Ribosomes and Protein Synthesis
... polypeptide polymerization (18, 46) . However, the term microsome is only an operational defmition of a high-speed, multicomponent, sedimentable cell fraction, and soon it became clear that the ribonucleoprotein particles of the microsome fraction are the actual marshalling site for polypeptide poly ...
... polypeptide polymerization (18, 46) . However, the term microsome is only an operational defmition of a high-speed, multicomponent, sedimentable cell fraction, and soon it became clear that the ribonucleoprotein particles of the microsome fraction are the actual marshalling site for polypeptide poly ...
Intracellular Protein Degradation
... While the experimental study involved the bacterial -galactosidase, the conclusions were broader, including also the authors’ hypothesis on mammalian proteins. The use of the term ‘dynamic state’ was not incidental, as they challenged directly Schoenheimer’s studies. Now, after more then six decade ...
... While the experimental study involved the bacterial -galactosidase, the conclusions were broader, including also the authors’ hypothesis on mammalian proteins. The use of the term ‘dynamic state’ was not incidental, as they challenged directly Schoenheimer’s studies. Now, after more then six decade ...
Non-coding RNAs - Biology Department | UNC Chapel Hill
... More than 150 partner proteins are also involved in this process, a subject that has been extensively reviewed10,11. Less well understood is the life history of the snRNPs prior to their assembly into the spliceosome. The recent studies highlighted here indicate that transcription and 3′ processing ...
... More than 150 partner proteins are also involved in this process, a subject that has been extensively reviewed10,11. Less well understood is the life history of the snRNPs prior to their assembly into the spliceosome. The recent studies highlighted here indicate that transcription and 3′ processing ...
intact-protein based sample preparation strategies for
... were isolated from a single frozen section of human breast by LCM, and were subjected to direct MALDI-TOF analysis. Distinct mass spectra were obtained from 1,250 cells from each of the four cell types. The stromal cells revealed several prominent peaks in the 4.5–7.0 kDa range. Those peaks were att ...
... were isolated from a single frozen section of human breast by LCM, and were subjected to direct MALDI-TOF analysis. Distinct mass spectra were obtained from 1,250 cells from each of the four cell types. The stromal cells revealed several prominent peaks in the 4.5–7.0 kDa range. Those peaks were att ...
Repeat proteins challenge the concept of structural domains
... It was early on noted that many natural proteins typically collapse stretches of amino acid chains into compact units, defining structural domains [1]. These domains typically correlate with biological activities and many modern proteins can be described as composed by novel ‘domain arrangements’ [2 ...
... It was early on noted that many natural proteins typically collapse stretches of amino acid chains into compact units, defining structural domains [1]. These domains typically correlate with biological activities and many modern proteins can be described as composed by novel ‘domain arrangements’ [2 ...
Isr J Chem (2010) - Weizmann Institute of Science
... with the universal sequence CCA, to which the cognate amino acid is bound via an ester linkage. These tRNA molecules are the non-ribosomal entities bridging the two subunits, as two of their three binding sites (A- (aminoacyl), P- (peptidyl), and E- (exit) sites) reside on both subunits. At the A- a ...
... with the universal sequence CCA, to which the cognate amino acid is bound via an ester linkage. These tRNA molecules are the non-ribosomal entities bridging the two subunits, as two of their three binding sites (A- (aminoacyl), P- (peptidyl), and E- (exit) sites) reside on both subunits. At the A- a ...
RNA Polymerase - California Lutheran University
... Alternative splicing • Single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons • 15% of known human genetic disorders are due to altered splicing • 35 to 59% of human genes exhibit some form of alternative splicing • Explains how 25,000 genes of the ...
... Alternative splicing • Single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons • 15% of known human genetic disorders are due to altered splicing • 35 to 59% of human genes exhibit some form of alternative splicing • Explains how 25,000 genes of the ...
P1 and P2 protein heterodimer binding to the P0 protein of
... The stalk is a functional domain of the large ribosomal subunit that is directly involved in the interaction and GTPase activity of several soluble factors during translation (1). In eukaryotes, the stalk is formed by a central 32-kDa protein, P0, which interacts through its N-terminal domain (NTD) ...
... The stalk is a functional domain of the large ribosomal subunit that is directly involved in the interaction and GTPase activity of several soluble factors during translation (1). In eukaryotes, the stalk is formed by a central 32-kDa protein, P0, which interacts through its N-terminal domain (NTD) ...
Chapter 15
... to a promoter and to initiate gene expression • Interact with RNA polymerase to form initiation complex at promoter ...
... to a promoter and to initiate gene expression • Interact with RNA polymerase to form initiation complex at promoter ...
Document
... Self-cleavage of the RNA molecules • The regulation of glmS gene(glutamine-fructose-6phosphate amidotransferase)in B. subtilis is by associated activity of ribozyme and riboswitches. • In this case the 5’ UTR of glmS binds with gluosamin6-phosphate, product of GlmS activity and start acting as ribo ...
... Self-cleavage of the RNA molecules • The regulation of glmS gene(glutamine-fructose-6phosphate amidotransferase)in B. subtilis is by associated activity of ribozyme and riboswitches. • In this case the 5’ UTR of glmS binds with gluosamin6-phosphate, product of GlmS activity and start acting as ribo ...
occasional article intracellular protein degradation: from a vague
... nascent lysosomes that have not yet been engaged yet in any proteolytic process; early autophagic vacuoles that might contain intracellular organelles; intermediate/late endosomes and phagocytic vacuoles (heterophagic vacuoles) that contain extracellular contents/particles; and multivesicular bodies ...
... nascent lysosomes that have not yet been engaged yet in any proteolytic process; early autophagic vacuoles that might contain intracellular organelles; intermediate/late endosomes and phagocytic vacuoles (heterophagic vacuoles) that contain extracellular contents/particles; and multivesicular bodies ...
Intrinsically Disordered Proteins (IDPs)
... Proteins (IDPs) and ID Regions (IDRs) • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordi ...
... Proteins (IDPs) and ID Regions (IDRs) • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordi ...
sv-lncs - School of Mathematical and Computer Sciences
... regions is further amplified. There are other functions, such as the density function [11], but these are not evaluated here. SWEMODE has three options concerning traversal of nodes that are considered for inclusion in a module, as described in [16]. In previous work, we applied immediate neighbour ...
... regions is further amplified. There are other functions, such as the density function [11], but these are not evaluated here. SWEMODE has three options concerning traversal of nodes that are considered for inclusion in a module, as described in [16]. In previous work, we applied immediate neighbour ...
Protein Structure Prediction
... The final network yields an overall prediction accuracy of 75.5% when tested by sevenfold cross-validation on a set of 426 non-homologous protein chains. The corresponding Qpred., Qobs. and MCC values are 49.8%, 72.3% and 0.43 respectively and are the best among all the previously published -turn p ...
... The final network yields an overall prediction accuracy of 75.5% when tested by sevenfold cross-validation on a set of 426 non-homologous protein chains. The corresponding Qpred., Qobs. and MCC values are 49.8%, 72.3% and 0.43 respectively and are the best among all the previously published -turn p ...
Tsui, S, Dai, T, Warren, ST and Yen, P: Association of the mouse infertility factor DAZL1 with actively translating polyribosomes. Biology of Reproduction 62:1655-1660 (2000).
... DAZ repeat region. It was proposed that the DAZ genes originated from translocation of an ancestral DAZL1 gene to the Y chromosome, followed by amplification and pruning [10]. A role for DAZ and DAZL1 in spermatogenesis is supported by their exclusive expression in germ cells, their homology to a Dr ...
... DAZ repeat region. It was proposed that the DAZ genes originated from translocation of an ancestral DAZL1 gene to the Y chromosome, followed by amplification and pruning [10]. A role for DAZ and DAZL1 in spermatogenesis is supported by their exclusive expression in germ cells, their homology to a Dr ...
CHAPTER 5 Gene Expression: Transcription
... A nucleotide (18-38 nt upstream of the 3’ spice junction) in the branchpoint sequenc of the intron, forming an RNA lariat structure. • i. in mammals, the branch-point consensus sequence is YNCURAY. • ii. In yeast, the branch point consensus sequence is UACUAAC; its position is more variable than in ...
... A nucleotide (18-38 nt upstream of the 3’ spice junction) in the branchpoint sequenc of the intron, forming an RNA lariat structure. • i. in mammals, the branch-point consensus sequence is YNCURAY. • ii. In yeast, the branch point consensus sequence is UACUAAC; its position is more variable than in ...
Full Text - Molecular Biology and Evolution
... decreases to 2.4 in unicellular eukaryotes, and to 1.7 in multicellular eukaryotes (fig. 3A; one-way analysis of variance: F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While proka ...
... decreases to 2.4 in unicellular eukaryotes, and to 1.7 in multicellular eukaryotes (fig. 3A; one-way analysis of variance: F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While proka ...
Press Release
... lead to a completely new understanding of how mRNA and ribosomes interact.” Proteins are produced from mRNA by ribosomes – ‘molecular machines’ that pass successively along the mRNA to translate its nucleotides into amino acids. It was thought that the mRNA only started to decay once the final ribos ...
... lead to a completely new understanding of how mRNA and ribosomes interact.” Proteins are produced from mRNA by ribosomes – ‘molecular machines’ that pass successively along the mRNA to translate its nucleotides into amino acids. It was thought that the mRNA only started to decay once the final ribos ...
april7_maindocument_jvirol
... SARS coronavirus (SARS-CoV) have mapped the RNA binding function to a segment of 55 residues located at the N-terminal half of the N protein and the dimerization function to its C-terminal half (14, 29, 45). In addition to its structural role, the N protein could also participate in other processes ...
... SARS coronavirus (SARS-CoV) have mapped the RNA binding function to a segment of 55 residues located at the N-terminal half of the N protein and the dimerization function to its C-terminal half (14, 29, 45). In addition to its structural role, the N protein could also participate in other processes ...
X-ray structures of the N and C-terminal domains of a
... SARS coronavirus (SARS-CoV) have mapped the RNA binding function to a segment of 55 residues located at the N-terminal half of the N protein and the dimerization function to its C-terminal half (14, 29, 45). In addition to its structural role, the N protein could also participate in other processes ...
... SARS coronavirus (SARS-CoV) have mapped the RNA binding function to a segment of 55 residues located at the N-terminal half of the N protein and the dimerization function to its C-terminal half (14, 29, 45). In addition to its structural role, the N protein could also participate in other processes ...
Proteomic Strategies to Analyze Cell
... containing human AB serum were subjected to activation for 24hrs using Staphylococcal enterotoxin B. Cell-free fractions from the activated and control cells were fractionated by twodimensional chromatography in the liquid and intact phase. To improve the sensitivity of detection of protein signatur ...
... containing human AB serum were subjected to activation for 24hrs using Staphylococcal enterotoxin B. Cell-free fractions from the activated and control cells were fractionated by twodimensional chromatography in the liquid and intact phase. To improve the sensitivity of detection of protein signatur ...
PowerPoint 演示文稿
... It is the -35 and -10 regions and the distance between them and the distance between –10 region and the transcriptionnal initiation site , that determines the transcriptional rate. The more similar the –35 and –10 regions of a promoter to the consensus sequences of TTGACA and TATAAT , the stronger a ...
... It is the -35 and -10 regions and the distance between them and the distance between –10 region and the transcriptionnal initiation site , that determines the transcriptional rate. The more similar the –35 and –10 regions of a promoter to the consensus sequences of TTGACA and TATAAT , the stronger a ...
Functional Anthology of Intrinsic Disorder. 1. Biological Processes
... experimentally confirmed disordered regions and the actual number of such proteins in nature. Although studies of functional properties of known disordered proteins are helpful in revealing the functional diversity of protein disorder, they are bound to provide only a limited view. In this study, we ...
... experimentally confirmed disordered regions and the actual number of such proteins in nature. Although studies of functional properties of known disordered proteins are helpful in revealing the functional diversity of protein disorder, they are bound to provide only a limited view. In this study, we ...
Intrinsic Disorder in Cell-signaling and Cancer
... qualitatively, we observed similar disorder prediction results for both (compare Figures 1(a) and 2(b)). Our analysis applied to these two independently constructed sets strongly supports the increased amount of disorder in proteins involved in cell-signaling and regulation. Interestingly, the prote ...
... qualitatively, we observed similar disorder prediction results for both (compare Figures 1(a) and 2(b)). Our analysis applied to these two independently constructed sets strongly supports the increased amount of disorder in proteins involved in cell-signaling and regulation. Interestingly, the prote ...
C-terminal EH-domain-containing proteins
... (Hall, 1998). Other EHD proteins, such as intersectin 1, regulate actin assembly by serving as guanine nucleotide exchange factors (GEFs) for CDC42 (Hussain et al., 2001) and binding to the Wiscott Aldrich Syndrome protein (WASp) (McGavin et al., 2001). WASp activates the Arp2/3 complex and stimulat ...
... (Hall, 1998). Other EHD proteins, such as intersectin 1, regulate actin assembly by serving as guanine nucleotide exchange factors (GEFs) for CDC42 (Hussain et al., 2001) and binding to the Wiscott Aldrich Syndrome protein (WASp) (McGavin et al., 2001). WASp activates the Arp2/3 complex and stimulat ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.