• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Unit 11.1 Gene Transfer
Unit 11.1 Gene Transfer

... What are the basic functions of DNA and RNA? A. DNA - deoxyribonucleic acid is a very complex substance composed of large molecules that are capable of being put together in an almost unlimited number of ways. B. DNA - make up chromosomes. Chromosomes are contributed by each parent and determine how ...
Chapter 3: Reproduction and Heredity
Chapter 3: Reproduction and Heredity

... In humans and many other animals, chromosomes are classified into two groups. Two of the chromosomes are called sex chromosomes. They are abbreviated X and Y. Males are XY (one of each type of chromosomes in their cells). Females are XX (no Y’s). Fraternal twins receive different sets of genes from ...
Nervous System
Nervous System

... Making a polypeptide (chain of amino acids) using an mRNA template.  mRNA = copy of gene  Every 3 letters on mRNA = codon = codes for a specific amino acid (a.a.). e.g.: the codon AUG codes for methionine (“met”)  Amino acids will be bonded together in a specific sequence. Correct a.a. sequence ...
The Recombinant DNA Controversy: A Contemporary
The Recombinant DNA Controversy: A Contemporary

... DNA technology is about cloning organisms: plants, animals, and even people. It isn't. It is about cloning genes, bits of DNA. Perhaps an analogy will make the difference more meaningful. An automobile is a rather complex machine assembled from many simple parts, some as simple as a screw. For each ...
Glossary Excerpted with modification from the Glossary in Genes V
Glossary Excerpted with modification from the Glossary in Genes V

... tested in pairwise combinations in trans; defines a genetic unit (the cistron) that might better be called a noncomplementation group. Conditional lethal mutations kill a cell or virus under certain (nonpermissive) conditions, but allow it to survive under other (permissive) conditions. Conjugation ...
Exam 3
Exam 3

... 4. The Hgh antibody will bind to the Hgh made by the colonies that contain the plasmid with the hgh gene. 5. Add a radioactive secondary antibody that binds to the primary antibody in step 4. 6. Detect the radioactive spot using autoradiography (exposing a piece of X-ray film). 7. Pick the colony th ...
Co-amplification of cytochrome b and D-loop mtDNA
Co-amplification of cytochrome b and D-loop mtDNA

Chromatin Structure 1
Chromatin Structure 1

Epigenetics of Cancer
Epigenetics of Cancer

... • Know some of the key epigenetic modifications that are important in disease • Understand the mechanisms that give rise to epigenetic alterations in disease • Understand how epigenetic alterations can be used as biomarkers • How epigenetic regulators can be targeted in disease ...
APPLICATION OF mtDNA TECHNOLOGY IN FORENSIC SCIENCE
APPLICATION OF mtDNA TECHNOLOGY IN FORENSIC SCIENCE

... • The DNA scientist was not at the scene and did not see the event happen • The DNA scientist must consider the possibility that the blood comes from someone other than the accused ...
Recombination in Bacteria Overview This module looks at how the
Recombination in Bacteria Overview This module looks at how the

... single stranded. This promotes recombination, as long as the DNA taken up is sufficiently homologous to the host DNA to allow recombination to occur. The recombination that occurs is one-way (non-reciprocal); unlike the exchange of strands diagrammed in the module on recombination, in this case the ...
Efficient whole-genome DNA methylation analysis of the Human
Efficient whole-genome DNA methylation analysis of the Human

... DNA methylation plays a critical role in gene regulation that influences normal organism development and many diseases including cancer. Profiling the DNA methylation patterns of higher organisms is challenging because methylation patterns vary between tissues and with developmental state, hence the ...
No Slide Title
No Slide Title

... Polypeptide chain = series of linked amino acids. AKA peptide chain. Arg ...
Astonishing DNA complexity demolishes neo-Darwinism
Astonishing DNA complexity demolishes neo-Darwinism

... as expected). Further study with more wide-ranging methods may raise this figure to 100%. Because much energy and coordination is required for transcription this means that probably the whole genome is used by the cell and there is no such thing as ‘junk DNA’. • Exons are not gene-specific but are ...
File - Intermediate School Biology
File - Intermediate School Biology

... To make Dolly, a cell was taken from the mammary tissue of a six-year-old sheep. Its DNA was added to a sheep ovum (egg) from which the nucleus had been removed. This artificially fertilised cell was then stimulated with an electric pulse and implanted in an ewe. (i) What is the difference between a ...
DNA, RNA, and Proteins
DNA, RNA, and Proteins

... —  Translation: The portion of protein synthesis that takes place at ribosomes and that uses the codons in mRNA molecules to specify the sequence of amino acids in polypeptide chains ...
Genetic Mutations
Genetic Mutations

... • Mutations can affect the reproductive cells of an organism by changing the sequence of nucleotides within a gene in a sperm or an egg cell. During what process would these types of mutations occur? • If this cell takes part in fertilization, the altered gene would become part of the genetic makeup ...
Chapter 13, 14 Rev
Chapter 13, 14 Rev

... a. No sugar is present in either molecule b. Hydrogen bonding is important only in DNA c. Only DNA has a backbone of sugars and phosphates d. Adenine pairs with different bases in DNA and RNA e. Thymine pairs with different bases in DNA and RNA Which of these is found in RNA but not in DNA? a. Adeni ...
DNA and RNA Chapter 12
DNA and RNA Chapter 12

... TRANSLOCATION Segment breaks off and joins a different non-homologous chromosome ...
Genetic Mutations
Genetic Mutations

... • Mutations can affect the reproductive cells of an organism by changing the sequence of nucleotides within a gene in a sperm or an egg cell. During what process would these types of mutations occur? • If this cell takes part in fertilization, the altered gene would become part of the genetic makeup ...
Go-ChIP-Grade™ Purified anti-Histone H3 (C
Go-ChIP-Grade™ Purified anti-Histone H3 (C

... *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engine ...
nucleotides - UniMAP Portal
nucleotides - UniMAP Portal

... little, if any, concoding DNA either between/ within gene sequences.  Gene expression - The regulation of many functionally related genes is enhanced by organizing them into operons. An operon is a set of linked genes that are regulated as a unit. ...
The Human Genome Project
The Human Genome Project

... Using hypersensitivity to DNaseI as a criterion, an international consortium of 442 scientists from 32 institutions has linked about 80% of the previously so-called “junk DNA” to some biochemical function. Such non-translated but functional DNA sequences include promoters, enhancers, sequences encod ...
Syllabus: AP Bio - Glen Ridge Public Schools
Syllabus: AP Bio - Glen Ridge Public Schools

... The course follows the description and outline proposed by the College Board. Advanced Placement serves students who wish to pursue college level studies in science while still in high school. Students are provided with the factual knowledge and conceptual framework as well as the conceptual skills ...
Organic Molecules Packet
Organic Molecules Packet

... Starches are very valuable because they provide a quick form of energy for the body. The second is glycogen. Glycogen is used for food storage in animals. The third is cellulose. Cellulose is used for structural support in plant cells. Sugars can be detected in foods through a simple lab test. To fi ...
< 1 ... 245 246 247 248 249 250 251 252 253 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report