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Extra Credit DNA Study Guide
Extra Credit DNA Study Guide

... 52. If two patterns in different lanes are identical on a gel electrophoresis, what does this mean? Circle all that apply Each band has the same amount (mass of DNA ...
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File

... ladder. The sides of the ladder consist of long chains made up of “P”s and “S”s. Each “P” stands for a ________________ and each “S” stands for a ____________. 5. The actual DNA structure is not really like a ladder, but like two coils wrapped around each other. This structure is called a double ___ ...
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Biology Assessment #3:

... 6. What is a pedigree? What can we learn from examining one? Genetic Technology 1. What is recombinant DNA and how is it formed? 2. What is gene splicing? 3. Explain the uses DNA fingerprinting. 4. How do scientists interpret a DNA fingerprint? 5. What is cloning? 6. What are mutations? How are they ...
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Worksheet Lesson 5: The discovery of DNA`s

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Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the
Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the

... act at the membrane to restrict the passage of certain molecules into the cell. are highly specialized ribonucleases that degrade mRNA soon after its synthesis. are sequence-specific DNA endonucleases. are very specific proteases that cleave peptides at only certain sequences. catalyze the addition ...
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No Slide Title

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11-GeneTech

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DNA LIBRARIES

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Timing and Development of Growth

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Nucleotide - Jackson County School District

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Figure 13-1
Figure 13-1

... c. A restriction enzyme producing a DNA fragment d. Polymerase chain reaction Figure 13-1 2. Which statements are TRUE about structures C and D? (Select more than one) a. They are sticky ends b. They are made by different restriction enzymes. c. They will attach to DNA fragments cut with the same en ...
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Chapter Objectives: Chapter 20 Biotechnology

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Data visualization in the post

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< 1 ... 614 615 616 617 618 619 620 621 622 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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