• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Impact of Computer Technology in Molecular Biology and Genetics
Impact of Computer Technology in Molecular Biology and Genetics

... Previous versions of BLAST did not account for ...
eprint_12_13279_954
eprint_12_13279_954

Frequently Asked Questions.
Frequently Asked Questions.

... in DNA mean, for instance, that a certain type of brain cell can be more (or less) active than another. Thus DNA plays a role in our behaviour. ...
Cells are exposed to DNA damaging agents that can affect their
Cells are exposed to DNA damaging agents that can affect their

... These are important steps to solve because most of the molecules of interest in biology do not have any symmetry, especially those of the so-called “molecular machines” where a collection of proteins interact to work in such various processes as DNA repair or RNA splicing. Also, some important molec ...
slides
slides

... • Bacterial genomes are streamlined, genes are closely packed with relatively little spacer DNA and few introns • Mammalian genome contains enormous amounts of nongene DNA including introns, spacer DNA between genes and various types of repetitive DNA sequences with similarities to transposons. Also ...
Molecular Genetics
Molecular Genetics

... Eukaryote Gene Regulation  Controlling transcription  Transcription factors ensure that a gene is used at the right time and that proteins are made in the right amounts ...
2421 _Ch8.ppt
2421 _Ch8.ppt

... The process repeats so that one amino acid is added at a time to the growing polypeptide (which is always anchored to a tRNA bound within the ribosome) The polypeptide continues to grow until the ribosome reaches a stop codon At the stop codon, the polypeptide chain is released from the last tRNA a ...
Study Guide: Meiosis and Genetics
Study Guide: Meiosis and Genetics

... 6.2.1 Draw and label a diagram of the heart showing the four chambers, blood vessels, valves and the route of blood through the heart. ...
Document
Document

... • LINE and SINE repeats. A LINE (long interspersed nuclear element) encodes a reverse transcriptase (RT) and perhaps other proteins. Mammalian genomes contain an old LINE family, called LINE2, which apparently stopped transposing before the mammalian radiation, and a younger family, called L1 or LI ...
Powerpoint slides - School of Engineering and Applied Science
Powerpoint slides - School of Engineering and Applied Science

... • You need to know what you are looking for, e.g. the DNA sequence for a particular gene (the target). • Sample, primers, nucleotides to build new DNA strands, and Taq polymerase mixed together. • Mixture is subjected to cycles of heating, cooling, reheating, on the order of a few minutes. ...
BIMM 101 Recombinant DNA Techniques Credit by Exam Student
BIMM 101 Recombinant DNA Techniques Credit by Exam Student

... a) Outline the general strategy used to isolate DNA from organisms b) Describe the characteristics of a gram negative bacteria c) Explain how chromosomal DNA is isolated from bacteria using phenol-chloroform method d) Explain how plasmid DNA is isolated from bacteria using the alkaline lysis method ...
applied-genetics-questions with answers (1)
applied-genetics-questions with answers (1)

Lab 8
Lab 8

... Most cells in our body contain DNA, the molecule that makes up the genes that control our physical characteristics. Half your DNA came from your mother, and half from your father. Your DNA is what determines your eye color, hair color, height, blood type, and all other physical properties of your bo ...
Sunflower DNA extraction for RFLP and PCR
Sunflower DNA extraction for RFLP and PCR

... polysaccharides and tannins. SDS-KAc method and common CTAB method (precipitate DNA directly from DNA-CTAB buffer using isopropanol) can not produce highly purified DNA because DNA co-precipitates with above secondary metabolites. This modified CTAB method is relatively simple, and can provide high ...
Biology Pre-Learning Check
Biology Pre-Learning Check

... Bacteria can reproduce in 4 different ways. Describe each of these: ...
What is Biology? - sunysuffolk.edu
What is Biology? - sunysuffolk.edu

... • Evolution is a gradual change that occurs over a long period of time • Evolution explains the diversity and adaptations of life • Evolution is the change in genetic material of a population of organisms from one generation to the next ...
What is Biology?
What is Biology?

... • Evolution is a gradual change that occurs over a long period of time • Evolution explains the diversity and adaptations of life • Evolution is the change in genetic material of a population of organisms from one generation to the next ...
Chapter 4 Molecular Cloning Methods
Chapter 4 Molecular Cloning Methods

... variety of unique restriction sites into which one can introduce foreign ...
VNTR, STR and RFLP
VNTR, STR and RFLP

... – Location in a genome where a short nucleotide is organized as a tandem repeat – These can be found on many chromosomes and often show variations in length – Each variant acts as an inherited allele allowing used for identification – Useful in genetics, biology research, forensics and DNA fingerpri ...
Replication, Transcription, and Translation
Replication, Transcription, and Translation

... Helicase unzips/unwinds the DNA molecule DNA Polymerase brings in new nucleotides Ligase zips the new DNA back together Why is DNA Replication important?  The important idea is that an exact duplication of the DNA message is required, so that each new cell in the body has the same set of genetic in ...
The Genetic Material
The Genetic Material

... (Sutton and Boveri 1902) ƒ Chromosomes are in pairs and genes, or their alleles, are located on chromosomes ƒ Homologous chromosomes separate during meiosis so that alleles are segregated ƒ Meiotic products have one of each homologous chromosome but not both ƒ Fertilization restores the pairs of chr ...
Lecture 15: Bacterial Genetics I
Lecture 15: Bacterial Genetics I

Nucleic Acids - cpprashanths Chemistry
Nucleic Acids - cpprashanths Chemistry

... ATP is a single nucleotide high energy molecule produced by cellular respiration • transfers energy within cells ...
T4 DNA Polymerase
T4 DNA Polymerase

... recessed 3´-termini (7). On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3´-ends along the length of the template. Exonuclease III from E. coli can be used to create partially single-stranded dsDNA for subsequent polymerization reactions (8). Mo ...
Brooker Chapter 9
Brooker Chapter 9

... (Sutton and Boveri 1902)  Chromosomes are in pairs and genes, or their alleles, are located on chromosomes ...
< 1 ... 414 415 416 417 418 419 420 421 422 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report