Lysosomal Enzymes Are Released From Cultured Human
... TCA-soluble radioactivity, showing that proteolytic activity was released from macrophages (not shown). The level of lactate dehydrogenase activity in the control media (6.1⫾3.5% of total cellular activity) and the conditioned media from Zop-treated macrophages (6.5⫾0.3% of total cellular activity) ...
... TCA-soluble radioactivity, showing that proteolytic activity was released from macrophages (not shown). The level of lactate dehydrogenase activity in the control media (6.1⫾3.5% of total cellular activity) and the conditioned media from Zop-treated macrophages (6.5⫾0.3% of total cellular activity) ...
Cra-mediated regulation of Escherichia coli adenylate cyclase
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... the non-reduced molecule in excited state), called reactive oxygen species (ROS), along with reactive nitrogen species (RNS) derived from nitric oxide, are molecules of „two faces“, since they can be either beneficial or harmful to organisms, all in the dependence of their equilibrium (19,177,209). ...
... the non-reduced molecule in excited state), called reactive oxygen species (ROS), along with reactive nitrogen species (RNS) derived from nitric oxide, are molecules of „two faces“, since they can be either beneficial or harmful to organisms, all in the dependence of their equilibrium (19,177,209). ...
GC-content of synonymous codons profoundly influences amino
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
... could be related to the different amino acid usage patterns in organisms. In order to address this question, we focused on the relationship between GCsyn, regional GC-content, and amino acid usage in different genomes, hypothesizing that the amino acids with high GCsyn could be used more frequently ...
Journal of Microbiology and Biotechnology
... and rhizoplane of various plant species [11, 13, 26]. The beneficial effects of P. polymyxa on plant growth, including nitrogen fixation, plant-disease suppression, soil-phosphorous solubilization, increase of soil porosity, and production of antibiotics, hydrolytic enzymes, and phytohormones, have ...
... and rhizoplane of various plant species [11, 13, 26]. The beneficial effects of P. polymyxa on plant growth, including nitrogen fixation, plant-disease suppression, soil-phosphorous solubilization, increase of soil porosity, and production of antibiotics, hydrolytic enzymes, and phytohormones, have ...
attached paper highlights
... PGC-1a in myotubes strongly induces the expression of downstream transcription factors such as NRFs and Tfam.17 Unlike NRFs or Tfam, however, PGC-1a levels correlate with cardiac and skeletal muscle oxidative capacity, suggesting that it plays a major role in setting mitochondrial content.18 PGC-1a ...
... PGC-1a in myotubes strongly induces the expression of downstream transcription factors such as NRFs and Tfam.17 Unlike NRFs or Tfam, however, PGC-1a levels correlate with cardiac and skeletal muscle oxidative capacity, suggesting that it plays a major role in setting mitochondrial content.18 PGC-1a ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
... RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral si ...
Hypoxia in skeletal muscles: from physiology to gene expression
... users including authors of articles to copy and redistribute the material in any medium or format, in addition to remix, transform, and build upon the material for any purpose, even commercially, as long as the author and original source are properly cited or credited. ...
... users including authors of articles to copy and redistribute the material in any medium or format, in addition to remix, transform, and build upon the material for any purpose, even commercially, as long as the author and original source are properly cited or credited. ...
Cells: building blocks of living organisms
... Prokarya: the cells of those organisms, prokaryotes, do not have a nucleus. Representative organisms are cyanobacteria (blue-green algae) and Escherichia coli (a common bacteria) Eukarya: the cells of those organisms, eukaryotes, all have a nucleus. Representative organisms are Trypanosoma brucei (u ...
... Prokarya: the cells of those organisms, prokaryotes, do not have a nucleus. Representative organisms are cyanobacteria (blue-green algae) and Escherichia coli (a common bacteria) Eukarya: the cells of those organisms, eukaryotes, all have a nucleus. Representative organisms are Trypanosoma brucei (u ...
BACILLUS SPHAERICUS TOXINS: Molecular Biology and Mode of
... although the activity is weaker than that of crystals containing both proteins (58). In contrast, P51 alone is not toxic, but its presence enhances the larvicidal activity of P42, suggesting synergy between the polypeptides (18, 40, 58,61). In vitro assays on mosquito cell lines also revealed that ...
... although the activity is weaker than that of crystals containing both proteins (58). In contrast, P51 alone is not toxic, but its presence enhances the larvicidal activity of P42, suggesting synergy between the polypeptides (18, 40, 58,61). In vitro assays on mosquito cell lines also revealed that ...
PDF - Geoff McFadden`s Lab
... delayed-death effect and presents a mystery, the solution to which requires that we determine the precise function of the organelle. Presumably, whatever the apicoplast provides for the parasite is crucial for a viable infection process. This could be a component of the PARASITOPHOROUS VACUOLE, whic ...
... delayed-death effect and presents a mystery, the solution to which requires that we determine the precise function of the organelle. Presumably, whatever the apicoplast provides for the parasite is crucial for a viable infection process. This could be a component of the PARASITOPHOROUS VACUOLE, whic ...
AMINO ACID SEQUENCE ANALYSIS OF HUMAN INTERLEUKIN 1
... Progress has recently been made in defining the nucleic acid sequences of messenger RNA coding for IL-1. Lomedico, et al. (12) have published a cloned cDNA coding for P388Dl-derived murine IL-1. This nucleotide sequence was validated by the sequencing of two peptides derived by CNBr cleavage of puri ...
... Progress has recently been made in defining the nucleic acid sequences of messenger RNA coding for IL-1. Lomedico, et al. (12) have published a cloned cDNA coding for P388Dl-derived murine IL-1. This nucleotide sequence was validated by the sequencing of two peptides derived by CNBr cleavage of puri ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... (Horner, Hirt, and Embley 1999), (2) some extant eukaryotes should possess genes for both PFO and PDH, (3) ATP-producing organelles with energy metabolism intermediate to that in typical hydrogenosomes and mitochondria should be found, and (4) these should harbor biochemical relics of a facultativel ...
... (Horner, Hirt, and Embley 1999), (2) some extant eukaryotes should possess genes for both PFO and PDH, (3) ATP-producing organelles with energy metabolism intermediate to that in typical hydrogenosomes and mitochondria should be found, and (4) these should harbor biochemical relics of a facultativel ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
Potassium starvation responses in yeast highlight novel potassium-related functions
... cells to the proper redox state. Yeast cells growing on ammonium as nitrogen source but lacking potassium accumulate large amounts of intracellular ammonium, which is transported through Trk1 taking advantage of the chemical similarity of both cations. Ammonium accumulation activates the retrograde ...
... cells to the proper redox state. Yeast cells growing on ammonium as nitrogen source but lacking potassium accumulate large amounts of intracellular ammonium, which is transported through Trk1 taking advantage of the chemical similarity of both cations. Ammonium accumulation activates the retrograde ...
Rabbit genome editing with zinc finger nucleases
... modi?ed rabbit is derived may comprise an ortholog corre sponding to the functional human disease or trait-related pro tein. In this case, the orthologous sequence in the “human ized” rabbit is inactivated such that no functional protein is made and the “humanized” rabbit comprises at least one ...
... modi?ed rabbit is derived may comprise an ortholog corre sponding to the functional human disease or trait-related pro tein. In this case, the orthologous sequence in the “human ized” rabbit is inactivated such that no functional protein is made and the “humanized” rabbit comprises at least one ...
Mutations to nonsense codons in human genetic
... suppressors may cause readthrough of natural stop codons. In addition, the presence of nonsense mutations can lead to the aberrant splicing of introns, and to reduced levels of complete mRNA (4,5). As these events are both nuclear in location, they are probably beyond the reach of cytoplasmic suppre ...
... suppressors may cause readthrough of natural stop codons. In addition, the presence of nonsense mutations can lead to the aberrant splicing of introns, and to reduced levels of complete mRNA (4,5). As these events are both nuclear in location, they are probably beyond the reach of cytoplasmic suppre ...
Formation of Monoterpenes in Antirrhinum majus
... developmental stages as well as fertilized and unfertilized carpels. This search revealed three different GGPPS-like contigs. Of these, we determined experimentally that one was not expressed in petals (data not shown), and a second sequence was expressed in petals but had no enzymatic activity in E ...
... developmental stages as well as fertilized and unfertilized carpels. This search revealed three different GGPPS-like contigs. Of these, we determined experimentally that one was not expressed in petals (data not shown), and a second sequence was expressed in petals but had no enzymatic activity in E ...
Full-Text PDF
... to onset of disease. Genome wide association studies located two single nucleotide polymorphisms downstream of cerebellin 2 (CBLN2) that were associated with a two-fold increased risk of PAH [12]. Mutations in KCNA5, the potassium channel voltage gated shaker-related subfamily A, member 5, which is ...
... to onset of disease. Genome wide association studies located two single nucleotide polymorphisms downstream of cerebellin 2 (CBLN2) that were associated with a two-fold increased risk of PAH [12]. Mutations in KCNA5, the potassium channel voltage gated shaker-related subfamily A, member 5, which is ...
AMP-activated protein kinase phosphorylates transcription factors of
... competition assay was performed. For the first reactions, AMPK and PKA were allowed (separately) to phosphorylate CREB1 for 60 min in the presence of 0.2 mM ATP (unlabeled) and [␥-32P]ATP (labeled ATP), as described above. In the next reactions, AMPK was allowed to phosphorylate CREB1 for 30 min usi ...
... competition assay was performed. For the first reactions, AMPK and PKA were allowed (separately) to phosphorylate CREB1 for 60 min in the presence of 0.2 mM ATP (unlabeled) and [␥-32P]ATP (labeled ATP), as described above. In the next reactions, AMPK was allowed to phosphorylate CREB1 for 30 min usi ...
9th Grade Physical Science Course Syllabus
... Solve word problems involving the frequency of genes in a population. Define the term microevolution. Define genetic drift. Explain how it contributes to microevolution. Define and explain bottleneck effect and founder effect. Describe how gene flow, mutation, nonrandom mating and natural selection ...
... Solve word problems involving the frequency of genes in a population. Define the term microevolution. Define genetic drift. Explain how it contributes to microevolution. Define and explain bottleneck effect and founder effect. Describe how gene flow, mutation, nonrandom mating and natural selection ...
FIST/HIPK3: a Fas/FADD-interacting Serine/Threonine Kinase that
... was provided by S. Hollenberg (Vollum Institute, Portland, Oregon). The two-hybrid screen and the generation of cytoplasmic mutants of Fas were previously described (16). Cloning of FIST/HIPK3 cDNA. A cDNA clone for mFIST/ HIPK3 was isolated from a mouse testis cDNA library (Stratagene) using a 32P- ...
... was provided by S. Hollenberg (Vollum Institute, Portland, Oregon). The two-hybrid screen and the generation of cytoplasmic mutants of Fas were previously described (16). Cloning of FIST/HIPK3 cDNA. A cDNA clone for mFIST/ HIPK3 was isolated from a mouse testis cDNA library (Stratagene) using a 32P- ...
DEVELOPMENT AND CHARACTERIZATION OF A TRANSGENIC
... And last but by no means least I would like to thank my wife, Youzhen Wang, for her love, faith, and support. ...
... And last but by no means least I would like to thank my wife, Youzhen Wang, for her love, faith, and support. ...
Full-Text PDF
... In contrast to TIGAR, PFKFB4, the testis isoenzyme of PFK/FBPases, is downregulated by p53. It has been shown that p53 binds to the PFKFB4 promoter, leading to transcriptional repression via histone deacetylases [48]. PFKFB4 was previously shown to be essential for the survival of prostate cancer ce ...
... In contrast to TIGAR, PFKFB4, the testis isoenzyme of PFK/FBPases, is downregulated by p53. It has been shown that p53 binds to the PFKFB4 promoter, leading to transcriptional repression via histone deacetylases [48]. PFKFB4 was previously shown to be essential for the survival of prostate cancer ce ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.