November 2010 Prof Angela van Daal Forensic DNA
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
Viruses (4)
... That DNA is then transcribed to mRNA using the cell’s machinery. RNA errors are great – mutation level is high Animal immune systems often can’t keep up. Two strains of viral genomes can recombine to form another virus. SIV to HIV ...
... That DNA is then transcribed to mRNA using the cell’s machinery. RNA errors are great – mutation level is high Animal immune systems often can’t keep up. Two strains of viral genomes can recombine to form another virus. SIV to HIV ...
Protein Synthesis
... • Occurs in the Nucleus • RNA polymerase binds to a spot on DNA that codes for a specific gene. RNA polymerase is an enzyme that builds a strand of RNA from DNA. • RNA polymerase unwinds & opens the DNA exposing the nucleotides. • RNA polymerase reads the DNA and links the complementary nucleotides ...
... • Occurs in the Nucleus • RNA polymerase binds to a spot on DNA that codes for a specific gene. RNA polymerase is an enzyme that builds a strand of RNA from DNA. • RNA polymerase unwinds & opens the DNA exposing the nucleotides. • RNA polymerase reads the DNA and links the complementary nucleotides ...
Prodigiosin Production in E. Coli
... have been over diluted (we had our samples suspended in 1ml of solution, when Dr. Schwekendiek noted 100µl was the usual dilution) - To rectify this, we concentrated our DNA in a Speed Vacuum Concentrator overnight - After running our samples through the Speed Vacuum Concentrator, we ran them throug ...
... have been over diluted (we had our samples suspended in 1ml of solution, when Dr. Schwekendiek noted 100µl was the usual dilution) - To rectify this, we concentrated our DNA in a Speed Vacuum Concentrator overnight - After running our samples through the Speed Vacuum Concentrator, we ran them throug ...
Ever since the days of Rene Descartes, the French philosopher
... resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The normal E. coli cells do not carry resistance against any of these antibiotics. (iii) Cloning sites: In order to link the alien DNA, the vector nee ...
... resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The normal E. coli cells do not carry resistance against any of these antibiotics. (iii) Cloning sites: In order to link the alien DNA, the vector nee ...
Chapter 4 - Fullfrontalanatomy.com
... Concept Check An organism’s genetic information is stored within the sequence of ___________. This information is transcribed into a sequence of ____________, which are then translated into a sequence of ___________. a. DNA bases, amino acids, RNA ...
... Concept Check An organism’s genetic information is stored within the sequence of ___________. This information is transcribed into a sequence of ____________, which are then translated into a sequence of ___________. a. DNA bases, amino acids, RNA ...
Difference between RNA and DNA
... 3. This can be VERY serious or it may make no difference at all! Why could it be serious? 4. What are some things that can cause a mutation in the DNA? Genetic Research. 1. Cloning: The process of making _____________ offspring from the cells of an organism. This is used in: 2. Genetic Engineering: ...
... 3. This can be VERY serious or it may make no difference at all! Why could it be serious? 4. What are some things that can cause a mutation in the DNA? Genetic Research. 1. Cloning: The process of making _____________ offspring from the cells of an organism. This is used in: 2. Genetic Engineering: ...
Agrobacterium tumefaciens
... Yeast genes can functionally be expressed when fused to the green fluorescent protein (GFP) thus allowing to localize gene products in the living cell by fluorescence microscopy The yeast system has also proven an invaluable tool to clone and to maintain large segments of foreign DNA in yeast artifi ...
... Yeast genes can functionally be expressed when fused to the green fluorescent protein (GFP) thus allowing to localize gene products in the living cell by fluorescence microscopy The yeast system has also proven an invaluable tool to clone and to maintain large segments of foreign DNA in yeast artifi ...
general biology final exam review guide
... Be able to solve monohybrid, dihybrid, incomplete dominance, sex-linked and blood type genetic problems ...
... Be able to solve monohybrid, dihybrid, incomplete dominance, sex-linked and blood type genetic problems ...
dna
... • When a cell reproduces, a complete copy of the DNA must pass from one generation to the next. • Watson and Crick’s model for DNA suggested that DNA replicates by a template mechanism. ...
... • When a cell reproduces, a complete copy of the DNA must pass from one generation to the next. • Watson and Crick’s model for DNA suggested that DNA replicates by a template mechanism. ...
Chapter 8 - Laboratory Animal Boards Study Group
... 21. T/F cytogenetic markers involve various chromosomal staining technologies which identify polymorphisms. 22. T/F allelic variants within a blood group system result from variations in amino acid sequences of cell surface proteins. 23. Which of the following is true: a. ape blood can be typed for ...
... 21. T/F cytogenetic markers involve various chromosomal staining technologies which identify polymorphisms. 22. T/F allelic variants within a blood group system result from variations in amino acid sequences of cell surface proteins. 23. Which of the following is true: a. ape blood can be typed for ...
Document
... 4. Crime Scene Investigators search in areas of the genome that are unique from individual to individual and are “anonymous” (control no known trait or function) The areas examined are Short Tandem Repeats or STR’s ...
... 4. Crime Scene Investigators search in areas of the genome that are unique from individual to individual and are “anonymous” (control no known trait or function) The areas examined are Short Tandem Repeats or STR’s ...
How Genes and Genomes Evolve
... • Most cell types can be cultured but only cells that express telomerase can be immortalized • DNA can be cut reliably and in a repeatable manner using restriction enzymes – Be aware of the details of restriction endonucleases ...
... • Most cell types can be cultured but only cells that express telomerase can be immortalized • DNA can be cut reliably and in a repeatable manner using restriction enzymes – Be aware of the details of restriction endonucleases ...
ppt
... •More widely divergent groups, rRNA or protein sequences •Comparing bacteria with eukaryotes, use conserved protein sequences •Proteins more conserved to due degeneracy of codons •Different evolutionary rates between nucleotides in codons •DNA sequences biased because of codon preferences •Two rando ...
... •More widely divergent groups, rRNA or protein sequences •Comparing bacteria with eukaryotes, use conserved protein sequences •Proteins more conserved to due degeneracy of codons •Different evolutionary rates between nucleotides in codons •DNA sequences biased because of codon preferences •Two rando ...
Mitosis
... 32. When genetic equilibrium is reached, the allele frequencies remain ____________________________. The _______________________ occurs when genetic equilibrium is disrupted. 33. When unrelated species begin to look alike because they occupy the same environment, this is _____________________ evolut ...
... 32. When genetic equilibrium is reached, the allele frequencies remain ____________________________. The _______________________ occurs when genetic equilibrium is disrupted. 33. When unrelated species begin to look alike because they occupy the same environment, this is _____________________ evolut ...
Chapter 20 DNA Technology and Genomics
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
Mitosis
... 32. When genetic equilibrium is reached, the allele frequencies remain ____________________________. The _______________________ occurs when genetic equilibrium is disrupted. 33. When unrelated species begin to look alike because they occupy the same environment, this is _____________________ evolut ...
... 32. When genetic equilibrium is reached, the allele frequencies remain ____________________________. The _______________________ occurs when genetic equilibrium is disrupted. 33. When unrelated species begin to look alike because they occupy the same environment, this is _____________________ evolut ...
DNA-Mediated Transformation
... pneumococcus loses its capsule after some invitro culture. Staphylococcus aureus is not able to produce golden pigment in absence of oxygen. Salmonella species loses flagella when exposing phenol on culture. Adding calcium to Bacillus anthracis culture stops converting bacteria to spore form. ...
... pneumococcus loses its capsule after some invitro culture. Staphylococcus aureus is not able to produce golden pigment in absence of oxygen. Salmonella species loses flagella when exposing phenol on culture. Adding calcium to Bacillus anthracis culture stops converting bacteria to spore form. ...
Bulletin - Sigma
... and elongation will be arrested. Long and Accurate (LA) PCR combines a highly processive thermostable polymerase with a second thermostable polymerase that exhibits a 3’→5’ exonucleolytic activity. This blend increases the length of amplification products by using the proofreading polymerase to repa ...
... and elongation will be arrested. Long and Accurate (LA) PCR combines a highly processive thermostable polymerase with a second thermostable polymerase that exhibits a 3’→5’ exonucleolytic activity. This blend increases the length of amplification products by using the proofreading polymerase to repa ...
Chapter 10 Topic: RNA transcription Main concepts: •Beadle and
... • If something is wrong with either the DNA sequence (the gene) or the mRNA transcription, there will be errors in the amino acid chain. Some errors will be of little consequence. Some will change the nature of the protein and cause it to misfunction. • Most mutations are the result of base substitu ...
... • If something is wrong with either the DNA sequence (the gene) or the mRNA transcription, there will be errors in the amino acid chain. Some errors will be of little consequence. Some will change the nature of the protein and cause it to misfunction. • Most mutations are the result of base substitu ...
TrueAllele Makes the Difference
... genotyping for all mixture interpretation. TrueAllele has set the standard for computerautomated interpretation for more than 15 years. With users in the United States and internationally, the TrueAllele expert team can help ensure that your transition from current interpretation methods is easy and ...
... genotyping for all mixture interpretation. TrueAllele has set the standard for computerautomated interpretation for more than 15 years. With users in the United States and internationally, the TrueAllele expert team can help ensure that your transition from current interpretation methods is easy and ...
The ATM repair pathway inhibits RNA polymerase I transcription in
... code for each 1 amino acid in a protein. ...
... code for each 1 amino acid in a protein. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.