Highly Efficient Micro RNA Enrichment
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
Functional analysis of the human CDC5L complex
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
Translocation of proteins across archaeal cytoplasmic membranes
... of the flagellar subunit is likely to play a role in assembly similar to that in bacterial pili, it is not clear why the sugar-binding proteins retain their hydrophobic core. 2.1.2. Post-translational targeting Post-translational translocation, or translocation of a protein after at least a portion o ...
... of the flagellar subunit is likely to play a role in assembly similar to that in bacterial pili, it is not clear why the sugar-binding proteins retain their hydrophobic core. 2.1.2. Post-translational targeting Post-translational translocation, or translocation of a protein after at least a portion o ...
HnRNP C1/C2 May Regulate Exon 7 Splicing in the Spinal Muscular
... which leads to the axial and limb weakness associated with muscle atrophy. SMA is caused by the homologous deletion and/or deleterious mutation of the SMN1 gene, which encodes the protein SMN (15). SMN is a component of nuclear structures known as gems, suggesting a role in RNA metabolism (7, 16, 20 ...
... which leads to the axial and limb weakness associated with muscle atrophy. SMA is caused by the homologous deletion and/or deleterious mutation of the SMN1 gene, which encodes the protein SMN (15). SMN is a component of nuclear structures known as gems, suggesting a role in RNA metabolism (7, 16, 20 ...
Can the Origin of the Genetic Code Be Explained - BIO
... world] must also show chemical selectivity (or there will be no coding)” [11]. Yarus et al. posit an early state in which RNA molecules with certain base sequences differentially attract the particular amino acids with which they are associated in the modern genetic code – thus enabling the code to ...
... world] must also show chemical selectivity (or there will be no coding)” [11]. Yarus et al. posit an early state in which RNA molecules with certain base sequences differentially attract the particular amino acids with which they are associated in the modern genetic code – thus enabling the code to ...
Isolation and Characterization of Foaming Proteins of
... precipitation and ion-exchange chromatography on diethylaminoethylcellulose. Because the content of foaming proteins correlated well with head formation of many samples of beer, these foaming proteins seem to be responsible for beer foaming. Foaming proteins consisted of three fractions with molecul ...
... precipitation and ion-exchange chromatography on diethylaminoethylcellulose. Because the content of foaming proteins correlated well with head formation of many samples of beer, these foaming proteins seem to be responsible for beer foaming. Foaming proteins consisted of three fractions with molecul ...
Gene expression control by selective RNA processing and
... the term (1) ‘RNA maturation’ to refer to a specific regulatory cleavage, which usually occurs within the 5′ transcript region and changes a transcript to a biologically active RNA molecule, and (2) ‘RNA degradation’ for a cleavage triggering RNA decay. For example, it is wellknown that ribosomal an ...
... the term (1) ‘RNA maturation’ to refer to a specific regulatory cleavage, which usually occurs within the 5′ transcript region and changes a transcript to a biologically active RNA molecule, and (2) ‘RNA degradation’ for a cleavage triggering RNA decay. For example, it is wellknown that ribosomal an ...
Use of Cellular Decapping Activators by Positive
... West Nile virus (WNV) and Chikungunya virus (CHIKV). Despite their diversity in terms of genome organization or virion morphology, the replication cycle of (+)RNA viruses is highly conserved [1]. Upon entering the target cell and gaining access to the cytoplasm, their single-stranded RNA genomes act ...
... West Nile virus (WNV) and Chikungunya virus (CHIKV). Despite their diversity in terms of genome organization or virion morphology, the replication cycle of (+)RNA viruses is highly conserved [1]. Upon entering the target cell and gaining access to the cytoplasm, their single-stranded RNA genomes act ...
Free amino acids and proteins dynamics in somatic embryogenesis
... acids represent the first step in nitrogen assimilation (Ortiz-Lopez et al., 2000). In MS/2 medium, free amino acids contents was low in embryogenic calli and then increased significantly during globular and bipolar embryos formation. These results are similar to those obtained in Pinus patula (Mala ...
... acids represent the first step in nitrogen assimilation (Ortiz-Lopez et al., 2000). In MS/2 medium, free amino acids contents was low in embryogenic calli and then increased significantly during globular and bipolar embryos formation. These results are similar to those obtained in Pinus patula (Mala ...
Comparative analyses of Saccharomyces cerevisiae RNAs using
... from a limited number of cells picked up from speci¢c ...
... from a limited number of cells picked up from speci¢c ...
Evolution of hard proteins in the sauropsid integument in relation to
... Fig. 2 Drawing illustrating the embryonic layers formed on the scale of embryonic crocodilians (A) in comparison with those of bird embryos (B). The arrowed square illustrates the sequence of layers in crocodilian scale (A1) vs. avian beak (C), downfeather (D), claw (E), and scutate scales (F). The ...
... Fig. 2 Drawing illustrating the embryonic layers formed on the scale of embryonic crocodilians (A) in comparison with those of bird embryos (B). The arrowed square illustrates the sequence of layers in crocodilian scale (A1) vs. avian beak (C), downfeather (D), claw (E), and scutate scales (F). The ...
RNA Isolation and Technology Applications
... The RNA target and RNA probe are hybridized in solution Following hybridization, the RNA is digested with RNases specific for single-stranded nucleic acids – Any remaining unhybridized single-stranded RNA target and probe is degraded – Target:probe hybridized sequences are left intact Following RNas ...
... The RNA target and RNA probe are hybridized in solution Following hybridization, the RNA is digested with RNases specific for single-stranded nucleic acids – Any remaining unhybridized single-stranded RNA target and probe is degraded – Target:probe hybridized sequences are left intact Following RNas ...
Doc S1.
... The PCR reaction profile can be divided in three steps: an early background phase, an exponential phase and a plateau. During the exponential phase the amplification course is described by the equation: Qn Q0 E n , where Qn is the amount of target at cycle n, Q0 is the initial amount of target, ...
... The PCR reaction profile can be divided in three steps: an early background phase, an exponential phase and a plateau. During the exponential phase the amplification course is described by the equation: Qn Q0 E n , where Qn is the amount of target at cycle n, Q0 is the initial amount of target, ...
PDF - SAGE Journals
... requires only CO2 as a carbon source for synthesizing all necessary biomolecules. Changes in the environmental availability of these molecules can be expected to activate regulatory mechanisms manifested as the up and down regulation of specific genes and the concomitant increase and decrease in abu ...
... requires only CO2 as a carbon source for synthesizing all necessary biomolecules. Changes in the environmental availability of these molecules can be expected to activate regulatory mechanisms manifested as the up and down regulation of specific genes and the concomitant increase and decrease in abu ...
WW Domains Provide a Platform for the
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
The SMN Complex Is Associated with snRNPs throughout Their
... and Western blotting. The snRNA export factor PHAX is the earliest detectable marker of newly exported snRNAs in the cytoplasm (49). Phosphorylated PHAX is associated with the snRNAs in the nucleus, and following snRNA export, PHAX is dephosphorylated (49). The anti-SMN antibody specifically coimmun ...
... and Western blotting. The snRNA export factor PHAX is the earliest detectable marker of newly exported snRNAs in the cytoplasm (49). Phosphorylated PHAX is associated with the snRNAs in the nucleus, and following snRNA export, PHAX is dephosphorylated (49). The anti-SMN antibody specifically coimmun ...
OLSON LAB PROTOCOL: Working with RNA
... or substrates for gene translation or regulation. As such, they are expressed in effectively all cells at all times (commonly refered to as ‚housekeeping‘ genes). RNAs for coding genes are called messager RNAs (mRNA) and consist of all of a gene‘s exons spliced together into a contiguous sequence of ...
... or substrates for gene translation or regulation. As such, they are expressed in effectively all cells at all times (commonly refered to as ‚housekeeping‘ genes). RNAs for coding genes are called messager RNAs (mRNA) and consist of all of a gene‘s exons spliced together into a contiguous sequence of ...
Full-Text PDF
... acid residues [33]. The small HPCopP is homologous to CopZ, encoded by the E. hirae and B. subtilis cop operons. CopZ proteins have a conserved structure, β-α-β-β-α-β with a similar metal binding region. However, the structure of HPCopP has a β-α-β-β-α fold (Figure 3A). The helices correspond to res ...
... acid residues [33]. The small HPCopP is homologous to CopZ, encoded by the E. hirae and B. subtilis cop operons. CopZ proteins have a conserved structure, β-α-β-β-α-β with a similar metal binding region. However, the structure of HPCopP has a β-α-β-β-α fold (Figure 3A). The helices correspond to res ...
Appendix A: General Remarks on Handling RNA
... Appendix D: Optional On-Column DNase Digestion with the RNase-Free DNase Set The RNase-Free DNase Set (cat. no. 79254) provides efficient on-column digestion of DNA during RNA purification. The DNase is efficiently removed in subsequent wash steps. Note: Standard DNase buffers are not compatible wit ...
... Appendix D: Optional On-Column DNase Digestion with the RNase-Free DNase Set The RNase-Free DNase Set (cat. no. 79254) provides efficient on-column digestion of DNA during RNA purification. The DNase is efficiently removed in subsequent wash steps. Note: Standard DNase buffers are not compatible wit ...
Fal1p Is an Essential DEAD-Box Protein Involved in 40S
... ATPase activity may require a specific RNA, as shown for the Escherichia coli DbpA protein (19). Thus, most of the putative RNA helicases could exert their in vivo function by modulating the structure of an RNA and thereby regulating its function or its accessibility. Yeast pre-rRNA processing invol ...
... ATPase activity may require a specific RNA, as shown for the Escherichia coli DbpA protein (19). Thus, most of the putative RNA helicases could exert their in vivo function by modulating the structure of an RNA and thereby regulating its function or its accessibility. Yeast pre-rRNA processing invol ...
10858_2015_9967_MOESM1_ESM
... focus on homogeneity effects caused by DMSO and not 2’-O-methylation of the primers. For this study, we chose A/U rich sequences, which caused severe amounts of non-DNA-templated nucleotide addition (>+4). First, we illustrate the effect of DMSO on 3’ end homogeneity on a series of rather short cons ...
... focus on homogeneity effects caused by DMSO and not 2’-O-methylation of the primers. For this study, we chose A/U rich sequences, which caused severe amounts of non-DNA-templated nucleotide addition (>+4). First, we illustrate the effect of DMSO on 3’ end homogeneity on a series of rather short cons ...
NUCLEAR PROTEINS II. Similarity of Nonhistone Proteins in
... Biochem. 4"/:263-270) is a prominent nucleoplasmic protein; (e) quantitation of the major N H P indicates that they are present in a range of 2.2 • 105-5.2 • l06 copies per diploid nucleus. Most of the electrophoretically visible NHP are probably structural rather than regulatory proteins; 09 actin, ...
... Biochem. 4"/:263-270) is a prominent nucleoplasmic protein; (e) quantitation of the major N H P indicates that they are present in a range of 2.2 • 105-5.2 • l06 copies per diploid nucleus. Most of the electrophoretically visible NHP are probably structural rather than regulatory proteins; 09 actin, ...
Lecture 16 - Gene Transcription and Translation
... • Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions • These noncoding regions are called intervening sequences, or introns • The other regions are called exons because they are eventually expressed, usually translated into am ...
... • Most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides that lie between coding regions • These noncoding regions are called intervening sequences, or introns • The other regions are called exons because they are eventually expressed, usually translated into am ...
Vertebrate hairy and Enhancer of split related proteins
... speci®c to bHLH-O proteins (see below). The intervening sequence between the bHLH and Orange domains ranges from seven to 41 amino acids. Although not shown in Figure 2, this intervening sequence is highly similar among members of the hairy, Hey, and Stra13 subfamilies, respectively, while in the E( ...
... speci®c to bHLH-O proteins (see below). The intervening sequence between the bHLH and Orange domains ranges from seven to 41 amino acids. Although not shown in Figure 2, this intervening sequence is highly similar among members of the hairy, Hey, and Stra13 subfamilies, respectively, while in the E( ...
What Does the Microsporidian E. cuniculi Tell Us About the Origin of
... the bare essential cellular functions such as DNA replication, protein synthesis (translation and transcription), metabolism (glycolysis), and various membrane related functions. It was assumed that the minimal bacterial cell would be a minimal cell for the other cellular domains. However, when Mush ...
... the bare essential cellular functions such as DNA replication, protein synthesis (translation and transcription), metabolism (glycolysis), and various membrane related functions. It was assumed that the minimal bacterial cell would be a minimal cell for the other cellular domains. However, when Mush ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.