Identification of the Gene Encoding the Tryptophan Synthase ß
... mL) were preincubated for 10 min at 30°C with all components except indole. At several time points after the addition of indole, 1-mL aliquots were removed and the reaction stopped by mixing with 4 mL of toluene. The indole content of each toluene phase was measured colorimetrically using Ehrlich’s ...
... mL) were preincubated for 10 min at 30°C with all components except indole. At several time points after the addition of indole, 1-mL aliquots were removed and the reaction stopped by mixing with 4 mL of toluene. The indole content of each toluene phase was measured colorimetrically using Ehrlich’s ...
Molecular cloning, characterization and gene expression of an
... Keywords: Catalase Macrobrachium rosenbergii IHHNV Gene expression Protein characterization ...
... Keywords: Catalase Macrobrachium rosenbergii IHHNV Gene expression Protein characterization ...
Prying Into Prions - Colorado Parks and Wildlife
... moose. It belongs to a family of diseases known as transmissible spongiform encephalopathies or prion diseases. The disease attacks the brains of infected deer, elk and moose, causing the animals to become emaciated, display abnormal behavior and lose coordination, and eventually die. Chronic Wastin ...
... moose. It belongs to a family of diseases known as transmissible spongiform encephalopathies or prion diseases. The disease attacks the brains of infected deer, elk and moose, causing the animals to become emaciated, display abnormal behavior and lose coordination, and eventually die. Chronic Wastin ...
Green Fluorescent Protein
... about protein interactions, timing of cell cycle events, and much more. ...
... about protein interactions, timing of cell cycle events, and much more. ...
A1060 GM Corn Line 5307 AppR SD1 Safety Assess
... analytes were noted, but these occurred randomly across trial sites. Differences such as these simply reflect the fact that a large number of tests were performed, and approximately 5% of these would be expected to show statistically significant differences by chance alone. ...
... analytes were noted, but these occurred randomly across trial sites. Differences such as these simply reflect the fact that a large number of tests were performed, and approximately 5% of these would be expected to show statistically significant differences by chance alone. ...
A genetically encoded fluorescent tRNA is active in live
... need for ex vivo technologies. We show here that the fusion of a large RNA aptamer with one of the largest tRNAs, despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quant ...
... need for ex vivo technologies. We show here that the fusion of a large RNA aptamer with one of the largest tRNAs, despite each having a well-defined tertiary structure, enables the tRNA to perform live-cell protein synthesis in Escherichia coli. This tRNA–aptamer fusion can be switched on with quant ...
4. Characterisation of novel proteins
... analytes were noted, but these occurred randomly across trial sites. Differences such as these simply reflect the fact that a large number of tests were performed, and approximately ...
... analytes were noted, but these occurred randomly across trial sites. Differences such as these simply reflect the fact that a large number of tests were performed, and approximately ...
Cloning and sequencing of a gene encoding acidophilic amylase
... Purification of the enzyme and determination of peptide sequences. Plate cultures of B. acidocaldarius were suspended in 0.5 M-Na acetate (PH 50), incubated for 30 min at 4 "C, and the cells were removed by centrifugation at 8000 g for 20 min. The supernatant was centrifuged again at 40000 g for 30 ...
... Purification of the enzyme and determination of peptide sequences. Plate cultures of B. acidocaldarius were suspended in 0.5 M-Na acetate (PH 50), incubated for 30 min at 4 "C, and the cells were removed by centrifugation at 8000 g for 20 min. The supernatant was centrifuged again at 40000 g for 30 ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
... more common in the elderly and those living in rural areas [4,7,9,11]. In addition, C. jejuni is most commonly found in poultry and ruminants, whereas C. coli colonizes pigs more frequently. Nevertheless, C. coli is also found in poultry, and it has been suggested that the populations circulating in ...
... more common in the elderly and those living in rural areas [4,7,9,11]. In addition, C. jejuni is most commonly found in poultry and ruminants, whereas C. coli colonizes pigs more frequently. Nevertheless, C. coli is also found in poultry, and it has been suggested that the populations circulating in ...
Research Associate, Dept
... parvum enzymes may be able to elongate, from medium-chain fatty acids, saturated and unsaturated long-chain fatty acids and polyketides using acetyl-CoA as an elongation unit. Although the exact function is not known for either PKS1 or FAS1, one or both may represent attractive drug targets which sh ...
... parvum enzymes may be able to elongate, from medium-chain fatty acids, saturated and unsaturated long-chain fatty acids and polyketides using acetyl-CoA as an elongation unit. Although the exact function is not known for either PKS1 or FAS1, one or both may represent attractive drug targets which sh ...
Deficiency in plastidic glutamine synthetase alters proline
... to that of WT during the first 5 d of water deprivation (Fig. 1a). Although the plants remained fully rehydratable after 5 d of treatment, indicating that drought stress was still reversible, longer periods of water deprivation (6 and 7 d) resulted in a large difference in the rehydration ability of ...
... to that of WT during the first 5 d of water deprivation (Fig. 1a). Although the plants remained fully rehydratable after 5 d of treatment, indicating that drought stress was still reversible, longer periods of water deprivation (6 and 7 d) resulted in a large difference in the rehydration ability of ...
pdf file - College of Computer, Mathematical, and Natural Sciences
... with two 68 amino acid repeats, dubbed the AP2 domain, that is predicted to perform functions of protein-protein dimerization and DNA binding (Jofuku et al., 1994; Riechmann and Meyerowitz, 1998; Nole-Wilson and Krizek, 2000). LUG encodes a glutamine-rich protein with seven WD repeats and was predic ...
... with two 68 amino acid repeats, dubbed the AP2 domain, that is predicted to perform functions of protein-protein dimerization and DNA binding (Jofuku et al., 1994; Riechmann and Meyerowitz, 1998; Nole-Wilson and Krizek, 2000). LUG encodes a glutamine-rich protein with seven WD repeats and was predic ...
The Role of Nucleoside Diphosphate Kinase in Plant Mitochondria
... organisms from bacteria to human. It was discovered that the genes nm23 and awd, which encode NDPKs are involved in tumour metastasis and Drosophila development, respectively. Thus, NDPK isoforms have been suggested to have specific regulatory functions in addition to their catalytic activity. Plant ...
... organisms from bacteria to human. It was discovered that the genes nm23 and awd, which encode NDPKs are involved in tumour metastasis and Drosophila development, respectively. Thus, NDPK isoforms have been suggested to have specific regulatory functions in addition to their catalytic activity. Plant ...
Subroto Chatterjee CV
... 81. Martin S, Williams N, and Chatterjee S. Lactosylceramide is required in apoptosis induced by Neutral sphingomyelinase. Glycoconjugate J. 23;147-157 2006. 82. Chatterjee,S Kolmakova, A and Miller, M. The role of the phospholipid sphingomyelin in heart disease. Current Opinion in Investigational D ...
... 81. Martin S, Williams N, and Chatterjee S. Lactosylceramide is required in apoptosis induced by Neutral sphingomyelinase. Glycoconjugate J. 23;147-157 2006. 82. Chatterjee,S Kolmakova, A and Miller, M. The role of the phospholipid sphingomyelin in heart disease. Current Opinion in Investigational D ...
nitrogen fixation and its improvement through genetic engineering
... Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitrogenase, are capable of utilizing atmospheric nitrogen. The thought of enginee ...
... Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitrogenase, are capable of utilizing atmospheric nitrogen. The thought of enginee ...
Complementary DNA Cloning, Messenger RNA
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
Identification and removal of colanic acid from plasmid DNA
... and have been shown to cause significant immune responses in animals.17 Investigators have reported inflammation and significant immune responses in animals after intramuscular injections of naked DNA,18 and the levels of these immune responses were identical to those produced after intramuscular in ...
... and have been shown to cause significant immune responses in animals.17 Investigators have reported inflammation and significant immune responses in animals after intramuscular injections of naked DNA,18 and the levels of these immune responses were identical to those produced after intramuscular in ...
Gene Section TSHR (thyroid stimulating hormone receptor) Atlas of Genetics and Cytogenetics
... 1.6 kb 3' UTR; the coding region spans positions 1572451 bp, with a signal peptide at 157-216 bp, mature peptide from 217 to 2448 bp, poly-A signal at 43714376. Encodes the canonical protein, 764 aa. NCBI Entrez Gene describes two alternatively spliced variants, TSHR isoform ...
... 1.6 kb 3' UTR; the coding region spans positions 1572451 bp, with a signal peptide at 157-216 bp, mature peptide from 217 to 2448 bp, poly-A signal at 43714376. Encodes the canonical protein, 764 aa. NCBI Entrez Gene describes two alternatively spliced variants, TSHR isoform ...
Gene Section CYP2A6 (cytochrome P450, family 2, subfamily A, polypeptide 6)
... CYP2A6 is an enzyme responsible for the metabolism of clinically used pharmaceuticals such as coumarin and valproic acid, several carcinogens such as 4(methylnitrosamine)-1-(3-pyridyl)-1-butanone and aflatoxin B1, nicotine and for the bioactivation process of tegafur to 5-FU. The formation of 5-FU f ...
... CYP2A6 is an enzyme responsible for the metabolism of clinically used pharmaceuticals such as coumarin and valproic acid, several carcinogens such as 4(methylnitrosamine)-1-(3-pyridyl)-1-butanone and aflatoxin B1, nicotine and for the bioactivation process of tegafur to 5-FU. The formation of 5-FU f ...
University of Groningen Lactococcus lactis systems biology Eckhardt
... of glycolysis for industrial use. The best-studied members of the LAB are Lactococcus, Lactobacillus, Leuconostoc, and Streptococcus. Other LAB family members are also of industrial interest, since some of these species are involved in fermented products or in food spoilage. The amount of research o ...
... of glycolysis for industrial use. The best-studied members of the LAB are Lactococcus, Lactobacillus, Leuconostoc, and Streptococcus. Other LAB family members are also of industrial interest, since some of these species are involved in fermented products or in food spoilage. The amount of research o ...
Assessment of the mosaic structure in the
... supplement S1). Moreover, alignment of the deduced amino acid sequences revealed frequent single amino acid mutations in the EPIYA/T segments (Supplement S1). H. pylori strain HJM6 and HJM12 possess an EPIYA/T-AABC genotype, whereas H. pylori strain HJM11 has an EPIYA/T-ABD genotype corresponding to ...
... supplement S1). Moreover, alignment of the deduced amino acid sequences revealed frequent single amino acid mutations in the EPIYA/T segments (Supplement S1). H. pylori strain HJM6 and HJM12 possess an EPIYA/T-AABC genotype, whereas H. pylori strain HJM11 has an EPIYA/T-ABD genotype corresponding to ...
FREE Solved Full Length Mock Test Paper
... phos phorylation and photophos phorylation? (A) Photophos phorylation occurs only in photosynthetic organisms and oxidative phos phorylation occurs only in non-photos ynthetic organisms . (B)In oxidative phosphorylation, NADH donates electrons ; in photophos phorylation ...
... phos phorylation and photophos phorylation? (A) Photophos phorylation occurs only in photosynthetic organisms and oxidative phos phorylation occurs only in non-photos ynthetic organisms . (B)In oxidative phosphorylation, NADH donates electrons ; in photophos phorylation ...
Altered pathogenicity, immunity, tissue tropism and 3` genome
... more other genes are responsible for the highly attenuated phenotype of the Beaudette IBV laboratory strain [13], and the roles of these gene products in the attenuation process has yet to be determined. In spite of the extensive use of vaccines, nephrotropic IBV outbreaks are frequent in China [33 ...
... more other genes are responsible for the highly attenuated phenotype of the Beaudette IBV laboratory strain [13], and the roles of these gene products in the attenuation process has yet to be determined. In spite of the extensive use of vaccines, nephrotropic IBV outbreaks are frequent in China [33 ...
Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus
... in the same medium. An aliquot was diluted 4-fold in 1 M Tris-HC1 pH 7.5 in the presence of 4% (v/v) ethyl methanesulfonate [17]. The mixture was shaken strongly at 26 "C and after 1 and 2 min 2-ml aliquots were removed, filtered through nitrocellulose filters and washed three times with 1 M Tris-HC ...
... in the same medium. An aliquot was diluted 4-fold in 1 M Tris-HC1 pH 7.5 in the presence of 4% (v/v) ethyl methanesulfonate [17]. The mixture was shaken strongly at 26 "C and after 1 and 2 min 2-ml aliquots were removed, filtered through nitrocellulose filters and washed three times with 1 M Tris-HC ...
Chapter 18
... LDLR-independent uptake of LDL (bad cholesterol) leads to formation of foam cell (LDLR play an important role of atherosclerosis??????? No no no no這不是LDLR的錯 ) Plasma LDL→ LDL in artery high → LDLR-mediated endocytosis ↑→rapidly foam cell develop ?????? However, LDL-mediated endocytosis is not a fact ...
... LDLR-independent uptake of LDL (bad cholesterol) leads to formation of foam cell (LDLR play an important role of atherosclerosis??????? No no no no這不是LDLR的錯 ) Plasma LDL→ LDL in artery high → LDLR-mediated endocytosis ↑→rapidly foam cell develop ?????? However, LDL-mediated endocytosis is not a fact ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.