Small-angle scattering studies of intrinsically disordered proteins
... Introduction In the last two decades, Intrinsically Disordered Proteins or Regions (IDPs/IDRs) have emerged as fundamental molecules in a broad range of crucial biological functions such as cell signaling, regulation, and homeostasis [1,2,3**]. Due to their lack of a permanent secondary and tertiar ...
... Introduction In the last two decades, Intrinsically Disordered Proteins or Regions (IDPs/IDRs) have emerged as fundamental molecules in a broad range of crucial biological functions such as cell signaling, regulation, and homeostasis [1,2,3**]. Due to their lack of a permanent secondary and tertiar ...
Protein Targeting into the Complex Plastid of Cryptophytes
... To generate a set of nucleus-encoded plastid proteins (NuPPs) we first focused on the identification of conserved genes encoding proteins and enzymes being unique and essential for photosynthesis. Identification of nucleus-encoded proteins located in the PPC proved to be more complicated, because se ...
... To generate a set of nucleus-encoded plastid proteins (NuPPs) we first focused on the identification of conserved genes encoding proteins and enzymes being unique and essential for photosynthesis. Identification of nucleus-encoded proteins located in the PPC proved to be more complicated, because se ...
The RNA-binding protein repertoire of embryonic
... RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-link ...
... RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-link ...
Glutathionylation in the Photosynthetic Model Organism
... respectively. Biotinylated glutathione ethyl ester (BioGEE) was obtained from Invitrogen. High performance liquid chromatography grade ethanol and acetic acid were purchased from VWR France, and all of the other reagents were from Sigma-Aldrich. Chlamydomonas Cultures and Protein Extraction—The C. r ...
... respectively. Biotinylated glutathione ethyl ester (BioGEE) was obtained from Invitrogen. High performance liquid chromatography grade ethanol and acetic acid were purchased from VWR France, and all of the other reagents were from Sigma-Aldrich. Chlamydomonas Cultures and Protein Extraction—The C. r ...
Molecular Chaperones in the Cytosol: from Nascent Chain to Folded
... folding mainly through two distinct mechanisms. Chaperones, such as trigger factor and the Hsp70s, act by holding nascent and newly synthesized chains in a state competent for folding upon release into the medium. In contrast, the large, cylindrical chaperonin complexes provide physically defined co ...
... folding mainly through two distinct mechanisms. Chaperones, such as trigger factor and the Hsp70s, act by holding nascent and newly synthesized chains in a state competent for folding upon release into the medium. In contrast, the large, cylindrical chaperonin complexes provide physically defined co ...
PowerPoint 簡報
... • Rho is an RNA helicase, composed of 6 identical subunits, each subunit has an RNA binding domain and ATPase domain, requires ATP to function. • Rho releases the RNA product from the DNA template. ...
... • Rho is an RNA helicase, composed of 6 identical subunits, each subunit has an RNA binding domain and ATPase domain, requires ATP to function. • Rho releases the RNA product from the DNA template. ...
Proteomic analyses of the time course responses of mice infected
... (Silva et al., 2010). For data analysis, spot matching across all gels without omitting values was required for spot merging. Each gel was processed in three replicates in parallel with three independent protein preparations. A single average gel was generated from three independent subgels containi ...
... (Silva et al., 2010). For data analysis, spot matching across all gels without omitting values was required for spot merging. Each gel was processed in three replicates in parallel with three independent protein preparations. A single average gel was generated from three independent subgels containi ...
Phosphoproteomics reveals extensive in vivo phosphorylation of
... purified by IMAC by two different protocols (Materials and Methods). Esterification of peptides before IMAC has been shown to greatly improve the abundance of phosphopeptides in the isolated sample (19). The protocol that included the esterification step indeed gave the highest purity of phosphopept ...
... purified by IMAC by two different protocols (Materials and Methods). Esterification of peptides before IMAC has been shown to greatly improve the abundance of phosphopeptides in the isolated sample (19). The protocol that included the esterification step indeed gave the highest purity of phosphopept ...
high-performance gene expression
... quadruplex reactions (red line). The results illustrate that SensiFAST Probe No-ROX has high sensitivity and excellent reproducibility for both singleplex and multiplex reactions (Fig. 7A) and no reduction of efficiency (Fig. 7B) that is often associated with multiplexing. There is no change in Ct v ...
... quadruplex reactions (red line). The results illustrate that SensiFAST Probe No-ROX has high sensitivity and excellent reproducibility for both singleplex and multiplex reactions (Fig. 7A) and no reduction of efficiency (Fig. 7B) that is often associated with multiplexing. There is no change in Ct v ...
Architecture of the trypanosome RNA editing accessory complex
... governing the sites and numbers of uridines to be inserted and deleted is provided by the mostly (with two exceptions) minicircle-encoded guide RNAs (gRNAs). RNA editing is catalyzed by the multiprotein RNA editing core complex (RECC), also known as the editosome. Pre-mRNA and cognate gRNA form an a ...
... governing the sites and numbers of uridines to be inserted and deleted is provided by the mostly (with two exceptions) minicircle-encoded guide RNAs (gRNAs). RNA editing is catalyzed by the multiprotein RNA editing core complex (RECC), also known as the editosome. Pre-mRNA and cognate gRNA form an a ...
7.12. PROTEIN FOLDING AND MISFOLDING43
... and plaques in tissues include Alzheimer’s and Parkinson’s diseases, spongiform encephalopathy (mad cow disease) and some forms of diabetes to name just a few. Each amyloid disease involves aggregation of a specific protein. Although native structures of known proteins involved in characterized amyl ...
... and plaques in tissues include Alzheimer’s and Parkinson’s diseases, spongiform encephalopathy (mad cow disease) and some forms of diabetes to name just a few. Each amyloid disease involves aggregation of a specific protein. Although native structures of known proteins involved in characterized amyl ...
TRANSPORT PROCESSES
... signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are usually not present in the “mature” proteins found in cells. The hydrophobic residues in the core of ER signal sequences form a binding site that is critical for the interaction of signa ...
... signal sequence is cleaved from the protein while it is still growing on the ribosome; thus, signal sequences are usually not present in the “mature” proteins found in cells. The hydrophobic residues in the core of ER signal sequences form a binding site that is critical for the interaction of signa ...
RNA-Seq Sample Recommendations (Craig Praul, PSU and Caitlyn
... concentration as this device is easy to use and less prone to error than traditional spectrophotometers because sample dilution is usually unnecessary and pipetting errors have no effect on correct determination of concentration. All facility customers may use the one provided at the PSU genome core ...
... concentration as this device is easy to use and less prone to error than traditional spectrophotometers because sample dilution is usually unnecessary and pipetting errors have no effect on correct determination of concentration. All facility customers may use the one provided at the PSU genome core ...
(2) rRNA
... (4) During translation, the ribosome moves three nucleotides at a time along the mRNA in the 5’- to 3’- direction, allowing aatRNAs to pair with larger mRNA through codon-anticodon pairing (Fig. 2.25, 2.26). i. Actually, only two bases is sufficient to direct the codonanticodon interaction. In other ...
... (4) During translation, the ribosome moves three nucleotides at a time along the mRNA in the 5’- to 3’- direction, allowing aatRNAs to pair with larger mRNA through codon-anticodon pairing (Fig. 2.25, 2.26). i. Actually, only two bases is sufficient to direct the codonanticodon interaction. In other ...
Abstract 1
... It appears that multiple binding does not occur until all available RNA molecules have been complexed with a single neomycin. If a second, stochiometric quantity of neomycin is added at this point, a second binding site on the RNA molecules appears to be occupied. A similar observation is made with ...
... It appears that multiple binding does not occur until all available RNA molecules have been complexed with a single neomycin. If a second, stochiometric quantity of neomycin is added at this point, a second binding site on the RNA molecules appears to be occupied. A similar observation is made with ...
Assembly and function of cell surface structures of the
... Ligated RNA-reverse transcriptase PCR (LRRT-PCR, Mandl et al., 1991) was performed with total A. ambivalens RNA in order to confirm the transcription start site independently, to map the transcriptional terminators, to demonstrate that the internal transcription terminator is active, and to show tha ...
... Ligated RNA-reverse transcriptase PCR (LRRT-PCR, Mandl et al., 1991) was performed with total A. ambivalens RNA in order to confirm the transcription start site independently, to map the transcriptional terminators, to demonstrate that the internal transcription terminator is active, and to show tha ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... Because of this ancestral relationship, orthologs represent the evolutionary history of species most accurately and also may perform similar function in different domains of lives such as halophiles, thermophiles and mesophiles. While proteins from mesophiles work under normal environmental conditio ...
... Because of this ancestral relationship, orthologs represent the evolutionary history of species most accurately and also may perform similar function in different domains of lives such as halophiles, thermophiles and mesophiles. While proteins from mesophiles work under normal environmental conditio ...
Domain organization of human cleavage factor Im 1 Distinct
... subunits. Binding of CF!Im to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. We identified regions in the subunits of CF!Im involved in RNAbinding, protein-protein interactions, and ...
... subunits. Binding of CF!Im to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. We identified regions in the subunits of CF!Im involved in RNAbinding, protein-protein interactions, and ...
The archaeal origins of the eukaryotic translational system
... both the full 67- and the 33-protein subset of only those common to Archaea and Eukarya provided a clear three-way separation between Eukarya, Crenarchaea and Euryarchaea (Figure 4b). The same relationship was obtained using only the blocks common to both Eukarya and Archaea found in the 34 universa ...
... both the full 67- and the 33-protein subset of only those common to Archaea and Eukarya provided a clear three-way separation between Eukarya, Crenarchaea and Euryarchaea (Figure 4b). The same relationship was obtained using only the blocks common to both Eukarya and Archaea found in the 34 universa ...
RNA/DNA catalysts
... Know four types of natural catalytic RNAs (group I introns, group II introns, RNase P, small self-cleaving), what reactions they perform, know basics of their secondary and tertiary structure, requirements for cofactors/metals/proteins/ATP Know details of glmS ribozyme self-cleavage Understand use o ...
... Know four types of natural catalytic RNAs (group I introns, group II introns, RNase P, small self-cleaving), what reactions they perform, know basics of their secondary and tertiary structure, requirements for cofactors/metals/proteins/ATP Know details of glmS ribozyme self-cleavage Understand use o ...
Bioinformatics analysis of experimentally determined protein
... once a more complete list of all essential complexes becomes available. The Supplementary Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, wh ...
... once a more complete list of all essential complexes becomes available. The Supplementary Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, wh ...
Do Legume Storage Proteins Play a Role in
... aggregate to form hexamers and are also known as 11S storage globulins (Shewry et al., 1995). Vicilins, also known as 7S storage globulins, are usually single-chain proteins without disulfide bonds. They aggregate to form trimers of subunits with varying molecular masses (45–53 kD). They are also en ...
... aggregate to form hexamers and are also known as 11S storage globulins (Shewry et al., 1995). Vicilins, also known as 7S storage globulins, are usually single-chain proteins without disulfide bonds. They aggregate to form trimers of subunits with varying molecular masses (45–53 kD). They are also en ...
RNA Biology: Structures to the people! | eLife
... he structures of molecules often hold the key to understanding their roles in cells. Thus, when Watson and Crick proposed the double-helix structure for DNA, they immediately speculated on how DNA may replicate. Unfortunately, working out the structures of RNA molecules is challenging, and the techn ...
... he structures of molecules often hold the key to understanding their roles in cells. Thus, when Watson and Crick proposed the double-helix structure for DNA, they immediately speculated on how DNA may replicate. Unfortunately, working out the structures of RNA molecules is challenging, and the techn ...
Molecular Biology
... (A) The promoter contains a DNA sequence called the TATA box, which is located 25 nucleotides away from the site where transcription is initiated. (B) The TATA box is recognized and bound by transcription factor TFIID, which then enables the adjacent binding of TFIIB (C). (D) The rest of the general ...
... (A) The promoter contains a DNA sequence called the TATA box, which is located 25 nucleotides away from the site where transcription is initiated. (B) The TATA box is recognized and bound by transcription factor TFIID, which then enables the adjacent binding of TFIIB (C). (D) The rest of the general ...
BIOLOGY
... combinations of structural elements formed by the folding of nearby amino acid sequences. An example of a motif is a helix-loop-helix, which binds to DNA. Very similar motifs are found in many proteins that are not related. Scientists have classified conserved domains and motifs in a number of datab ...
... combinations of structural elements formed by the folding of nearby amino acid sequences. An example of a motif is a helix-loop-helix, which binds to DNA. Very similar motifs are found in many proteins that are not related. Scientists have classified conserved domains and motifs in a number of datab ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.