation by Pyrococcus furiosus in response to heat stress are known, but the effec ...
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
Multiple RNA regulatory elements mediate distinct
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
... Pattern formation in the early development of many organisms relies on localized cytoplasmic proteins, which can be prelocalized as mRNAs. The Drosophila oskar gene, required both for posterior body patterning and germ cell determination, encodes one such mRNA. Localization of oskar mRNA is an elabo ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... names of the respective proteins are given in parentheses for ease of reference.] The genomic RNA acts as a monocistronic message and is initially translated to a > 200K polyprotein which is cleaved by a series of proteolytic events to yield the mature viral products, both structural and non-structu ...
... names of the respective proteins are given in parentheses for ease of reference.] The genomic RNA acts as a monocistronic message and is initially translated to a > 200K polyprotein which is cleaved by a series of proteolytic events to yield the mature viral products, both structural and non-structu ...
Lysosomal Enzymes Are Released From Cultured Human
... TCA-soluble radioactivity, showing that proteolytic activity was released from macrophages (not shown). The level of lactate dehydrogenase activity in the control media (6.1⫾3.5% of total cellular activity) and the conditioned media from Zop-treated macrophages (6.5⫾0.3% of total cellular activity) ...
... TCA-soluble radioactivity, showing that proteolytic activity was released from macrophages (not shown). The level of lactate dehydrogenase activity in the control media (6.1⫾3.5% of total cellular activity) and the conditioned media from Zop-treated macrophages (6.5⫾0.3% of total cellular activity) ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.