PSI - European Bioinformatics Institute
... • Given a set of uncharacterised sequences, we usually want to know: ...
... • Given a set of uncharacterised sequences, we usually want to know: ...
F factor conjugation is a true type IV secretion system
... C-terminal domain of TraG, TraN and TraU. F-pilin subunits are stored as a pool in the inner membrane prior to assembly on the cell surface [43]. Pili are assembled by addition of pilin subunits to the base of the pilus, as demonstrated by H-pili of R27 [44]. In response to contact with a suitable r ...
... C-terminal domain of TraG, TraN and TraU. F-pilin subunits are stored as a pool in the inner membrane prior to assembly on the cell surface [43]. Pili are assembled by addition of pilin subunits to the base of the pilus, as demonstrated by H-pili of R27 [44]. In response to contact with a suitable r ...
Nup153 is an M9containing mobile nucleoporin with a novel
... region is an M9 NLS. We found that both import and export receptors interact with several regions of Nup153, in a RanGTP-regulated fashion. RanGTP dissociates Nup153–import receptor complexes, but is required for Nup153–export receptor interactions. We also show that Nup153 is a RanGDP-binding prote ...
... region is an M9 NLS. We found that both import and export receptors interact with several regions of Nup153, in a RanGTP-regulated fashion. RanGTP dissociates Nup153–import receptor complexes, but is required for Nup153–export receptor interactions. We also show that Nup153 is a RanGDP-binding prote ...
Engineering and Identifying Supercharged Proteins
... able to adopt large, stable three-dimensional conformations suitable for strong binding to targets even when they lack hydrophobic clefts commonly associated with small-molecule binding. Moreover, the strength of macromolecule-target binding can be sufficient to interfere with native protein–protein ...
... able to adopt large, stable three-dimensional conformations suitable for strong binding to targets even when they lack hydrophobic clefts commonly associated with small-molecule binding. Moreover, the strength of macromolecule-target binding can be sufficient to interfere with native protein–protein ...
+TIPs and Microtubule Regulation. The Beginning of the Plus End in
... like treadmilling proteins, appear to surf the plus end but are not transported through the cytoplasm with the growing MT (Carvalho et al., 2003). Some hitchhiking proteins participate in MT capture by serving as bridging proteins between MTs and receptor proteins at a capture site (Gundersen et al. ...
... like treadmilling proteins, appear to surf the plus end but are not transported through the cytoplasm with the growing MT (Carvalho et al., 2003). Some hitchhiking proteins participate in MT capture by serving as bridging proteins between MTs and receptor proteins at a capture site (Gundersen et al. ...
Inquiry into Life Twelfth Edition
... • Binds to the minor groove of the TATA box – Saddle-shaped TBP lines up with DNA – Underside of the saddle forces open the minor ...
... • Binds to the minor groove of the TATA box – Saddle-shaped TBP lines up with DNA – Underside of the saddle forces open the minor ...
molecular biology
... with ‘start’ and ‘stop’ signals. The addition of monomers occurs on the template. These monomers come one after another based on the sequence information coded in the template and attach to the template molecule. A covalent bond like phosphodiester bond joins the adjacent nucleotides in DNA and RNA ...
... with ‘start’ and ‘stop’ signals. The addition of monomers occurs on the template. These monomers come one after another based on the sequence information coded in the template and attach to the template molecule. A covalent bond like phosphodiester bond joins the adjacent nucleotides in DNA and RNA ...
Phytopathogen type III effector weaponry and their plant targets
... by HopU1 possibly decreases their ability to bind, stabilize or process RNA [41]. It is not known how this alteration of RNA-binding leads to the suppression of innate immunity or if it alters specific or general RNA metabolism. Nevertheless, the post-transcriptional control of RNA by T3Es represe ...
... by HopU1 possibly decreases their ability to bind, stabilize or process RNA [41]. It is not known how this alteration of RNA-binding leads to the suppression of innate immunity or if it alters specific or general RNA metabolism. Nevertheless, the post-transcriptional control of RNA by T3Es represe ...
Chapter 1: Bio Primer - Columbia CS
... Proteins Interact Via Active Sites Protein interactions are defined by active sites E.g., antibody with pathogen E.g., drug design ...
... Proteins Interact Via Active Sites Protein interactions are defined by active sites E.g., antibody with pathogen E.g., drug design ...
Plant Cell Vacuoles
... results in secretion. Addition of the propeptides to different secreted proteins results in their vacuolar localization. These propeptides thus contain vacuolar sorting signals (VSS). Other vacuolar proteins appear to contain internal VSS. Comparison of sequences revealed that N-terminal propeptides ...
... results in secretion. Addition of the propeptides to different secreted proteins results in their vacuolar localization. These propeptides thus contain vacuolar sorting signals (VSS). Other vacuolar proteins appear to contain internal VSS. Comparison of sequences revealed that N-terminal propeptides ...
Targeting of interleukin-2 to the periplasm of
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
... Inclusion bodies located at the pole caps of cells were detected using immunogoldlabelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was obse ...
Central Dogma of Molecular Biology Chapter 28 DNA Replication
... At least 15% of all genetic diseases have been associated with mutations that affect RNA splicing. Moreover, the same pre-mRNA can be spliced differently in various cell types, at different stages of development, or in response to other biological signals. (Alternative Splicing) In addition, individ ...
... At least 15% of all genetic diseases have been associated with mutations that affect RNA splicing. Moreover, the same pre-mRNA can be spliced differently in various cell types, at different stages of development, or in response to other biological signals. (Alternative Splicing) In addition, individ ...
A biofilm-forming marine bacterium producing proteins
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
PROTEIN SYNTHESIS
... The protein complex eIF4F consists of the cap binding protein eIF4E, the ATP-dependent helicase eIF4A, and the adapter protein eIF4G upon which the complex assembles. Also associated with eIF4F is a protein kinase, Mnk1, that phosphorylates eIF4E, activating initiation of translation. The large init ...
... The protein complex eIF4F consists of the cap binding protein eIF4E, the ATP-dependent helicase eIF4A, and the adapter protein eIF4G upon which the complex assembles. Also associated with eIF4F is a protein kinase, Mnk1, that phosphorylates eIF4E, activating initiation of translation. The large init ...
Intrinsically Disordered Proteins (IDPs)
... Why are IDPs / IDRs unstructured? • To a first approximation, amino acid composition determines whether a protein folds or remains intrinsically disordered. • Given a composition that favors folding, the sequence details determine which fold. ...
... Why are IDPs / IDRs unstructured? • To a first approximation, amino acid composition determines whether a protein folds or remains intrinsically disordered. • Given a composition that favors folding, the sequence details determine which fold. ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... This result indicates strongly that ribosomes from the drug-sensitive S-cerevisiae strain have a.target site for cycloheximide that is located on 255 ribosomal RNA. By locating the position of the residues in published secondary structure models for yeast RNA it is clear that the protected residues ...
... This result indicates strongly that ribosomes from the drug-sensitive S-cerevisiae strain have a.target site for cycloheximide that is located on 255 ribosomal RNA. By locating the position of the residues in published secondary structure models for yeast RNA it is clear that the protected residues ...
The Tobacco Homolog of Mammalian Calreticulin
... or may be part of the structure of reticuloplasmin retention signals (Haugejorden et al., 1991; Denecke et al., 1992) and that retention cannot be predicted based on the primary sequence. This notion is illustrated by the fact that the auxin binding protein ABPl contains the KDEL motif but does not ...
... or may be part of the structure of reticuloplasmin retention signals (Haugejorden et al., 1991; Denecke et al., 1992) and that retention cannot be predicted based on the primary sequence. This notion is illustrated by the fact that the auxin binding protein ABPl contains the KDEL motif but does not ...
Oxidized Amino Acids in Lens Protein with Age
... were obtained from the South Carolina Lions Eye Bank, Columbia, SC and the Medical College of Georgia Eye Bank, Augusta, GA. Individual whole lenses wereweighed and then decapsulated. The lens was placed in 1 ml of ice-cold deionized water; after 10-15 min, the sample was vortexed gently, and the ca ...
... were obtained from the South Carolina Lions Eye Bank, Columbia, SC and the Medical College of Georgia Eye Bank, Augusta, GA. Individual whole lenses wereweighed and then decapsulated. The lens was placed in 1 ml of ice-cold deionized water; after 10-15 min, the sample was vortexed gently, and the ca ...
h-Barrel membrane protein folding and structure viewed through the
... protomer has non-a, non-h structure with one turn of a 310 helix and forms both polar and nonpolar contacts with the inner h sheet of its neighboring protomer [17]. It is possible that the amino latches of aHL and LukF have different conformations in their respective oligomers. The stem domain, whic ...
... protomer has non-a, non-h structure with one turn of a 310 helix and forms both polar and nonpolar contacts with the inner h sheet of its neighboring protomer [17]. It is possible that the amino latches of aHL and LukF have different conformations in their respective oligomers. The stem domain, whic ...
Stress puts TIA on TOP
... 59TOP mRNAs are regulated in a tissue- and cell typespecific manner. For example, eEF2 mRNA confers growthdependent regulation in cells of hematopoietic, but not in cells of nonhematopoietic, origins, while translation of b1-tubulin mRNA, which possesses all classical features of TOP mRNAs, is absol ...
... 59TOP mRNAs are regulated in a tissue- and cell typespecific manner. For example, eEF2 mRNA confers growthdependent regulation in cells of hematopoietic, but not in cells of nonhematopoietic, origins, while translation of b1-tubulin mRNA, which possesses all classical features of TOP mRNAs, is absol ...
Amino_Acids_and_Protein_Background_Info_
... fish, and red meats in various combinations, can also provide the complete proteins that contain the amino acids essential to the body's health and well-being. (See PROTEIN IN FOODS in Resources.) Denatured Proteins When proteins, for any reason, lose their natural folded shape, they are called dena ...
... fish, and red meats in various combinations, can also provide the complete proteins that contain the amino acids essential to the body's health and well-being. (See PROTEIN IN FOODS in Resources.) Denatured Proteins When proteins, for any reason, lose their natural folded shape, they are called dena ...
University of Groningen Sugar transport in
... anchoring region. Archaeal binding proteins studied so far are glycosylated, and it seems plausible that the flexible linker region is the site of glycosylation as demonstrated for the S-layer protein of Haloferax volcanii [23]. The P. furiosus binding proteins contain glucose moieties [17] (unpubli ...
... anchoring region. Archaeal binding proteins studied so far are glycosylated, and it seems plausible that the flexible linker region is the site of glycosylation as demonstrated for the S-layer protein of Haloferax volcanii [23]. The P. furiosus binding proteins contain glucose moieties [17] (unpubli ...
Protein Misfolding and Disease Protein Misfolding and Disease
... prone to transition from the functional conformation to a conformation dominated by β-sheets, which may aggregate in the respective compartment where the particular protein is located or be excluded to form extracellular amyloid bodies. It is believed that intrinsic instability owing to a low transi ...
... prone to transition from the functional conformation to a conformation dominated by β-sheets, which may aggregate in the respective compartment where the particular protein is located or be excluded to form extracellular amyloid bodies. It is believed that intrinsic instability owing to a low transi ...
Ubiquitin-Mediated Control of Plant Hormone
... UPS activity involves a three-step enzymatic cascade between E1, E2, and E3 enzymes that results in the covalent transfer of ubiquitin to target proteins. This process can result in different outcomes, including (1) proteolytic degradation by the 26S proteasome or reversible, nonproteolytic regulato ...
... UPS activity involves a three-step enzymatic cascade between E1, E2, and E3 enzymes that results in the covalent transfer of ubiquitin to target proteins. This process can result in different outcomes, including (1) proteolytic degradation by the 26S proteasome or reversible, nonproteolytic regulato ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.