Genomics Insights esTs from seeds to Assist the selective Breeding
... super-family,10 which is conserved among a large number of species. This molecule is used to induce skin tumors in mouse (a model for cancer investigations),10 and is responsible for strong digestive poisoning upon ingestion. This has raised concerns about the health of people that may come into con ...
... super-family,10 which is conserved among a large number of species. This molecule is used to induce skin tumors in mouse (a model for cancer investigations),10 and is responsible for strong digestive poisoning upon ingestion. This has raised concerns about the health of people that may come into con ...
Identification and removal of colanic acid from plasmid DNA
... LPS in plasmid DNA isolated from E.coli, including commercially available kits.10 These methods generally decrease the levels of polysaccharides linked to lipid A. However, lipid A is present in only a small fraction of the capsular polysaccharides of E. coli comprising group I.11,12 Several other m ...
... LPS in plasmid DNA isolated from E.coli, including commercially available kits.10 These methods generally decrease the levels of polysaccharides linked to lipid A. However, lipid A is present in only a small fraction of the capsular polysaccharides of E. coli comprising group I.11,12 Several other m ...
Zebra Finch Glucokinase Containing Two Homologous Halves Is an
... to the C-terminus of hexokinase II N-half. “Original” Zebra finch glucokinase C-terminus has been reconstructed by us. It is encoded by newly mapped exon. That “new” exon has been found in chromosome 22: from the nucleotide 132,945 to the nucleotide 133,088 (see Figure 2). There is a donor splicing ...
... to the C-terminus of hexokinase II N-half. “Original” Zebra finch glucokinase C-terminus has been reconstructed by us. It is encoded by newly mapped exon. That “new” exon has been found in chromosome 22: from the nucleotide 132,945 to the nucleotide 133,088 (see Figure 2). There is a donor splicing ...
Sequence and phylogenetic analysis of the gp200 protein of
... sequences derived from 17 Taiwanese samples were identical or nearly identical to each other (99.9∼100% identities), even though the canine blood samples were obtained from different locations. The three representative sequences obtained from this study have been deposited in the GenBank database un ...
... sequences derived from 17 Taiwanese samples were identical or nearly identical to each other (99.9∼100% identities), even though the canine blood samples were obtained from different locations. The three representative sequences obtained from this study have been deposited in the GenBank database un ...
International Journal of Antimicrobial Agents Mechanism of drug
... pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated with greater frequency from patients with cholera-like illness, many of which display multiple drug resistance [5–8]. There are se ...
... pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated with greater frequency from patients with cholera-like illness, many of which display multiple drug resistance [5–8]. There are se ...
Biology - Kenyon College
... Acetate and formate are major fermentation products of Escherichia coli. Below pH 7, the balance shifts to lactate; an oversupply of acetate or formate retards growth. E. coli W3110 was grown with aeration in potassium-modified Luria broth buffered at pH 6.7 in the presence or absence of added aceta ...
... Acetate and formate are major fermentation products of Escherichia coli. Below pH 7, the balance shifts to lactate; an oversupply of acetate or formate retards growth. E. coli W3110 was grown with aeration in potassium-modified Luria broth buffered at pH 6.7 in the presence or absence of added aceta ...
Identity elements in tRNA-mediated transcription
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF
... organisms. It is based on the concept of the proteome as a complete set of proteins produced by a given cell or organism under a defined conditions. Proteins present the major constituent of living cells and participate in almost every biological process in all organisms. Therefore, a comprehensive ...
... organisms. It is based on the concept of the proteome as a complete set of proteins produced by a given cell or organism under a defined conditions. Proteins present the major constituent of living cells and participate in almost every biological process in all organisms. Therefore, a comprehensive ...
PCR
... Fidelity of Taq or error rate is: 1 base misincorporation per 104 nucleotides polymerized for a 400 bp fragment amplied 106 fold (=20 cycles) results in in about 33% of the products carrying a mutation (thus should sequence several PCR amplimers to determine consensus) ...
... Fidelity of Taq or error rate is: 1 base misincorporation per 104 nucleotides polymerized for a 400 bp fragment amplied 106 fold (=20 cycles) results in in about 33% of the products carrying a mutation (thus should sequence several PCR amplimers to determine consensus) ...
Novel Expression Pattern of Cytosolic Gln
... III in Fig. 3, A and F), where Frankia vesicles have differentiated and nitrogen fixation takes place; and the zone of senescence (zone IV in Fig. 3F), where Frankia material is degraded by the plant, according to terminology established by Ribeiro et al. (1995). Frankiainfected tissue was distingui ...
... III in Fig. 3, A and F), where Frankia vesicles have differentiated and nitrogen fixation takes place; and the zone of senescence (zone IV in Fig. 3F), where Frankia material is degraded by the plant, according to terminology established by Ribeiro et al. (1995). Frankiainfected tissue was distingui ...
Coordination of the maize transcriptome by a conserved circadian
... consequence, C4 crop plants assimilate biomass more efficiently than C3 plants at the high temperatures typical of agricultural settings [30]. Previous studies have shown the circadian clock serves to coordinate expression of genes encoding the photosynthesis apparatus in plants that carry out C3 ph ...
... consequence, C4 crop plants assimilate biomass more efficiently than C3 plants at the high temperatures typical of agricultural settings [30]. Previous studies have shown the circadian clock serves to coordinate expression of genes encoding the photosynthesis apparatus in plants that carry out C3 ph ...
Identification and Analysis of Dicer Associated Proteins in
... complementarity, and either promote translational inhibition or degradation of their target mRNAs (for review see: (Filipowicz et al., 2005)). Foremost, the 5’ end of the miRNA, e.g. nt 2 to 8, shows almost perfect complementarity to its target site, and was termed a “seed region”. Functional miRNA ...
... complementarity, and either promote translational inhibition or degradation of their target mRNAs (for review see: (Filipowicz et al., 2005)). Foremost, the 5’ end of the miRNA, e.g. nt 2 to 8, shows almost perfect complementarity to its target site, and was termed a “seed region”. Functional miRNA ...
Cambridge Workshop November 2008
... 3. Flag data types in uncurated publications 4. Submit revisions to gene models 5. Work with FlyBase curators to improve specific areas 6. Annotate / clean up 'gene orphans’ 7. Use FlyBase symbols in your papers 8. Provide accurate references for reagents in your papers 9. Declare FlyBase release nu ...
... 3. Flag data types in uncurated publications 4. Submit revisions to gene models 5. Work with FlyBase curators to improve specific areas 6. Annotate / clean up 'gene orphans’ 7. Use FlyBase symbols in your papers 8. Provide accurate references for reagents in your papers 9. Declare FlyBase release nu ...
... b) Regulation of glycolysis d) Regulation of mRNA synthesis. 1. Allosteric effects refer to the fact that a protein/enzyme has two structures. 2. One structure is “active” (R-state) the other structure is “inactive” (T-state). 3. The two states are in equilibrium with each other, so the system can b ...
Determination of Optimal Glucose Concentrations
... 2(B)). In this case, the difference between two optimal glucose concentrations was smaller than that of MMY2SUCSTA since the slope of the specific secretion rate of MMY2SUCSTA-I was larger in the region of low glucose concentration. This calculation was performed using only eqn (2) for specific expr ...
... 2(B)). In this case, the difference between two optimal glucose concentrations was smaller than that of MMY2SUCSTA since the slope of the specific secretion rate of MMY2SUCSTA-I was larger in the region of low glucose concentration. This calculation was performed using only eqn (2) for specific expr ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... that is generated in plastids has a single known fate, the formation of fatty acids (Ohlrogge et al., 1979; Stumpf, 1987; Harwood, 1988); in contrast, cytosolic malonyl-COA is not utilized for de novo fatty acid biosynthesis but for the synthesis of a variety of phytochemicals (Com, 1981; Nikolau, e ...
... that is generated in plastids has a single known fate, the formation of fatty acids (Ohlrogge et al., 1979; Stumpf, 1987; Harwood, 1988); in contrast, cytosolic malonyl-COA is not utilized for de novo fatty acid biosynthesis but for the synthesis of a variety of phytochemicals (Com, 1981; Nikolau, e ...
Comparison of Genes Encoding Enzymes of Sterol Biosynthesis
... Sterols are ubiquitous membrane lipid components found in eukaryotic organisms. Sterols are known to serve novel functions in eukaryotes. Sterol biosynthesis pathways differ among fungi, plants and vertebrates. Lipid biosynthesis has been extensively studied in the model yeast Saccharomyces cerevisi ...
... Sterols are ubiquitous membrane lipid components found in eukaryotic organisms. Sterols are known to serve novel functions in eukaryotes. Sterol biosynthesis pathways differ among fungi, plants and vertebrates. Lipid biosynthesis has been extensively studied in the model yeast Saccharomyces cerevisi ...
How cohesin and CTCF cooperate in regulating gene expression
... identified as a protein that controls gene expression. In this study, Dorsett and colleagues used the gypsy element, a transcriptional insulator sequence, to search for genes mediating promoter-enhancer interactions. Heterozygous mutations in Nipped-B were found to magnify the enhancer-blocking effe ...
... identified as a protein that controls gene expression. In this study, Dorsett and colleagues used the gypsy element, a transcriptional insulator sequence, to search for genes mediating promoter-enhancer interactions. Heterozygous mutations in Nipped-B were found to magnify the enhancer-blocking effe ...
Prying Into Prions - Colorado Parks and Wildlife
... moose. It belongs to a family of diseases known as transmissible spongiform encephalopathies or prion diseases. The disease attacks the brains of infected deer, elk and moose, causing the animals to become emaciated, display abnormal behavior and lose coordination, and eventually die. Chronic Wastin ...
... moose. It belongs to a family of diseases known as transmissible spongiform encephalopathies or prion diseases. The disease attacks the brains of infected deer, elk and moose, causing the animals to become emaciated, display abnormal behavior and lose coordination, and eventually die. Chronic Wastin ...
Automated extraction of mutation data from the literature: application
... terms with their true contextual meanings. For example, the term ‘T47D’ will be ignored if it is found close to the terms ‘cell line’, ‘tumour’, ‘tumor’ or ‘cancer’. This list will have to be updated manually as other point mutation-like terms are detected in the documents subsequently processed. Ho ...
... terms with their true contextual meanings. For example, the term ‘T47D’ will be ignored if it is found close to the terms ‘cell line’, ‘tumour’, ‘tumor’ or ‘cancer’. This list will have to be updated manually as other point mutation-like terms are detected in the documents subsequently processed. Ho ...
Methodology for predicting semantic annotations of protein
... 3 Background 3.1 Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.1.1 Protein synthesis . . . . . . . . . . . . . . . . . . 3.1.2 Protein structure . . . . . . . . . . . . . . . . . . 3.1.3 Protein folding . . . . . . . . . . . . . . . . . . . 3.1.4 Protein domains and motifs . . . . ...
... 3 Background 3.1 Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.1.1 Protein synthesis . . . . . . . . . . . . . . . . . . 3.1.2 Protein structure . . . . . . . . . . . . . . . . . . 3.1.3 Protein folding . . . . . . . . . . . . . . . . . . . 3.1.4 Protein domains and motifs . . . . ...