Snorks Lab File
... protein molecules and that this is virtually the same mechanism for all life forms. ...
... protein molecules and that this is virtually the same mechanism for all life forms. ...
CH Zinc Fingers As DNA Binding Domains
... selective advantage over the original finger proteins to fiinction as transcription factors. This speculation is consistent with the fact that many of the separated-paired C2H2 and triple C2H2 zinc fingers conserve amino acid residues between tandem zinc fingers. After the duplication, point mutatio ...
... selective advantage over the original finger proteins to fiinction as transcription factors. This speculation is consistent with the fact that many of the separated-paired C2H2 and triple C2H2 zinc fingers conserve amino acid residues between tandem zinc fingers. After the duplication, point mutatio ...
Exogenous glutamine increases lipid accumulation in
... in vitro. A modified MS medium was supplied with 20% (m/v) polyethylene glycol 4,000 and 50 mM MES (2-(N-Morpholino)ethanesulfonic acid) were added to adjust osmotic potential, and the pH was adjusted to 5.8 with 1 N NaOH. The concentration of agar in MS medium was initially screened from 0.4% to 0. ...
... in vitro. A modified MS medium was supplied with 20% (m/v) polyethylene glycol 4,000 and 50 mM MES (2-(N-Morpholino)ethanesulfonic acid) were added to adjust osmotic potential, and the pH was adjusted to 5.8 with 1 N NaOH. The concentration of agar in MS medium was initially screened from 0.4% to 0. ...
Research in Microbiology
... 3.1. P. polymyxa Spo0A, Spo0B and Spo0F Signal transduction for sporulation is mediated by the phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is ...
... 3.1. P. polymyxa Spo0A, Spo0B and Spo0F Signal transduction for sporulation is mediated by the phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is ...
Journal of Bacteriology
... It has been shown that the sfp mutant CH3, which is devoid of nonribosomal synthesis of antimicrobial secondary metabolites, produced at least one substance that was effective against Bacillus subtilis CU1065 and particularly against its sigW mutant, HB0042 (8). SigW is an extracytoplasmic sigma fac ...
... It has been shown that the sfp mutant CH3, which is devoid of nonribosomal synthesis of antimicrobial secondary metabolites, produced at least one substance that was effective against Bacillus subtilis CU1065 and particularly against its sigW mutant, HB0042 (8). SigW is an extracytoplasmic sigma fac ...
PhD Thesis - Cox Group
... recognized for their pharmacological and biological activities. Natural products are also known as secondary metabolites as they are not crucial for basic life processes like growth and reproduction but assist the host organisms in their survival, facilitate interaction and communication, help in ad ...
... recognized for their pharmacological and biological activities. Natural products are also known as secondary metabolites as they are not crucial for basic life processes like growth and reproduction but assist the host organisms in their survival, facilitate interaction and communication, help in ad ...
SUC1 and SUC2: two sucrose transporters from Arabidopsis
... of conserved sequences has been identified in monosaccharide transporters. At the same positions a pair of ...
... of conserved sequences has been identified in monosaccharide transporters. At the same positions a pair of ...
Mini-Series: Modern Metabolic Concepts The Biochemistry of the
... lipids such as long chain fatty acids and cholesterol by lipogenic tissues (such as liver and adipose tissues and under special conditions in mammary glands during lactation and in the brain during the prenatal and early postnatal development). Additionally, amino acids from excess dietary protein a ...
... lipids such as long chain fatty acids and cholesterol by lipogenic tissues (such as liver and adipose tissues and under special conditions in mammary glands during lactation and in the brain during the prenatal and early postnatal development). Additionally, amino acids from excess dietary protein a ...
The DnaE polymerase from Deinococcus radiodurans features
... competence, D. radiodurans does also feature additional and peculiar biochemical properties responsible for genome integrity maintenance. In particular, when considering that ionizing radiations induce severe oxidative stress, it was realized that the radiation-resistance of D. radiodurans is mainly ...
... competence, D. radiodurans does also feature additional and peculiar biochemical properties responsible for genome integrity maintenance. In particular, when considering that ionizing radiations induce severe oxidative stress, it was realized that the radiation-resistance of D. radiodurans is mainly ...
Bacillus cereus
... Habitat; symbiosis; contributions to the environment. Pathology Considered non-pathogenic ...
... Habitat; symbiosis; contributions to the environment. Pathology Considered non-pathogenic ...
Characterization of sparfloxacin-resistant mutants of Staphylococcus aureus Original article
... eflux pumps remained stable and identical to the parenteral strain. There, the effect over the norfloxacin MIC was already 2– 2.6-fold (except for strain 4.32C which was four-fold), around six-fold over ciprofloxacin MIC, but very slight for sparfloxacin. Only the last strain (4.32H) showed an impor ...
... eflux pumps remained stable and identical to the parenteral strain. There, the effect over the norfloxacin MIC was already 2– 2.6-fold (except for strain 4.32C which was four-fold), around six-fold over ciprofloxacin MIC, but very slight for sparfloxacin. Only the last strain (4.32H) showed an impor ...
the structure and function of cartilage proteoglycans
... varies between species (Barry et al., 1994). The neighbouring CS-attachment domain is divided into two subdomains – the CS1 and CS2 domains. The CS1 domain lies adjacent to the KS-rich domain and is also composed largely of repeat motifs whose number varies between species. In addition, the human CS ...
... varies between species (Barry et al., 1994). The neighbouring CS-attachment domain is divided into two subdomains – the CS1 and CS2 domains. The CS1 domain lies adjacent to the KS-rich domain and is also composed largely of repeat motifs whose number varies between species. In addition, the human CS ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... descriptions of amino acids. Sail sites were created in pRGA39and pCGA239by in vitro mutagenesis (see Materials and Methods) resulting in pRGA39sand pCGA239s, respectively. pCGA439was constructed by recombination ofpRGA39s and pCGA239sat Hindlll and sail sites. The altered amino acid (D) was restore ...
... descriptions of amino acids. Sail sites were created in pRGA39and pCGA239by in vitro mutagenesis (see Materials and Methods) resulting in pRGA39sand pCGA239s, respectively. pCGA439was constructed by recombination ofpRGA39s and pCGA239sat Hindlll and sail sites. The altered amino acid (D) was restore ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
... were imported by this pharmaceutical company from Africa in connection with experiments to establish kidney cell cultures that were needed to develop a vaccine against poliomyelitis. In a tour de force, and without precise knowledge of the danger that lurked in handling infected patients, tissue, an ...
... were imported by this pharmaceutical company from Africa in connection with experiments to establish kidney cell cultures that were needed to develop a vaccine against poliomyelitis. In a tour de force, and without precise knowledge of the danger that lurked in handling infected patients, tissue, an ...
Highly Conserved Region 141–168 of the NS1 Protein Is a New
... Thus, in this study, three different antigens, namely a mixture of DENV 1–4 particles with/without inactivation and rNS1 protein, were used for immunization, and cell lysates derived from DENV 1–4-infected BHK cells were used for boosting. In contrast, in other studies, NS1 purified by affinity colu ...
... Thus, in this study, three different antigens, namely a mixture of DENV 1–4 particles with/without inactivation and rNS1 protein, were used for immunization, and cell lysates derived from DENV 1–4-infected BHK cells were used for boosting. In contrast, in other studies, NS1 purified by affinity colu ...
The aconitase of Escherichia cok purification of the
... University of Shefield, PO Box 594,Firth Court, Western Bank, Shefield SIO 2 U H , UK (Received 24 April 1991; revised I0 July 1991;accepted 24 July 1991) The aconitase of Escherichia cofi was purified to homogeneity, albeit in low yield (0.6%). It was shown to be a monomeric protein of Mr95000 or 9 ...
... University of Shefield, PO Box 594,Firth Court, Western Bank, Shefield SIO 2 U H , UK (Received 24 April 1991; revised I0 July 1991;accepted 24 July 1991) The aconitase of Escherichia cofi was purified to homogeneity, albeit in low yield (0.6%). It was shown to be a monomeric protein of Mr95000 or 9 ...
Protein translation in Plasmodium parasites
... Plasmodium with these five essential amino acids is sufficient for short-term parasite development [17], and recent evidence has shown that Ile alone is sufficient to support parasite growth [16]. However, for some Plasmodium strains Met supplementation is also required for optimal growth [16]. The ...
... Plasmodium with these five essential amino acids is sufficient for short-term parasite development [17], and recent evidence has shown that Ile alone is sufficient to support parasite growth [16]. However, for some Plasmodium strains Met supplementation is also required for optimal growth [16]. The ...
Exploring Mouse Protein Function via Multiple Approaches
... explore the functional relationships between interacting proteins. There are many computational models for predicting protein-protein interactions [22–24]. The commonly accepted hypothesis (called guilt-by-association (GBA) [25]) is that proteins are more likely to share identical or similar functio ...
... explore the functional relationships between interacting proteins. There are many computational models for predicting protein-protein interactions [22–24]. The commonly accepted hypothesis (called guilt-by-association (GBA) [25]) is that proteins are more likely to share identical or similar functio ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
... Several enzymes are involved in Capsaicin biosynthesis. Capsaicin synthase (CS) is the last enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransfera ...
... Several enzymes are involved in Capsaicin biosynthesis. Capsaicin synthase (CS) is the last enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransfera ...
as a PDF
... presumed TRAP binding site (35). This site contains as many as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it could be regulated by uncharged tRNATrp (35). yczA is preceded b ...
... presumed TRAP binding site (35). This site contains as many as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it could be regulated by uncharged tRNATrp (35). yczA is preceded b ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
Cloning and Characterization of Human Urocortin
... motif is highly conserved in the CRF peptide family, seen in all CRFs, Uros, and Svg. This change is further intriguing becausea Pro-Ser, rather than the Pro-Pro consensus, occurs in some of the insect diuretic hormones (24,25), which have also been considered to be part of the CRF superfamily. Amon ...
... motif is highly conserved in the CRF peptide family, seen in all CRFs, Uros, and Svg. This change is further intriguing becausea Pro-Ser, rather than the Pro-Pro consensus, occurs in some of the insect diuretic hormones (24,25), which have also been considered to be part of the CRF superfamily. Amon ...
New lysosomal acid lipase gene mutants explain the phenotype of
... with CESD (4, 7–9). Skipping of the same exon has also been observed in a Wolman disease patient. However, in this case, the G to A substitution was in the position 11 of the 59 splice site of the exon (10). Other splicing defects, point mutations resulting in single amino acid substitutions, and sm ...
... with CESD (4, 7–9). Skipping of the same exon has also been observed in a Wolman disease patient. However, in this case, the G to A substitution was in the position 11 of the 59 splice site of the exon (10). Other splicing defects, point mutations resulting in single amino acid substitutions, and sm ...
Placental Transporters Relevant to Drug Distribution across the
... transporter (Balkovetz et al., 1988) as well as the dicarboxylate transporter (Ganapathy et al., 1988b). Functional studies with intact placenta and the choriocarcinoma cell line BeWo have suggested that the placental basal membrane may also possess the monocarboxylate transport mechanism (Carstense ...
... transporter (Balkovetz et al., 1988) as well as the dicarboxylate transporter (Ganapathy et al., 1988b). Functional studies with intact placenta and the choriocarcinoma cell line BeWo have suggested that the placental basal membrane may also possess the monocarboxylate transport mechanism (Carstense ...
Chlamydia Exploit the Mammalian Tryptophan-Depletion
... forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. ...
... forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.