Formation of Monoterpenes in Antirrhinum majus
... apex, inflorescences, young buds, and flowers at different developmental stages as well as fertilized and unfertilized carpels. This search revealed three different GGPPS-like contigs. Of these, we determined experimentally that one was not expressed in petals (data not shown), and a second sequence ...
... apex, inflorescences, young buds, and flowers at different developmental stages as well as fertilized and unfertilized carpels. This search revealed three different GGPPS-like contigs. Of these, we determined experimentally that one was not expressed in petals (data not shown), and a second sequence ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
... phylogenetically analyzed. CCK gene and protein expression levels in the different gut segments were measured 3 h and 72 h after feeding, by quantitative real-time RT-PCR and Western blot, respectively. Moreover, endocrine CCK cells were immunoistochemically detected. Fasting induced a significant d ...
... phylogenetically analyzed. CCK gene and protein expression levels in the different gut segments were measured 3 h and 72 h after feeding, by quantitative real-time RT-PCR and Western blot, respectively. Moreover, endocrine CCK cells were immunoistochemically detected. Fasting induced a significant d ...
Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine
... the growth of S. aureus in a mammal. Our work provides new insights into the discovery and design of novel antimicrobial agents against bacterial pathogens. from natural ligands to avoid cellular efflux or chemical modification, it is important to consider that these chemical differences will potent ...
... the growth of S. aureus in a mammal. Our work provides new insights into the discovery and design of novel antimicrobial agents against bacterial pathogens. from natural ligands to avoid cellular efflux or chemical modification, it is important to consider that these chemical differences will potent ...
EVOLUTION OF A REGULATED OPERON IN THE LABORATORY
... In contrast, the understanding of evolution, or “how things got to be this way,” has depended upon fragmentary fossil evidence and upon comparisons of existing organisms with each other and with their extinct ancestors. Based upon an enormous number of observations at the anatomical and molecular le ...
... In contrast, the understanding of evolution, or “how things got to be this way,” has depended upon fragmentary fossil evidence and upon comparisons of existing organisms with each other and with their extinct ancestors. Based upon an enormous number of observations at the anatomical and molecular le ...
ARTICLE Functional analysis of mutations in SLC7A9, and genotype
... Mutations in SLC3A1, located on chromosome 2p16.3–21 and encoding the bo,+ transporter-related protein rBAT, cause only Type I cystinuria (2,6). The gene causing non-Type I cystinuria was assigned by linkage to chromosome 19q12–13.1 (7,8), and was identified as SLC7A9 (3), whose protein product (bo, ...
... Mutations in SLC3A1, located on chromosome 2p16.3–21 and encoding the bo,+ transporter-related protein rBAT, cause only Type I cystinuria (2,6). The gene causing non-Type I cystinuria was assigned by linkage to chromosome 19q12–13.1 (7,8), and was identified as SLC7A9 (3), whose protein product (bo, ...
In situ hybridization
... that has performed in situ hybridization with RNA probes (riboprobes) knows they can be very frustrating to work with. Furthermore, and this is an important point, if somewhat counter-intuitive, it can be shown that because of their long size (100s of base-pairs as opposed to oligonucleotide probes ...
... that has performed in situ hybridization with RNA probes (riboprobes) knows they can be very frustrating to work with. Furthermore, and this is an important point, if somewhat counter-intuitive, it can be shown that because of their long size (100s of base-pairs as opposed to oligonucleotide probes ...
Comparative proteomic network signatures in seminal plasma of
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
... Background: Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the fema ...
Two Oxidosqualene Cyclases Responsible for
... reported to be multifunctional (Kushiro et al., 2000; Morita et al., 2000; Basyuni et al., 2006). For a few plant species, more than one OSC has been characterized, so that the suite of OSCs accounted for at least part of the triterpenoid profile in those species (Morita et al., 1997, 2000; Hayashi ...
... reported to be multifunctional (Kushiro et al., 2000; Morita et al., 2000; Basyuni et al., 2006). For a few plant species, more than one OSC has been characterized, so that the suite of OSCs accounted for at least part of the triterpenoid profile in those species (Morita et al., 1997, 2000; Hayashi ...
Gene Section CYP2A6 (cytochrome P450, family 2, subfamily A, polypeptide 6)
... CYP2A6 is an enzyme responsible for the metabolism of clinically used pharmaceuticals such as coumarin and valproic acid, several carcinogens such as 4(methylnitrosamine)-1-(3-pyridyl)-1-butanone and aflatoxin B1, nicotine and for the bioactivation process of tegafur to 5-FU. The formation of 5-FU f ...
... CYP2A6 is an enzyme responsible for the metabolism of clinically used pharmaceuticals such as coumarin and valproic acid, several carcinogens such as 4(methylnitrosamine)-1-(3-pyridyl)-1-butanone and aflatoxin B1, nicotine and for the bioactivation process of tegafur to 5-FU. The formation of 5-FU f ...
The Polymerase Chain Reaction
... reverse transcribe mRNA into DNA before the PCR begins When DNA is made this way it is referred to as cDNA – RT-PCR is often used in combination with real-time PCR to measure levels of gene expression Reverse transcription ...
... reverse transcribe mRNA into DNA before the PCR begins When DNA is made this way it is referred to as cDNA – RT-PCR is often used in combination with real-time PCR to measure levels of gene expression Reverse transcription ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal ca ...
... primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal ca ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
Studies on the structure and function of 16S ribosomal RNA using
... base-paired, providing strong general support for the secondary structure model. (ii) Candidates for possible tertiary interactions are identified as adenine residues that are unpaired in the model and unreactive under native conditions but reactive under quasi-secondary conditions. We find 57 such ...
... base-paired, providing strong general support for the secondary structure model. (ii) Candidates for possible tertiary interactions are identified as adenine residues that are unpaired in the model and unreactive under native conditions but reactive under quasi-secondary conditions. We find 57 such ...
Evolution and Function of the Plant Cell Wall
... (PF01501; 345 amino acids long) domain (referred to as the GT8 domain throughout this article; for details, see “Materials and Methods”) as the query. This HMMER search, using an E-value cutoff of #1e-2, identified 378 nonidentical GT8 domains in 378 proteins from the 15 genomes, which are herein ref ...
... (PF01501; 345 amino acids long) domain (referred to as the GT8 domain throughout this article; for details, see “Materials and Methods”) as the query. This HMMER search, using an E-value cutoff of #1e-2, identified 378 nonidentical GT8 domains in 378 proteins from the 15 genomes, which are herein ref ...
Determination of protein regions responsible for interactions of
... 1983 [1] and the subsequent discoveries of additional organic components of the enamel extracellular matrix, including ameloblastin [2], enamelin [3] and amelotin [4], our understanding of enamel formation has advanced significantly. The expression of these structural proteins remains relatively uni ...
... 1983 [1] and the subsequent discoveries of additional organic components of the enamel extracellular matrix, including ameloblastin [2], enamelin [3] and amelotin [4], our understanding of enamel formation has advanced significantly. The expression of these structural proteins remains relatively uni ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... Sequences from operons rrnA and rrnB were amplified in PCR primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rr ...
... Sequences from operons rrnA and rrnB were amplified in PCR primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rr ...
View/Open - VUW research archive - Victoria University of Wellington
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
Arabidopsis 3-ketoacyl-CoA synthase 9 is involved in the synthesis
... suberins, phospholipids, sphingolipids, and seed oils in the Brassicaceae. These lipids are involved in various functions, such as acting as protective barriers between plants and the environment, impermeable barriers to water and ions, energy-storage compounds in seeds, structural components of mem ...
... suberins, phospholipids, sphingolipids, and seed oils in the Brassicaceae. These lipids are involved in various functions, such as acting as protective barriers between plants and the environment, impermeable barriers to water and ions, energy-storage compounds in seeds, structural components of mem ...
Two Arabidopsis Genes (IPMS1 and IPMS2
... Heterologous expression of the Arabidopsis (Arabidopsis thaliana) IPMS1 (At1g18500) and IPMS2 (At1g74040) cDNAs in Escherichia coli yields isopropylmalate synthases (IPMSs; EC 2.3.3.13). These enzymes catalyze the first dedicated step in leucine (Leu) biosynthesis, an aldol-type condensation of acet ...
... Heterologous expression of the Arabidopsis (Arabidopsis thaliana) IPMS1 (At1g18500) and IPMS2 (At1g74040) cDNAs in Escherichia coli yields isopropylmalate synthases (IPMSs; EC 2.3.3.13). These enzymes catalyze the first dedicated step in leucine (Leu) biosynthesis, an aldol-type condensation of acet ...