Preparation of Lentiviral expression construct DNA
... quantification of p53 in a 96-well format . Double-stranded oligonucleotides containing a p53 consensus binding site are immobilized in a 96-well plate. The p53 proteins present in nuclear extracts are captured by the immobilized oligonucleotides specifically and then detected by a p53 antibody and ...
... quantification of p53 in a 96-well format . Double-stranded oligonucleotides containing a p53 consensus binding site are immobilized in a 96-well plate. The p53 proteins present in nuclear extracts are captured by the immobilized oligonucleotides specifically and then detected by a p53 antibody and ...
subunits of succinyl CoA ligase of tomato
... Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNA ...
... Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNA ...
GST II: ---Title--- - Digital Biology Laboratory
... for probability of one amino acid being substituted for another: frequency of substitution - genetic code tolerance for changes - natural selection penalize residues pairs with a low probability of mutation in evolution and rewards pairs with a high probability empirically derived from observed ...
... for probability of one amino acid being substituted for another: frequency of substitution - genetic code tolerance for changes - natural selection penalize residues pairs with a low probability of mutation in evolution and rewards pairs with a high probability empirically derived from observed ...
... 13. (10 pts) Please do one of the following five choices. Please indicate your choice. Choice A: What type of chemical change generates energy in degradative metabolic pathways? Provide one example of this change, including cofactors/cosubstrates, and give the generic name of the enzyme that catalyz ...
Coordinated concentration changes of transcript and metabolites in Saccharomyces cerevisiae.
... unlike transcriptional or protein-interaction networks, consist of molecular species which chemically interconvert. As a result, metabolites that are only distantly related in terms of the underlying pathways can show high levels of correlation [18]. This is especially true in the case of global per ...
... unlike transcriptional or protein-interaction networks, consist of molecular species which chemically interconvert. As a result, metabolites that are only distantly related in terms of the underlying pathways can show high levels of correlation [18]. This is especially true in the case of global per ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... peroxisome proliferator activated receptor (PPAR) responsive genes (Uren-Webster et al., 2010). In our previous study, DEHP is found to mediate immune responses in clams by triggering the production of reactive oxygen species and nitric oxide (Lu et al., 2013). During the experiment, a strong decrea ...
... peroxisome proliferator activated receptor (PPAR) responsive genes (Uren-Webster et al., 2010). In our previous study, DEHP is found to mediate immune responses in clams by triggering the production of reactive oxygen species and nitric oxide (Lu et al., 2013). During the experiment, a strong decrea ...
Red blood cells: proteomics, physiology and metabolism
... 3.1 Physiology and proteomics The RBC membrane is critical to maintenance of the characteristic biconcave shape of the RBC; it ensures appropriate pH and cation concentration differentials between the RBC and the plasma (low potassium, high sodium and calcium), an adequate area to volume ratio, flui ...
... 3.1 Physiology and proteomics The RBC membrane is critical to maintenance of the characteristic biconcave shape of the RBC; it ensures appropriate pH and cation concentration differentials between the RBC and the plasma (low potassium, high sodium and calcium), an adequate area to volume ratio, flui ...
A method for measuring the non-random bias of a codon usage table
... amino acid composition of typical proteins (5): some amino acids are used far more frequently than others. ...
... amino acid composition of typical proteins (5): some amino acids are used far more frequently than others. ...
results and discussion discussion
... LytT, response regulator of the LytR/AlgR (COG3279), response regulator containing CheY-like receiver and SARP (COG3947), CheB, chemotaxis response regulator containing a CheY-like receiver and a methylesterase ( COG2201), CitB, response regulator containing a CheY-like receiver and an HTH DNA-bindi ...
... LytT, response regulator of the LytR/AlgR (COG3279), response regulator containing CheY-like receiver and SARP (COG3947), CheB, chemotaxis response regulator containing a CheY-like receiver and a methylesterase ( COG2201), CitB, response regulator containing a CheY-like receiver and an HTH DNA-bindi ...
Phylogenetic and evolutionary analyses of St. Louis encephalitis
... sequences to conduct phylogenetic analyses, in order to investigate the forces shaping the evolution of St. Louis encephalitis virus. Contrary to previous reports, we found little evidence for recombination in these isolates. Most of the amino acid sites in the SLEV polyprotein appeared to be under ...
... sequences to conduct phylogenetic analyses, in order to investigate the forces shaping the evolution of St. Louis encephalitis virus. Contrary to previous reports, we found little evidence for recombination in these isolates. Most of the amino acid sites in the SLEV polyprotein appeared to be under ...
nitrogen assimilation and global regulation in escherichia coli
... Annu. Rev. Microbiol. 2003.57:155-176. Downloaded from arjournals.annualreviews.org by WIB6332 - University of Saarland on 01/04/10. For personal use only. ...
... Annu. Rev. Microbiol. 2003.57:155-176. Downloaded from arjournals.annualreviews.org by WIB6332 - University of Saarland on 01/04/10. For personal use only. ...
Analysis of a ribose transport operon from Bacillus
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
Mitochondrial DNA and aging
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
Isotope-labeled Peptides
... It requires the synthesis of a stable isotopelabeled peptide chemically identical to one the peptides generated by the tryptic digestion of the protein to be measured. A known quantity of this labeled peptide is used as an internal standard against which the chosen tryptic peptide can be quantified. ...
... It requires the synthesis of a stable isotopelabeled peptide chemically identical to one the peptides generated by the tryptic digestion of the protein to be measured. A known quantity of this labeled peptide is used as an internal standard against which the chosen tryptic peptide can be quantified. ...
Datasheet - Sigma
... homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants ...
... homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants ...
An isoform of the cGMP-gated retinal photoreceptor channel gene
... coupling of appropriate receptors/second messenger systems to gate the channel in the heart; these are unlikely to be related to those types co-expressed in the photoreceptor. Thirdly, the gene sequence for the human cGMP-gated channel has been determined [5] and contains many potential splice sites ...
... coupling of appropriate receptors/second messenger systems to gate the channel in the heart; these are unlikely to be related to those types co-expressed in the photoreceptor. Thirdly, the gene sequence for the human cGMP-gated channel has been determined [5] and contains many potential splice sites ...
STRIVE Report Series No.65
... other stakeholders on a range of questions in relation to environmental protection. These reports are intended as contributions to the necessary debate on the protection of the environment. ...
... other stakeholders on a range of questions in relation to environmental protection. These reports are intended as contributions to the necessary debate on the protection of the environment. ...
Document
... Accepted Mutation; they were proposed in 1978, by M. Dayhoff et al., on the basis of a study on molecular phylogeny involving 71 protein families PAM matrices were developed by examining mutations within superfamilies of closely related proteins, also noting how observed substitutions did not happen ...
... Accepted Mutation; they were proposed in 1978, by M. Dayhoff et al., on the basis of a study on molecular phylogeny involving 71 protein families PAM matrices were developed by examining mutations within superfamilies of closely related proteins, also noting how observed substitutions did not happen ...
0 - Microbiology
... (capsulated) progeny, but could also cause non-capsulated organisms of a different strain to yield capsulated progeny that exhibited the serological characteristics of the heat-killed donor organisms. This phenomenon, to which Griffith gave the name ‘transformation’, was ultimately shown to be due t ...
... (capsulated) progeny, but could also cause non-capsulated organisms of a different strain to yield capsulated progeny that exhibited the serological characteristics of the heat-killed donor organisms. This phenomenon, to which Griffith gave the name ‘transformation’, was ultimately shown to be due t ...
Variations in amino acid composition in bacterial single stranded
... Two domains with three distinctive elements can be found in the SSBs: N-terminal domain which forms DNA-binding domain (OB-fold), and C-terminal domain which is a largely unstructured region often rich in glycine and proline residues with a conserved acidic Cterminal motif. While studying structure/ ...
... Two domains with three distinctive elements can be found in the SSBs: N-terminal domain which forms DNA-binding domain (OB-fold), and C-terminal domain which is a largely unstructured region often rich in glycine and proline residues with a conserved acidic Cterminal motif. While studying structure/ ...
Metabolic rate depression in animals
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
Brucella Quorum Sensing: much more than
... in which they identified fifteen candidate genes whose products are suspected to interact with the AHL metabolic precursors SAM or acyl-ACP. Further, the candidate gene’s expression was altered by addition of C12-HSL or by mutation of another QS regulator (VjbR see below). However, expression of the ...
... in which they identified fifteen candidate genes whose products are suspected to interact with the AHL metabolic precursors SAM or acyl-ACP. Further, the candidate gene’s expression was altered by addition of C12-HSL or by mutation of another QS regulator (VjbR see below). However, expression of the ...
Carotene genes from cassava-pchavarriaga.pdf
... Carotenoids are present in all photosynthetic organisms, acting in plants as accessory pigments in light harvesting complexes in the thylakoids of chloroplasts. They confer protection against photooxidative stress and are also precursors for abscisic acid. In recent years, genes that code for enzime ...
... Carotenoids are present in all photosynthetic organisms, acting in plants as accessory pigments in light harvesting complexes in the thylakoids of chloroplasts. They confer protection against photooxidative stress and are also precursors for abscisic acid. In recent years, genes that code for enzime ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.