Ch44-The Biochemistry of the Erythrocyte and Other Blood Cells
... The cells of the blood are classified as erythrocytes, leukocytes, or thrombocytes. The erythrocytes (red cells) carry oxygen to the tissues and are the most numerous cells in the blood. The leukocytes (white cells) are involved in defense against infection, and the thrombocytes (platelets) function ...
... The cells of the blood are classified as erythrocytes, leukocytes, or thrombocytes. The erythrocytes (red cells) carry oxygen to the tissues and are the most numerous cells in the blood. The leukocytes (white cells) are involved in defense against infection, and the thrombocytes (platelets) function ...
Chapter 1: Biochemistry in the Modern World
... organisms rather than cells. In ecology, for example, ecosystems are often described in terms of food webs, with energy being generated by photosynthesis and then transferred up a food chain through the herbivores to the top carnivores (Fig. 1.2). The generation of energy is a central topic in bioch ...
... organisms rather than cells. In ecology, for example, ecosystems are often described in terms of food webs, with energy being generated by photosynthesis and then transferred up a food chain through the herbivores to the top carnivores (Fig. 1.2). The generation of energy is a central topic in bioch ...
Comparative genomic analysis of carbon and nitrogen assimilation
... independent transcriptional terminators in the interoperon regions, but in any of the 4 operons an evident terminator was found. Nevertheless the inter-operon regions in both cases is big enough for not to suspect a cotranscriptions of genes as it is probed by RT-PCR results (474 nts between ccs and ...
... independent transcriptional terminators in the interoperon regions, but in any of the 4 operons an evident terminator was found. Nevertheless the inter-operon regions in both cases is big enough for not to suspect a cotranscriptions of genes as it is probed by RT-PCR results (474 nts between ccs and ...
Addition of a photocrosslinking amino acid to the genetic code of
... reporter strain in which the production of full-length CAT and T7 RNA polymerase depend on suppression of amber stop codons in the CAT and T7 RNA polymerase gene, respectively (S. W. Santoro and P.G.S., unpublished data). Because the T7 RNA polymerase drives expression of the GFP these cells can be ...
... reporter strain in which the production of full-length CAT and T7 RNA polymerase depend on suppression of amber stop codons in the CAT and T7 RNA polymerase gene, respectively (S. W. Santoro and P.G.S., unpublished data). Because the T7 RNA polymerase drives expression of the GFP these cells can be ...
calicin (S-13): sc-162627 - Santa Cruz Biotechnology
... contributes to the rigid structure of the calyx. calicin may function as a morphogenetic cytoskeletal element during spermiogenic differentiation, and the absence or deformation of calicin may contribute to sperm malformations such as teratozoospermia. Containing one BACK (BTB/Kelch associated) doma ...
... contributes to the rigid structure of the calyx. calicin may function as a morphogenetic cytoskeletal element during spermiogenic differentiation, and the absence or deformation of calicin may contribute to sperm malformations such as teratozoospermia. Containing one BACK (BTB/Kelch associated) doma ...
Document
... been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
... been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
Isolation and Characterization of a Cytochrome P450 Gene from
... To determine the complexity of the MaP450 gene family in the genome of M. armeniacum ‘Blue Pearl’, Southern hybridization was carried out using a 1.67-kb DNA fragment from MaP450 as the probe (Fig. 3). Five or six bands were observed for each restriction enzyme, indicating the presence of a gene fam ...
... To determine the complexity of the MaP450 gene family in the genome of M. armeniacum ‘Blue Pearl’, Southern hybridization was carried out using a 1.67-kb DNA fragment from MaP450 as the probe (Fig. 3). Five or six bands were observed for each restriction enzyme, indicating the presence of a gene fam ...
De Novo Mutation Found in the Porphobilinogen Deaminase Gene
... fragment containing the complete encoding region. This fragment was inserted into prokaryotic expression vector. The cells of recombinant strain E. coli BL21 were transformed by this construct. By side-directed mutagenesis we introduced desired mutation into the construct. The accuracy and absence o ...
... fragment containing the complete encoding region. This fragment was inserted into prokaryotic expression vector. The cells of recombinant strain E. coli BL21 were transformed by this construct. By side-directed mutagenesis we introduced desired mutation into the construct. The accuracy and absence o ...
Current Topics in the Biology of Disease CH400
... ‘suicide genes’ or ‘death genes’. • Since the early 80’s a number of suicide genes and anti-suicide genes have been identified. • A number of these apoptosis regulating genes turned out to be oncogenes e.g. bcl-2, p53 and c-myc. ...
... ‘suicide genes’ or ‘death genes’. • Since the early 80’s a number of suicide genes and anti-suicide genes have been identified. • A number of these apoptosis regulating genes turned out to be oncogenes e.g. bcl-2, p53 and c-myc. ...
Gene Section FIGF (c fos induced growth factor (vascular endothelial growth factor D))
... Flk1), VEGFR3 (Flt4), neuropilin-1 and neuropilin-2. VEGFA binds to VEGFR1, VEGFR2, as well as neuropilin-1 and neuropilin-2, whereas PlGF and VEGFB bind only to VEGFR1 and neuropilin-1. VEGF-C and VEGF-D, which share 23% amino acid sequence identity, are uniquely expressed as preproproteins that co ...
... Flk1), VEGFR3 (Flt4), neuropilin-1 and neuropilin-2. VEGFA binds to VEGFR1, VEGFR2, as well as neuropilin-1 and neuropilin-2, whereas PlGF and VEGFB bind only to VEGFR1 and neuropilin-1. VEGF-C and VEGF-D, which share 23% amino acid sequence identity, are uniquely expressed as preproproteins that co ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Exploring the Effects of Human Cytomegalovirus Infection on Host
... There are several antivirals approved to treat HCMV currently on the market, but viral strains resistant to all of the existing drugs have been reported. Since all of the drugs target some aspect of nucleic acid metabolism, future antiviral development could benefit from targeting other aspects of t ...
... There are several antivirals approved to treat HCMV currently on the market, but viral strains resistant to all of the existing drugs have been reported. Since all of the drugs target some aspect of nucleic acid metabolism, future antiviral development could benefit from targeting other aspects of t ...
Quick Overview of Bioinformatics - What's New?
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
Quick Overview of Bioinformatics
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
... Remove errors Compare with database of ‘known function proteins’ • Provide transitive annotations ...
An Upstream Enhancer Region Located at -1810/
... significant increase in luciferase values for both lines with increased ovulation rates, Meishan and Index, over that of Control. Reduction of the proximal promoter from 5118 to 1900 bp relative to the translational start site maintained a 2-4 fold increase in luciferase activity (Index and Meishan, ...
... significant increase in luciferase values for both lines with increased ovulation rates, Meishan and Index, over that of Control. Reduction of the proximal promoter from 5118 to 1900 bp relative to the translational start site maintained a 2-4 fold increase in luciferase activity (Index and Meishan, ...
Molecular cloning and expression of the male sterility - Funpec-RP
... Phosphoglyceraldehyde dehydrogenase (GAPDH) was used as internal reference gene, and its primers were synthesized as follows: GAPDH F: 5'-CCGCTAACTGCCTTGCTCCACTT-3' and GAPDH R: 5'-GCGGCTCTTCCACCTCTCCAGT-3'. Real-time PCR amplification was conducted using the fluorescent dye (TaKaRa Company), with c ...
... Phosphoglyceraldehyde dehydrogenase (GAPDH) was used as internal reference gene, and its primers were synthesized as follows: GAPDH F: 5'-CCGCTAACTGCCTTGCTCCACTT-3' and GAPDH R: 5'-GCGGCTCTTCCACCTCTCCAGT-3'. Real-time PCR amplification was conducted using the fluorescent dye (TaKaRa Company), with c ...
Bioinformatic approach to identify penultimate amino acids efficient
... human results. First, although eukaryotes have both MAP1 and MAP2 proteins, MAP1 appears to be the dominant isoform whose loss leads to dramatic decrease in growth whereas loss of MAP2 decreases growth only slightly [25]. In contrast, MAP2 is more important than MAP1 in higher eukaryotes [2]. MAP2 i ...
... human results. First, although eukaryotes have both MAP1 and MAP2 proteins, MAP1 appears to be the dominant isoform whose loss leads to dramatic decrease in growth whereas loss of MAP2 decreases growth only slightly [25]. In contrast, MAP2 is more important than MAP1 in higher eukaryotes [2]. MAP2 i ...
MECP2, CDKL5 and FOXG1
... Rare (<1% of families) Occurs in both males and females No easy, non-invasive test Higher recurrence risk (?%) ...
... Rare (<1% of families) Occurs in both males and females No easy, non-invasive test Higher recurrence risk (?%) ...
Catabolic Plasmids - UQ eSpace
... cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
... cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
Improved Tolerance to Salt and Water Stress in
... increasing rapidly, and since 2000, when the first LEA-like protein was reported in an invertebrate species (Solomon et al., 2000), more than 30 other LEA and LEA-like protein sequences have been deposited into the database of the National Center for Biotechnology Information (NCBI) from animals bel ...
... increasing rapidly, and since 2000, when the first LEA-like protein was reported in an invertebrate species (Solomon et al., 2000), more than 30 other LEA and LEA-like protein sequences have been deposited into the database of the National Center for Biotechnology Information (NCBI) from animals bel ...
gmod-update-07nov - IUBio Archive for Biology
... • CV: Controlled vocabularies and ontologies • Sequence: Biological sequences and objects which can be localized on them • Companalysis: Adjunct to sequence module for insilico analysis • Map: Adjunct to sequence module for non-sequence localization ...
... • CV: Controlled vocabularies and ontologies • Sequence: Biological sequences and objects which can be localized on them • Companalysis: Adjunct to sequence module for insilico analysis • Map: Adjunct to sequence module for non-sequence localization ...
Characterizing the Grape Transcriptome
... Ontology categories. A simple structured vocabulary, with modules for plant genotype, plant development, and stress, was developed to describe the relationship between individual expressed sequence tags and cDNA libraries; the resulting vocabulary provides query terms to facilitate data mining withi ...
... Ontology categories. A simple structured vocabulary, with modules for plant genotype, plant development, and stress, was developed to describe the relationship between individual expressed sequence tags and cDNA libraries; the resulting vocabulary provides query terms to facilitate data mining withi ...
AIBSTCT Nucleic Acids Research - Walter Lab
... A radiolabelled Pstl-Hindlll fragment of the partial clone p19.8 selected by antibody screening was used to screen a human liver cDNA library cloned into bacteriophage lambda NM1 149. This library was kindly provided by C. Schneider. The cDNA library was plated out in Escherichia coli Q358 cells. In ...
... A radiolabelled Pstl-Hindlll fragment of the partial clone p19.8 selected by antibody screening was used to screen a human liver cDNA library cloned into bacteriophage lambda NM1 149. This library was kindly provided by C. Schneider. The cDNA library was plated out in Escherichia coli Q358 cells. In ...
Many transcription factors recognize DNA shape
... alter binding sites of structural proteins and chromatin modifiers. Approach: CRISPR edit sites identified by TargetFinder, then test chromatin and expression. ...
... alter binding sites of structural proteins and chromatin modifiers. Approach: CRISPR edit sites identified by TargetFinder, then test chromatin and expression. ...
An acyltransferase-like gene obtained by differential gene
... genomic PCR was performed by using the genomic DNA extracted from young leaves of both bitter and sweet cultivars of L. angustifolius (Qiagen DNeasy Plant Mini Kit). Genomic PCR was carried out using Ex Taq DNA polymerase (TaKaRa) and the same specific primers used for semi-quantitative RT-PCR. The ...
... genomic PCR was performed by using the genomic DNA extracted from young leaves of both bitter and sweet cultivars of L. angustifolius (Qiagen DNeasy Plant Mini Kit). Genomic PCR was carried out using Ex Taq DNA polymerase (TaKaRa) and the same specific primers used for semi-quantitative RT-PCR. The ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.