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Study Guide for LS
Study Guide for LS

... molecules. When DNA copies itself it splits down the middle where the two bases meet. The bases on each side of the molecule can be used as a pattern for a new complementary side. ...
Biotechnology - Genetic Engineering
Biotechnology - Genetic Engineering

... 3. Restriction enzyme “cuts” the DNA into many pieces every time it recognizes its specific recognition site. 4. Place DNA sample into the gel electrophoresis apparatus. 5. One end of apparatus is negative and the other is positive (like a battery). DNA is negative. ...
DNA Structure and Function Video
DNA Structure and Function Video

... Providing you with an empty egg which could then be used  to place your iguana DNA in.  Now the NEW egg cell would  need to be placed into a reptile to help develop the egg  before being hatched.  After hatching you would get a baby  iguana that is an identical DNA match to the original iguana  you  ...
How Biologists Classify Organisms... (pg 113
How Biologists Classify Organisms... (pg 113

... Cladistics......grouping species together because they share a common ancestor. Each species still contains a part of that ancestor, but also gains new differences due to evolution and genetic mutation. Cladistics diagrams are simple stick drawings of how groups may have split from (diverged) the a ...
Chapter 12-1 Skeleton Notes
Chapter 12-1 Skeleton Notes

... Labeled the protein coat with a radioactive sulfur isotope and the DNA with a radioactive phosphorous isotope so that they may follow where each part goes after the infection Mixed solution of bacteriophage and solution of bacteria together and let virus work After a time, put mixture into a blende ...
Section 9.1 – The Structure of DNA
Section 9.1 – The Structure of DNA

... The S bacteria was virulent – or able to cause disease and was deadly! R bacteria and heat–killed S bacteria was not virulent – TRANSFORMATION! 2. Describe how Avery’s experiment supplied evidence that DNA, and not protein, is the genetic material. Avery showed that DNA–destroying enzymes but not pr ...
Deciphering the Structure of the Hereditary Material
Deciphering the Structure of the Hereditary Material

... duplicate and are shared equally between two daughter cells when the parent cell divides) was understood, as also was meiosis (the process whereby a parent cell divides twice to produce 4 sex cells (egg or sperm). The chromosomes duplicate only once during meoisis and consequently each germ cell rec ...
Human Genomics - Mrs Smith`s Biology
Human Genomics - Mrs Smith`s Biology

Given the following two evolutionary conserved eukaryotic genes A
Given the following two evolutionary conserved eukaryotic genes A

7.1 DNA Structure
7.1 DNA Structure

... • How are the two strands of nucleotides connected? • Two strands arrange themselves so that the Nbases are in the center • N-bases complementary bond with each other using hydrogen bonds ▫ Cytosine – Guanine (C-G) ▫ Adenine – Thymine (A-T) ...
Name Date ______ Period
Name Date ______ Period

... DNA Alias Graffiti DNA review questions: ...
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microbio 40 [4-20
microbio 40 [4-20

... 2. What does a lack of envelope mean in terms of transmissibility and durability? How does the virus spread?  Aids transmissibility and makes them resistant to detergents, dessication, and heat  usually by direct skin to skin contact, and even then it requires a skin break  can also be spread by ...
Molecules to Eye Color - Springfield School District
Molecules to Eye Color - Springfield School District

...  3. Single strand (not double) ...
Unit 4 Resources - Schoolwires.net
Unit 4 Resources - Schoolwires.net

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GENETIC ENGINEERING
GENETIC ENGINEERING

... – Scientists isolated the DNA sequence that regulates the production of insulin – The DNA segment is spliced into the DNA of the E.coli bacteria – The bacteria carrying the DNA for insulin production reproduces and passes the capability along to the next generation ...
DNA Fingerprinting
DNA Fingerprinting

... 1. Enzymes cut DNA samples 2. The cut DNA samples are placed in a gel 3. An electric voltage moves the DNA fragments across the gel 4. The smaller pieces of DNA travel the fastest 5. A trail is left for each sample. 6. The trail can be matched to determine if someone is guilty of a crime. Gel Electr ...
DNA and RNA Notes
DNA and RNA Notes

... DNA and RNA Notes Discovery of DNA  _____________ - pneumonia causing bacteria and mice. (Determined…)  _____________ - process of one bacteria changing its DNA from the addition of another.  Avery- DNA is the nucleic acid that ___________ and __________ genetic information from one generation to ...
Gene Cloning and Karyotyping
Gene Cloning and Karyotyping

... – Every time this cell reproduces, the recombinant plasmid is replicated as well and passed on to its descendents. – Under suitable conditions, the bacterial clone will make the protein encoded by the foreign gene. ...
CS 262—Lecture 1 Notes • 4-‐5 HWs, 3 late days • (Optional
CS 262—Lecture 1 Notes • 4-‐5 HWs, 3 late days • (Optional

... • DNA  in  nucleus  and  each  cell  has  copies  of  DNA   o Although  we  have  trillions  of  cells,  an  average  somatic  cell  only  has   30-­‐60  differences  from  the  “pure”,  original  genome   • DNA  packed  into  chromosom ...
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... a) allows DNA from sources to be joined b) nicks are linked by forming a c. creates recombinant molecules B. Host/Vector 1. produces a large amount of in cells a. vector carries the 2. Most common a. plasmids – small chromosomes 1) clones pieces of DNA a) replicated in b) selectable marker – only ce ...
Themes in the Development of DNA Science
Themes in the Development of DNA Science

... the genetic code. RNA viruses store genetic info as RNA Genes are not immutably fixed on the chromosomes. Transposable genetic elements move around from one chromosome to another and may act as molecular switches to regulate gene expression. DNA sequence and protein sequence are not entirely colinea ...
Ch. 4 Nucleic Acids Define
Ch. 4 Nucleic Acids Define

... 1. What is the name of the structure shown below? Define its 3 components. ...
Green Genes: DNA Curriculum
Green Genes: DNA Curriculum

Does your DNA define you Qu
Does your DNA define you Qu

... nucleotide cytosine and gets incorporated into DNA. When it is present in DNA, it ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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