Xanthomonas campestris
... were related to cellular metabolism (citrate cycle, glycolysis/gluconeogenesis, oxidative phosphorylation, other carbohydrate metabolism, and pentose phosphate pathway) (Supporting Information Fig. 2A). Moreover, a large number of proteins were associated with protein synthesis corresponding to 15% ...
... were related to cellular metabolism (citrate cycle, glycolysis/gluconeogenesis, oxidative phosphorylation, other carbohydrate metabolism, and pentose phosphate pathway) (Supporting Information Fig. 2A). Moreover, a large number of proteins were associated with protein synthesis corresponding to 15% ...
Mechanisms of mitochondrial protein import
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
... residues; they usually lack acidic amino acids. Comparison of the primary structures of these signal sequences reveals no obvious homology or motif that might be responsible for the targeting function. These signal sequences, however, can adopt an amphiphilic -helix that might be important for thei ...
Plant organelle proteomics
... subcellular locations. The tonoplast has been subject of many plant subcellular localization studies, because of the importance of the tonoplast in plant cell metabolism and the enigmatic identity of many of the membrane transport proteins. Several vacuole proteomic studies have been attempted and y ...
... subcellular locations. The tonoplast has been subject of many plant subcellular localization studies, because of the importance of the tonoplast in plant cell metabolism and the enigmatic identity of many of the membrane transport proteins. Several vacuole proteomic studies have been attempted and y ...
Chapter 21 (part 1) - University of Nevada, Reno
... • RPB1 and RPB2 are homologous to E. coli RNA polymerase b and b' • RPB1 has DNA-binding site; RPB2 binds NTP • RPB1 has C-terminal domain (CTD) or PTSPSYS • 5 of these 7 have -OH, so this is a hydrophilic and phosphorylatable site ...
... • RPB1 and RPB2 are homologous to E. coli RNA polymerase b and b' • RPB1 has DNA-binding site; RPB2 binds NTP • RPB1 has C-terminal domain (CTD) or PTSPSYS • 5 of these 7 have -OH, so this is a hydrophilic and phosphorylatable site ...
RNAzol RT (R4533) - Technical Bulletin - Sigma
... tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, washing and solubilization. Chloroform-induced phase ...
... tissue sample. The addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can be removed by centrifugation. RNA can then be isolated from the supernatant by alcohol precipitation, washing and solubilization. Chloroform-induced phase ...
Types of RNA
... can cleave complementary mRNA, block the mRNA from being translated, or accelerate its degradation. While small interfering RNAs (siRNA; 20-25 nt) are often produced by breakdown of viral RNA, there are also endogenous sources of siRNAs. siRNAs act through RNA interference in a fashion similar to mi ...
... can cleave complementary mRNA, block the mRNA from being translated, or accelerate its degradation. While small interfering RNAs (siRNA; 20-25 nt) are often produced by breakdown of viral RNA, there are also endogenous sources of siRNAs. siRNAs act through RNA interference in a fashion similar to mi ...
Document
... The three steps of transcription: initiation, elongation and termination RNA polymerase ...
... The three steps of transcription: initiation, elongation and termination RNA polymerase ...
Summer Internship project
... would be both powerful and convenient. Combined with gene expression analysis, a single RNA extraction would provide answers to a number of different questions: (i) How many microorganisms are present?; (ii) What type of microorganisms are they?; and (iii) Which metabolic pathways are they utilizing ...
... would be both powerful and convenient. Combined with gene expression analysis, a single RNA extraction would provide answers to a number of different questions: (i) How many microorganisms are present?; (ii) What type of microorganisms are they?; and (iii) Which metabolic pathways are they utilizing ...
Macromolecules Exercise Ver8 - STAR
... and was designed and created in collaboration with Shannon Donnelly (North Shore Technical High School) and David Stanley (JFYNetworks). ...
... and was designed and created in collaboration with Shannon Donnelly (North Shore Technical High School) and David Stanley (JFYNetworks). ...
Pre-mRNA splicing: life at the centre of the central dogma
... Numerous studies have demonstrated extensive coupling among different steps in eukaryotic gene expression, the best example being the intimate connection between transcription and RNA processing. More recently, new connections between nuclear and ...
... Numerous studies have demonstrated extensive coupling among different steps in eukaryotic gene expression, the best example being the intimate connection between transcription and RNA processing. More recently, new connections between nuclear and ...
Transcription termination control in bacteria Tina M Henkin
... The crystal structure of the TRAP–RNA complex revealed an elegant arrangement in which the RNA is wrapped around the TRAP wheel with each RNA triplet interacting with a binding pocket so that the 11 triplet repeats precisely match the repeat structure of the 11-mer TRAP ring [35••]. A 5′ stem-loop i ...
... The crystal structure of the TRAP–RNA complex revealed an elegant arrangement in which the RNA is wrapped around the TRAP wheel with each RNA triplet interacting with a binding pocket so that the 11 triplet repeats precisely match the repeat structure of the 11-mer TRAP ring [35••]. A 5′ stem-loop i ...
RNA polymerase II
... For example, mutations that interfere with proper splicing of β-globin mRNA are responsible for some cases of β-thalassemia. ...
... For example, mutations that interfere with proper splicing of β-globin mRNA are responsible for some cases of β-thalassemia. ...
Mini-Review Roles of Molecular Chaperones in Protein Degradation
... (16). The increased proteolysis is not simply due to effects of stress on the structure of substrate proteins; instead, molecular chaperones and proteases produced during stress are required (1, 7, 13). Bacterial proteases known to be induced by stress include two ATP-dependent proteases, La and Clp ...
... (16). The increased proteolysis is not simply due to effects of stress on the structure of substrate proteins; instead, molecular chaperones and proteases produced during stress are required (1, 7, 13). Bacterial proteases known to be induced by stress include two ATP-dependent proteases, La and Clp ...
Lab Session 9
... Why using Polyacrylamide? • It is inert • Can easily be made up at a different concentrations to produce different pore sizes. ...
... Why using Polyacrylamide? • It is inert • Can easily be made up at a different concentrations to produce different pore sizes. ...
Dr Asmat Salim MM707-electrophoresis 2014
... and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape (2) to determine molecular weight of proteins (3) to detect amino a ...
... and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape (2) to determine molecular weight of proteins (3) to detect amino a ...
NZY First-Strand cDNA Synthesis Kit
... primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. ...
... primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. ...
rna polymerases
... For example, mutations that interfere with proper splicing of β-globin mRNA are responsible for some cases of β-thalassemia. ...
... For example, mutations that interfere with proper splicing of β-globin mRNA are responsible for some cases of β-thalassemia. ...
post-transcription
... • One benefit of genes with introns is a phenomenon called alternative splicing • A pre-mRNA with multiple introns can be spliced in different ways – This will generate mature mRNAs with different combinations of exons ...
... • One benefit of genes with introns is a phenomenon called alternative splicing • A pre-mRNA with multiple introns can be spliced in different ways – This will generate mature mRNAs with different combinations of exons ...
Severa1 Proteins lmported into Chloroplasts Form
... and Murialdo, 1983; Cheng et al., 1989; Goloubinoff et al., 1989). Therefore, binding of imported proteins to cpn60, as observed in the present study, may be an early event in the folding of chloroplast proteins. It should be noted that a stable association was observed between cpn60 and the monomer ...
... and Murialdo, 1983; Cheng et al., 1989; Goloubinoff et al., 1989). Therefore, binding of imported proteins to cpn60, as observed in the present study, may be an early event in the folding of chloroplast proteins. It should be noted that a stable association was observed between cpn60 and the monomer ...
The Methylosome, a 20S Complex Containing JBP1 and pICln
... SMN oligomerization (37, 44), which greatly increases SMN⬘s affinity for SmD1, SmD3, and SmB (44). The SmD1 and SmD3 arginine- and glycine-rich (RG) carboxyl-terminal domains are necessary and sufficient for SMN binding (20). In contrast, SmB has a much longer carboxyl-terminal RG domain (approximat ...
... SMN oligomerization (37, 44), which greatly increases SMN⬘s affinity for SmD1, SmD3, and SmB (44). The SmD1 and SmD3 arginine- and glycine-rich (RG) carboxyl-terminal domains are necessary and sufficient for SMN binding (20). In contrast, SmB has a much longer carboxyl-terminal RG domain (approximat ...
RnaUs Total Viral RNA/DNA Prep
... and centrifuge at 12,000 rpm for 2 min. The RNA is in the flow-through liquid. Store the RNA solution o at -20 C. Note: It is highly recommended that RNA quality be determined before downstream applications. The quality of RNA can be assessed by denatured agarose gel electrophoresis with the ethidiu ...
... and centrifuge at 12,000 rpm for 2 min. The RNA is in the flow-through liquid. Store the RNA solution o at -20 C. Note: It is highly recommended that RNA quality be determined before downstream applications. The quality of RNA can be assessed by denatured agarose gel electrophoresis with the ethidiu ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carried out, sequencing gels revealed that cycloheximide protected two guanine residues against chemical modifica ...
... isolating the ribosomal RNA and carrying out primer extension using suitable DNA oligomers that prime the action of the enzyme reverse transcriptase. After the above experiments had been carried out, sequencing gels revealed that cycloheximide protected two guanine residues against chemical modifica ...
REVIEWS How membrane proteins travel across the mitochondrial
... guide the precursor across (both in yellow) of the intermembrane space (IMS). The the aqueous intermembrane bound precursor is usually delivered to an insertion space, yielding a soluble incomplex (yellow) composed of Tim9p, Tim10p, Tim12p, Tim22p and Tim54p that catalyzes the membrane termediate in ...
... guide the precursor across (both in yellow) of the intermembrane space (IMS). The the aqueous intermembrane bound precursor is usually delivered to an insertion space, yielding a soluble incomplex (yellow) composed of Tim9p, Tim10p, Tim12p, Tim22p and Tim54p that catalyzes the membrane termediate in ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.