2. You perform a Southern blot in which your probe should hybridize
... 2. You touched the membrane with ungloved hands. NOTE: This would be because probe stuck to oils or other debris from your hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available fo ...
... 2. You touched the membrane with ungloved hands. NOTE: This would be because probe stuck to oils or other debris from your hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available fo ...
A-DNA
... A gene in relation to the double helix structure of DNA and to a chromosome (right). Introns are regions often found in eukaryote genes that are removed in the splicing process (after the DNA is transcribed into RNA): only the exons encode the protein. This diagram labels a region of only 40 or so b ...
... A gene in relation to the double helix structure of DNA and to a chromosome (right). Introns are regions often found in eukaryote genes that are removed in the splicing process (after the DNA is transcribed into RNA): only the exons encode the protein. This diagram labels a region of only 40 or so b ...
Chapter 14: Human Heredity
... 1. Summarize the main steps in cloning. 2. Explain the production, use, benefits and controversy of genetically modified foods. 3. Explain how microarrays show important connections between cell biology, DNA, genes, gene expression, transcription, translation, cancer, proteins, and bioethics. 4. Des ...
... 1. Summarize the main steps in cloning. 2. Explain the production, use, benefits and controversy of genetically modified foods. 3. Explain how microarrays show important connections between cell biology, DNA, genes, gene expression, transcription, translation, cancer, proteins, and bioethics. 4. Des ...
DNA Structure and DNA Replication Practice Problems
... DNA Replication. Use the hypothetical representation of a double stranded DNA molecule, below, to complete the following tasks. a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Label the 5’ and 3’ ends of each strand. c. Use dashed lines ...
... DNA Replication. Use the hypothetical representation of a double stranded DNA molecule, below, to complete the following tasks. a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Label the 5’ and 3’ ends of each strand. c. Use dashed lines ...
BIO101 Objectives Unit3 Blinderman Mercer County Community
... 5. Contrast mitosis and cytokinesis 6. Examine in detail interphase and mitosis phases of the cell cycle 7. Compare the G1, S, G2, and Go phases of interphase in terms of cellular events 8. Follow formation of sister chromatids during interphase and mitosis (P,M,A,T) 9. Describe the formation and ro ...
... 5. Contrast mitosis and cytokinesis 6. Examine in detail interphase and mitosis phases of the cell cycle 7. Compare the G1, S, G2, and Go phases of interphase in terms of cellular events 8. Follow formation of sister chromatids during interphase and mitosis (P,M,A,T) 9. Describe the formation and ro ...
Do-It-Yourself Strawberry DNA
... isolation is one of the most basic and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA ...
... isolation is one of the most basic and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA ...
DNA and Genealogy
... the Cambridge Reference Sequence for mtDNA, used for convenience to compare with all human mtDNA sequences, such that any sequence can be expressed concisely as a relatively short list of difference from the reference ...
... the Cambridge Reference Sequence for mtDNA, used for convenience to compare with all human mtDNA sequences, such that any sequence can be expressed concisely as a relatively short list of difference from the reference ...
Whole genome shotgun sequencing
... (b) Sequence of mutant allele Hybridize each oligo (separately) to Southern blot of DNA. Use conditions that allow only oligonucleotides that are 100% complementary to DNA on blot to hybridize. If only normal oligo hybridizes---homozygous normal allele If only mutant oligo hybridizes --- homozygous ...
... (b) Sequence of mutant allele Hybridize each oligo (separately) to Southern blot of DNA. Use conditions that allow only oligonucleotides that are 100% complementary to DNA on blot to hybridize. If only normal oligo hybridizes---homozygous normal allele If only mutant oligo hybridizes --- homozygous ...
Forensics of DNA
... individual cannot be __________________ as the ______________ of the DNA by any of the tests, a point is reached at which the tests have excluded virtually the world's _____________________ and the unique identification of that individual as the source of the DNA has been ________________. 4. Possib ...
... individual cannot be __________________ as the ______________ of the DNA by any of the tests, a point is reached at which the tests have excluded virtually the world's _____________________ and the unique identification of that individual as the source of the DNA has been ________________. 4. Possib ...
الشريحة 1
... build a resistance against antibiotics or poisons. • Col-plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. • Degradative plasmids, which enable the digestion of unusual substances, e.g., salicylic acid. • Virulence plasmids, which turn the bacterium int ...
... build a resistance against antibiotics or poisons. • Col-plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. • Degradative plasmids, which enable the digestion of unusual substances, e.g., salicylic acid. • Virulence plasmids, which turn the bacterium int ...
DNA repair DNA as genetic information
... • DNA is more stable than RNA and double‐stranded making it a perfect molecule for storage of information • Cellular repair mechanisms prevent accumulation of unwanted mutations by repairing 999/1000 mutations • DNA repair is dependent on double‐stranded DNA • RNA and proteins are also damaged but ...
... • DNA is more stable than RNA and double‐stranded making it a perfect molecule for storage of information • Cellular repair mechanisms prevent accumulation of unwanted mutations by repairing 999/1000 mutations • DNA repair is dependent on double‐stranded DNA • RNA and proteins are also damaged but ...
Gene Technology
... which attaches to the DNA next to the promoter sequence preventing the attachment of RNA polymerase. However in the presence of lactose, lactose molecules attach to the repressor changing its configuration so that it no longer attaches to the DNA. This allows RNA polymerase to attach & express the g ...
... which attaches to the DNA next to the promoter sequence preventing the attachment of RNA polymerase. However in the presence of lactose, lactose molecules attach to the repressor changing its configuration so that it no longer attaches to the DNA. This allows RNA polymerase to attach & express the g ...
Science - Mansfield ISD
... (College and Career Readiness Standards) 6B Recognize that components that make up the genetic code are common to all organisms. (EOC Supporting Standard) ...
... (College and Career Readiness Standards) 6B Recognize that components that make up the genetic code are common to all organisms. (EOC Supporting Standard) ...
Genetics and Heredity
... only one allele (B) for the baldness trait to be expressed, while a bald woman must be homozygous for the trait (BB). ...
... only one allele (B) for the baldness trait to be expressed, while a bald woman must be homozygous for the trait (BB). ...
Nucleic Acids and Genetics - Travis Science TAKS Practice
... Label DNA diagram – A-F see web site ...
... Label DNA diagram – A-F see web site ...
Chapter 12 Genetic Engineering and the Molecules of Life
... against viruses and bacteria by inserting DNA encoding for a viral or bacterial protein into a vector. The protein is expressed and then isolated and purified. This protein is then used to in a vaccine to stimulate the immune system to produce antibodies against it. A subsequent infection by the vir ...
... against viruses and bacteria by inserting DNA encoding for a viral or bacterial protein into a vector. The protein is expressed and then isolated and purified. This protein is then used to in a vaccine to stimulate the immune system to produce antibodies against it. A subsequent infection by the vir ...
DNA Mutation and Repair
... A base analogue is a substance other than a standard nucleic acid base that can be incorporated into a DNA molecule by the normal process of polymerization. Such a substance must be able to pair with the base on the complementary strand being copies, or the 3'->5' editing function will remove it. Fo ...
... A base analogue is a substance other than a standard nucleic acid base that can be incorporated into a DNA molecule by the normal process of polymerization. Such a substance must be able to pair with the base on the complementary strand being copies, or the 3'->5' editing function will remove it. Fo ...
Amsterdam 2004
... Accession: GO:0005665 Ontology: cellular_component Synonyms: related: DNA-directed RNA polymerase II activity Definition: – RNA polymerase II, one of three eukaryotic nuclear RNA polymerases, is a multisubunit complex; it produces mRNAs, snoRNAs, and some of the snRNAs. Two large subunits comprise t ...
... Accession: GO:0005665 Ontology: cellular_component Synonyms: related: DNA-directed RNA polymerase II activity Definition: – RNA polymerase II, one of three eukaryotic nuclear RNA polymerases, is a multisubunit complex; it produces mRNAs, snoRNAs, and some of the snRNAs. Two large subunits comprise t ...
DNA upgrade supplement WITH PICS
... units. Unfortunately, knowing the components of DNA did not solve the mystery of whether the DNA or protein component of chromosomes functioned as genes. The next steps in identifying the hereditary molecule involved determining exactly which component carried genetic information. Scene 16: In 1928, ...
... units. Unfortunately, knowing the components of DNA did not solve the mystery of whether the DNA or protein component of chromosomes functioned as genes. The next steps in identifying the hereditary molecule involved determining exactly which component carried genetic information. Scene 16: In 1928, ...
How We Know What Happened When
... several hundred to several thousand mitochondria per cell. This means that the mitochondrial (mt) genome is highly amplified. While each cell contains only two copies of a given nuclear gene (one on each of the paired chromosomes), there are thousands of copies of a given mt gene per cell. Because o ...
... several hundred to several thousand mitochondria per cell. This means that the mitochondrial (mt) genome is highly amplified. While each cell contains only two copies of a given nuclear gene (one on each of the paired chromosomes), there are thousands of copies of a given mt gene per cell. Because o ...
DNA/RNA.lecture
... b. peptide bond formation c. translocation d. repeat 3. chain termination stop codon release factor E. polyribosomes/polysomes F. proteins are folding into their final shape G. post-translational modifications 1. modified 2. signal sequences of protein a. proteins for the RER 1) signal peptide/seque ...
... b. peptide bond formation c. translocation d. repeat 3. chain termination stop codon release factor E. polyribosomes/polysomes F. proteins are folding into their final shape G. post-translational modifications 1. modified 2. signal sequences of protein a. proteins for the RER 1) signal peptide/seque ...
From Gene to Protein
... • Genes have instructions for making proteins, but genes do not make proteins directly • Transcription is the synthesis of RNA under the direction of DNA. DNA provides the template. Get an accurate copy; mRNA • Translation is the actual synthesis of a polypeptide, at the ribosome, under the directio ...
... • Genes have instructions for making proteins, but genes do not make proteins directly • Transcription is the synthesis of RNA under the direction of DNA. DNA provides the template. Get an accurate copy; mRNA • Translation is the actual synthesis of a polypeptide, at the ribosome, under the directio ...
13-2 Manipulating DNA
... Making Copies In order to study genes, biologists often need to make many copies of a particular gene. Like a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is su ...
... Making Copies In order to study genes, biologists often need to make many copies of a particular gene. Like a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is su ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.