Proteolytic processing in the secretory pathway of Aspergillus niger
... lethal for immunocompromised patients, but other non-pathogenic fungi are used for the production of pharmaceutical products. Fungi are the most important group of plant pathogens, however, mycorrhizal fungi that grow interdependently with plant roots are critical for nutrient uptake by plants. In a ...
... lethal for immunocompromised patients, but other non-pathogenic fungi are used for the production of pharmaceutical products. Fungi are the most important group of plant pathogens, however, mycorrhizal fungi that grow interdependently with plant roots are critical for nutrient uptake by plants. In a ...
Nucleic Acids Research
... Ach5 tmz' locus shows over 88% homology in the 240 bases 5' to the translational initiation codon and over 91% homology in the coding sequences. The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of c ...
... Ach5 tmz' locus shows over 88% homology in the 240 bases 5' to the translational initiation codon and over 91% homology in the coding sequences. The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of c ...
Enzyme Mechanisms - Illinois Institute of Technology
... complete sequencing efforts Generally: amplification and expression give us access to and control of biochemical systems that otherwise have to be isolated in their original setting These methods enable controlled experiments on complex systems ...
... complete sequencing efforts Generally: amplification and expression give us access to and control of biochemical systems that otherwise have to be isolated in their original setting These methods enable controlled experiments on complex systems ...
DANIELE GHEZZI Matr. N°. 708361 Identification and
... but, despite several studies, further cases of paternal transmission have never been described. The dogma of the maternal inheritance of the mtDNA remains true in practice for genetic counselling and for evolutionary studies. Each human cell has thousands of mitochondria and within each single mitoc ...
... but, despite several studies, further cases of paternal transmission have never been described. The dogma of the maternal inheritance of the mtDNA remains true in practice for genetic counselling and for evolutionary studies. Each human cell has thousands of mitochondria and within each single mitoc ...
Eater, a Transmembrane Protein Mediating
... in vitro transcribed and translated an eater cDNA derived from S2 cells (clone SD22390), as well as the open reading frame of eater fused to a C-terminal histidine tag. In both cases, we obtained a single specific translation product in the 120 kDa range (Figures 2A and 2B). Truncation of the cDNAs ...
... in vitro transcribed and translated an eater cDNA derived from S2 cells (clone SD22390), as well as the open reading frame of eater fused to a C-terminal histidine tag. In both cases, we obtained a single specific translation product in the 120 kDa range (Figures 2A and 2B). Truncation of the cDNAs ...
Threading-based Protein Structure Prediction
... • DNA is usually tightly wound around histone proteins and forms a chromosome • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
... • DNA is usually tightly wound around histone proteins and forms a chromosome • The total info stored in all chromosomes constitutes a genome • In most multi-cell organisms, every cell contains the same complete set of chromosomes – May have some small different due to mutation ...
Early bioenergetic evolution
... temperature often exceeding 3008C. Such ‘black smokers’ are not only very hot, but they are also short-lived, lasting in the order of decades. In contrast, off-ridge vents of the kind exemplified by Lost City [13,17] are situated several kilometres away from the spreading zone; hence the water circu ...
... temperature often exceeding 3008C. Such ‘black smokers’ are not only very hot, but they are also short-lived, lasting in the order of decades. In contrast, off-ridge vents of the kind exemplified by Lost City [13,17] are situated several kilometres away from the spreading zone; hence the water circu ...
Moore BA, Gonzalez Aviles GD, Larkins CE, Hillman MJ, Caspary T. Mamm Genome. 2010 Aug;21(7-8):350-60. Mitochondrial retention of Opa1 is required for mouse embryogenesis
... (mtDNA). When these dynamics are lacking, mitochondria develop abnormal morphologies (reviewed in Detmer and Chan 2007). The biological significance of these dynamics is clear, since disrupting them leads to alterations in apoptosis, calcium buffering, and ATP production (Duchen 2000; Kroemer and Re ...
... (mtDNA). When these dynamics are lacking, mitochondria develop abnormal morphologies (reviewed in Detmer and Chan 2007). The biological significance of these dynamics is clear, since disrupting them leads to alterations in apoptosis, calcium buffering, and ATP production (Duchen 2000; Kroemer and Re ...
Are You suprised ?
... The biochemical comparison of proteins is a technique used to determine evolutionary relationships among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids i ...
... The biochemical comparison of proteins is a technique used to determine evolutionary relationships among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids i ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... letters to nature cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocy ...
... letters to nature cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocy ...
Get PDF - Wiley Online Library
... The poly(A) tail is bound by the poly(A)-binding protein (PABP), which is necessary for efficient translation (for reviews, see Jacobson, 1996; Sachs et al., 1997). In yeast, eIF4G was found to associate with PABP (Tarun and Sachs, 1996). The PABP-binding site was mapped to an N-terminal region prox ...
... The poly(A) tail is bound by the poly(A)-binding protein (PABP), which is necessary for efficient translation (for reviews, see Jacobson, 1996; Sachs et al., 1997). In yeast, eIF4G was found to associate with PABP (Tarun and Sachs, 1996). The PABP-binding site was mapped to an N-terminal region prox ...
11 Cytochrome P450 and the Metabolism and Bioactivation of
... synthase and the lipoxygenases are typical dioxygenases that catalyze substrate carbon activation instead of oxygen activation. It is apparent from the literature that the P450 gene superfamily of hemoproteins is, as a group, one of the most intensively studied enzyme systems and yet our knowledge o ...
... synthase and the lipoxygenases are typical dioxygenases that catalyze substrate carbon activation instead of oxygen activation. It is apparent from the literature that the P450 gene superfamily of hemoproteins is, as a group, one of the most intensively studied enzyme systems and yet our knowledge o ...
Altered pathogenicity, immunogenicity, tissue tropism and 3`
... the 3'-7 kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes. Keywords: Avian infectious bronchitis coronavirus; Path ...
... the 3'-7 kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes. Keywords: Avian infectious bronchitis coronavirus; Path ...
Evidence for Evolution
... 4. At which stage is it the most difficult to tell the embryos apart? (I, II, or III) 5. Describe at least one similarity in the structure of these embryos 6. How do these similarities support the Theory of Evolution? ...
... 4. At which stage is it the most difficult to tell the embryos apart? (I, II, or III) 5. Describe at least one similarity in the structure of these embryos 6. How do these similarities support the Theory of Evolution? ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
... the [4Fe-4S] cluster (residues 1-50), central allosteric domain contacting RNA polymerase, and C-terminal regulatory domain incorporating the H–T–H region [43]. In P. aeruginosa and some other Pseudomonas species the Anr protein (anaerobic regulation of anaerobic deiminase and NRase) was identified ...
... the [4Fe-4S] cluster (residues 1-50), central allosteric domain contacting RNA polymerase, and C-terminal regulatory domain incorporating the H–T–H region [43]. In P. aeruginosa and some other Pseudomonas species the Anr protein (anaerobic regulation of anaerobic deiminase and NRase) was identified ...
Protein Feature Identification
... • A publicly available web server that performs automated annotation of bacterial genomes given only the gene sequence of a chromosome or plasmid • Takes about 24 hrs for an average genome (4 megabases) • Output includes images and annotation text (about 70 fields for each gene) Lecture 2.5 ...
... • A publicly available web server that performs automated annotation of bacterial genomes given only the gene sequence of a chromosome or plasmid • Takes about 24 hrs for an average genome (4 megabases) • Output includes images and annotation text (about 70 fields for each gene) Lecture 2.5 ...
Proteins of Human Milk. I. Identification of Major Components
... which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules of human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (3 ...
... which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules of human milk (28). Casein micelles and soluble (whey) proteins are collected in to secretory vesicles fo r exocytosis (3 ...
The phosphopantetheinyl transferases
... other central biosynthetic pathways in both primary and specialized metabolism. The essential enzymatic role of PPTases in general fatty acid biosynthesis was recognized in the groundbreaking work of Vagelos and Elovson.3 Since then, many other PPTases have been discovered to play the same role in a ...
... other central biosynthetic pathways in both primary and specialized metabolism. The essential enzymatic role of PPTases in general fatty acid biosynthesis was recognized in the groundbreaking work of Vagelos and Elovson.3 Since then, many other PPTases have been discovered to play the same role in a ...
Molecular analysis and biological implications of STAT3 signal
... STAT3 ser727 phosphorylation. PKCδ is the end-point kinase of the Rac-MEKK-SEK-1 signal transduction cascade and phosphorylates STAT3 on the ser727 residue in response to IL-6. It is plausible that JNK-1 and PKCδ are anchored in different signal transduction protein complexes and that these complexe ...
... STAT3 ser727 phosphorylation. PKCδ is the end-point kinase of the Rac-MEKK-SEK-1 signal transduction cascade and phosphorylates STAT3 on the ser727 residue in response to IL-6. It is plausible that JNK-1 and PKCδ are anchored in different signal transduction protein complexes and that these complexe ...
Role of the non-respiratory pathways in the utilization of molecular
... (AOX), located on the matrix side of the inner mitochondrial membrane and encoded by a member of the AOX gene family.179 The constitutive or inducible AOX bypasses the cytochrome chain by directly transferring electrons from ubiquinol to oxygen. The alternative respiration is also called ‘ubiquinol ...
... (AOX), located on the matrix side of the inner mitochondrial membrane and encoded by a member of the AOX gene family.179 The constitutive or inducible AOX bypasses the cytochrome chain by directly transferring electrons from ubiquinol to oxygen. The alternative respiration is also called ‘ubiquinol ...
Are You suprised ?
... The biochemical comparison of proteins is a technique used to determine evolutionary relationships among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids i ...
... The biochemical comparison of proteins is a technique used to determine evolutionary relationships among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids i ...
pdf
... themselves are 100% identical. The plasmid comprises mostly hypothetical and conserved hypothetical proteins. A transposase (Noc_A0021), phage integrase (Noc_A0015), and a small number of other phagerelated genes along with restriction modification systems are also found on this replicon. A possible ...
... themselves are 100% identical. The plasmid comprises mostly hypothetical and conserved hypothetical proteins. A transposase (Noc_A0021), phage integrase (Noc_A0015), and a small number of other phagerelated genes along with restriction modification systems are also found on this replicon. A possible ...
Evidence from glycine transfer RNA of a frozen accident at the dawn
... form. Each form could be aminoacylated with glycine by an RNA predecessor of a modern aminoacyl tRNA synthetase (Figure 1, bottom left and middle). The ligated duplex glycine RNA evolved into the modern glycine tRNA, but in this proposal has also experienced expanded evolution by duplication and mut ...
... form. Each form could be aminoacylated with glycine by an RNA predecessor of a modern aminoacyl tRNA synthetase (Figure 1, bottom left and middle). The ligated duplex glycine RNA evolved into the modern glycine tRNA, but in this proposal has also experienced expanded evolution by duplication and mut ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic ace ...
... A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic ace ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.