Identifying producers of antibacterial compounds by
... Microbially derived natural products are major sources of antibiotics and other medicines, but discovering new antibiotic scaffolds and increasing the chemical diversity of existing ones are formidable challenges. We have designed a screen to exploit the self-protection mechanism of antibiotic produ ...
... Microbially derived natural products are major sources of antibiotics and other medicines, but discovering new antibiotic scaffolds and increasing the chemical diversity of existing ones are formidable challenges. We have designed a screen to exploit the self-protection mechanism of antibiotic produ ...
Structural and functional features of Drosophila chorion proteins s36
... encoded by genes tightly clustered on the third chromosome, and s36, s38 by genes clustered on the X chromosome 8-~°. These genes are products of specific DNA replication (gene amplification). Proteins s15, s16, s18 and s19 show obvious homologies in their primary structure. This is also true for th ...
... encoded by genes tightly clustered on the third chromosome, and s36, s38 by genes clustered on the X chromosome 8-~°. These genes are products of specific DNA replication (gene amplification). Proteins s15, s16, s18 and s19 show obvious homologies in their primary structure. This is also true for th ...
Pathways to abscisic acid-regulated gene expression
... data that implicate ABA in stress responses and developmental programs, the adaptive responses of plants to ABA and stresses, and the pathways that trigger them, are largely unknown. The nature of the cellular responses to ABA is a function of the cell type, and there might be separate ABA signallin ...
... data that implicate ABA in stress responses and developmental programs, the adaptive responses of plants to ABA and stresses, and the pathways that trigger them, are largely unknown. The nature of the cellular responses to ABA is a function of the cell type, and there might be separate ABA signallin ...
Review Non-ribosomal peptide synthetases: Identifying the cryptic
... motifs with that of GrsA (Gramicidin S synthetase, phenylalanine activating A-domain). A specific Stachelhaus code exists for a particular substrate and if an identical code is present in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. ...
... motifs with that of GrsA (Gramicidin S synthetase, phenylalanine activating A-domain). A specific Stachelhaus code exists for a particular substrate and if an identical code is present in an uncharacterized A-domain, then the probability of their substrates being the same is high (Stachelhaus et al. ...
Activation of Peroxisome Proliferator-Activated Receptors
... common and persistent environmental enhanced by their metabolism catalyzed by CYP4A enzymes (10-14). Moreover, pollutant, and has been found in over one- liver cytochrome P450 (CYP) enzymes, chronic administration of DHEA can lead third of hazardous waste sites and in 10% of which produce multiple r ...
... common and persistent environmental enhanced by their metabolism catalyzed by CYP4A enzymes (10-14). Moreover, pollutant, and has been found in over one- liver cytochrome P450 (CYP) enzymes, chronic administration of DHEA can lead third of hazardous waste sites and in 10% of which produce multiple r ...
The KIebsieIIa pneumoniae cytochrome bd
... Cytochrome bd‘ has been implicated in having an important role in microaerobic nitrogen fixation in the enteric bacterium Klebsiella pneumoniae, where it is expressed under all conditions that permit diazotrophy. In this paper the sequence of the genes encoding this terminal oxidase (cydAB) of Klebs ...
... Cytochrome bd‘ has been implicated in having an important role in microaerobic nitrogen fixation in the enteric bacterium Klebsiella pneumoniae, where it is expressed under all conditions that permit diazotrophy. In this paper the sequence of the genes encoding this terminal oxidase (cydAB) of Klebs ...
Transcription Factor EB Controls Metabolic Flexibility during
... fibers underwent a significant depolarization after oligomycin treatment (Figure 2G), suggesting that these fibers were at least in part relying on reverse activity of ATP synthase to preserve their membrane potential as a consequence of proton membrane leak. In addition, oxidative stress, revealed ...
... fibers underwent a significant depolarization after oligomycin treatment (Figure 2G), suggesting that these fibers were at least in part relying on reverse activity of ATP synthase to preserve their membrane potential as a consequence of proton membrane leak. In addition, oxidative stress, revealed ...
Comparative analysis of proteins with a mucus
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
... The putative MUB domain of the protein lp_1229 of Lb. plantarum WCFS1 consists of approximately 100 aa, while the MUB domain of protein Mub of Lb. reuteri is almost 200 residues in length (Kleerebezem et al., 2003; Pretzer et al., 2005; Roos & Jonsson, 2002). This difference in size implies a discre ...
Structure of a Plasmodium yoelii gene
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
Metabolic adaptation of Mycobacterium avium subsp
... causes Johne’s disease, a chronic and incurable granulomatous enteritis of ruminants, and has been suggested to be a putative aetiological agent of Crohn’s disease in humans. Using a comprehensive LC-MS-MS and 2D difference gel electrophoresis (DIGE) approach, we compared the protein profiles of cli ...
... causes Johne’s disease, a chronic and incurable granulomatous enteritis of ruminants, and has been suggested to be a putative aetiological agent of Crohn’s disease in humans. Using a comprehensive LC-MS-MS and 2D difference gel electrophoresis (DIGE) approach, we compared the protein profiles of cli ...
Expression of the LXR Protein in Human Atherosclerotic Lesions
... Systemic administration of an LXR agonist reduced atherosclerosis in LDLR⫺/⫺ and apoE⫺/⫺ mice.13 Further, bone marrow transplantation from LXR␣/⫺/⫺ mice increased lesion formation in these same models.14 These results suggested that LXR␣ expressed in macrophages in atherosclerotic lesions may play ...
... Systemic administration of an LXR agonist reduced atherosclerosis in LDLR⫺/⫺ and apoE⫺/⫺ mice.13 Further, bone marrow transplantation from LXR␣/⫺/⫺ mice increased lesion formation in these same models.14 These results suggested that LXR␣ expressed in macrophages in atherosclerotic lesions may play ...
chapter 1: exploring life
... controlled experiment data deductive reasoning deoxyribonucleic acid (DNA) discovery science ecosystem emergent properties Eukarya eukaryotic cell Fungi gene genome hypothesis inductive reasoning inquiry model molecule natural selection negative feedback organ organ system organelle organism plantae ...
... controlled experiment data deductive reasoning deoxyribonucleic acid (DNA) discovery science ecosystem emergent properties Eukarya eukaryotic cell Fungi gene genome hypothesis inductive reasoning inquiry model molecule natural selection negative feedback organ organ system organelle organism plantae ...
BIOS QTL browser - BBMRI-NL
... Introduction The BIOS consortium aims at integrating these layers of data, in order to uncover molecular pathways involved in health and disease. ...
... Introduction The BIOS consortium aims at integrating these layers of data, in order to uncover molecular pathways involved in health and disease. ...
Proteomic Analysis of Neisseria gonorrhoeae Biofilms
... Neisseria gonorrhoeae, the causative agent of gonorrhea, can form biofilms in vitro and in vivo. In biofilms, the organism is more resistant to antibiotic treatment and can serve as a reservoir for chronic infection. We have used stable isotope labeling by amino acids in cell culture (SILAC) to comp ...
... Neisseria gonorrhoeae, the causative agent of gonorrhea, can form biofilms in vitro and in vivo. In biofilms, the organism is more resistant to antibiotic treatment and can serve as a reservoir for chronic infection. We have used stable isotope labeling by amino acids in cell culture (SILAC) to comp ...
Homology Modelling and Structural Comparisons of Capsid
... elude the host immune system, and can be related to the publication from Kundu et al. [21], showing that viruses infecting the same population may have diverse biological fitness due to genetic mutations that translate into slight amino acid variation. Specifically, Cap must successfully evade the h ...
... elude the host immune system, and can be related to the publication from Kundu et al. [21], showing that viruses infecting the same population may have diverse biological fitness due to genetic mutations that translate into slight amino acid variation. Specifically, Cap must successfully evade the h ...
Cellular localization of the potassium channel Kir7.1 in guinea pig
... Background. K⫹ channels have important functions in the kidney, such as maintenance of the membrane potential, volume regulation, recirculation, and secretion of potassium ions. The aim of this study was to obtain more information on the localization and possible functional role of the inwardly rect ...
... Background. K⫹ channels have important functions in the kidney, such as maintenance of the membrane potential, volume regulation, recirculation, and secretion of potassium ions. The aim of this study was to obtain more information on the localization and possible functional role of the inwardly rect ...
Production of exopolysaccharide from mycelial culture of Grifola
... enzymes target the components of the extracellular matrix (ECM) such as collagen, laminin, fibronectin and proteoglycan [18,19]. The expression of MMPs in UVirradiated fibroblasts is known to be initiated by reactive oxygen species (ROS) and by activation of a cell surface growth factor and cytokine r ...
... enzymes target the components of the extracellular matrix (ECM) such as collagen, laminin, fibronectin and proteoglycan [18,19]. The expression of MMPs in UVirradiated fibroblasts is known to be initiated by reactive oxygen species (ROS) and by activation of a cell surface growth factor and cytokine r ...
Novel Multiprotein Complexes Identified in the Hyperthermophilic
... proteins is used to identify potential complexes. As a model system we use the hyperthermophilic archaeon Pyrococcus furiosus, an anaerobe that grows optimally at 100 °C (28). Its genome sequence (29, 30) contains 2125 ORFs. A universal feature of prokaryotic genomes is the organization of genes int ...
... proteins is used to identify potential complexes. As a model system we use the hyperthermophilic archaeon Pyrococcus furiosus, an anaerobe that grows optimally at 100 °C (28). Its genome sequence (29, 30) contains 2125 ORFs. A universal feature of prokaryotic genomes is the organization of genes int ...
Two glucose/xylose transporter genes from the yeast Candida
... HXT7 promoter was amplified by PCR using plasmid YEpkHXT7 [18] as a template and primers HXT7prom1 (5 -AACCTGCAGCTCGTAGGAACAATTTCGG-3 ) and HXT7prom2 (5 -GGACGGGACATATGCTGATTAAAATTAAAAAAACTT-3 ). At the 5 end these two primers contain restriction sites for PstI and NdeI respectively. The comple ...
... HXT7 promoter was amplified by PCR using plasmid YEpkHXT7 [18] as a template and primers HXT7prom1 (5 -AACCTGCAGCTCGTAGGAACAATTTCGG-3 ) and HXT7prom2 (5 -GGACGGGACATATGCTGATTAAAATTAAAAAAACTT-3 ). At the 5 end these two primers contain restriction sites for PstI and NdeI respectively. The comple ...
Cell-Free Phospholipid Biosynthesis by Gene
... synthesis in E. coli entails two acyltransferase enzymes: the glycerol-3-phosphate (G3P) acyltransferase (GPAT) and the lysophosphatidic acid (LPA) acyltransferase (LPAAT) [8,9]. The enzyme GPAT is an integral membrane protein that uses G3P and either acyl-CoA (CoA, coenzyme A) or acyl-ACP (ACP, acy ...
... synthesis in E. coli entails two acyltransferase enzymes: the glycerol-3-phosphate (G3P) acyltransferase (GPAT) and the lysophosphatidic acid (LPA) acyltransferase (LPAAT) [8,9]. The enzyme GPAT is an integral membrane protein that uses G3P and either acyl-CoA (CoA, coenzyme A) or acyl-ACP (ACP, acy ...
Vaccinia Virus G1L Protein Expression and Purification
... ymxG (Hypothetical zinc protease from B. subtilis) ...
... ymxG (Hypothetical zinc protease from B. subtilis) ...
Production of exopolysaccharide from mycelial culture of Grifola
... enzymes target the components of the extracellular matrix (ECM) such as collagen, laminin, fibronectin and proteoglycan [18,19]. The expression of MMPs in UVirradiated fibroblasts is known to be initiated by reactive oxygen species (ROS) and by activation of a cell surface growth factor and cytokine r ...
... enzymes target the components of the extracellular matrix (ECM) such as collagen, laminin, fibronectin and proteoglycan [18,19]. The expression of MMPs in UVirradiated fibroblasts is known to be initiated by reactive oxygen species (ROS) and by activation of a cell surface growth factor and cytokine r ...
FOG-1 - Blood Journal
... of erythropoiesis, as demonstrated by genetic studies in zebrafish and mice.2-5 GATA-1 null mice showed complete ablation of primitive and definitive erythropoiesis resulting from arrested maturation4,5 and apoptosis of erythroid cells.2 In addition, numerous gain- and loss-of-function experiments s ...
... of erythropoiesis, as demonstrated by genetic studies in zebrafish and mice.2-5 GATA-1 null mice showed complete ablation of primitive and definitive erythropoiesis resulting from arrested maturation4,5 and apoptosis of erythroid cells.2 In addition, numerous gain- and loss-of-function experiments s ...
Amyotrophic lateral sclerosis: Protein
... The disease onset in mice having low mSOD1 expression is delayed as compared to the SOD1G93A mice [20]. Currently, many potential therapeutic agents to treat ALS are tested initially on the SOD1G93A mouse models. Despite the disagreements in therapeutic outcome between animal tests and human clinica ...
... The disease onset in mice having low mSOD1 expression is delayed as compared to the SOD1G93A mice [20]. Currently, many potential therapeutic agents to treat ALS are tested initially on the SOD1G93A mouse models. Despite the disagreements in therapeutic outcome between animal tests and human clinica ...
The regulation of leukemia inhibitory factor
... induced at the mRNA levels under the inflammatory stress, in uterine tissues at the embryonic implantation stage. LIF is also frequently overexpressed at both the mRNA and protein levels in many tumor tissues and tumor environments. The transcriptional regulation of LIF is through different mechanis ...
... induced at the mRNA levels under the inflammatory stress, in uterine tissues at the embryonic implantation stage. LIF is also frequently overexpressed at both the mRNA and protein levels in many tumor tissues and tumor environments. The transcriptional regulation of LIF is through different mechanis ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.