LEA proteins in higher plants: Structure, function, gene expression
... 3. LEA protein structure LEA proteins in higher plants are mainly composed of hydrophilic amino acids ordered in repeated sequence (e.g. Gly and Lys), forming hyper-hydrophilicness and thermal stability. Advanced structure of such protein contains nonperiodic linear and ␣-helixed structure without t ...
... 3. LEA protein structure LEA proteins in higher plants are mainly composed of hydrophilic amino acids ordered in repeated sequence (e.g. Gly and Lys), forming hyper-hydrophilicness and thermal stability. Advanced structure of such protein contains nonperiodic linear and ␣-helixed structure without t ...
video slide - Buena Park High School
... 2 Elongation. The polymerase moves downstream, unwinding the DNA and elongating the RNA transcript 5 3 . In the wake of transcription, the DNA strands re-form a double helix. ...
... 2 Elongation. The polymerase moves downstream, unwinding the DNA and elongating the RNA transcript 5 3 . In the wake of transcription, the DNA strands re-form a double helix. ...
Molecular motors: DNA takes control
... kinetically stable, Turberfield and colleagues show that they can be disassembled by using the strand-displacement mechanism to break the DNA link between motors in complexes that are situated at the centre of the aster (Fig. 1b, steps 3–4). Simply adding single-stranded DNA oligonucleotides to the ...
... kinetically stable, Turberfield and colleagues show that they can be disassembled by using the strand-displacement mechanism to break the DNA link between motors in complexes that are situated at the centre of the aster (Fig. 1b, steps 3–4). Simply adding single-stranded DNA oligonucleotides to the ...
Biotechnology Laboratory (Kallas)
... 5. Biofuels connections, RT-qPCR analysis of MEP pathway gene expression. Objective: To investigate the expression levels of selected 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes in the cyanobacterium Synechococcus sp. PCC 7002. We have a project to introduce a gene for isoprene synthase ...
... 5. Biofuels connections, RT-qPCR analysis of MEP pathway gene expression. Objective: To investigate the expression levels of selected 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes in the cyanobacterium Synechococcus sp. PCC 7002. We have a project to introduce a gene for isoprene synthase ...
Introduction
... Lactose is found in milk but not in many other environments. It would, therefore, be a waste of energy if the gene for β-galactosidase was permanently switched on. It would be more efficient if the E.coli could switch the gene on only when its enzyme was required. Two scientists, Jacob & Monod put f ...
... Lactose is found in milk but not in many other environments. It would, therefore, be a waste of energy if the gene for β-galactosidase was permanently switched on. It would be more efficient if the E.coli could switch the gene on only when its enzyme was required. Two scientists, Jacob & Monod put f ...
Regulation of transcription by Saccharomyces cerevisiae 14-3
... bmh2 mutant by the disruption of both BMH genes and the introduction of a mutated bmh2 allele [24]. We used this mutant to identify extragenic suppressor mutations bypassing the requirement of active 14-3-3 proteins. Recessive mutations in RTG3 and SIN4 resulted in growth at the restrictive temperat ...
... bmh2 mutant by the disruption of both BMH genes and the introduction of a mutated bmh2 allele [24]. We used this mutant to identify extragenic suppressor mutations bypassing the requirement of active 14-3-3 proteins. Recessive mutations in RTG3 and SIN4 resulted in growth at the restrictive temperat ...
5 min Insect DNA/RNA Preservation and Extraction Kit
... 5 min Insect DNA/RNA Preservation and Extraction Kit Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extract ...
... 5 min Insect DNA/RNA Preservation and Extraction Kit Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extract ...
The different roles of tryptophan transfer RNA in regulating trp
... of each amino acid and/or its corresponding charged tRNAs, and enable an appropriate response that would adjust their rate of synthesis. Often both an amino acid and its charged or uncharged tRNA serve as regulatory signals. This double sensing is essential because the concentration of each signal m ...
... of each amino acid and/or its corresponding charged tRNAs, and enable an appropriate response that would adjust their rate of synthesis. Often both an amino acid and its charged or uncharged tRNA serve as regulatory signals. This double sensing is essential because the concentration of each signal m ...
PITT pGLO Transformation Lab Protocol
... is interrupted by non-coding sequences introns Requires RNA splicing to convert premRNA (primary transcript; exons + introns) into mRNA (exons) ...
... is interrupted by non-coding sequences introns Requires RNA splicing to convert premRNA (primary transcript; exons + introns) into mRNA (exons) ...
GD Reagent (Genomic DNA Isolation Reagent)
... The DNA is isolated from proteins by several methods, including the digestion of proteins by the enzyme proteinase K. The proteins are removed subsequently by salting-out, organic extraction, or binding of the DNA to a solid-phase support (such as an anion-exchange column or silica technology). The ...
... The DNA is isolated from proteins by several methods, including the digestion of proteins by the enzyme proteinase K. The proteins are removed subsequently by salting-out, organic extraction, or binding of the DNA to a solid-phase support (such as an anion-exchange column or silica technology). The ...
Motif Finding
... • The hidden sequence is of length 8 • The pattern is not exactly the same in each array because random point mutations may occur in the sequences ...
... • The hidden sequence is of length 8 • The pattern is not exactly the same in each array because random point mutations may occur in the sequences ...
LECTURE #25: Translation
... into protein with help from transfer RNA (tRNA) Each carries a specific amino acid “t” shape Carries amino acids Matches codons to anticodons ...
... into protein with help from transfer RNA (tRNA) Each carries a specific amino acid “t” shape Carries amino acids Matches codons to anticodons ...
MULTIPLE CHOICE. Choose the one alternative that best completes
... 18) The amphipathic property of phospholipids can be described as ________. A) a nonpolar region facing the outside and a polar region facing the inside of a cell B) a nonpolar region that dissolves in water and a polar region that face one another C) a single polar region that is miscible in aqueou ...
... 18) The amphipathic property of phospholipids can be described as ________. A) a nonpolar region facing the outside and a polar region facing the inside of a cell B) a nonpolar region that dissolves in water and a polar region that face one another C) a single polar region that is miscible in aqueou ...
Chapter 4 (part 3)
... Allosteric Interactions • Allosteric interaction occur when specific molecules bind a protein and modulates activity • Allosteric modulators or allosteric effectors • Bind reversibly to site separate from functional binding or active site • Modulation of activity occurs through change in protein co ...
... Allosteric Interactions • Allosteric interaction occur when specific molecules bind a protein and modulates activity • Allosteric modulators or allosteric effectors • Bind reversibly to site separate from functional binding or active site • Modulation of activity occurs through change in protein co ...
Light behind the curtain: photoregulation of nuclear architecture and
... Transcriptional corepressors are commonly associated with DNAbinding proteins that contain repressive motifs, such as the plantspecific ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif (Kagale & Rozwadowski, 2011). Such multiprotein complexes bridging transcri ...
... Transcriptional corepressors are commonly associated with DNAbinding proteins that contain repressive motifs, such as the plantspecific ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif (Kagale & Rozwadowski, 2011). Such multiprotein complexes bridging transcri ...
mRNA and protein abundance for glutathione-S
... Comprehensive identification of all functional elements encoded in the human genome is a fundamental need in biomedical research. Here, we present a comparative analysis of the human, mouse, rat and dog genomes to create a systematic catalogue of common regulatory motifs in promoters and 3' untransl ...
... Comprehensive identification of all functional elements encoded in the human genome is a fundamental need in biomedical research. Here, we present a comparative analysis of the human, mouse, rat and dog genomes to create a systematic catalogue of common regulatory motifs in promoters and 3' untransl ...
Sbjct = Alu sequence
... Annealing requires lower temperatures to allow nucleic acids to associate by complementary base pairing. The primers are usually 15-25 nucleotides in length and are complementary to the ends of the region of DNA to be copied. The primer length and composition (percentage of A, T, C, and G) largely d ...
... Annealing requires lower temperatures to allow nucleic acids to associate by complementary base pairing. The primers are usually 15-25 nucleotides in length and are complementary to the ends of the region of DNA to be copied. The primer length and composition (percentage of A, T, C, and G) largely d ...
Full text PDF
... the amino terminus of CRTC2 consist of alpha helix structure, and directly bind to leucine zipper region of CREB. Since alpha helix of CRTC2 lacks basic amino acids, CRTC2 is not directly involved in the DNA binding, but promotes stronger interaction among CREB, CRTC2 and its cognate DNA (containing ...
... the amino terminus of CRTC2 consist of alpha helix structure, and directly bind to leucine zipper region of CREB. Since alpha helix of CRTC2 lacks basic amino acids, CRTC2 is not directly involved in the DNA binding, but promotes stronger interaction among CREB, CRTC2 and its cognate DNA (containing ...
RevertAid First Strand cDNA Synthesis Kit, #K1621
... Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C. First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal for second strand cDNA synthesis or linear RNA amplification. Radioactiv ...
... Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C. First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal for second strand cDNA synthesis or linear RNA amplification. Radioactiv ...
Presentation
... DNA is only macromolecule to be repaired a. Correct nucleotide has higher affinity for moving polymerase b. DNA polymerase is a “self-correcting” enzyme; has a 3’ to 5’ proofreading ...
... DNA is only macromolecule to be repaired a. Correct nucleotide has higher affinity for moving polymerase b. DNA polymerase is a “self-correcting” enzyme; has a 3’ to 5’ proofreading ...
A Discovery Laboratory Investigating Bacterial Gene Regulation
... The repressor of the lactose operon is coded for by the lac I gene. The repressor binds to the operator area, O, and blocks the attachment of the RNA polymerase to the promoter site, P. RNA polymerase must attach to the promoter site and move through the operator site if the lactose operon is to be ...
... The repressor of the lactose operon is coded for by the lac I gene. The repressor binds to the operator area, O, and blocks the attachment of the RNA polymerase to the promoter site, P. RNA polymerase must attach to the promoter site and move through the operator site if the lactose operon is to be ...
RnaUs Total Viral RNA/DNA Prep
... and centrifuge at 12,000 rpm for 2 min. The RNA is in the flow-through liquid. Store the RNA solution o at -20 C. Note: It is highly recommended that RNA quality be determined before downstream applications. The quality of RNA can be assessed by denatured agarose gel electrophoresis with the ethidiu ...
... and centrifuge at 12,000 rpm for 2 min. The RNA is in the flow-through liquid. Store the RNA solution o at -20 C. Note: It is highly recommended that RNA quality be determined before downstream applications. The quality of RNA can be assessed by denatured agarose gel electrophoresis with the ethidiu ...
2.6 Structure of DNA and RNA
... nucleotides of DNA and RNA, using circles, shape does not need to be shown, but the two pentagons and rectangles to represent phosphates, strands should be shown antiparallel. Adenine pentoses and bases. should be shown paired with thymine and guanine with cytosine, but the relative lengths of the p ...
... nucleotides of DNA and RNA, using circles, shape does not need to be shown, but the two pentagons and rectangles to represent phosphates, strands should be shown antiparallel. Adenine pentoses and bases. should be shown paired with thymine and guanine with cytosine, but the relative lengths of the p ...