EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c
... structures at undetectable levels. For experiments involving induced antibodies, such as these, TLC is used in our laboratory only qualitatively to differentiate acidic and amide residues as the results are difficult to quantitate. All positions in these sequencer runs were initially analyzed by GC ...
... structures at undetectable levels. For experiments involving induced antibodies, such as these, TLC is used in our laboratory only qualitatively to differentiate acidic and amide residues as the results are difficult to quantitate. All positions in these sequencer runs were initially analyzed by GC ...
Supplementary Figure 1
... evolution; therefore the branch lengths correspond to amino acid substitutions per time unit. Evidently, UbS27a domains are less conserved than ribosomal S27a domains or homologs to SUMO1, despite the presence of a hypervariable loop at the N-terminus of SUMO1. Particularly indicative of a high rate ...
... evolution; therefore the branch lengths correspond to amino acid substitutions per time unit. Evidently, UbS27a domains are less conserved than ribosomal S27a domains or homologs to SUMO1, despite the presence of a hypervariable loop at the N-terminus of SUMO1. Particularly indicative of a high rate ...
Protein Data Condensation for Effective Quaternary Structure
... The techniques presented in [10, 16, 22] are able to distinguish just between two classes, that are, homodimers and non-homodimers, whereas our approach is able to discriminate among any number of classes. In this respect, our method is more similar to the two approaches illustrated in the followin ...
... The techniques presented in [10, 16, 22] are able to distinguish just between two classes, that are, homodimers and non-homodimers, whereas our approach is able to discriminate among any number of classes. In this respect, our method is more similar to the two approaches illustrated in the followin ...
mTORC1 regulates the efficiency and cellular capacity for protein
... to a site on eIF4E similar to that occupied by eIF4G, thus sequestering eIF4E. 4E-BPs are phosphorylated by mTORC1, leading to the release of eIF4E which can now bind eIF4G (Figure 1). Since mTORC1 can regulate the recruitment of eIF4A and the activity of eIF4B, it has been thought that mTORC1 signa ...
... to a site on eIF4E similar to that occupied by eIF4G, thus sequestering eIF4E. 4E-BPs are phosphorylated by mTORC1, leading to the release of eIF4E which can now bind eIF4G (Figure 1). Since mTORC1 can regulate the recruitment of eIF4A and the activity of eIF4B, it has been thought that mTORC1 signa ...
Introns and mutations
... G cap Poly-A tail (hnRNA) Transcripts are subject to a third modification. If you looked at the sequence of a transcript, you would not find anything in one region to distinguish it from another. Me ...
... G cap Poly-A tail (hnRNA) Transcripts are subject to a third modification. If you looked at the sequence of a transcript, you would not find anything in one region to distinguish it from another. Me ...
Electromagnetic Properties of Biomolecules
... conclusion was drawn: each specific biological function within the protein or DNA is characterised by one frequency. It has been shown in previous research that all protein sequences with the common biological function have common frequency component, which is a specific feature for the observed fu ...
... conclusion was drawn: each specific biological function within the protein or DNA is characterised by one frequency. It has been shown in previous research that all protein sequences with the common biological function have common frequency component, which is a specific feature for the observed fu ...
Regulation of transcription by Saccharomyces cerevisiae 14-3
... signalling. The inactive form of Rtg3 is sequestered in the cytoplasm [23]. The expression of six genes involved in glyoxylate, the first steps of gluconeogenesis and glutamate metabolism is known to be regulated by Rtg3 [37]. As shown in Table 5, the steady-state mRNA levels of these six genes are ...
... signalling. The inactive form of Rtg3 is sequestered in the cytoplasm [23]. The expression of six genes involved in glyoxylate, the first steps of gluconeogenesis and glutamate metabolism is known to be regulated by Rtg3 [37]. As shown in Table 5, the steady-state mRNA levels of these six genes are ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... the central domain, with no currently defined function [9]. Two different ACC forms exist, a homomeric form found in most eukaryotes and a heteromeric form found in most prokaryotes. The homomeric form exists as one large polypeptide where all the subunits are on one protein with sizes typically in ...
... the central domain, with no currently defined function [9]. Two different ACC forms exist, a homomeric form found in most eukaryotes and a heteromeric form found in most prokaryotes. The homomeric form exists as one large polypeptide where all the subunits are on one protein with sizes typically in ...
Detailed proteome analysis of growing cells of the planctomycete
... them were not previously identified in our master gel. Some spots that are a mixture of different proteins could be separated in this zoom-in pH range, allowing us to gain quantitative information about these proteins (Fig. 2). For example, the protein spot containing GroES and RpsF at the 10 kDa mo ...
... them were not previously identified in our master gel. Some spots that are a mixture of different proteins could be separated in this zoom-in pH range, allowing us to gain quantitative information about these proteins (Fig. 2). For example, the protein spot containing GroES and RpsF at the 10 kDa mo ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... oocytes expressing pGpLI-R18 were incubated with IgGSepharose (Fig. 1B). This resulted in a cellular extract depleted of 14-3-3 proteins, and an eluate containing both pGpLI-R18 and 14-3-3 proteins. This indicates that a large portion of cellular 14-3-3 proteins are bound to the 14-3-3 scavenger: co ...
... oocytes expressing pGpLI-R18 were incubated with IgGSepharose (Fig. 1B). This resulted in a cellular extract depleted of 14-3-3 proteins, and an eluate containing both pGpLI-R18 and 14-3-3 proteins. This indicates that a large portion of cellular 14-3-3 proteins are bound to the 14-3-3 scavenger: co ...
GLP 019 - University of Newcastle
... TRIZOL Reagent (U.S.Patent No.5,346,994) is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method developed by Chomczynski and Sacchi (ref). D ...
... TRIZOL Reagent (U.S.Patent No.5,346,994) is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method developed by Chomczynski and Sacchi (ref). D ...
domaination
... Protein domain identification and improved sequence searching using PSI-BLAST (George & Heringa, Prot. Struct. Func. Genet., in press; 2002) ...
... Protein domain identification and improved sequence searching using PSI-BLAST (George & Heringa, Prot. Struct. Func. Genet., in press; 2002) ...
Protein Detection Methods in Proteomics Research
... Blue G-250 powder is added per 1.5 l solution. The gel is stained for 4–5 days on an orbital shaker whereby the end-point is reached after 4–5 days. Subsequently, the gel is washed a few times in water to remove background staining. On 1.5 mm gels a detection limit of about 15 ng per spot is reached ...
... Blue G-250 powder is added per 1.5 l solution. The gel is stained for 4–5 days on an orbital shaker whereby the end-point is reached after 4–5 days. Subsequently, the gel is washed a few times in water to remove background staining. On 1.5 mm gels a detection limit of about 15 ng per spot is reached ...
human-physiology-ii-lecture-endomembrane
... * One that interacts with the ribosome to block further translation; * One that binds to the ER membrane (docking The site to protein:receptor protein) recognize and bind to ER ...
... * One that interacts with the ribosome to block further translation; * One that binds to the ER membrane (docking The site to protein:receptor protein) recognize and bind to ER ...
Protein Synthesis and the Stress Response
... the efficiency of translation in this condition, which would be necessary to cope with the degradation of proteins due to loss of function and/or structure [55]. There are some reports in the literature that show that an increase in error rates during protein synthesis enhances protein oxidation. In ...
... the efficiency of translation in this condition, which would be necessary to cope with the degradation of proteins due to loss of function and/or structure [55]. There are some reports in the literature that show that an increase in error rates during protein synthesis enhances protein oxidation. In ...
BMC Genomics Functional genomics of HMGN3a and SMARCAL1 in early mammalian embryogenesis
... Sucrose Non-Fermenting in yeast) ATP-dependent chromatin remodeling complex [12]. SWI/SNF complexes are thought to regulate transcription of certain genes by altering the chromatin structure around them with their helicase and ATPase activities [13,14]. In mammals, each SWI/SNF complex has any of tw ...
... Sucrose Non-Fermenting in yeast) ATP-dependent chromatin remodeling complex [12]. SWI/SNF complexes are thought to regulate transcription of certain genes by altering the chromatin structure around them with their helicase and ATPase activities [13,14]. In mammals, each SWI/SNF complex has any of tw ...
Email:
... Studied mechanism of Cisplatin-RNA interaction by reverse transcription, HPLC, MALDI-TOF, LC-MS and Atomic absorption spectroscopy. For advanced understanding of how small molecules find their target sites; various analogues of cisplatin were synthesized, characterized, and studied their interac ...
... Studied mechanism of Cisplatin-RNA interaction by reverse transcription, HPLC, MALDI-TOF, LC-MS and Atomic absorption spectroscopy. For advanced understanding of how small molecules find their target sites; various analogues of cisplatin were synthesized, characterized, and studied their interac ...
Nonsense-mediated mRNA decay - Case Western Reserve University
... of the 50 splice site following splicing [36–40]. Furthermore, an interaction between the EJC protein Y14 and Upf3 is required to trigger mRNA decay, thus providing a physical and functional link between the EJC and NMD [41!!]. An mRNA can also be recognized as aberrant through mRNP features indepen ...
... of the 50 splice site following splicing [36–40]. Furthermore, an interaction between the EJC protein Y14 and Upf3 is required to trigger mRNA decay, thus providing a physical and functional link between the EJC and NMD [41!!]. An mRNA can also be recognized as aberrant through mRNP features indepen ...
Structural bioinformatics Amino acids – the building blocks of proteins
... Metal ions may stabilize the protein structure (e.g. in zinc fingers) ...
... Metal ions may stabilize the protein structure (e.g. in zinc fingers) ...
ARF1 and SAR1 GTPases in Endomembrane Trafficking in Plants
... mammalian system. It was also established in the same system that COPI proteins are involved in transport along the endocytic pathway [19,20]. During the selective transport of vesicles, the coat proteins must distinguish between cargo and resident proteins of the donor organelle. In intracellular t ...
... mammalian system. It was also established in the same system that COPI proteins are involved in transport along the endocytic pathway [19,20]. During the selective transport of vesicles, the coat proteins must distinguish between cargo and resident proteins of the donor organelle. In intracellular t ...
21 О О О Termination of Polypeptide Synthesis Requires a Stop
... elongation begin on free cytosolic ribosomes. Proteins of the secretory pathway have a hydrophobic signal peptide, usually at or near their amino terminus. There is no unique signal peptide sequence, but its characteristics include a positively charged N terminus, a core of 8–12 hydrophobic amino ac ...
... elongation begin on free cytosolic ribosomes. Proteins of the secretory pathway have a hydrophobic signal peptide, usually at or near their amino terminus. There is no unique signal peptide sequence, but its characteristics include a positively charged N terminus, a core of 8–12 hydrophobic amino ac ...
Protein Denaturation Studies Using the Pyris 6 DSC
... devices. The advantage of the Pyris 6 DSC over microcalorimeters, however, is that the DSC 6 permits the analyses of solids or frozen solutions as well as liquids. This is useful for studying the freeze-drying properties of aqueous formulations. The Pyris 6 DSC provides ease of use, flexibility, wid ...
... devices. The advantage of the Pyris 6 DSC over microcalorimeters, however, is that the DSC 6 permits the analyses of solids or frozen solutions as well as liquids. This is useful for studying the freeze-drying properties of aqueous formulations. The Pyris 6 DSC provides ease of use, flexibility, wid ...
Materials and Methods
... buried Gly, buried Phe, buried Thr, Cys, and Ile content primarily opposed permissiveness. Fractional exposed Asp content was primarily enhancing, while net charge and fractional Arg content primarily opposed enhancement. All other significant predictors had a similar influence on permissiveness vs. ...
... buried Gly, buried Phe, buried Thr, Cys, and Ile content primarily opposed permissiveness. Fractional exposed Asp content was primarily enhancing, while net charge and fractional Arg content primarily opposed enhancement. All other significant predictors had a similar influence on permissiveness vs. ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.