Renin Precursor Synthesis and Renin
... plasma it does not bind to other proteins. This facilitates its enzymatic activity in mouse plasma, but does not offer an explanation for the highmolecular-weight forms. In order to mimic complex-formation of renin with plasma proteins 1251-labelled pure submaxillary mol. wt. 40000 renin was mixed w ...
... plasma it does not bind to other proteins. This facilitates its enzymatic activity in mouse plasma, but does not offer an explanation for the highmolecular-weight forms. In order to mimic complex-formation of renin with plasma proteins 1251-labelled pure submaxillary mol. wt. 40000 renin was mixed w ...
Single Processing Center Models for Human Dicer and Bacterial
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
... function as a homodimer. Based on an X-ray structure of the Aquifex aeolicus RNase III, models of the enzyme interaction with dsRNA, and its cleavage at two composite catalytic centers, have been proposed. We have generated mutations in human Dicer and Escherichia coli RNase III residues implicated ...
RNA Synthesis and Degradation during Antibiotic
... this interpretation seems justified. It also seems unlikely that the lag is due to a deficiency in any of the small molecules produced by the cell, since the addition of 005% (w/v) yeast extract during the recovery period did not shorten the lag. Synthesis of DNA and protein does not begin until the ...
... this interpretation seems justified. It also seems unlikely that the lag is due to a deficiency in any of the small molecules produced by the cell, since the addition of 005% (w/v) yeast extract during the recovery period did not shorten the lag. Synthesis of DNA and protein does not begin until the ...
pdf
... purified eukaryotic polymerases. These specificity factors are present in crude nuclear extracts, because when such crude extracts were added to the purified polymerases, specific initiation at promoters was observed. Biochemists purified several transcription initiation factors by fractionating nuc ...
... purified eukaryotic polymerases. These specificity factors are present in crude nuclear extracts, because when such crude extracts were added to the purified polymerases, specific initiation at promoters was observed. Biochemists purified several transcription initiation factors by fractionating nuc ...
Characterization of a 30S Ribsomal Subunit Intermediate Found in
... ears. I am also grateful for the congregation at Friendship Baptist Church as well as Jim and Louise Kelly for their prayers and support. I would like to acknowledge the Educational Opportunity Fund program at The College of New Jersey, The Ronald McNair Program, and the BRIDGES program at the Natio ...
... ears. I am also grateful for the congregation at Friendship Baptist Church as well as Jim and Louise Kelly for their prayers and support. I would like to acknowledge the Educational Opportunity Fund program at The College of New Jersey, The Ronald McNair Program, and the BRIDGES program at the Natio ...
Systematic Characterisation of Cellular Localisation and
... DRiPs would enable MHC class I molecules to monitor protein synthesis rates rather than protein concentrations, and offer the possibility of rapid detection of virus-infected cells. One implication of the DRiP hypothesis is that the correlation between protein concentration and the probability that ...
... DRiPs would enable MHC class I molecules to monitor protein synthesis rates rather than protein concentrations, and offer the possibility of rapid detection of virus-infected cells. One implication of the DRiP hypothesis is that the correlation between protein concentration and the probability that ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
... Furthermore, ingestion of lupin-containing foods has been associated with the prevention of obesity, diabetes, and eventually cardiovascular disease. Recently, hypocholesterolaemic properties have been demonstrated for lupin conglutin γ proteins, which may decrease the risk of cardiovascular disease ...
... Furthermore, ingestion of lupin-containing foods has been associated with the prevention of obesity, diabetes, and eventually cardiovascular disease. Recently, hypocholesterolaemic properties have been demonstrated for lupin conglutin γ proteins, which may decrease the risk of cardiovascular disease ...
Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets Christian Hentrich
... encapsulation and replication into a functional model protocell presents additional challenges. Compartmentalization of genomic RNA molecules without (or with only rare) exchange between protocells is essential for any protocell model as it would allow RNA sequences with desirable properties, such a ...
... encapsulation and replication into a functional model protocell presents additional challenges. Compartmentalization of genomic RNA molecules without (or with only rare) exchange between protocells is essential for any protocell model as it would allow RNA sequences with desirable properties, such a ...
Microtubules
... as well as other proteins, are either present in centrioles or required for their formation. ...
... as well as other proteins, are either present in centrioles or required for their formation. ...
Facing extremes: archaeal surface-layer (glyco)proteins
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
A Role for A-to-I RNA Editing in Temperature Adaptation
... increases the turnover rate at negative voltages by destabilizing the deeply occluded, three Na ions bound state (11). For the human 5-HT2C serotonin receptor, a classic G-protein coupled receptor, there are five closely spaced editing sites in sequence that encode the intracellular loop that couple ...
... increases the turnover rate at negative voltages by destabilizing the deeply occluded, three Na ions bound state (11). For the human 5-HT2C serotonin receptor, a classic G-protein coupled receptor, there are five closely spaced editing sites in sequence that encode the intracellular loop that couple ...
Facing extremes: archaeal surface-layer (glyco)proteins
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
Untitled - Normalesup.org
... the whole tree of life based on nucleic acids. Even ribosomal RNA itself is ...
... the whole tree of life based on nucleic acids. Even ribosomal RNA itself is ...
AA-tRNA - Studentportalen
... initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. eIF4F is composed of 4E, 4G and 4A. 3.) The binding o ...
... initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. eIF4F is composed of 4E, 4G and 4A. 3.) The binding o ...
Global MicroRNA Amplification Kit
... B. MicroRNA and Other Small RNAs The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in th ...
... B. MicroRNA and Other Small RNAs The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in th ...
Studies on the structure and function of 16S ribosomal RNA using
... quasi-secondary conditions. We find 57 such residues that show this behaviour. (iii) An unexpectedly stable structure has been identified in the region between positions 109 and 279, where many residues remain unreactive even at 90°C in EDTAcontaining buffer. This region may correspond to a structur ...
... quasi-secondary conditions. We find 57 such residues that show this behaviour. (iii) An unexpectedly stable structure has been identified in the region between positions 109 and 279, where many residues remain unreactive even at 90°C in EDTAcontaining buffer. This region may correspond to a structur ...
Sample pages 1 PDF
... of the bacterial ribosome and its nonsymmetrical ribosomal subunits was first reconstituted from negatively stained EM images in the laboratories of Vasiliev and Lake (Lake 1976; Vasiliev 1974). Ribosomes from Bacteria and Archaea consist of a large (50S) and a small (30S) subunit, which together co ...
... of the bacterial ribosome and its nonsymmetrical ribosomal subunits was first reconstituted from negatively stained EM images in the laboratories of Vasiliev and Lake (Lake 1976; Vasiliev 1974). Ribosomes from Bacteria and Archaea consist of a large (50S) and a small (30S) subunit, which together co ...
Proteomic Approach to Identify Novel
... proteins are freshly generated by the mitochondrial processing peptidase shortly after import of proteins into the organelle. The determination of peptide masses of trypsinated spots by matrix-assisted laserdesorption ionization-mass spectrometry also led to successful identification of proteins, be ...
... proteins are freshly generated by the mitochondrial processing peptidase shortly after import of proteins into the organelle. The determination of peptide masses of trypsinated spots by matrix-assisted laserdesorption ionization-mass spectrometry also led to successful identification of proteins, be ...
- Wiley Online Library
... altered A-to-I editing of various RNAs has been associated with a wide range of diseases, such as Alzheimer’s disease or amyotrophic lateral sclerosis.60,61 In the human transcriptome, more than 99% of the editing sites are reported to be positioned in Alu sequences, which are short interspersed nuc ...
... altered A-to-I editing of various RNAs has been associated with a wide range of diseases, such as Alzheimer’s disease or amyotrophic lateral sclerosis.60,61 In the human transcriptome, more than 99% of the editing sites are reported to be positioned in Alu sequences, which are short interspersed nuc ...
RNAi: nature abhors a double-strand György Hutvágner and Phillip
... lysates [31••] is at once exciting and surprising. The fungus Neurospora crassa contains at least one RdRP homolog; the worm genome contains four such genes; Arabidopsis has six. Genetic experiments clearly demonstrate an essential role for RdRP proteins in RNAi in each of these organisms [32–34,35• ...
... lysates [31••] is at once exciting and surprising. The fungus Neurospora crassa contains at least one RdRP homolog; the worm genome contains four such genes; Arabidopsis has six. Genetic experiments clearly demonstrate an essential role for RdRP proteins in RNAi in each of these organisms [32–34,35• ...
Tracing the Archaeal Origins of Eukaryotic Membrane
... et al. 2014). While these observations suggest that this mechanism contributed significantly to early eukaryotic evolution, the deepest origins of the MTS remain unclear. This lack of insight may be due to the fact that, in contrast to proteins derived from endosymbiotic organelles (i.e., mitochondr ...
... et al. 2014). While these observations suggest that this mechanism contributed significantly to early eukaryotic evolution, the deepest origins of the MTS remain unclear. This lack of insight may be due to the fact that, in contrast to proteins derived from endosymbiotic organelles (i.e., mitochondr ...
Making protein (translation)
... Protein Synthesis Part II: Translation • Using the length of messenger RNA to assemble amino acids into proteins. • Takes place in a ribosome. ...
... Protein Synthesis Part II: Translation • Using the length of messenger RNA to assemble amino acids into proteins. • Takes place in a ribosome. ...
RNA-binding proteins
... binding domain (DS-RBD), Piwi/Argonaute/Zwille (PAZ) domain, Sm domain, etc. Many RBPs have one or more copies of the same RNA binding domain while others have two or more distinct domains. Several RNA binding domains are suggestive for the molecular function of the RBP; DEAD/DEAH box for RNA helica ...
... binding domain (DS-RBD), Piwi/Argonaute/Zwille (PAZ) domain, Sm domain, etc. Many RBPs have one or more copies of the same RNA binding domain while others have two or more distinct domains. Several RNA binding domains are suggestive for the molecular function of the RBP; DEAD/DEAH box for RNA helica ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.