Full Text - the American Society of Animal Science
... positive correlation coefficients were found between the FABP-4 protein level and adipocyte number (R2 = 0.47, P = 0.02) and lipid content (R2 = 0.58, P = 0.004). Conversely, there was no difference between groups relative to FABP-3 mRNA (P = 0.46) or protein (P = 0.56) levels, oxidative enzymatic a ...
... positive correlation coefficients were found between the FABP-4 protein level and adipocyte number (R2 = 0.47, P = 0.02) and lipid content (R2 = 0.58, P = 0.004). Conversely, there was no difference between groups relative to FABP-3 mRNA (P = 0.46) or protein (P = 0.56) levels, oxidative enzymatic a ...
Plasmid replication and control
... Escherichia, Klebsiella, Proteus, Salmonella, and Shigella, but does not transfer to the non enteric gram-negative bacterium Pseudomonas. Different R plasmids with genes for resistance to most antibiotics are known. Many drug-resistant elements on R plasmids, such as those on RI00, are also transpos ...
... Escherichia, Klebsiella, Proteus, Salmonella, and Shigella, but does not transfer to the non enteric gram-negative bacterium Pseudomonas. Different R plasmids with genes for resistance to most antibiotics are known. Many drug-resistant elements on R plasmids, such as those on RI00, are also transpos ...
subunits of succinyl CoA ligase of tomato
... for a- and one coding for the b-subunit of succinyl CoA ligase. These three cDNAs were used to complement the respective Saccharomyces cerevisiae mutants deficient in the a- and b-subunit, demonstrating that they encode functionally active polypeptides. The genes encoding for the subunits were expres ...
... for a- and one coding for the b-subunit of succinyl CoA ligase. These three cDNAs were used to complement the respective Saccharomyces cerevisiae mutants deficient in the a- and b-subunit, demonstrating that they encode functionally active polypeptides. The genes encoding for the subunits were expres ...
Genetic defects causing mitochondrial respiratory
... to treatment of conditions that in any case can show considerable fluctuation in clinical features from week to week. It is widely felt that the development and use of a standardized, objective means of assessing symptoms and response to treatment would be a step forward. The key, meanwhile, to deve ...
... to treatment of conditions that in any case can show considerable fluctuation in clinical features from week to week. It is widely felt that the development and use of a standardized, objective means of assessing symptoms and response to treatment would be a step forward. The key, meanwhile, to deve ...
ISOLATION AND FUNCTIONAL GENETIC EUCALYPTUS Magister Scientiae
... Eucalyptus is an important genus of forest tree species which are widely used as a resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking ...
... Eucalyptus is an important genus of forest tree species which are widely used as a resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking ...
The sequence of human serum albumin cDNA and its expression in
... the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joined to a vector plasmid for direct expression of the mature protein in £ . ...
... the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joined to a vector plasmid for direct expression of the mature protein in £ . ...
Exploring Mouse Protein Function via Multiple Approaches
... the 1st-order predictions in the leave-one-out and ten-fold cross-validations. For the results yielded by the leave-one-out cross-validation, although the similarity-based approach alone achieved an accuracy of 0.8756, it was unable to predict the functions of proteins with no homologues. Comparativ ...
... the 1st-order predictions in the leave-one-out and ten-fold cross-validations. For the results yielded by the leave-one-out cross-validation, although the similarity-based approach alone achieved an accuracy of 0.8756, it was unable to predict the functions of proteins with no homologues. Comparativ ...
Case Study 5 Literature - Department of Chemistry
... CNBr Fragmentation and N-terminal Sequencing—Because the enzyme had been shown to be N-terminally blocked (8), the protein was cleaved with CNBr (21). The resulting peptides were separated by isoelectric focusing in the first dimension and by tricine-SDS-polyacrylamide gel electrophoresis in the sec ...
... CNBr Fragmentation and N-terminal Sequencing—Because the enzyme had been shown to be N-terminally blocked (8), the protein was cleaved with CNBr (21). The resulting peptides were separated by isoelectric focusing in the first dimension and by tricine-SDS-polyacrylamide gel electrophoresis in the sec ...
Primers BSHG06 - National Genetics Reference Laboratories
... This system has been used to design primers for BRCA mutation scanning in the SCOBEC High Throughput Screening facility. The BRCA primer set comprises 79 fragments ranging form 258bp to 509bp covering the entire coding regions of the two genes. To date the primers ...
... This system has been used to design primers for BRCA mutation scanning in the SCOBEC High Throughput Screening facility. The BRCA primer set comprises 79 fragments ranging form 258bp to 509bp covering the entire coding regions of the two genes. To date the primers ...
Transcription Factor EB Controls Metabolic Flexibility during
... TFEB in skeletal muscle increased the expression of mitochondrial enzymes. Subunits of the four respiratory chain complexes and the ATP synthase, as well as genes encoding electron transport and tricarboxylic acid cycle proteins, were induced by TFEB overexpression and were reduced by TFEB deletion ...
... TFEB in skeletal muscle increased the expression of mitochondrial enzymes. Subunits of the four respiratory chain complexes and the ATP synthase, as well as genes encoding electron transport and tricarboxylic acid cycle proteins, were induced by TFEB overexpression and were reduced by TFEB deletion ...
PCR Reagents
... TaqNova DNA polymerase isolated from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy set up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting poly ...
... TaqNova DNA polymerase isolated from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy set up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting poly ...
The regulation of leukemia inhibitory factor
... of LIF mRNA in both tumor cells and fibroblasts, and increase the production and secretion of LIF protein. In turn, LIF promotes proinvasive fibroblast activation and creates an invasive tumor microenvironment that leads to the progression and metastasis of primary tumors [17]. In addition, TGF-β ca ...
... of LIF mRNA in both tumor cells and fibroblasts, and increase the production and secretion of LIF protein. In turn, LIF promotes proinvasive fibroblast activation and creates an invasive tumor microenvironment that leads to the progression and metastasis of primary tumors [17]. In addition, TGF-β ca ...
Nucleus-Encoded Genes for Plastid
... housekeeping functions, photosynthesis, and some anabolic functions. These proteins represent only a small fraction of the proteins that are needed for plastid maintenance and activity: most genes for plastid proteins have been transferred to the nuclear genome, and their products are posttranslatio ...
... housekeeping functions, photosynthesis, and some anabolic functions. These proteins represent only a small fraction of the proteins that are needed for plastid maintenance and activity: most genes for plastid proteins have been transferred to the nuclear genome, and their products are posttranslatio ...
Computational Tools For Protein Modeling
... classification, phylogenetic reconstruction, etc. The conserved regions (motifs) in multiple sequence alignment often have biological significance in terms of structure and function. A correlated mutation between two residue positions can be used to predict a probable physical contact in structure [ ...
... classification, phylogenetic reconstruction, etc. The conserved regions (motifs) in multiple sequence alignment often have biological significance in terms of structure and function. A correlated mutation between two residue positions can be used to predict a probable physical contact in structure [ ...
TAP Express® Kit
... 1 µl of 10 mM dNTP mix 1 µl of 5’ custom oligo or TAP control 5’ primer (30 pmol or 0.4 µg) 1 µl of 3’ custom oligo or TAP control 3’ primer (30 pmol or 0.4 µg) 1 µl of DNA template or TAP control DNA template (10-1000 pg) 1 µl of PCR DNA polymerase (check with supplier for concentration) ...
... 1 µl of 10 mM dNTP mix 1 µl of 5’ custom oligo or TAP control 5’ primer (30 pmol or 0.4 µg) 1 µl of 3’ custom oligo or TAP control 3’ primer (30 pmol or 0.4 µg) 1 µl of DNA template or TAP control DNA template (10-1000 pg) 1 µl of PCR DNA polymerase (check with supplier for concentration) ...
mammalian hibernation: biochemical adaptation
... mammals arise from two main effects of cold on metabolic systems that have been optimized over millions of years of mammalian evolution for function within a narrow temperature window. The first is the differential effect of temperature change on the rates of thousands of cellular reactions that can ...
... mammals arise from two main effects of cold on metabolic systems that have been optimized over millions of years of mammalian evolution for function within a narrow temperature window. The first is the differential effect of temperature change on the rates of thousands of cellular reactions that can ...
Assessment of the mosaic structure in the
... The Helicobacter pylori cagA gene is commonly used as a molecular virulence marker of H. pylori. DNA sequence analysis of the cagA gene has revealed the presence of a conserved 5’-region and a highly variable 3’-region [1-3]. Previous studies have shown that the CagA cytotoxin is directly injected i ...
... The Helicobacter pylori cagA gene is commonly used as a molecular virulence marker of H. pylori. DNA sequence analysis of the cagA gene has revealed the presence of a conserved 5’-region and a highly variable 3’-region [1-3]. Previous studies have shown that the CagA cytotoxin is directly injected i ...
tRNA-derived short RNAs bind to Saccharomyces cerevisiae
... The mode of gene expression regulation by tRNA cleavage is not well understood yet, but similarly to its biogenesis it seems to differ between higher eukaryotes and other organisms. One of the possible regulatory mechanisms is a decrease of tRNA availability. However, it has been shown that during s ...
... The mode of gene expression regulation by tRNA cleavage is not well understood yet, but similarly to its biogenesis it seems to differ between higher eukaryotes and other organisms. One of the possible regulatory mechanisms is a decrease of tRNA availability. However, it has been shown that during s ...
A Survey of Flexible Protein Binding Mechanisms and their
... that flexibility is often more fundamental in the mechanisms of protein association than these pictures lead us to believe. Protein function is not strictly related to the three-dimensional structure of the folded state but to its four-dimensional dynamics governed by an energy landscape.7 The degre ...
... that flexibility is often more fundamental in the mechanisms of protein association than these pictures lead us to believe. Protein function is not strictly related to the three-dimensional structure of the folded state but to its four-dimensional dynamics governed by an energy landscape.7 The degre ...
FOG-1 - Blood Journal
... morpholino directed against the homolog to murine FOG-1 resulted in embryos with a large pericardial effusion and a loopingdeficient heart tube. This looping defect could be rescued by coinjection of cRNA encoding zebrafish or murine FOG-1.24 Together, prior studies indicate that FOG-1 positively an ...
... morpholino directed against the homolog to murine FOG-1 resulted in embryos with a large pericardial effusion and a loopingdeficient heart tube. This looping defect could be rescued by coinjection of cRNA encoding zebrafish or murine FOG-1.24 Together, prior studies indicate that FOG-1 positively an ...
Fragile X-associated disorders (FXd)
... read that these could be features of a neurological condition that occurs in some male (and less frequently, female) Fragile X carriers. Within a short time period, the family had gone from never having heard about FXD to quickly learning that multiple family members were directly affected. Even tho ...
... read that these could be features of a neurological condition that occurs in some male (and less frequently, female) Fragile X carriers. Within a short time period, the family had gone from never having heard about FXD to quickly learning that multiple family members were directly affected. Even tho ...