NUCLEOTIDES AND NUCLEIC ACIDS
... Both DNA and RNA contain two major purine bases, adenine (A) and guanine (G), and two major pyrimidines. In both DNA and RNA one of the pyrimidines is cytosine (C), but the second major pyrimidine is not the same in both: it is thymine (T) in DNA and uracil (U) in RNA. Only rarely does thymine occur ...
... Both DNA and RNA contain two major purine bases, adenine (A) and guanine (G), and two major pyrimidines. In both DNA and RNA one of the pyrimidines is cytosine (C), but the second major pyrimidine is not the same in both: it is thymine (T) in DNA and uracil (U) in RNA. Only rarely does thymine occur ...
powerpoint
... • Relatively short DNA fragments can be sequenced by the dideoxy chain termination method, the first automated method to be employed • Modified nucleotides called dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with a distinct fluo ...
... • Relatively short DNA fragments can be sequenced by the dideoxy chain termination method, the first automated method to be employed • Modified nucleotides called dideoxyribonucleotides (ddNTP) attach to synthesized DNA strands of different lengths • Each type of ddNTP is tagged with a distinct fluo ...
Genetic Engineering for Improved Xylose Fermentation by Yeasts
... A putative xylose transporter gene from P. stipitis, PsSTU1, has been cloned recently. This gene can confer high-affinity uptake of glucose and growth to a S. cerevisiae hxt1-7 strain [60]. The deduced amino acid sequence shows 54% identity to the HXT glucose transporters of S. cerevisiae. When PsST ...
... A putative xylose transporter gene from P. stipitis, PsSTU1, has been cloned recently. This gene can confer high-affinity uptake of glucose and growth to a S. cerevisiae hxt1-7 strain [60]. The deduced amino acid sequence shows 54% identity to the HXT glucose transporters of S. cerevisiae. When PsST ...
Altered pathogenicity, immunity, tissue tropism and 3` genome
... new host, the embryo, reflected by more efficient replication and higher lethality to the embryo. In addition, during passages in embryonated eggs, recombination between virus subpopulations, and accumulation of mutations in the S1 region can lead to the formation of attenuated viruses [19]. The imp ...
... new host, the embryo, reflected by more efficient replication and higher lethality to the embryo. In addition, during passages in embryonated eggs, recombination between virus subpopulations, and accumulation of mutations in the S1 region can lead to the formation of attenuated viruses [19]. The imp ...
Nucleic Acids Research
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
... most of these have been of the G-+T type when comparing the pTi T37 to the pTi Ach5 sequence. Without quantitative comparison of transcription levels from the two tmr loci, it is not possible to assess the overall effects of these base changes on relative promoter strength. Heidekamp et al. found tw ...
Induction of y-Gultamyl Transpeptidase by a Thyroid Hormone in
... It has been known that steroid and thyroid hormones and retinoic acids coordinate complex events involved in development, differentiation, and physiological response to diverse stimuli. Furthermore, recent studies of steroid receptors have led to the identification of a superfamily of ligand-inducib ...
... It has been known that steroid and thyroid hormones and retinoic acids coordinate complex events involved in development, differentiation, and physiological response to diverse stimuli. Furthermore, recent studies of steroid receptors have led to the identification of a superfamily of ligand-inducib ...
Supplementary Text S1: Detailed PCR Protocols
... of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and DDX58, total RNAs were extracted from DCs from the same donor studied in for the single cell RT described below and were diluted to approximately 1 copy as determined by the PCR re ...
... of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and DDX58, total RNAs were extracted from DCs from the same donor studied in for the single cell RT described below and were diluted to approximately 1 copy as determined by the PCR re ...
Reducing Rice Seed Storage Protein Accumulation Leads to
... composition with the most prominent difference being the Cys content. The class I prolamins (e.g, RM2 and RM4) are poor in Cys (one Cys residue/molecule), the class II prolamins (e.g, RM9) contain the highest number of Cys (nine Cys/molecule), and the class III prolamins (e.g, RM1) contain an inter ...
... composition with the most prominent difference being the Cys content. The class I prolamins (e.g, RM2 and RM4) are poor in Cys (one Cys residue/molecule), the class II prolamins (e.g, RM9) contain the highest number of Cys (nine Cys/molecule), and the class III prolamins (e.g, RM1) contain an inter ...
Synonymous codons are not the same with
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
Characterization of Low Molecular Weight Glutenin Subunits by
... these DNA-derived sequences are slightly different from the Nterminal sequence of the "aggregated gliadin" subunit (LMWGS) described by Shewry et al (1983) and the N-terminal sequence of a major LMW-GS reported by Kasarda et al (1988). Tao and Kasarda (1989) found this latter sequence to be the majo ...
... these DNA-derived sequences are slightly different from the Nterminal sequence of the "aggregated gliadin" subunit (LMWGS) described by Shewry et al (1983) and the N-terminal sequence of a major LMW-GS reported by Kasarda et al (1988). Tao and Kasarda (1989) found this latter sequence to be the majo ...
Full-Text PDF
... and citrate were also highly correlated with many important regulators of ripening in an independent study focused on early tomato fruit development [28]. As opposed to the observations for cherimoya, malic acid increases in other fruits such as grape (Vitis vinifera) at earlier stages and then decr ...
... and citrate were also highly correlated with many important regulators of ripening in an independent study focused on early tomato fruit development [28]. As opposed to the observations for cherimoya, malic acid increases in other fruits such as grape (Vitis vinifera) at earlier stages and then decr ...
Metabolism of sucrose and its five isomers by
... coli PEP43 expresses no phospho-β-glucosidases because the cel and bglGFB operons have been deleted, bglA is disrupted by Tn10 and the asc operon is cryptic. Cells of E. coli PEP43(pCB4.11) (" 25 g wet weight) were resuspended with 40 ml TNM buffer, and the organisms were disrupted by 2i1n5 min soni ...
... coli PEP43 expresses no phospho-β-glucosidases because the cel and bglGFB operons have been deleted, bglA is disrupted by Tn10 and the asc operon is cryptic. Cells of E. coli PEP43(pCB4.11) (" 25 g wet weight) were resuspended with 40 ml TNM buffer, and the organisms were disrupted by 2i1n5 min soni ...
Construction of in vivo infectious clones of PRSV P-YK and W-CI
... 35S promoter. The mutated plasmid was sequenced to verify the deletion and ligated with other viral sequence from pT3YKFN1 to obtain the final construct of p35SPYK that contained a full-length cDNA of P-YK and a 35S promoter and a nos terminator. The strategy for the construction of in vivo infectio ...
... 35S promoter. The mutated plasmid was sequenced to verify the deletion and ligated with other viral sequence from pT3YKFN1 to obtain the final construct of p35SPYK that contained a full-length cDNA of P-YK and a 35S promoter and a nos terminator. The strategy for the construction of in vivo infectio ...
Molecular Cloning and Expression Analysis of IgD in Nile
... Igs exist in all species of fish. Different from the IgM, which was the only Ig molecule found to be common to all species of fish, the IgD gene has been only shown to be present in some fish species [1–7]. Previous studies show that IgD is an ancient Ig molecule, so identification of the IgD gene i ...
... Igs exist in all species of fish. Different from the IgM, which was the only Ig molecule found to be common to all species of fish, the IgD gene has been only shown to be present in some fish species [1–7]. Previous studies show that IgD is an ancient Ig molecule, so identification of the IgD gene i ...
Experimental Analysis of the Rice Mitochondrial
... Mitochondria in rice (Oryza sativa) are vital in expanding our understanding of the cellular response to reoxygenation of tissues after anaerobiosis, the crossroads of carbon and nitrogen metabolism, and the role of respiratory energy generation in cytoplasmic male sterility. We have combined densit ...
... Mitochondria in rice (Oryza sativa) are vital in expanding our understanding of the cellular response to reoxygenation of tissues after anaerobiosis, the crossroads of carbon and nitrogen metabolism, and the role of respiratory energy generation in cytoplasmic male sterility. We have combined densit ...
Fulltext - Jultika
... enzymatically active, reducing 2E-enoyl-CoAs to acyl-CoAs in an NADPH-dependent manner. Altogether, our data showed that NRBF-1 is a mitochondrial 2-enoyl-CoA reductase/2-enoyl thioester reductase (MECR/ETR1), the human functional counterpart of yeast Etr1p. In addition, MECR was also isolated from ...
... enzymatically active, reducing 2E-enoyl-CoAs to acyl-CoAs in an NADPH-dependent manner. Altogether, our data showed that NRBF-1 is a mitochondrial 2-enoyl-CoA reductase/2-enoyl thioester reductase (MECR/ETR1), the human functional counterpart of yeast Etr1p. In addition, MECR was also isolated from ...
Inhibition of Wound-Induced Accumulation of
... rRNA cDNA (data not shown). The 28S rRNA gene was used as a loading control. Each experiment was independently repeated at least three times. The film was exposed overnight for 14 h at 280°C. The signals on the x-ray films were quantified densitometrically with a densitometer (model GS-670, Bio-Rad) ...
... rRNA cDNA (data not shown). The 28S rRNA gene was used as a loading control. Each experiment was independently repeated at least three times. The film was exposed overnight for 14 h at 280°C. The signals on the x-ray films were quantified densitometrically with a densitometer (model GS-670, Bio-Rad) ...
WS Procs 975 x 65
... representation to the Dependency Pattern Sets (DPS) representation. DPS representation is a generalized model of string representation, which can model adjacent nucleotide dependency. Although it has a lower descriptive power than SPSP representation, experimental results on real biological data sho ...
... representation to the Dependency Pattern Sets (DPS) representation. DPS representation is a generalized model of string representation, which can model adjacent nucleotide dependency. Although it has a lower descriptive power than SPSP representation, experimental results on real biological data sho ...
ETS-dependent regulation of a distal Gata4 cardiac enhancer
... mice lacking GATA4 in the myocardium display defective cardiomyocyte proliferation and embryonic lethality (Rojas et al., 2008; Zeisberg et al., 2005). Mice lacking Gata4 in the endothelium have defective endocardial cushion development and die around E12.5 of apparent heart failure (Rivera-Felician ...
... mice lacking GATA4 in the myocardium display defective cardiomyocyte proliferation and embryonic lethality (Rojas et al., 2008; Zeisberg et al., 2005). Mice lacking Gata4 in the endothelium have defective endocardial cushion development and die around E12.5 of apparent heart failure (Rivera-Felician ...
Pseudomon-1 motif
... likely partition the three SAM-IV RNAs into a different cluster from the other COG0520 UTRs, which reduces spurious sequences in the cluster. It should be noted, however, that one drawback to BLAST-based sequence clustering is that the accuracy of BLAST searches accuracy may be limited. Our frequent ...
... likely partition the three SAM-IV RNAs into a different cluster from the other COG0520 UTRs, which reduces spurious sequences in the cluster. It should be noted, however, that one drawback to BLAST-based sequence clustering is that the accuracy of BLAST searches accuracy may be limited. Our frequent ...
Evolution of codon usage bias in Drosophila
... that mutation bias is playing a large role in maintaining codon usage in Drosophila. In the absence of such bias, we are left with some form of selection as an explanation. Selection for Codon Usage. The most plausible and welldocumented selection-based explanation for codon usage bias is selection ...
... that mutation bias is playing a large role in maintaining codon usage in Drosophila. In the absence of such bias, we are left with some form of selection as an explanation. Selection for Codon Usage. The most plausible and welldocumented selection-based explanation for codon usage bias is selection ...
Protein kinases - Institut de recherches cliniques de Montréal
... 1906: Discovery of the first phosphorylated protein (Vitellin) by Phoebus A. Levene 1933: With Fritz Lipmann, Levene discovers a phosphoserine in the protein casein 1954: First description of a kinase activity on casein 1955: Fischer & Krebs and Sutherland demonstrate that conversion between phospho ...
... 1906: Discovery of the first phosphorylated protein (Vitellin) by Phoebus A. Levene 1933: With Fritz Lipmann, Levene discovers a phosphoserine in the protein casein 1954: First description of a kinase activity on casein 1955: Fischer & Krebs and Sutherland demonstrate that conversion between phospho ...
for growth. fermentation end products and genes required growth of
... of chloramphenicol per ml, 75 mg of spectinomycin per ml, 1 mg of erythromycin per ml and 25 mg of lincomycin per ml, 10 mg of tetracycline per ml, and 0.5 mg of phleomycin per ml. Construction of fnr (3, 20), DresE (34), resD (34), and DresDE (20, 34) mutants was previously described. Plasmid pJL62 ...
... of chloramphenicol per ml, 75 mg of spectinomycin per ml, 1 mg of erythromycin per ml and 25 mg of lincomycin per ml, 10 mg of tetracycline per ml, and 0.5 mg of phleomycin per ml. Construction of fnr (3, 20), DresE (34), resD (34), and DresDE (20, 34) mutants was previously described. Plasmid pJL62 ...