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Lecture Slides
Lecture Slides

... Figure 10.00a ...
Immobilization and stretching of DNA molecules in a
Immobilization and stretching of DNA molecules in a

... A new technique can immobilize and stretch a large number of DNA molecules for single-molecule DNA analysis applications. DNA-protein interactions drive the cellular machinery for maintaining and transcribing DNA. To study the motion and kinetics of proteins along a DNA strand at the single-molecule ...
VNTR, STR and RFLP
VNTR, STR and RFLP

... – Location in a genome where a short nucleotide is organized as a tandem repeat – These can be found on many chromosomes and often show variations in length – Each variant acts as an inherited allele allowing used for identification – Useful in genetics, biology research, forensics and DNA fingerpri ...
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Snímek 1
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清华大学本科生考试试题专用纸
清华大学本科生考试试题专用纸

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Faulty ribosomes and human diseases: mistakes in “assembly line
Faulty ribosomes and human diseases: mistakes in “assembly line

... Mobile : +91 70221 29624 E-mail : [email protected] Abstract Ribosomes are molecular machineries that decode the information within mRNAs and generate all the proteins required for cellular activities. Ribosomes are essential to every living organism. The synthesis of ribosome is an intricate pro ...
Lassa Virus (LV)Real Time RT-PCR Kit User Manual For In Vitro
Lassa Virus (LV)Real Time RT-PCR Kit User Manual For In Vitro

... Molecular Grade Water is used for dilution. Dilution is not needed for qualitative real-time PCR detection. Take positive control (1×107copies/ml) as the starting high standard in the first tube. Respectively pipette 36ul Molecular Grade Water into next three tubes. Do three dilutions as the followi ...
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Facioscapulohumeral Muscular Dystrophy: The Molecular Signaling
Facioscapulohumeral Muscular Dystrophy: The Molecular Signaling

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Insights from the HuR-interacting transcriptome: ncRNAs, ubiquitin

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Pre-mRNA splicing: life at the centre of the central dogma

... still await functional characterization. Some of the additional proteins identified in these complexes have known associations with other aspects of RNA processing, including transcription and mRNA export. This is consistent with the intimate coupling of different steps in gene expression. Alternati ...
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... 1) The size of the positive data set may be increased by incorporating point mutations in non-sensitive positions 2) Negative data sets are generated by several ways i.e. a) Shuffling or randomising the positive data set. This does not destroy the the correlations between the bases completely. b) U ...
Organic Chemistry Fifth Edition
Organic Chemistry Fifth Edition

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Zygotic Genome Activation During the Maternal-to

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Lecture 2 Nucleic Acid Structure
Lecture 2 Nucleic Acid Structure

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07 NucleicAcids-06b

...  Origins of Replication  Eukaryotic chromosome -- hundreds to thousands  Replication proceeds in both directions  Replication fork – ends of each replication bubble ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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