• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Vocabulary Glossary - CTAE Resource Network
Vocabulary Glossary - CTAE Resource Network

... 2. Agarose Gel: Complex sugar obtained from agar gelatin that is the most widely-used medium for gel electrophoresis procedures 3. Autoradiography: Process of making images of radiation emitted from a specimen 4. Blunt Ends: Ends of new molecules made from cleaving DNA 5. Clones: Cell, cell products ...
Slides
Slides

Structure and History of DNA 1-8
Structure and History of DNA 1-8

... “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material”. • Besides copying, DNA must do ...
Restriction Enzymes - Solon City Schools
Restriction Enzymes - Solon City Schools

... last few years, and will become more important as genetic diseases become more prevalent and agricultural area is reduced. Below are some of the areas where Recombinant DNA will have an impact: – Better Crops (drought & heat resistance) – Recombinant Vaccines (i.e. Hepatitis B) – Production of clott ...
4. The diagram below shows a segment of DNA with a total length of
4. The diagram below shows a segment of DNA with a total length of

... __ site of attachment of repressor protein __ codes for sequential protein __ serves to inactivate repressor CONTROL OF mRNA PRODUCTION & CONSEQUENCES re PROTEIN SYNTHESIS: Max. = 5 __ Inducible model: derepression (lactose example) [gene always off] = 3 points __ Repressible model: corepression (tr ...
genetic engineering
genetic engineering

... which distinguishes the human DNA from the bacterial DNA. One way to make recombinant DNA is to insert a human gene into bacterial DNA. The new combination of genes is then returned to a bacterial cell, and the bacteria can produce the human protein. ...
fix my dna text
fix my dna text

“Bill Nye: Genes” Video Worksheet
“Bill Nye: Genes” Video Worksheet

... 8. Why is the white blood cell dark on the computer screen? Because it has ...
11/01 Molecular genetic analysis and biotechnology
11/01 Molecular genetic analysis and biotechnology

... DNA via a bacteria cell • Cloning vector: a replicating DNA molecule attached with a foreign DNA fragment to be introduced into a cell – Has features that make it easier to insert DNA and select for presence of vector in cell. • Origin of replication • Antibiotic resistance gene • Cloning site ...
7.1 - DNA Structure
7.1 - DNA Structure

... proteins and held together by another histone protein. The DNA double helix has major and minor groves on the outer diameter, exposing chemical groups that can form hydrogen bonds. These groups are bonded to positively-charged proteins called histones, forming two loops around them. DNA is wound aro ...
Genetic Technology
Genetic Technology

... 1) GENETIC RECOMBINATION ...
Study Guide 3 Bio 4 C
Study Guide 3 Bio 4 C

... Morgan and white eyed vs. wild type fruit flies, mutant phenotype, sex-linked genes, examples like hemophilia, sex-influenced trait, nondisjunction, aneuploidy, translocation, Down Syndrome, Turner syndrome, Klinefelter syndrome, metafemale, XYY syndrome Ch. 20 DNA Technology genetic engineering, re ...
1. Explain how a gene directs the synthesis of an mRNA molecule
1. Explain how a gene directs the synthesis of an mRNA molecule

... T = thymine is replaced by ___ = uracil in RNA, so A in DNA pairs with ___ in mRNA. ...
Biology Chapter 11-1
Biology Chapter 11-1

... DNA Recombination - DNA fragments may be combined with bacterial DNA so they can be reentered into a bacterium. - The plasmids are removed and cut with the same restriction enzyme used to produce the DNa fragments. - The cuts leave sticky ends which are then joined together to complete the ring. DN ...
Biotechnology
Biotechnology

... • Organisms containing recombinant DNA or foreign DNA are known as transgenic ...
Micro Quiz #3R Stu F2011 - the Biology Scholars Program Wiki
Micro Quiz #3R Stu F2011 - the Biology Scholars Program Wiki

... 4. AT-rich DNA strands will denature (separate) at a(n): A. Higher temperature than GC-rich DNA B. Identical temperature as GC-rich DNA C. Similar temperature as GC-rich DNA, with minor variations D. Lower temperature than GC-rich DNA E. Temperature dependent upon whether it is from a prokaryote or ...
molecular scissors to study gene function Marta Oliveira
molecular scissors to study gene function Marta Oliveira

... interspaced by unique DNA regions that come from several bacterial viruses, which works as a DNA record of previous attacks. It was later proved that the CRISPR loci, together with many enzymes, were in fact a bacterial defense mechanism against life threatening viral attacks, preventing the virus f ...
Chapter 12 “DNA, RNA, and Protein Synthesis” Reading/Study Guide
Chapter 12 “DNA, RNA, and Protein Synthesis” Reading/Study Guide

... 2. The following scientists all contributed to solving the mystery of heredity and the double helix. Describe what each did, and if given, what experiment they used: a. Frederick Griffith ...
The Living World
The Living World

...  Vectors are plasmids or viruses that carry foreign DNA into the host cell  Vector DNA is cut with the same enzyme as the source DNA, thus allowing the joining of the two ...
Biology Benchmark Review Second Nine (SB2) Weeks 2009-2010
Biology Benchmark Review Second Nine (SB2) Weeks 2009-2010

... principle does this justify? ...
here - St Vincent College
here - St Vincent College

... Term that means the DNA has 2 strands that are twisted together (dh) There are 23 pairs of these in most human cells (c) ...
Major Events in Genetics
Major Events in Genetics

... A gene is a genetic sequence that codes for an RNA. In protein coding genes, the RNA codes for a protein. ...
After Gel Electrophoresis…
After Gel Electrophoresis…

... the gel was run the DNA segment was added to a test tube with DNA polymerase  DNA polymerase added new bases that were labeled with colors that could be read from the gel ...
UNIT 4 PART 2 APPLIED GENETICS
UNIT 4 PART 2 APPLIED GENETICS

... carry pieces of DNA from one location to another Types of Vectors: ...
Goal 3.05 Examine the Theory of Evolution by Natural
Goal 3.05 Examine the Theory of Evolution by Natural

... controversy over stem cells because scientists can get them from EMBRYOS. 11.An advantage of cloning is that it produces IDENTICAL organisms/ tissues and can be used to REPLACE diseased organs and produce consistently high yield CROPS like corn. It is a form of ASEXUAL reproduction. 12.Two advantage ...
< 1 ... 613 614 615 616 617 618 619 620 621 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report