• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Biochemistry Lecture 20
Biochemistry Lecture 20

... • 2 DNA strands/helix • Nucleotide seq of 1 strand automatically specifies seq of complementary strand – Base pairing rule: A w/ T and G w/ C ONLY in healthy helix – Each strand can serve as template for its partner ...
Chromatin: a multi-scale jigsaw puzzle
Chromatin: a multi-scale jigsaw puzzle

... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
Viruses
Viruses

... That DNA is then transcribed to mRNA using the cell’s machinery. RNA errors are great – mutation level is high Animal immune systems often can’t keep up. Two strains of viral genomes can recombine to form another virus. SIV to HIV ...
dna tech 2014 - Valhalla High School
dna tech 2014 - Valhalla High School

... Transgenic Organisms –Transgenic Animals • Transgenic animals have been used to study genes and to improve the food supply. • Mice have been produced with human genes that make their immune systems act similarly to those of humans. This allows scientists to study the effects of diseases on the huma ...
dna tech 2014 - Valhalla High School
dna tech 2014 - Valhalla High School

... Transgenic Organisms –Transgenic Animals • Transgenic animals have been used to study genes and to improve the food supply. • Mice have been produced with human genes that make their immune systems act similarly to those of humans. This allows scientists to study the effects of diseases on the huma ...
Bacteria - Rochester Community Schools
Bacteria - Rochester Community Schools

... • Horizontal gene transfer (HGT) refers to the transfer of genes between organisms in a manner other than traditional reproduction. Also termed lateral gene transfer, it contrasts with vertical transfer, the transmission of genes from the parental generation to offspring via sexual or asexual repro ...
RNA Synthesis (Transcription)
RNA Synthesis (Transcription)

... RNA Synthesis (Transcription) Introduction  First step in gene expression  RNA is involved in the transfer of genetic information stored in DNA to ...
wattsmisc03 - Centre for Genomic Research
wattsmisc03 - Centre for Genomic Research

... things can happen and odd strings of sequence can start to grow in length. Sometimes, the DNA copying machinery stutters and duplicates a particular sequence of nucleotides. Once this has happened it is more likely to happen again in the same place, so patches of repeating sequence can expand over m ...
Review on DNA Computing based Authentication Techniques
Review on DNA Computing based Authentication Techniques

... serious computation and for ultra-compact data storage. DNA cryptography is a whole new created cryptographic discipline turn out with the examination of DNA processing [1][2], wherein DNA is used as facts service and the cuttingedge biological automation is used as processing tool. The large parall ...
acta 20 - Pontifical Academy of Sciences
acta 20 - Pontifical Academy of Sciences

... Before giving a more detailed explanation for these molecular mechanisms and strategies, it might be helpful for a better understanding to outline the main elements of the theory of molecular evolution (Arber, 2003, 2007) which is also called here Molecular Darwinism. We will see that a number of no ...
10c
10c

... Introduction  Viruses infect organisms by – binding to receptors on a host’s target cell, – injecting viral genetic material into the cell, and – hijacking the cell’s own molecules and organelles to produce new copies of the virus. ...
Biology GENETICS Practice Test with Answer Key
Biology GENETICS Practice Test with Answer Key

... 33. As each section of the genetic code on DNA is transcribed to mRNA, the two strands of DNA rejoin. Then the mRNA moves into the cytoplasm through a pore in the nuclear membrane. Ribosomes attach to the mRNA, in the cytoplasm, to carry out the formation of a protein. What is this process called? ...
Course Competencies Template
Course Competencies Template

... Course Description (limit to 50 words or less, must correspond with course description on Form 102): This course is an introduction to the mechanisms of transmission of hereditary information. Students will learn the classical Mendelian principles of heredity, deviation of Mendelian principles, gene ...
60Ch14DNAhistory2008..
60Ch14DNAhistory2008..

... associated phenotype with specific chromosome  white-eyed male had specific ...
Genetics exam 4
Genetics exam 4

... The simultaneous occurrence of mRNA synthesis and protein synthesis in prokaryotes is called _________________________________________. A wild type bacterial strain capable of growth in a defined minimal medium containing only a carbon source and inorganic compounds is called a(n) __________________ ...
Transformation of the bacterium E. coli using a gene for green
Transformation of the bacterium E. coli using a gene for green

... profound changes. Examples are Agrobacterium tumefaciens (for plants) and HIV (for Humans). The bacterium you will be transforming, E.coli, lives in the human gut and is a relatively simple and well understood organism. Its genetic material consists mostly of one large circle of DNA 3-5 million base ...
DNA! - Chapter 10
DNA! - Chapter 10

... What holds our genetic coding? • Chromosomes ✓ Strands of DNA that contain all of the genes an organism needs to survive and reproduce ...
Forensic Science
Forensic Science

... •When the film is processed, bands appear where radioactive probes stuck to fragments on the nylon sheet. •A typical DNA fragment pattern will show two bands (one RFLP from each chromosome). •When comparing the DNA fragment patterns of two or more specimens, one merely looks for a match between the ...
Document
Document

... One allele masked another, one allele was dominant over the other in the F1 generation. • 2. Principle of Segregation: When gametes are formed, the pairs of hereditary factors (genes) become separated, so that each sex cell (egg/sperm) receives only one kind of gene. ...
DNA/RNA Positive Controls - Bio
DNA/RNA Positive Controls - Bio

... As the number and scope of disease-producing pathogens and their genetic variants that cause human disease have continued to increase, there has been a commensurate and rapid increase in the use of nucleic acid based tests for routine clinical diagnosis. Due to the complex nature of nucleic acids, t ...
Glossary - Crop Genebank Knowledge Base
Glossary - Crop Genebank Knowledge Base

... Recombination: Also known as crossing over. The production of a DNA molecule with segments derived from more than one parental DNA molecule. In eukaryotes, this is achieved by the reciprocal exchange of DNA between non-sister chromatids within a homologous pair of chromosomes during prophase of the ...
Everything you wanted to know about ENCODE
Everything you wanted to know about ENCODE

Problem Set 1 Questions
Problem Set 1 Questions

... 12. (a) In how many cases in the genetic code would you fail to know the amino acid specified by a codon if you know only the first two nucleotides of the codon? (b). In how many cases would you fail to know the first two nucleotides of the codon if you know which amino acid is specified by it? 13. ...
Chapter 17 Recombinant DNA and Biotechnology
Chapter 17 Recombinant DNA and Biotechnology

B2-Topic-1-notes - Greenacre Academy Trust
B2-Topic-1-notes - Greenacre Academy Trust

... E.g2 beta-carotene in golden rice to reduce vitamin A deficiency in humans: Lack of vitamin A: o can cause the immune system to stop working properlycan lead to death o can cause blindness Beta-carotene is needed by humans to make vitamin A Two extra genes can be inserted into normal rice plants to ...
< 1 ... 241 242 243 244 245 246 247 248 249 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report