Thermodynamic analysis of the unfolding and stability of the dimeric
... complexes and is also involved in a variety of DNA metabolic events, such as replication, transcription and transposition [11,12]. Its ability to repair DNA [13,14] and to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of ...
... complexes and is also involved in a variety of DNA metabolic events, such as replication, transcription and transposition [11,12]. Its ability to repair DNA [13,14] and to prevent DNA duplex melting [7] has also been described. HU proteins from several species of bacillus growing in environments of ...
PTC Taster Lab Student`s Guide
... variation besides sensitivity to PTC, including SNP association with certain diseases. In some cases SNPs may directly influence physiology (e.g. by directly altering the coding sequence for a gene), and in other cases they simply serve as biological markers that are associated with genetic traits ( ...
... variation besides sensitivity to PTC, including SNP association with certain diseases. In some cases SNPs may directly influence physiology (e.g. by directly altering the coding sequence for a gene), and in other cases they simply serve as biological markers that are associated with genetic traits ( ...
Gonococcal outer-membrane protein PIB
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
Fulltext PDF - Indian Academy of Sciences
... mpbio.dundee.ac.uk/www-jpred/). All the changes, except for Rv1773c, were predicted in the loop region. Only Rv1773c was found to be in the helix state. In general, the loop regions of proteins are involved in functional activity (Wehbi et al. 2007). Since it is known that one amino acid alteration ...
... mpbio.dundee.ac.uk/www-jpred/). All the changes, except for Rv1773c, were predicted in the loop region. Only Rv1773c was found to be in the helix state. In general, the loop regions of proteins are involved in functional activity (Wehbi et al. 2007). Since it is known that one amino acid alteration ...
Cardiac troponin I gene knockout - University of Wisconsin–Madison
... common responses to cardiac insufficiency.21,22 Heart tissue sections prepared from cTnI–/– animals and stained with hematoxylin and eosin revealed neither hypertrophy nor ventricular dilation even in 18-day-old animals with advanced symptoms (not shown). Gomori’s trichrome staining for fibrosis als ...
... common responses to cardiac insufficiency.21,22 Heart tissue sections prepared from cTnI–/– animals and stained with hematoxylin and eosin revealed neither hypertrophy nor ventricular dilation even in 18-day-old animals with advanced symptoms (not shown). Gomori’s trichrome staining for fibrosis als ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Developing a protocol for electroporation of N. pharaonis............... 141 ...
... Developing a protocol for electroporation of N. pharaonis............... 141 ...
Popped Secret: The Mysterious Origin of Corn HHMI BioInteractive
... varieties from throughout Mexico. What did their analysis reveal? Select all that apply. A. That teosinte and maize have the same number of chromosomes. B. That maize originated from a variety of teosinte that existed about 9,000 years ago. C. That maize and teosinte could interbreed to produce viab ...
... varieties from throughout Mexico. What did their analysis reveal? Select all that apply. A. That teosinte and maize have the same number of chromosomes. B. That maize originated from a variety of teosinte that existed about 9,000 years ago. C. That maize and teosinte could interbreed to produce viab ...
The Art of Multiple Sequence Alignment in R
... metrics in order to accomplish a combination of both structural alignment and evolutionary alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for seq ...
... metrics in order to accomplish a combination of both structural alignment and evolutionary alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for seq ...
VTC4 Is a Bifunctional Enzyme That Affects
... T-DNA mutant database and were verified by sequencing of amplified PCR products. The vtc4-2 mutant contains two tandem T-DNA insertions occurring 1,341 nucleotides from the start site of translation. The vtc4-3 and vtc4-4 mutants contain T-DNA insertions within the seventh exon and second intron, re ...
... T-DNA mutant database and were verified by sequencing of amplified PCR products. The vtc4-2 mutant contains two tandem T-DNA insertions occurring 1,341 nucleotides from the start site of translation. The vtc4-3 and vtc4-4 mutants contain T-DNA insertions within the seventh exon and second intron, re ...
Genetic Disorders Leading to Hypoglycaemia
... prolactin, and TSH. The LHX4 gene on chromosome 1q25.2 encodes the LHX4 protein, which is needed for the expression of other pituitary transcription factors including LHX3 (gene on chromosome 9q34.3). Mutations of LHX4 lead to autosomal dominant CPHD. The PROP-1 protein has both DNA binding and tran ...
... prolactin, and TSH. The LHX4 gene on chromosome 1q25.2 encodes the LHX4 protein, which is needed for the expression of other pituitary transcription factors including LHX3 (gene on chromosome 9q34.3). Mutations of LHX4 lead to autosomal dominant CPHD. The PROP-1 protein has both DNA binding and tran ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
Identification of a Vinyl Reductase Gene for
... (Parham and Rebeiz, 1995). However, at this time, it is unclear whether different enzymes or a single enzyme are responsible for the reduction of the 8-vinyl group of various intermediate molecules of chlorophyll biosynthesis. Enzymatic studies with purified protein(s) would facilitate the identific ...
... (Parham and Rebeiz, 1995). However, at this time, it is unclear whether different enzymes or a single enzyme are responsible for the reduction of the 8-vinyl group of various intermediate molecules of chlorophyll biosynthesis. Enzymatic studies with purified protein(s) would facilitate the identific ...
Nonribosomal peptide synthesis in Aspergillus
... Table 1. NRP synthetase genes of A. fumigatus, with hypothetical NRP synthetase domain architecture based on in silico analysis Key: A, adenylation domain; T, thiolation domain; C, condensation domain; E, epimerase domain; TE, thioesterase domain; DH, COG3320, putative dehydrogenase domain of multif ...
... Table 1. NRP synthetase genes of A. fumigatus, with hypothetical NRP synthetase domain architecture based on in silico analysis Key: A, adenylation domain; T, thiolation domain; C, condensation domain; E, epimerase domain; TE, thioesterase domain; DH, COG3320, putative dehydrogenase domain of multif ...
Globins in Nonvertebrate Species: Dispersal by Horizontal Gene
... atmospheric 0, became available the acquisition of oxygen-binding properties was initiated, culminating in the various highly specialized functions known at present. During this evolutionary process, we suggest that (1) high oxygen affinity may have been acquired repeatedly and (2) the formation of ...
... atmospheric 0, became available the acquisition of oxygen-binding properties was initiated, culminating in the various highly specialized functions known at present. During this evolutionary process, we suggest that (1) high oxygen affinity may have been acquired repeatedly and (2) the formation of ...
Modifying the chain-length selectivity of the
... Results and discussion With increasing knowledge about the three-dimensional structures of lipases, it has become possible to explain some of the enzyme properties based on their structures. For example, the interfacial activation of lipase is revealed by the presence of a lid (or flap) structure an ...
... Results and discussion With increasing knowledge about the three-dimensional structures of lipases, it has become possible to explain some of the enzyme properties based on their structures. For example, the interfacial activation of lipase is revealed by the presence of a lid (or flap) structure an ...
Microenvironment analysis and identification of magnesium binding
... The ®rst three columns, from left, list the structures and their PDB identi®ers. The fourth column lists crystallographical Mg2+ ions as labeled in their respective structures. Site-bound ions are labeled with a single asterisk. Columns 5 through to 8 describe the FEATURE hits near the crystallograp ...
... The ®rst three columns, from left, list the structures and their PDB identi®ers. The fourth column lists crystallographical Mg2+ ions as labeled in their respective structures. Site-bound ions are labeled with a single asterisk. Columns 5 through to 8 describe the FEATURE hits near the crystallograp ...
Reverse Transcription PCR (RT-PCR): The Molecular
... The viral DNA integrates via specific, self-complimentary sequences at both ends called long terminal repeats, or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated alo ...
... The viral DNA integrates via specific, self-complimentary sequences at both ends called long terminal repeats, or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated alo ...
DOC-file of additional text
... Although physiological studies indicate that Wigglesworthia most likely provides thiamine, pantothenic acid, pyridoxine, biotin and folic acid to its host18, genomic data suggest that it has also retained the ability to synthesize some additional cofactors (e.g. protohaeme 19, 20, ubiquinone20). To ...
... Although physiological studies indicate that Wigglesworthia most likely provides thiamine, pantothenic acid, pyridoxine, biotin and folic acid to its host18, genomic data suggest that it has also retained the ability to synthesize some additional cofactors (e.g. protohaeme 19, 20, ubiquinone20). To ...
Functional characterization of LePGT1, a membrane
... cause trouble in yeast [30]. To clarify the molecular basis of the difference in prenyl-substrate specificity, several chimeric enzymes were also prepared using GPP-specific LePGT1 and a UQ biosynthetic PPT, UBIA with broad substrate specificity [23,32], and enzymatic function was characterized usin ...
... cause trouble in yeast [30]. To clarify the molecular basis of the difference in prenyl-substrate specificity, several chimeric enzymes were also prepared using GPP-specific LePGT1 and a UQ biosynthetic PPT, UBIA with broad substrate specificity [23,32], and enzymatic function was characterized usin ...
PCR - AREA
... PCR application in Agriculture and Food Sciences Gene expression changes as a result of: • Environmental and growth conditions • Food and/or treatment • Breeding schemes to improve specific characteristics • Pests Quantification of the pathogen concentration in a sample ...
... PCR application in Agriculture and Food Sciences Gene expression changes as a result of: • Environmental and growth conditions • Food and/or treatment • Breeding schemes to improve specific characteristics • Pests Quantification of the pathogen concentration in a sample ...
Gene Section TSHR (thyroid stimulating hormone receptor) Atlas of Genetics and Cytogenetics
... Miccoli P, Pacini F. Genetic analysis of the TSH receptor gene in differentiated human thyroid carcinomas. J Endocrinol ...
... Miccoli P, Pacini F. Genetic analysis of the TSH receptor gene in differentiated human thyroid carcinomas. J Endocrinol ...
Chapter 19: DNA Ligases - DNA Replication and Human
... Conditional-lethal yeast mutants with a temperature-sensitive DNA ligase accumulate non-joined DNA fragments during abortive replication at the restrictive temperature and show defects in DNA repair and recombination at the permissive temperature. Presently, it is unclear whether S. cerevisiae and S ...
... Conditional-lethal yeast mutants with a temperature-sensitive DNA ligase accumulate non-joined DNA fragments during abortive replication at the restrictive temperature and show defects in DNA repair and recombination at the permissive temperature. Presently, it is unclear whether S. cerevisiae and S ...