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April 3 lecture slides
April 3 lecture slides

... Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
Regulation of Transcription
Regulation of Transcription

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Molecular genetics of gene expression

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... 1. Minisatellite DNA These are 1 to 5 kb in length consisting of repeats 15 to 100 nucleotides in length and are identified ...
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CHAPTER 19 -- EUKARYOTIC GENE EXPRESSION YOU MUST

...  Some of these mutations can be spontaneous, but mostly caused by environmental factors (mutagens) or viruses. Viruses can transfer the healthy genes to cancer causing genes by inserting their DNA into the host cell’s DNA at the gene segment.  The normal version of the genes that are responsible f ...
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Lecture #7 Date ______

... of RNA begins Terminator region: sequence that signals the end of transcription Transcription unit: stretch of DNA transcribed into an RNA molecule ...
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... four proteins (see Figures 7-52 and 7-53). The expression of eve in stripe 2 occurs only at the position where the two activators (Bicoid and Hunchback) are present and the two repressors (Giant and Krüppel) are absent. In fly embryos that lack Krüppel, for example, stripe 2 expands posteriorly. Lik ...
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... REGULATORY PROTEINS: Besides the promoter, other DNA sequences can bind regulatory proteins that interact with RNA polymerase and regulate transcription.  Some are positive regulators—activators (DNA sequence is called an enhancer); others are negative—repressors (DNA sequence is called a silencer ...
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transcription

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Eukaryotic Transcription In all species, transcription begins with the
Eukaryotic Transcription In all species, transcription begins with the

... in eukaryotes. The breakthrough came in 1996 when a number of research groups discovered that certain transcriptional coactivators are histone acetyltransferases (HATs). It has been known for some time that binding of transcriptional activators to the enhancer region, in most cases, is not sufficien ...
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3. Activator, gene-specific transcription facotr

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Brooker Chapter 15
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... In plants and animals, multicellularity and a more complex cell structure, also demand a much greater level of gene expression Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
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Allosteric Modulation of DNA by Small Molecules

... and Environmental Research, with additional support from the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program, and the National Institute of General Medical Sciences. ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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