Characterization of the Phosphoenolpyruvate Carboxykinase Gene
... tuberculosis (64%; SWISS-PROT P96393), M. leprae (62%; TrEMBL O06084; not shown in Figure 3), C. limicola (52%; SWISS-PROT Q08262) and T. pallidum (51%; SWISS-PROT O83159). The C. glutamicum enzyme showed also relatively high identity to several eukaryotic GTP-dependent PEP carboxykinases, e.g., fro ...
... tuberculosis (64%; SWISS-PROT P96393), M. leprae (62%; TrEMBL O06084; not shown in Figure 3), C. limicola (52%; SWISS-PROT Q08262) and T. pallidum (51%; SWISS-PROT O83159). The C. glutamicum enzyme showed also relatively high identity to several eukaryotic GTP-dependent PEP carboxykinases, e.g., fro ...
Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase
... PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by various assays to determine the treatment dosage that would result in complete cytostasis, without non-specific c ...
... PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by various assays to determine the treatment dosage that would result in complete cytostasis, without non-specific c ...
Suberin biosynthesis in O. sativa: characterisation of a cytochrome
... and stems as well as their subsurface adaptations. Roots serve as anchor for the plant body in the substrate, which is the main storage for water and nutrients. By maximising their surface area with lateral roots and root hairs, they optimised the uptake of water and solutes. The development of inte ...
... and stems as well as their subsurface adaptations. Roots serve as anchor for the plant body in the substrate, which is the main storage for water and nutrients. By maximising their surface area with lateral roots and root hairs, they optimised the uptake of water and solutes. The development of inte ...
A motif and amino acid bias bioinformatics
... PTMs on the structural, steric and electrostatic properties can result in IDPs having multiple binding ...
... PTMs on the structural, steric and electrostatic properties can result in IDPs having multiple binding ...
Cloning and sequencing of the kedarcidin biosynthetic
... ATCC 53650 cosmid library, resulting in the isolation of cosmid pBS16002. Iterations of chromosomal walking from pBS16002 using probe-2, -3, -4, and -5 afforded the four additional overlapping cosmids pBS16003, pBS16004, pBS16005, and pBS16006. Together, the five overlapping cosmids cover 135 kb of c ...
... ATCC 53650 cosmid library, resulting in the isolation of cosmid pBS16002. Iterations of chromosomal walking from pBS16002 using probe-2, -3, -4, and -5 afforded the four additional overlapping cosmids pBS16003, pBS16004, pBS16005, and pBS16006. Together, the five overlapping cosmids cover 135 kb of c ...
Regulation of Starch Synthesis in Cassava Yona Baguma
... Cassava (Manihot esculenta Crantz) is a root crop, one of the world’s most important but under-exploited staple food crops and source of income. It is a high starch producer with levels between 73.7 and 84.9% of its total storage root dry weight. Increasingly, there is a need for diverse novel starc ...
... Cassava (Manihot esculenta Crantz) is a root crop, one of the world’s most important but under-exploited staple food crops and source of income. It is a high starch producer with levels between 73.7 and 84.9% of its total storage root dry weight. Increasingly, there is a need for diverse novel starc ...
Intracellular Localization of Interleukin-6 in
... blood eosinophils express mRNA for IL-6.6 In addition, IL-6– positive eosinophils have been detected in blood from normal donors, suggesting that IL-6 may be constitutively synthesized and stored in unstimulated eosinophils.7 The site of storage of IL-6 in eosinophils could not be determined by immu ...
... blood eosinophils express mRNA for IL-6.6 In addition, IL-6– positive eosinophils have been detected in blood from normal donors, suggesting that IL-6 may be constitutively synthesized and stored in unstimulated eosinophils.7 The site of storage of IL-6 in eosinophils could not be determined by immu ...
Oxidative stress and protection against reactive oxygen
... NADPH oxidase: Production of superoxide anion and H2O2 via NADPH oxidase has been described on a rabbit blastocyst surface (Manes and Lai, 1995). Also, incubation of 2-cell mouse embryos with an inhibitor of NADPH oxidase induces a concentrationdependent reduction in H2O2 production (Nasr-Esfahani a ...
... NADPH oxidase: Production of superoxide anion and H2O2 via NADPH oxidase has been described on a rabbit blastocyst surface (Manes and Lai, 1995). Also, incubation of 2-cell mouse embryos with an inhibitor of NADPH oxidase induces a concentrationdependent reduction in H2O2 production (Nasr-Esfahani a ...
Metabolism and function of bile acids
... derivatives. In other vertebrate species, the hydroxylation of the side chain does not lead to its removal and the products of the biosynthetic pathway are referred to as bile alcohols. Invertebrate species do not synthesize sterol bile acids. Over the last few years, much information has been gaine ...
... derivatives. In other vertebrate species, the hydroxylation of the side chain does not lead to its removal and the products of the biosynthetic pathway are referred to as bile alcohols. Invertebrate species do not synthesize sterol bile acids. Over the last few years, much information has been gaine ...
MITOCHONDRIAL DNA REPLICATION IN PRE
... 2.1.3 Cryopreservation of cultured cells.......................................................................51 2.1.4 Spontaneous ESC differentiation and embryoid body production ......................52 2.1.5 Directed production of embryoid bodies and ESC differentiation ......................52 ...
... 2.1.3 Cryopreservation of cultured cells.......................................................................51 2.1.4 Spontaneous ESC differentiation and embryoid body production ......................52 2.1.5 Directed production of embryoid bodies and ESC differentiation ......................52 ...
The characterization of inner core protein VP6 of African Horsesickness Virus
... CHAPTER 1: LITERATURE REVIEW 1.1 INTRODUCTION In 1898 researchers identified infectious agents that were smaller than the smallest known bacteria. Viruses are obligate intracellular parasites that use the machinery of the host cell to replicate. They consist of a DNA or RNA genome surrounded by a pr ...
... CHAPTER 1: LITERATURE REVIEW 1.1 INTRODUCTION In 1898 researchers identified infectious agents that were smaller than the smallest known bacteria. Viruses are obligate intracellular parasites that use the machinery of the host cell to replicate. They consist of a DNA or RNA genome surrounded by a pr ...
ABA overlysensitive5 (ABO5), encoding a pentatricopeptide repeat
... had the same ABA-insensitive phenotype as the abi single mutants (Figure 1h, right lane). When the response of root growth to ABA was measured, abo5 abi1-1 and abo5 abi2-1 were similar to abi1-1 and abi2-1 in that they were insensitive to ABA, but abo5 abi3-1, abo5 abi4-1 and abo5 abi5 differed from ...
... had the same ABA-insensitive phenotype as the abi single mutants (Figure 1h, right lane). When the response of root growth to ABA was measured, abo5 abi1-1 and abo5 abi2-1 were similar to abi1-1 and abi2-1 in that they were insensitive to ABA, but abo5 abi3-1, abo5 abi4-1 and abo5 abi5 differed from ...
Identity elements in tRNA-mediated transcription
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
Interactions of TCA cycle enzymes and of the CcpA
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
Noba Vital range - Noba Vital Plus, Noba Vital
... developed Noba Vital Pure on the basis of a unique, fatty acid complex formula consisting of medium-chain fatty acids (C8-C10-C12). Scientific research performed by Noba has shown that Noba Vital Plus has a positive effect on the villus/crypt ratio. This results in the growth of a vital intestinal e ...
... developed Noba Vital Pure on the basis of a unique, fatty acid complex formula consisting of medium-chain fatty acids (C8-C10-C12). Scientific research performed by Noba has shown that Noba Vital Plus has a positive effect on the villus/crypt ratio. This results in the growth of a vital intestinal e ...
Nature inspired platforms for production of acetyl
... been reviewed previously11,12,13 and will not be discussed. We then discuss advantages of pathway engineering in S. cerevisiae, review S. cerevisiae central metabolism and native acetylCoA generation, discuss recent S. cerevisiae central metabolism engineering efforts, introduce ...
... been reviewed previously11,12,13 and will not be discussed. We then discuss advantages of pathway engineering in S. cerevisiae, review S. cerevisiae central metabolism and native acetylCoA generation, discuss recent S. cerevisiae central metabolism engineering efforts, introduce ...
A Comparative Study - Carlos Ibanez Lab @ KI
... Production and quantitation of recombinant NGF Genomic fragments containing the preproNGF coding sequences from the human (see Materials and Methods), rat (Whittemore et al., 1988), and chicken (Ebendal et al., 1986) NGF genes were subcloned in the expression plasmid vector pXM (Yang et al., 1986) a ...
... Production and quantitation of recombinant NGF Genomic fragments containing the preproNGF coding sequences from the human (see Materials and Methods), rat (Whittemore et al., 1988), and chicken (Ebendal et al., 1986) NGF genes were subcloned in the expression plasmid vector pXM (Yang et al., 1986) a ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
... can be found in the Pfam database (Table S1)22. The majority of these proteins are found in bacterial species but a small number are also present in archaea within the Euryarchaeota ...
... can be found in the Pfam database (Table S1)22. The majority of these proteins are found in bacterial species but a small number are also present in archaea within the Euryarchaeota ...
Molecular Characterization of Visual Pigments in
... RhC was performed except that the primer was TlOpC-R. The product of 5# RACE for RhB was generated with ds cDNA, TlOpB-R, and UPM, and PCR conditions comprised 5 cycles of 5 s at 94 °C and 2 min at 72 °C; 5 cycles of 5 s at 94 °C, 10 s at 70 °C, and 2 min at 72 °C; and 25 cycles of 5 s at 94 °C, 10 ...
... RhC was performed except that the primer was TlOpC-R. The product of 5# RACE for RhB was generated with ds cDNA, TlOpB-R, and UPM, and PCR conditions comprised 5 cycles of 5 s at 94 °C and 2 min at 72 °C; 5 cycles of 5 s at 94 °C, 10 s at 70 °C, and 2 min at 72 °C; and 25 cycles of 5 s at 94 °C, 10 ...
Cloning, Sequencing, and Characterization of Luciola italica
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
... comparisons among firefly species can be used to determine important structure-function similarities and differences among their luciferase enzymes. Amino acids shared between species’ sequences are likely to serve an important role in the catalysis of this reaction and identifying them will lead to ...
- BioMedSearch
... effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase whose use can be limited by feedback inhibition. Overexpression of feedback insensiti ...
... effective anti-influenza agent (see [16]). E. coli, overexpressing PEPS, are widely used in the biosynthesis of shikimic acid as is over-expression of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase whose use can be limited by feedback inhibition. Overexpression of feedback insensiti ...
Regulation of Cytochrome bd Expression in Mycobacterium
... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
Characterization of cytochrome P450
... Bank for Medicinal and Aromatic Plants (NGBMAP) maintained at the Central Institute of Medicinal and Aromatic Plants in India, were grown in the fields during February to August. The plants were also grown in pots and maintained in the glass house. Leaf material was collected from 5 month old plants ...
... Bank for Medicinal and Aromatic Plants (NGBMAP) maintained at the Central Institute of Medicinal and Aromatic Plants in India, were grown in the fields during February to August. The plants were also grown in pots and maintained in the glass house. Leaf material was collected from 5 month old plants ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... which is missing exon 5, QTRTD1 variant_1 (Qv1), which contains all nine exons and QTRTD1 variant_2 (Qv2), which in addition to containing all nine exons has retained one third of intron 6, exploiting a later downstream cryptic donor site to engage with exon 7. The three QTRTD1 splice variants were ...
... which is missing exon 5, QTRTD1 variant_1 (Qv1), which contains all nine exons and QTRTD1 variant_2 (Qv2), which in addition to containing all nine exons has retained one third of intron 6, exploiting a later downstream cryptic donor site to engage with exon 7. The three QTRTD1 splice variants were ...
biolsci.org - International Journal of Biological Sciences
... KHG20612, Figure 3a) and Bacillus anthracis are documented [9, 10, 25, 26]. ...
... KHG20612, Figure 3a) and Bacillus anthracis are documented [9, 10, 25, 26]. ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.