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... unpredictable, pleiotropic effects. This makes the engineering of specific single, desirable traits more complicated than the random transformation attempts in which any new, unusual colour is deemed a success. ...
... unpredictable, pleiotropic effects. This makes the engineering of specific single, desirable traits more complicated than the random transformation attempts in which any new, unusual colour is deemed a success. ...
Linköping University Post Print Distinct parts of leukotriene C-4 synthase
... GST pull-down experiments were performed using GST-fusion proteins of full length LTC4S and seven truncated variants (1-58, 1-88, 1-115, 23-150, 57-150, 87-150, and 114-150), full length FLAP and the three truncated variants (1-51, 47-119, 107-161), and full length 5-LO. The results showed faint int ...
... GST pull-down experiments were performed using GST-fusion proteins of full length LTC4S and seven truncated variants (1-58, 1-88, 1-115, 23-150, 57-150, 87-150, and 114-150), full length FLAP and the three truncated variants (1-51, 47-119, 107-161), and full length 5-LO. The results showed faint int ...
Fredrik Lysholm Bioinformatic methods for characterization of viral pathogens in metagenomic samples Linköping studies in science and technology
... virology. Fueled by the reduction in sequencing costs and the unbiased nature of shotgun sequencing, viral metagenomics has rapidly become the strategy of choice for this exploration. This thesis mainly focuses on improving key methods used in viral metagenomics as well as the complete viral charact ...
... virology. Fueled by the reduction in sequencing costs and the unbiased nature of shotgun sequencing, viral metagenomics has rapidly become the strategy of choice for this exploration. This thesis mainly focuses on improving key methods used in viral metagenomics as well as the complete viral charact ...
Hy-Line - LGC Group
... that variants in the gene may be relevant to the selection of commercial egg-laying poultry lines. In this study (Fulton et al., 2012), exons 2-6 of the OCX32 gene were sequenced in multiple elite commercial egg-laying lines, and SNP detection and analysis carried out using LGC’s KASPTM genotyping c ...
... that variants in the gene may be relevant to the selection of commercial egg-laying poultry lines. In this study (Fulton et al., 2012), exons 2-6 of the OCX32 gene were sequenced in multiple elite commercial egg-laying lines, and SNP detection and analysis carried out using LGC’s KASPTM genotyping c ...
Chapter 43: Plant Genomics
... tool for analyzing the evolutionary patterns of plant species. dispersed among single-copy DNA in the same orientaPlant cells may also contain excess DNA in their tion (“repeat/single-copy interspersion”) or the opposite genomes in the form of highly repetitive sequences, or orientation (“inverted r ...
... tool for analyzing the evolutionary patterns of plant species. dispersed among single-copy DNA in the same orientaPlant cells may also contain excess DNA in their tion (“repeat/single-copy interspersion”) or the opposite genomes in the form of highly repetitive sequences, or orientation (“inverted r ...
BMC Evolutionary Biology D- and L-lactate dehydrogenases during invertebrate evolution
... LDH-A and LDH-B isozymes separating into distinct groups. Even so, there are examples of species whose A, B and C isozymes cluster with one another (e.g. Xenopus laevis). The arthropod LDH sequences form a well-resolved cluster, as do the insects within it. Two copies of Ldh were found in the Daphni ...
... LDH-A and LDH-B isozymes separating into distinct groups. Even so, there are examples of species whose A, B and C isozymes cluster with one another (e.g. Xenopus laevis). The arthropod LDH sequences form a well-resolved cluster, as do the insects within it. Two copies of Ldh were found in the Daphni ...
v11a32-hawse pgmkr
... epithelial and fiber cells, we have analyzed the global gene expression profiles of these two cell types in the human lens. This analysis will also reveal those genes that are exclusively expressed in the epithelial and cortical fiber cells and those genes that may play important roles in the differ ...
... epithelial and fiber cells, we have analyzed the global gene expression profiles of these two cell types in the human lens. This analysis will also reveal those genes that are exclusively expressed in the epithelial and cortical fiber cells and those genes that may play important roles in the differ ...
Module Document
... add to the often-extreme temperatures that these organisms already have to endure, and they might not be able to withstand them. Temperature cues are also important for development and reproduction of many animals. From 1993-1996, researchers at Hopkins Marine Station in California surveyed transect ...
... add to the often-extreme temperatures that these organisms already have to endure, and they might not be able to withstand them. Temperature cues are also important for development and reproduction of many animals. From 1993-1996, researchers at Hopkins Marine Station in California surveyed transect ...
SUMMARY Evidence 1s summarized showing that thymine methyls
... A third reason may have to do with gene expression. That is, thymine methyls may have Increased or decreased the ability of enzymes and/or regulatory proteins to bind to specific sequences. An example 1s the lac repressor which binds nonspecifically to poly(dA-dll) with 20-fold greater affinity than ...
... A third reason may have to do with gene expression. That is, thymine methyls may have Increased or decreased the ability of enzymes and/or regulatory proteins to bind to specific sequences. An example 1s the lac repressor which binds nonspecifically to poly(dA-dll) with 20-fold greater affinity than ...
Influence of genomic G+ C content on average amino
... (-5.4%), and Gly (+5.3%) in IMP, and for Ala (+9.3%), Lys ( - 8.6%), Asn (-6.2%), Arg (+6.0%) and Ile (-5.8%) in non-IMP. For these amino acids, the absolute variation of their frequencies is similar to their average content in proteins so that their relative variation is close to 100%. Since most p ...
... (-5.4%), and Gly (+5.3%) in IMP, and for Ala (+9.3%), Lys ( - 8.6%), Asn (-6.2%), Arg (+6.0%) and Ile (-5.8%) in non-IMP. For these amino acids, the absolute variation of their frequencies is similar to their average content in proteins so that their relative variation is close to 100%. Since most p ...
Production of industrially relevant compounds in prokaryotic
... Zobacler radioresislens catechol 1,2-dioxygenase A subunit. [0031] SEQ ID NOs: 14 and 15 are the nucleic acid and amino acid sequences, respectively, of an exemplary Acine Zobacler radioresislens catechol 1,2-dioxygenase B subunit. [0032] SEQ ID NOs: 16 and 17 are the nucleic acid and amino acid seq ...
... Zobacler radioresislens catechol 1,2-dioxygenase A subunit. [0031] SEQ ID NOs: 14 and 15 are the nucleic acid and amino acid sequences, respectively, of an exemplary Acine Zobacler radioresislens catechol 1,2-dioxygenase B subunit. [0032] SEQ ID NOs: 16 and 17 are the nucleic acid and amino acid seq ...
blast
... is to align the entire sequence against all sequences in a database • This approach is very slow and hence impractical ...
... is to align the entire sequence against all sequences in a database • This approach is very slow and hence impractical ...
The nucleotide sequence of the gene encoding the attachment
... this enables the polymerase to attenuate differentially at this intergenic sequence, explaining the extreme level of attenuation of transcription observed at the H/L intergenic boundary of MV (Cattaneo et al., 1987). The completion of the remaining CDV gene boundary sequences should allow a more com ...
... this enables the polymerase to attenuate differentially at this intergenic sequence, explaining the extreme level of attenuation of transcription observed at the H/L intergenic boundary of MV (Cattaneo et al., 1987). The completion of the remaining CDV gene boundary sequences should allow a more com ...
NDC1 : A Nuclear Periphery Component Required for Yeast Spindle Pole Body Duplication.
... lacking any spindle microtubules, the defective SPB is segregated away from the functional SPB by a mechanism that remains unknown. The ndc/-1 (nuclear division cycle) mutation renders yeast cold-sensitive for growth and causes several defects that are similar to those observed in raps2 mutants, yet ...
... lacking any spindle microtubules, the defective SPB is segregated away from the functional SPB by a mechanism that remains unknown. The ndc/-1 (nuclear division cycle) mutation renders yeast cold-sensitive for growth and causes several defects that are similar to those observed in raps2 mutants, yet ...
The serC-aroA operon of Escherichia coli
... remain capable of complementing an auxotrophic aroA mutation, expression of aroA is reduced. DNA sequence analysis revealed that a sequence approx. 1200 base pairs (bp) upstream of aroA is necessary for its expression. An open reading frame was identified in this region which encodes a protein of 36 ...
... remain capable of complementing an auxotrophic aroA mutation, expression of aroA is reduced. DNA sequence analysis revealed that a sequence approx. 1200 base pairs (bp) upstream of aroA is necessary for its expression. An open reading frame was identified in this region which encodes a protein of 36 ...
Somatotropic axis
... – Affects amount of steroidogenic enzymes • Gender-specific pattern of steroidogenesis ...
... – Affects amount of steroidogenic enzymes • Gender-specific pattern of steroidogenesis ...
SNPs and Haplotypes
... ("snip") is a single base mutation in DNA. • SNPs are ‘conserved’ across the genome, often in patterns called ‘haplotype blocks’ • SNPs are the most simple form and most common source of genetic polymorphism in the human genome (90% of all human DNA polymorphisms are associated with SNPs). ...
... ("snip") is a single base mutation in DNA. • SNPs are ‘conserved’ across the genome, often in patterns called ‘haplotype blocks’ • SNPs are the most simple form and most common source of genetic polymorphism in the human genome (90% of all human DNA polymorphisms are associated with SNPs). ...
Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA
... shows regions of homology throughout the proteins (not shown). However, without any information regarding the tertiary structure of these enzymes, it is di¤cult to make any kind of observations with respect to function based on the homology or lack thereof in particular regions of primary amino acid ...
... shows regions of homology throughout the proteins (not shown). However, without any information regarding the tertiary structure of these enzymes, it is di¤cult to make any kind of observations with respect to function based on the homology or lack thereof in particular regions of primary amino acid ...
Putrescine oxidase of Micrococcus rubens : primary
... contained BamHI fragments in the size range 4 . 2 4 5 kb, DNA was extracted by the sodium perchlorate method (Chen & Thomas, 1980) and ligated with T4 DNA ligase to BamHI-digested pUC19. The ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transforma ...
... contained BamHI fragments in the size range 4 . 2 4 5 kb, DNA was extracted by the sodium perchlorate method (Chen & Thomas, 1980) and ligated with T4 DNA ligase to BamHI-digested pUC19. The ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transforma ...
This article was published in an Elsevier journal. The attached copy
... Fig. 1. Generating ORF deletion mutants (ORFD). (A) The E. coli DY380 strain provides a highly efficient homologous recombination system, which allows recombination of homologous sequences as short as 40 bp. The homologous recombination system is strictly regulated by a temperature sensitive repress ...
... Fig. 1. Generating ORF deletion mutants (ORFD). (A) The E. coli DY380 strain provides a highly efficient homologous recombination system, which allows recombination of homologous sequences as short as 40 bp. The homologous recombination system is strictly regulated by a temperature sensitive repress ...
Gene Section TMPRSS2 (transmembrane protease, serine 2) Atlas of Genetics and Cytogenetics
... domains, TMPRSS2 could work as a receptor for specific ligand(s) mediating signals between the environment and the cell. TMPRSS2 has been proposed to regulate epithelial sodium currents in the lung through proteolytic cleavage of the epithelial sodium channel and inflammatory responses in the prosta ...
... domains, TMPRSS2 could work as a receptor for specific ligand(s) mediating signals between the environment and the cell. TMPRSS2 has been proposed to regulate epithelial sodium currents in the lung through proteolytic cleavage of the epithelial sodium channel and inflammatory responses in the prosta ...
Characterisation of the diol dehydratase pdu operon of Lactobacillus
... two ORFs in the downstream region (pduGH). The deduced amino acid sequences from pduA and pduB exhibited extensive homology with the corresponding genes of S. enterica (Table 2). PduG, located 15 bp downstream of the stop codon of the ORF pduE, could encode a large subunit of the reactivating factor ...
... two ORFs in the downstream region (pduGH). The deduced amino acid sequences from pduA and pduB exhibited extensive homology with the corresponding genes of S. enterica (Table 2). PduG, located 15 bp downstream of the stop codon of the ORF pduE, could encode a large subunit of the reactivating factor ...
Analysis - The Journal of Cell Biology
... where multiple kinases and phosphatases contribute to the outcome of a pathway. To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. A variety of approaches, including b ...
... where multiple kinases and phosphatases contribute to the outcome of a pathway. To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. A variety of approaches, including b ...
Cardiac Energy Dependence on Glucose Increases
... towards the synthesis of complex lipids.9 Consistent with the switch to glucose metabolism in Acsl1H/ hearts, uptake of the FA analog 2-Br[14C]palmitate decreases 35%, while uptake of 2-deoxy[14C]glucose increases 8-fold.9,10 Although one might predict that Acsl1H/ hearts have low energy stores, ...
... towards the synthesis of complex lipids.9 Consistent with the switch to glucose metabolism in Acsl1H/ hearts, uptake of the FA analog 2-Br[14C]palmitate decreases 35%, while uptake of 2-deoxy[14C]glucose increases 8-fold.9,10 Although one might predict that Acsl1H/ hearts have low energy stores, ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.