Steroid Chemistry and Steroid Hormone Action - Rose
... ligands (dissociation constant usually less than 1 nanomolar). They function as ligand activated transcription factors, specifically activating a small number of genes (less than 50, and possibly less than 10 genes per cell). The Steroid Hormone Receptor Superfamily All of the characterized steroid ...
... ligands (dissociation constant usually less than 1 nanomolar). They function as ligand activated transcription factors, specifically activating a small number of genes (less than 50, and possibly less than 10 genes per cell). The Steroid Hormone Receptor Superfamily All of the characterized steroid ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... in unfertilized amphibian eggs by Masui and Markert (1971) and Smith and Ecker (1971) as an activity capable of inducing amphibian oocytes to enter meiosis. It has subsequently been identified in a wide variety of meiotic and mitotic cells from yeast to man (Kishimoto and Kanatani, 1976; Wasserman a ...
... in unfertilized amphibian eggs by Masui and Markert (1971) and Smith and Ecker (1971) as an activity capable of inducing amphibian oocytes to enter meiosis. It has subsequently been identified in a wide variety of meiotic and mitotic cells from yeast to man (Kishimoto and Kanatani, 1976; Wasserman a ...
Mutant selection and phenotypic and genetic characterization of
... including four with identical genetic changes. Specifically genes Cthe0390 (putative glucokinase), Cthe1866 (argD, acetylornithine aminotransferase), Cthe2699 (putative transcriptional regulator), and Cthe2870 (protein of unknown function) had identical changes in both strains. Cthe0423 (adhE, bi-fu ...
... including four with identical genetic changes. Specifically genes Cthe0390 (putative glucokinase), Cthe1866 (argD, acetylornithine aminotransferase), Cthe2699 (putative transcriptional regulator), and Cthe2870 (protein of unknown function) had identical changes in both strains. Cthe0423 (adhE, bi-fu ...
Inflammation and ER Stress Regulate Branched
... liver, and expression was found to not be affected and, in the case of liver, was increased (Figure 1D). These results demonstrate the pathway-specific down regulation of the BCAA metabolism enzymes in visceral adipose tissue. Immune cell-derived inflammatory factors have been shown to down-regulate ...
... liver, and expression was found to not be affected and, in the case of liver, was increased (Figure 1D). These results demonstrate the pathway-specific down regulation of the BCAA metabolism enzymes in visceral adipose tissue. Immune cell-derived inflammatory factors have been shown to down-regulate ...
Structure, function and regulation of pyruvate carboxylase
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
... Role of PC in insulin signalling in pancreatic islets Glucose is a potent stimulator of insulin secretion from βpancreatic cells when extracellular levels are greater than 3 mM. Secretion of insulin in response to a high concentration of glucose results in the rapid uptake of glucose by pancreatic β ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
... agarose gel (EuroClone, UK) by fluorochromatizaton with ethidium bromide. The detected amplicons were excised from the gel and purified on a JETquick spin column kit (Genomed, USA), as suggested by the manufacturer. For 39-RACE the first strand cDNA synthesized by the poly(T)-anchor primer was purif ...
... agarose gel (EuroClone, UK) by fluorochromatizaton with ethidium bromide. The detected amplicons were excised from the gel and purified on a JETquick spin column kit (Genomed, USA), as suggested by the manufacturer. For 39-RACE the first strand cDNA synthesized by the poly(T)-anchor primer was purif ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... 2. Guttacher: Prof. Dr. Nediljko Budisa Mündliche Prüfung am: 07/06/2010 ...
... 2. Guttacher: Prof. Dr. Nediljko Budisa Mündliche Prüfung am: 07/06/2010 ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... New virus (e.g. SARS) and goal to develop treatment Scientists isolate genetic material of virus Screen genome for relationships with previously studied viruses [10] From virus’ DNA they compute the proteins it produces [1] Compute proteins’ three-dimensional structure and thereby obtain clues about ...
... New virus (e.g. SARS) and goal to develop treatment Scientists isolate genetic material of virus Screen genome for relationships with previously studied viruses [10] From virus’ DNA they compute the proteins it produces [1] Compute proteins’ three-dimensional structure and thereby obtain clues about ...
The different roles of tryptophan transfer RNA in regulating trp
... pause or anti-antiterminator; 2:3, an antiterminator; and 3:4, a terminator [2]. These numbers refer to sequential segments of the trp operon leader transcript (Figure 2a). Structure 3:4 is an intrinsic terminator. Whenever this structure forms it directs the transcribing RNA polymerase to terminate ...
... pause or anti-antiterminator; 2:3, an antiterminator; and 3:4, a terminator [2]. These numbers refer to sequential segments of the trp operon leader transcript (Figure 2a). Structure 3:4 is an intrinsic terminator. Whenever this structure forms it directs the transcribing RNA polymerase to terminate ...
ATPase isoforms in human kidney - AJP
... rabbit collecting ducts. Transport of these ions appears to be accomplished by one or both of two isoforms of the H⫹-K⫹ATPase, HK␣1 and HK␣2, because both isoforms are found in the collecting ducts and transport of hydrogen and potassium is attenuated by exposure to inhibitors of these transport pro ...
... rabbit collecting ducts. Transport of these ions appears to be accomplished by one or both of two isoforms of the H⫹-K⫹ATPase, HK␣1 and HK␣2, because both isoforms are found in the collecting ducts and transport of hydrogen and potassium is attenuated by exposure to inhibitors of these transport pro ...
PpASCL, a moss ortholog of antherspecific chalcone synthaselike
... a-pyrone reductase (TKPR) to polyhydroxyalkyl a-pyrones that serve as building blocks of sporopollenin. ...
... a-pyrone reductase (TKPR) to polyhydroxyalkyl a-pyrones that serve as building blocks of sporopollenin. ...
Phosphoketolase pathway dominates in
... parameters employed were as follows: a 60-degree pulse length (6.6 s), a sweep width of 12 kHz (16,000 complex data points), 30,000 scans, a 2.0-s recycle time with waltz16 proton decoupling applied in a bilevel power scheme to minimize the effects of heating. The data were zero filled to 32,000 an ...
... parameters employed were as follows: a 60-degree pulse length (6.6 s), a sweep width of 12 kHz (16,000 complex data points), 30,000 scans, a 2.0-s recycle time with waltz16 proton decoupling applied in a bilevel power scheme to minimize the effects of heating. The data were zero filled to 32,000 an ...
Insulin-like growth factor binding protein-2 (IGFBP
... pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplification, 1 lL cDNA template, 2 lL of 109 Ex Taq buffer, 1.6 lL of dNTP (2.5 mM of each), 1 lL of the forwa ...
... pairs of primers (IGFBP-2a-f, IGFBP-2a-r and IGFBP2b-f, IGFBP-2b-r) were designed in non-conservative region for cloning and verifying the partial fragment of P. olivaceus IGFBP-2. In PCR amplification, 1 lL cDNA template, 2 lL of 109 Ex Taq buffer, 1.6 lL of dNTP (2.5 mM of each), 1 lL of the forwa ...
phylogenetic analysis of the rompb genes of rickettsia felis and
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
... and outer membrane location and the strong immune response observed after infection, suggesting an important yet undefined function under evolutionary selective pressure.1,2 Although the rOmpB encoding gene (rompB) is present in both spotted fever group (SFG) and typhus group (TG) rickettsiae, it ha ...
Experimental Analysis of the Rice Mitochondrial
... Mitochondria in rice (Oryza sativa) are vital in expanding our understanding of the cellular response to reoxygenation of tissues after anaerobiosis, the crossroads of carbon and nitrogen metabolism, and the role of respiratory energy generation in cytoplasmic male sterility. We have combined densit ...
... Mitochondria in rice (Oryza sativa) are vital in expanding our understanding of the cellular response to reoxygenation of tissues after anaerobiosis, the crossroads of carbon and nitrogen metabolism, and the role of respiratory energy generation in cytoplasmic male sterility. We have combined densit ...
Exploration of mutations in erythroid 5
... Richfield, two of the most hardworking, loving, and inspiring people I will have ever known. I also dedicate this work to my amazing parents, Donald Douglas Fratz and Naomi Richfield-Fratz, who inspire me to follow my dreams and support me through all of my endeavors. Lastly, I dedicate this dissert ...
... Richfield, two of the most hardworking, loving, and inspiring people I will have ever known. I also dedicate this work to my amazing parents, Donald Douglas Fratz and Naomi Richfield-Fratz, who inspire me to follow my dreams and support me through all of my endeavors. Lastly, I dedicate this dissert ...
Transcriptional Regulation of Metabolism
... mutation in the enzyme glucokinase, MODY is due to mutations in genes encoding transcription factors involved in insulin gene expression (see Fig. 2). Alteration of HNF1␣, which causes MODY3, is the most frequent transcription factor defect leading to MODY, whereas MODY1 is rare and due to mutations ...
... mutation in the enzyme glucokinase, MODY is due to mutations in genes encoding transcription factors involved in insulin gene expression (see Fig. 2). Alteration of HNF1␣, which causes MODY3, is the most frequent transcription factor defect leading to MODY, whereas MODY1 is rare and due to mutations ...
Neural Marker Antibodies Epitope Tag Antibodies
... of alternative names, including neuromodulin and F1. In cultured neurons, GAP-43 antibodies selectively stains the growth cones of axons. As the substrate for various phosphorylation events, it is believed that GAP-43 plays a critical role in axonal growth, although its exact function is still uncle ...
... of alternative names, including neuromodulin and F1. In cultured neurons, GAP-43 antibodies selectively stains the growth cones of axons. As the substrate for various phosphorylation events, it is believed that GAP-43 plays a critical role in axonal growth, although its exact function is still uncle ...
Biochemical and Molecular Characterization of the Chicken
... proteins that are believed to play a structural role in cell substratum adhesion, such as vinculin (Geiger, 1979; Otto, 1990), talin (Burridge and Connell, 1983; Beckerle and Yeh, 1990) and c~-actinin (Lazarides and Burridge, 1975; Blanchard et al., 1989). In addition, a number of low abundance prot ...
... proteins that are believed to play a structural role in cell substratum adhesion, such as vinculin (Geiger, 1979; Otto, 1990), talin (Burridge and Connell, 1983; Beckerle and Yeh, 1990) and c~-actinin (Lazarides and Burridge, 1975; Blanchard et al., 1989). In addition, a number of low abundance prot ...
Bacterial hemoglobins and flavohemoglobins: versatile proteins and
... primary use as a substrate and its secondary e¡ects on metabolism. Its use as a substrate allows cellular metabolism to work at optimal levels of substrate utilization and energy yield. Oxygen is required for the regulation of a variety of cellular functions, which are expressed in response to oxyge ...
... primary use as a substrate and its secondary e¡ects on metabolism. Its use as a substrate allows cellular metabolism to work at optimal levels of substrate utilization and energy yield. Oxygen is required for the regulation of a variety of cellular functions, which are expressed in response to oxyge ...
A Metabolic Node in Action: Chorismate
... and is retro-inhibited by Tyr (45% for the CM and 95% for the dehydrogenase activity).44,45 It is now evident that the mutase and the dehydrogenase active sites are distinct, but display some similar chemical properties.46-50 Kinetic studies have shown that Tyr binds twice at distinct allosteric sit ...
... and is retro-inhibited by Tyr (45% for the CM and 95% for the dehydrogenase activity).44,45 It is now evident that the mutase and the dehydrogenase active sites are distinct, but display some similar chemical properties.46-50 Kinetic studies have shown that Tyr binds twice at distinct allosteric sit ...
C-terminal Truncation of p21H Preserves Crucial Kinetic and
... either at position 12/13 or 59/61 (for reviews see Refs. 1-3). The p21 protein products of the Ha-, K-, and N-ras genes have identical amino acid sequences for the first 80 amino acids and aremore than 85%identical up to amino acids 1641 165. The C-terminal25amino acids are very divergent, except fo ...
... either at position 12/13 or 59/61 (for reviews see Refs. 1-3). The p21 protein products of the Ha-, K-, and N-ras genes have identical amino acid sequences for the first 80 amino acids and aremore than 85%identical up to amino acids 1641 165. The C-terminal25amino acids are very divergent, except fo ...
Substitutions and Deletions in the Cytoplasmic
... The cells were then stained with Wright-Giemsa and the phagocytosis by FcyRIIA is shown in Fig 2. Replacement number of COS-l cells with one or more internalized EA was deterof the first tyrosine (which is not within a typical Y-x-x-L mined in a blinded fashion. Phagocytosis was expressed as phagomo ...
... The cells were then stained with Wright-Giemsa and the phagocytosis by FcyRIIA is shown in Fig 2. Replacement number of COS-l cells with one or more internalized EA was deterof the first tyrosine (which is not within a typical Y-x-x-L mined in a blinded fashion. Phagocytosis was expressed as phagomo ...
Relationship Between the Occurrence of Cysteine in Proteins and
... expansion of the genetic code is primarily based on the anomalous codon allocations of various species (Osawa et al. 1992; Bauman and Oro 1993). In addition, Doring and Marliere (1998) showed that mutant tRNAs that incorporate cysteine at positions corresponding to the isoleucine or methionine codon ...
... expansion of the genetic code is primarily based on the anomalous codon allocations of various species (Osawa et al. 1992; Bauman and Oro 1993). In addition, Doring and Marliere (1998) showed that mutant tRNAs that incorporate cysteine at positions corresponding to the isoleucine or methionine codon ...
Genome segment 5 of Antheraea mylitta cytoplasmic polyhedrosis
... Background: Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV), a cypovirus of Reoviridae family, infects non mulberry Indian silk worm, Antheraea mylitta, and contains eleven segmented double stranded RNA in its genome (S1-S11). Some of its genome segments (S1-S3, and S6-S11) have been previo ...
... Background: Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV), a cypovirus of Reoviridae family, infects non mulberry Indian silk worm, Antheraea mylitta, and contains eleven segmented double stranded RNA in its genome (S1-S11). Some of its genome segments (S1-S3, and S6-S11) have been previo ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.