Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for anti-viral therapy.
... underlying biochemistry difficult. An alternative to studying biofluids of affected individuals involves using cellular models to probe disease-associated metabolic changes in a more controlled experimental setting. One class of human disease with useful cellular models is viral infection, as the co ...
... underlying biochemistry difficult. An alternative to studying biofluids of affected individuals involves using cellular models to probe disease-associated metabolic changes in a more controlled experimental setting. One class of human disease with useful cellular models is viral infection, as the co ...
MB206_fhs_lnt_001.1_AT_May09
... metabolism of lactose in Escherichia coli and some other enteric bacteria. It consists of three adjacent structural genes, a promoter, a terminator, and an operator. The lac operon is regulated by several factors including the availability of glucose and of lactose. The regulator does not have to be ...
... metabolism of lactose in Escherichia coli and some other enteric bacteria. It consists of three adjacent structural genes, a promoter, a terminator, and an operator. The lac operon is regulated by several factors including the availability of glucose and of lactose. The regulator does not have to be ...
The Key Enzyme of the Sialic Acid Metabolism Is Involved in
... faster in the absence of Sia. Unfortunately, long-term culture (longer than two days) of embryonic bodies in the absence of Sia is not possible because cells undergo apoptosis in complete serum-free medium (data not shown). Our understanding is that embryoid body formation is more comparable to earl ...
... faster in the absence of Sia. Unfortunately, long-term culture (longer than two days) of embryonic bodies in the absence of Sia is not possible because cells undergo apoptosis in complete serum-free medium (data not shown). Our understanding is that embryoid body formation is more comparable to earl ...
C1qRP Is a Heavily O-Glycosylated Cell Surface Protein Involved in
... receptor. The fact that mAbs R139 and R3 are able to abrogate the enhanced phagocytosis by C1q and MBL indicates that C1qRP is at least a shared, functional component of the receptor that mediates the enhanced phagocytosis induced by these two ligands (4, 13). Using the monoclonal anti-C1qRP to affi ...
... receptor. The fact that mAbs R139 and R3 are able to abrogate the enhanced phagocytosis by C1q and MBL indicates that C1qRP is at least a shared, functional component of the receptor that mediates the enhanced phagocytosis induced by these two ligands (4, 13). Using the monoclonal anti-C1qRP to affi ...
Ancestral lipid biosynthesis and early membrane evolution
... last universal common ancestor (cenancestor) is subject to debate. G1PDH and G3PDH belong to two separate superfamilies that are universally distributed, suggesting that members of both superfamilies existed in the cenancestor. Furthermore, archaea possess homologues to known bacterial genes involve ...
... last universal common ancestor (cenancestor) is subject to debate. G1PDH and G3PDH belong to two separate superfamilies that are universally distributed, suggesting that members of both superfamilies existed in the cenancestor. Furthermore, archaea possess homologues to known bacterial genes involve ...
Method to protect a targeted amino acid residue during random mutagenesis
... Random mutagenesis methods are frequently biased. In PCR mutagenesis, mutations of the type T/A-to-x occur far more often than others (3,4). Thus, the frequency of mutation at F26 (TTT) is expected to be relatively high. Indeed, nine out of 10 sequenced mutants with C40 activity had mutations at thi ...
... Random mutagenesis methods are frequently biased. In PCR mutagenesis, mutations of the type T/A-to-x occur far more often than others (3,4). Thus, the frequency of mutation at F26 (TTT) is expected to be relatively high. Indeed, nine out of 10 sequenced mutants with C40 activity had mutations at thi ...
the Acetyl-Coenzyme A
... bank. This search revealed significant homology to the deduced sequences of the acetyl-CoA synthetases of A. nidulans, N. crassa (Connerton et al., 1990) and Methanothri.~soehrlgenii (Eggen et al., 1991). The ascomycetes A. nidulans and N. crassa showed 78% and 76% similar (conservative replacements ...
... bank. This search revealed significant homology to the deduced sequences of the acetyl-CoA synthetases of A. nidulans, N. crassa (Connerton et al., 1990) and Methanothri.~soehrlgenii (Eggen et al., 1991). The ascomycetes A. nidulans and N. crassa showed 78% and 76% similar (conservative replacements ...
Robust Genome Editing in Stem Cells Using CRISPR
... attractive tool for large-scale genome engineering in a wide variety of hosts. We have developed various CRISPR-Cas9 formats that can be used to edit genomes in a wide variety of cell types, including stem cells. Using these formats we have achieved greater than 50% target-specific DNA cleavage in m ...
... attractive tool for large-scale genome engineering in a wide variety of hosts. We have developed various CRISPR-Cas9 formats that can be used to edit genomes in a wide variety of cell types, including stem cells. Using these formats we have achieved greater than 50% target-specific DNA cleavage in m ...
Chapter 8
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
PPT - Bioinformatics Research Group at SRI International
... FIGfam Protien Families Within the SEED • FIGfams are an attempt to form sets of proteins performing the same cellular function • FIGfams have end to end homology • FIGfams come from two sources • (1) manually curated Subsystems • (2) “close strains” and “conserved clusters” –Aligning two very simi ...
... FIGfam Protien Families Within the SEED • FIGfams are an attempt to form sets of proteins performing the same cellular function • FIGfams have end to end homology • FIGfams come from two sources • (1) manually curated Subsystems • (2) “close strains” and “conserved clusters” –Aligning two very simi ...
Hematopoesis
... communicate useful descriptive information in some circumstances but they have no formally recognized taxonomic meaning’ (8th report, p4) ...
... communicate useful descriptive information in some circumstances but they have no formally recognized taxonomic meaning’ (8th report, p4) ...
GENE MUTATIONS - The Open Door Web Site : Home Page
... Pro-Glu-Glu-Cys-Gly Amino acids © 2010 Paul Billiet ODWS ...
... Pro-Glu-Glu-Cys-Gly Amino acids © 2010 Paul Billiet ODWS ...
Trends in Plant Science
... has generated new insights into how this and later in allelism tests were found to have (Ref. 13). Other research groups have obtained important plant defense response is regulated3. mutations in the same gene, hereafter called similar results, although the specific bZIP proNIM1/NPR1. Subsequent wor ...
... has generated new insights into how this and later in allelism tests were found to have (Ref. 13). Other research groups have obtained important plant defense response is regulated3. mutations in the same gene, hereafter called similar results, although the specific bZIP proNIM1/NPR1. Subsequent wor ...
The Family of SMF Metal Ion Transporters in Yeast Cells*
... encodes a hydrophobic protein of 63,258 Da with potentially eight to ten transmembrane domains. A search in the yeast genome data base with the Smf1p sequence revealed two homologous genes that were named SMF2 and SMF3. These genes encode proteins of 59,758 Da and 51,778 Da, respectively, and contai ...
... encodes a hydrophobic protein of 63,258 Da with potentially eight to ten transmembrane domains. A search in the yeast genome data base with the Smf1p sequence revealed two homologous genes that were named SMF2 and SMF3. These genes encode proteins of 59,758 Da and 51,778 Da, respectively, and contai ...
Assembly, Function, and Dynamics of the
... for studying chloroplast gene expression, especially when it is combined with classical genetic analysis. It has been possible to dissect regulatory elements such as promoters and 5⬘ and 3⬘ untranslated regions, and to use chimeric genes consisting of chloroplast regulatory elements fused to reporte ...
... for studying chloroplast gene expression, especially when it is combined with classical genetic analysis. It has been possible to dissect regulatory elements such as promoters and 5⬘ and 3⬘ untranslated regions, and to use chimeric genes consisting of chloroplast regulatory elements fused to reporte ...
oL-Amylase of Clostridium thermosulfurogenes EMi:
... enzymatically functioning regions. The tentative Ca2+-binding site (consensus region I) of this Ca2+independent enzyme showed only limited homology. The deduced amino acid sequence of a second obviously truncated open reading frame showed significant homology to the malG gene product of E. coli. Com ...
... enzymatically functioning regions. The tentative Ca2+-binding site (consensus region I) of this Ca2+independent enzyme showed only limited homology. The deduced amino acid sequence of a second obviously truncated open reading frame showed significant homology to the malG gene product of E. coli. Com ...
science - Madison Central High
... They often sought to achieve racial purity and supremacy Thomas Morgan showed that that environmental factors could alter fruit fly genes His experiments undermined eugenicists' strict focus on "nature" rather than "nurture" Most scientists discredited eugenics after World War II ...
... They often sought to achieve racial purity and supremacy Thomas Morgan showed that that environmental factors could alter fruit fly genes His experiments undermined eugenicists' strict focus on "nature" rather than "nurture" Most scientists discredited eugenics after World War II ...
Slide 1
... These fragments were combined with vectors to create recombinant DNA, cloned to make many copies, and sequenced using automated sequencing machines. Computers analyzed the overlapping regions to generate one continuous sequence. ...
... These fragments were combined with vectors to create recombinant DNA, cloned to make many copies, and sequenced using automated sequencing machines. Computers analyzed the overlapping regions to generate one continuous sequence. ...
GrpL, a Grb2-related Adaptor Protein, Interacts with SLP-76 to
... One connection between the TCR and SLP-76 may be provided by Grb2 (24). The SH2 domain of Grb2 binds to both Shc and LAT/pp36/38, allowing Grb2 to be localized to the plasma membrane where most of the TCR-activated PTKs are (13, 14, 25). The SH3 domain(s) of Grb2 bind to Sos1 and Sos2 (GEF of the Ra ...
... One connection between the TCR and SLP-76 may be provided by Grb2 (24). The SH2 domain of Grb2 binds to both Shc and LAT/pp36/38, allowing Grb2 to be localized to the plasma membrane where most of the TCR-activated PTKs are (13, 14, 25). The SH3 domain(s) of Grb2 bind to Sos1 and Sos2 (GEF of the Ra ...
by plasmid
... the 5’ ends. They might use some sort of slippage mechanism, using the terminal protein as either a recombinase or a primer or both. ...
... the 5’ ends. They might use some sort of slippage mechanism, using the terminal protein as either a recombinase or a primer or both. ...
Viral tropism - UK-CAB
... Viral tropism is important for a new class of drugs called CCR5 agonists. These are a kind of entry inhibitor (like T-20) and stop HIV binding to cells by blocking the CCR5 coreceptor. ...
... Viral tropism is important for a new class of drugs called CCR5 agonists. These are a kind of entry inhibitor (like T-20) and stop HIV binding to cells by blocking the CCR5 coreceptor. ...
tryptophan operon - Biology Notes Help
... Under severe tryptophan starvation trp genes are expressed maximally and controlled by attenuation. This is accomplished by a mechanism that controls the ...
... Under severe tryptophan starvation trp genes are expressed maximally and controlled by attenuation. This is accomplished by a mechanism that controls the ...
Immunophilins and Parvulins. Superfamily of
... to indicate Mr. Nevertheless, we retained the previously published names for comparison as shown in Table I. Immunophilins consist of two families of proteins, FKBPs and cyclophilins, that show little sequence similarity between the members of the two families. In addition, the overall sequence simi ...
... to indicate Mr. Nevertheless, we retained the previously published names for comparison as shown in Table I. Immunophilins consist of two families of proteins, FKBPs and cyclophilins, that show little sequence similarity between the members of the two families. In addition, the overall sequence simi ...
Document
... Member signature databases Similar coverage in size; Different content Member Database PFAM PROSITE ...
... Member signature databases Similar coverage in size; Different content Member Database PFAM PROSITE ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.